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      • KCI등재

        A Basic Study on Marine Traffic Assessment in Mombasa Approach Channel-I

        Otoi, Onyango Shem,Park, Young-Soo,Park, Jin-Soo Korean Institute of Navigation and Port Research 2016 한국항해항만학회지 Vol.40 No.5

        Mombasa is the principle port of Kenya, serving inland countries in Eastern and central Africa. Mombasa port has undergone a massive infrastructure upgrade and dredging works with an expectation that more vessels and large post Panamax ships will be able to enter Mombasa port. Therefore, it is vital to carry out a marine traffic risk assessment in order to quantify the degree of navigation safety needed in the Mombasa approach channel and also to evaluate the navigation risk imposed on transit traffic by local ferry traffic. In this paper, a marine traffic risk assessment is carried out using the IWRAP mk2, Environmental Stress (ES) model, and the PARK model. Risk assessment results show that Likoni area has an unacceptable stress/risk ranking at 20.7% by the ES model and 38.89% by the PARK model. The IWRAP mk2 model shows that the crossing area has the highest risk of crossing collision and the area at the entrance to the inner channel has a high risk of grounding. The conclusions derived from this study will provide the basis for proposing the most effective countermeasure to improve navigation safety in the Mombasa approach channel.

      • KCI등재

        A Basic Study on Marine Traffic Assessment in Mombasa Approach Channel-I

        Onyango Shem Otoi,Young-Soo Park,Jin-Soo Park 한국항해항만학회 2016 한국항해항만학회지 Vol.40 No.5

        Mombasa is the principle port of Kenya, serving inland countries in Eastern and central Africa. Mombasa port has undergone a massive infrastructure upgrade and dredging works with an expectation that more vessels and large post Panamax ships will be able to enter Mombasa port. Therefore, it is vital to carry out a marine traffic risk assessment in order to quantify the degree of navigation safety needed in the Mombasa approach channel and also to evaluate the navigation risk imposed on transit traffic by local ferry traffic. In this paper, a marine traffic risk assessment is carried out using the IWRAP mk2, Environmental Stress (ES) model, and the PARK model. Risk assessment results show that Likoni area has an unacceptable stress/ risk ranking at 20.7% by the ES model and 38.89% by the PARK model. The IWRAP mk2 model shows that the crossing area has the highest risk of crossing collision and the area at the entrance to the inner channel has a high risk of grounding. The conclusions derived from this study will provide the basis for proposing the most effective countermeasure to improve navigation safety in the Mombasa approach channel

      • SCIESCOPUSKCI등재

        The Effects of Donor Cell Type and Culture Medium on in vitro Development of Domestic Cat Embryos Reconstructed by Nuclear Transplantation

        Fahrudin, Mokhamad,Otoi, Takeshige,Karja, Ni Wayan Kurniani,Murakami, Masako,Suzuki, Tatsuyuki Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.8

        In this study we explored the possibility of performing nuclear transfer in the domestic cat and assessed the ability of different culture media to support in vitro development of reconstructed cat embryos. Donor somatic cells were derived from cultured cumulus cells or explants of oviduct tissue, and recipient cytoplasts from in vitro matured oocytes. A higher percentage of cleavage (84.6% and 86.5%) and development to the morula stage (35.9% and 44.2%) was found when reconstructed embryos receiving cumulus or oviduct cells were cultured in MK1 medium, compared with those cultured in CR1aa (58.7% and 72.5%, 13.8% and 13.6%, respectively). There was no significant difference between MK1 and CR1aa media with respect to the proportion developing to the blastocyst stage (15.4% and 17.3% vs 6.8% and 8.6%, respectively, p>0.05). There was no significant effect (p>0.05) of donor cell type (cumulus and oviduct cells) on the rates of fusion (65.0% and 52.5%), cleavage (84.6% and 86.5%), development to the morula (35.9% and 44.2%), and blastocyst (15.4% and 17.3%) stages when reconstructed embryos were cultured in MK1 medium. Similar results were found for the reconstructed embryos cultured in CR1aa medium. These results show that culture medium has a significant impact on the early development of reconstructed cat embryos, whereas donor cell type does not have a significant effect.

      • KCI등재

        Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows

        Urara Watanabe,Mitsuhiro Takagi,Osamu Yamato,Takeshige Otoi,Koji Okamoto 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.2

        This retrospective study was conducted to confirm therelationship between pre- and postpartum metabolicparameters and postpartum reproductive performance and toclarify seasonal characteristics of the metabolic parameters byusing our metabolic profile test (MPT) database of JapaneseBlack breeding herds. In evaluation 1, MPT databases of bloodsamples from multiparous cows collected prepartum andpostpartum were divided into two groups according to calvinginterval, and each MPT parameter was compared. Inevaluation 2, the same MPT databases used in evaluation 1 weredivided into two groups according to the sampling period. Significant differences were found in the prepartal total proteinand postpartal γ-glutamyltransferase in evaluation 1. Inevaluation 2, significant differences were found in the prepartaland postpartal total protein, albumin/globulin ratio, andglucose. Clear seasonal differences in MPT results emphasizedthe usefulness of the MPT in breeding cattle herds fedhome-pasture roughage and suggest that unsatisfactoryreproductive performance during hot periods reflectsinadequate nutritional content of the diet and possible reducedfeed intake due to heat stress.

      • SCIESCOPUSKCI등재

        Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable $CO_2$ Incubator

        Dong, Y.J.,Bai, X.J.,Varisanga, M.D.,Mtango, N.R.,Otoi, T.,Rajamahendran, R.,Suzuki, T. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.2

        The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20$-20^{\circ}C$, $-30^{\circ}C$ or $-80^{\circ}C$ in the presence of 5% DMSO used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstracted from the somatic cells, 62.3%, 76.6% to 65% showed cleavage 70.5%, 81.9% to 78.5% reached the stage of morula formation and 39.7%, 43.2% or 47.6% reached the blastocyst stage. There was no significant difference in development when the NT embryos were compared with those reconstracted from fresh somatic cell derieved skin tissues (72%, 75.3%, and 45.2%, for cleavage, and development to morula and blastocyst stage, respectively). NT embryos were then placed in a portable $CO_2$ incubator and carried to China from Japan by air. After reaching to farm, two NT embryos were transferred to each of 5 recipients. We obtained 2 NT calves which birth weights is 30kg and 36kg female, and gestation periods is 281 and 284 days, respectively. There were no observation any abnormality from those calves. The results indicated that cell lines derieved from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer using the portable $CO_2$ incubator.

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