DNA 포렌식에 의한 DNA 증거의 증거능력에 관한 고찰

김동준(Kim, Dong­Jun),오경식(Oh, Kyung­Sik) 한국피해자학회 2018 被害者學硏究 Vol.26 No.1

Advances in forensic DNA analysis have led to tremendous improvements in identifying and confirming perpetrators and victims. This has added credibility to DNA evidence, resulting in cases where criminals are more likely to be forced to own up to their criminality and culpability. This paper addresses scientific and technical theories the DNA analytical technologies are founded on as well as the suitability of individual analytical procedures. Beyond these issues, it went on to discuss the requirements that need to be met in order for DNA evidence, collected through forensic DNA testing, to be admissible in court in Korea. DNA testing results are presented in court in the form of certified expert testimony. And according to Article 313, “Statement, etc.” of the Criminal Procedure Act, as long as the DNA evidence offered is free from errors in laboratory procedures and concluding interpretations of the person responsible for producing the expert opinion recording, such procedures and outcomes will be admissible as legitimate evidence in court. In other words, the aforementioned expert testimony is nothing short of legislative intent at acknowledging the testimony as admissible in court, thus should not be understood as standards with which the scientific evidence concerned is admissible and tried at court. For this reason, admissibility in court of DNA evidence from DNA forensic analysis should not only be based on whether it meets the requirements as expert testimony, but also whether its theories of reasoning and analyses are founded on acceptable scientific principles. Furthermore, the tools and methods used in DNAprofiling need to be validated, allowing the analyzed result in question to be assessed for admissibility in criminal court. Trends of precedents regarding DNA evidence seem to require that the relevancy of DNA analytical theories, suitability of DNA analytical methods that have implemented these theories, and maintenance of the chain of custody of evidence by applying scientific procedures to the targets concerned, be met for the evidence to be admissible in court. Furthermore, it may be fair to say that within the structure of Korea’s criminal prosecution, the processes involved in determining whether or not DNA evidence is credible may be part of what is needed to assess its probative value. At the moment, theoretical foundations of DNA analysis are deemed reliable with the overall approval of scientific circles, however there are improvements made all the time in standard analytical methodologies and procedures regarding their implementation and analytical techniques. This has created an unbridgeable gap between common knowledge and the outcomes of scientific research activities that legal experts, particularly trial judges, are left with no choice but to be more and more dependent on expert testimony when they assess the credibility of DNA evidence. Still, the more weight DNA analysis carries, the more likely serious errors in laboratory testing occur; thus, there is increased emphasis on how to keep the chain of custody in determining the admissibility of DNA evidence, for example, the qualification of those involved in testing, sample collection and preservation, stability of the testing methods that have been utilized, and conformance to analytical procedures. Therefore, if there is evidence that is crucial in determining guilt in crime, and the continuity of its preservation is closely related to the presence and integrity of the evidence concerned, namely DNA evidence, the defendant should be afforded the opportunity for the credibility of evidence to be tried in court during the entire processes of such evidence being collected, stored and analyzed to: prevent wrongful accusations; to make court proceedings more impartial; and to comply with appropriate procedures. To this end, cross examination of witnesses, disclosure of extensive evidence of the parties involved, and cross examinat

A Study of Efficient Connection using Mobility Property in Mobile Ad-hoc Network

Youngj-jun Oh1,Kang-whan Lee 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.12

In this paper, we proposed the context-awareness routing algorithm DDV (Dynamic Direction Vector)-hop algorithm at Mobile Ad-hoc Networks. Existing algorithm in MANET, it has a vulnerability that the dynamic network topology and the absence of network expandability of mobility of nodes. This proposed algorithm forms cluster formation using a range of direction and threshold of velocity by base-station, and we calculate exchange cluster head node probability using direction and velocity for maintaining cluster formation. DDV algorithm forms a cluster based on the cluster head node. As a result of simulation, our scheme could maintain the proper number of cluster and cluster members regardless of topology changes. In response to maintain cluster formation, we prove a continuous connection between nodes.

Scanning tunneling microscopy investigation of nano-structured α-K5PW11(M x OH2)O39(M = Mn(II), Co(II), Ni(II), and Zn(II)) Keggin heteropolyacid catalyst monolayers.

Choi, Jung Ho,Kang, Tae Hun,Bang, Yongju,Yoo, Jaekyeong,Jun, Jin Oh,Song, In Kyu American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.11

<P>Nano-structured α-K5PW11(M x OH2)O39 (M = Mn(II), Co(II), Ni(II), and Zn(II)) Keggin heteropolyacids (HPAs) were investigated by scanning tunneling microscopy (STM) and tunneling spectroscopy (TS) measurements in order to elucidate their redox property and oxidation catalysis. HPA molecules formed two-dimensional self-assembled monolayer arrays on highly oriented pyrolytic graphite (HOPG) surface. Furthermore, HPAs exhibited a distinctive current-voltage behavior referred to as negative differential resistance (NDR) phenomenon. The measured NDR peak voltage of HPAs was correlated with the reduction potential and the absorption edge energy determined by electrochemical method and UV-visible spectroscopy, respectively. NDR peak voltage of HPAs appeared at less negative voltage with increasing reduction potential and with decreasing UV-visible absorption edge energy. The correlations strongly suggested that NDR phenomenon was closely related to the redox property of HPAs. Vapor-phase oxidation of benzyl alcohol to benzaldehyde was carried out as a model reaction to track the oxidation catalysis of HPAs. NDR peak voltage appeared at less negative voltage with increasing yield for benzaldehyde.</P>

Identification and characterization of Dunaliella salina OH214 strain newly isolated from a saltpan in Korea

Minjae, Kim,Hyeon Jun, Oh,Khanh, Nguyen,EonSeon, Jin The Korean Society of Phycology 2022 ALGAE Vol.37 No.4

Carotenoids are effective antioxidants that are found in various photosynthetic organisms. Marine microalgae are an advantageous bioresource for carotenoid production because they do not compete with other crops for freshwater and arable land. This study reports a newly isolated Dunaliella strain from the Geumhong Saltpan on Yeongjong Island, West Sea, Korea. The new strain was isolated and classified as Dunaliella salina through phylogenetic analysis and was named the OH214 strain (Deposit ID: KCTC14434BP). The newly isolated strain can survive in a wide range of NaCl concentrations (0.3-5.0 M NaCl), but grows well in 0.6 to 1.5 M NaCl culture medium. Under high-light conditions (500 ± 10 μmol photons m^-2 s^-1), the cells accumulated three times more β-carotene than under low-light conditions (50 ± 5 μmol photons m^-2 s^-1). The cells accumulated 2.5-fold more β-carotene under nitrogen-deficient (1 mM KNO₃) conditions (3.24 ± 0.36 ㎍ 10⁶ cells^-1) than in nitrogen-sufficient conditions (>5 mM KNO₃). The lutein content under nitrogen-deficient conditions (1.73 ± 0.09 ㎍ 10⁶ cells^-1) was more than 24% higher than that under nitrogen-sufficient conditions. Under the optimized culture condition for carotenoid induction using natural seawater, D. salina OH214 strain produced 7.97 ± 0.09 mg g DCW^-1 of β-carotene and 4.65 ± 0.18 mg g DCW^-1 of lutein, respectively. We propose that this new microalga is a promising strain for the simultaneous production of β-carotene and lutein.

Laminar Lifted Methane Jet Flames in Co-flow Air

Narayan P. Sapkal,Won June Lee,Jeong Park,Byeong Jun Lee,Oh Boong Kwon 한국연소학회 2015 KOSCOSYMPOSIUM논문집 Vol.2015 No.12

The Laminar lifted methane jet flames diluted with helium and nitrogen in co-flow air have been investigated experimentally. The chemiluminescence intensities of OH* and CH₂O* radicals and the radius of curvature for tri-brachial flame were measured using an intensified charge coupled device (ICCD) camera, monochromator and digital video camera. The product of OH* and CH₂O* is used as a excellent proxy of heat release rate. These methane jet flames could be lifted in buoyancy and jet dominated regimes despite the Schmidt number less than unity. Lifted flames were stabilized due to buoyancy induced convection in buoyancy-dominated regime. It was confirmed that increased OH* and CH₂O* concentration caused an increase of edge flame speed via enhanced chemical reaction in buoyancy dominated regime. In jet momentum dominated regime lifted flames were observed even for nozzle exit velocities much higher than stoichiometric laminar flame speed. An increase in radius of curvature in addition to the increased OH* and CH₂O* concentration stabilizes such lifted flames.

Lysophosphatidic acid increases mesangial cell proliferation in models of diabetic nephropathy via Rac1/MAPK/KLF5 signaling

Kim, Donghee,Li, Hui Ying,Lee, Jong Han,Oh, Yoon Sin,Jun, Hee-Sook Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.2

<▼1><P>Mesangial cell proliferation has been identified as a major factor contributing to glomerulosclerosis, which is a typical symptom of diabetic nephropathy (DN). Lysophosphatidic acid (LPA) levels are increased in the glomerulus of the kidney in diabetic mice. LPA is a critical regulator that induces mesangial cell proliferation; however, its effect and molecular mechanisms remain unknown. The proportion of α-SMA<SUP>+</SUP>/PCNA<SUP>+</SUP> cells was increased in the kidney cortex of <I>db/db</I> mice compared with control mice. Treatment with LPA concomitantly increased the proliferation of mouse mesangial cells (SV40 MES13) and the expression of cyclin D1 and CDK4. On the other hand, the expression of p27<SUP>Kip1</SUP> was decreased. The expression of Krüppel-like factor 5 (KLF5) was upregulated in the kidney cortex of <I>db/db</I> mice and LPA-treated SV40 MES13 cells. RNAi-mediated silencing of KLF5 reversed these effects and inhibited the proliferation of LPA-treated cells. Mitogen-activated protein kinases (MAPKs) were activated, and the expression of early growth response 1 (Egr1) was subsequently increased in LPA-treated SV40 MES13 cells and the kidney cortex of <I>db/db</I> mice. Moreover, LPA significantly increased the activity of the Ras-related C3 botulinum toxin substrate (Rac1) GTPase in SV40 MES13 cells, and the dominant-negative form of Rac1 partially inhibited the phosphorylation of p38 and upregulation of Egr1 and KLF5 induced by LPA. LPA-induced hyperproliferation was attenuated by the inhibition of Rac1 activity. Based on these results, the Rac1/MAPK/KLF5 signaling pathway was one of the mechanisms by which LPA induced mesangial cell proliferation in DN models.</P></▼1><▼2><P><B>Kidney disease: Mechanisms of diabetes-related damage</B></P><P>A potent molecular mediator of diabetic kidney disease induces its pathogenic effects via proteins that could be targeted with future drug therapies. Yoon Sin Oh
from Eulji University in Seongnam-si and Hee-Sook Jun from Gachon University in Incheon, both in South Korea, and colleagues treated certain cells found in the kidney’s glomerulus, the organ’s filtering unit, with a signaling molecule called lysophosphatidic acid (LPA) that is elevated in the blood of diabetic mice. They showed that LPA stimulated cellular proliferation and boosted the expression of proteins involved in regulating the cell cycle and a multipurpose signaling pathway. They then inhibited the activity of these proteins to prevent the kidney cells’ hyperproliferation, both in cell culture and in diabetic mice. The results highlight the potential of blocking mediators of LPA signaling to treat kidney-related complications of diabetes.</P></▼2>

이동로봇의 실시간 장애물인식에 관한 연구

오세봉,Nguyen Huu Cong,김준홍,조창제,한성현 한국공작기계학회 2008 한국공작기계학회 추계학술대회논문집 Vol.2008 No.-

This paper achieves a research about avoidance of obstacles avoidance based on ultrasonic sensor. The performance of the proposed obstacle avoidance robot controller in order to determine the exact dynamic systems modeling system that uncertainty is difficult for nomadic controlled robot direction angle by ultrasonic sensors throughout controlled performance tests. In additionally, this study is an in different ways than the self-driving simulator in the development of ultrasonic sensors and unmanned remote control techniques used by the self-driving robot controlled driving through an unmanned remote controlled unmanned realize the performance of factory automation.

연장 가골에 골 이식 대체물 투여가 골 경화에 미치는 영향 : 가토 경골에서의 방사선 및 골 밀도의 변화 Changes of Radiography & Bone Mineral Density in the Tibia of Rabbits

오창욱,김풍택,박병철,송해룡,박일형,백준호,박형진 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

목적 : 골 연장술 후 생기는 연장 가골에 황화 칼슘과 이종 이식골을 투여하여 골 경화 속도에 대한 영향을 알아보기 위한 실험이었다. 연구대상 및 방법 : 성장중인 뉴질랜드 화이트 토끼(2.0-2.5㎏)의 경골 간부에 골막을 보호하고, 절골술을 시행하고, 5일간의 휴지기후 외고정 장치를 이용하여 1주일 동안 7㎜가 연장될 때까지 하루 1㎜씩 2회의 리듬을 가지고 골 연장을 실시하였다. 제1 실험 군 7마리에는 연장이 끝난 직후 황화 칼슘(Osteoset^(ⓡ), Wright medical USA) 1정, 제 2 실험 군 7마리에는 이종 이식골의 일종인 Lubboc 5㎟을 각각 연장된 가골 부에 주입하고, 단순 연장한 대조군 7마리와 함께, 방사선 및 골 밀도비를 측정하고 추시하여, 골 경화의 진행속도를 비교하였다. 방사선 촬영은 전후면 및 측면사진을 1주 간격으로 촬영하였고, 골밀도 측정은 DEXA(dual energy X-ray absorptionmetry, Lunar^(ⓡ)) 장치를 2주일 간격으로 시행하였다. 결과 : 방사선 소견상, 제 1실험군의 연장가골의 완전 경화시간은 평균 14주, 제 2실험군은 154 주로 각각 대조군의 16.9주보다 단축되었으며, 골 밀도 비에서도, 두 실험군에서 최고치가 대조군의 값보다 높고, 최고치까지의 경과기간도 빨랐다. 결론 : 본 연구에서 연장 가골에 황화칼슘 및 이종 이식골의 투여가 골 경화의 기간을 단축시킬 수 있고, 외고정 장치의 빠른 제거에 도움이 될 수 있음을 알았다. Purpose : This study was designed to know the effect of calcium-sulfate and xenograft on the distracted callus after lengthening. Materials & Methods : We had operation of subperiosteal osteotomy and external fixation on the tibial diaphysis of young New Zealand White rabbits(2.0-2.5㎏); after 5 days of latency period, 7㎜(1㎜/day, 2times/day) of tibial lengthening was reached in a week. At 1 week after lengthening, the 1st experimental group of 7 rabbits received a pellet of calcium sulfate(Osteoset, Wright medical, USA) in the distraction gap, and the 2nd experimental group of 7 rabbits received 5㎜ 2 of xenogrfat(Lubboc) in the distraction gap. But, the control group of 7 rabbits did not receive any of above materials. We compared three groups with the changes of radiographic findings at every week and bone mineral ratio(DEXA) at every two weeks. Results : The time to complete consolidation of distraction callus of both experimental group(calcium sulfate;14 weeks, xenograft; 15.4 weeks) was shorter than that of control group(16.9 weeks) in radiographic findings. Maximum value of bone mineral ratio of distraction callus was higher and the time to reach the highest value was also shortened in the both experimental group compared to control group. Conclusion : By use of bone substitutes as like calcium sulfate or xenograft in the distraction callus with external fixator, it may be possible to shorten the consolidation period and the fixator-wearing period.

알코올 사용과 스트레스와의 관련성

전진용,오동열,구민성,이준석,천근아,박웅섭,김상아 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.4

Objectives : This study was designed to assess the relationship between alcohol use and stress. Methods : The study subjects were 1,261 people who drink among the 2,565 people who lived in Gangneung. We investigated the sociodemographic characteristics, Short Form-12 (SF-12), Global Assessment of Recent Stress (GARS) and Alcohol Use Disorder Identification Test (AUDIT). Results : In Problem drinking group the GARS was significantly positively associated with the AUDIT (p=0.02). In Social drinking group sex (p<0.001) and age (p=0.02) is significantly associated with the AUDIT. Conclusion : This study showed the significantly positively association between stress and alcohol use. We think that the intervention and active management of the stress can prevent the alcohol problem.

폐목재로 제조된 파티클보드의 성능평가

오용성,차재경,곽준혁 한국목재공학회 2002 목재공학 Vol.30 No.4

현재 폐목재의 발생량은 국내의 연간 목재사용량의 40% 정도의 수준으로 국내의 목재자급율을 고려해볼 때, 목재패널산업에서의 이런 폐목재의 재활용은 많이 장려되어야 한다. 본 연구는 폐목재로부터 얻어진 파티클과 실험실에서 합성한 요소수지를 폐목재파티클의 전건무게에 대해 6% 첨가하여 열압온도 162℃와 열압시간 5분에서 파티클보드를 제조하고 ASTM D 1037 방법에 의해 파티클보드의 성능을 평가하였다. 파티클보드의 성능을 평가한 결과는 KS F 3104 파티클보드 8.0형의 최소요구를 만족시키는 것이다. 이런 파티클보드의 성능평가 결과는 폐목재가 파티클보드의 원료로 적합하다는 것을 보여준다. The generation of wastewood is currently about 40% level of the amount of domestic wood use. When the rate of wood self-supply is considered, the recycling of wastewood in wood panel products industry should be encouraged. This study was evaluated the performance of particleboard (PB) made from wastewood. PBs were manufactured with the synthesized urea-formaldehyde resin at 5 minutes hot-press time, and at 162℃ press temperature with 6 percent resin solids level on an ovendry wastewood particle weight basis. The PBs were tested by the procedure of ASTM D 1037. Test results exceeded the minimum strength requirement according to KS F 3104 Particleboard type 8.0. The PB's performance showed that wastewood is suitable raw material for PB.