數種의 生藥煎劑가 白舅 臟器內 Transaminase 活性度에 미치는 影響

安年衡,申東于 圓光大學校 1993 論文集 Vol.27 No.2

The effect of Decoction on transaminase in the rat was estimated and the activity of transaminase determined by spectrometric method at 505nm. Artemisiae Messer schmidtianae Herba, Bupleuri Radix, Gardeniae Fructus, Aurantii Nobilis percapium and Scutellariae Radix significantly increased transaminase activity in the liver. Also in the kidney, it was increased by Polyporus, Alismatix Rhizoma, Junci Herba. Respectively Trichosanthis Radix, Acorus Graminens,Thujae semen, Polygalae Radix increased the activity of transaminase in the heart.

표면 플라즈몬 공명형 Sucrose 센서의 제작 및 특성

엄년식,고광락,김재호,강신원 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1

A surface plasmon resonance (SPR) sensor for determination of sucrose concentration using gold thin film was constructed. The properties of gold thin film are very serious factors in exciting surface plasmon resonance. So the optimum condition of gold thin-film for the SPR phenomenon was investigated. The result is that the optimum thickness is 545Å at 43.75° resonance angle and roughness is about 3A at that time. Using this SPR system, we got the characteristic of the linear resonance angle shift from 10 wt% to 40 wt% sucrose concentrations.

統合的인 思考方法을 育成하기 爲한 算數指導의 事例와 그考察

韓年鍾 群山敎育大學 1974 論文集 Vol.7 No.-

In the integrated thought children do not grasp the objects of arithmetic, such as recognized or conjectured experiences, fittings, surrounding, knowledge, functions, principles, formulae, conceptions, terms, signs, techniques, as separated ones individually but grasp them as a whole object in connection with the already acquired objects in common. And they also think how the individual object works and functions in the inclusive object. In this paper I try to study on the fundamental teaching conditions for developing the integrated thought and thought patterns formed by it. The contents of this thesis are as follows; 1. The meaning of the integrated thought 2. The fundamental teaching conditions 3. The thought patterns formed by the integrated thought The integrated thought is not a quite new approach in teaching arithmetic. Being used in the past it was not taken serious. Nowadays, theachers are interested in it more than ever in connection with the modernization of arithmetic teaching. I think teachers require various concrete examples for teaching it with confidence. That's the reason why I aim to study this paper.

ETHYNODIOLDIACETATE의 대사에 관한 연구(Ⅱ)

安年衡 圓光大學校 1986 論文集 Vol.20 No.2

Ethynodioldiacetate의 대사가 토끼의 간장, 신장, 혈액, 피부, 질에서 어떻게 이루어지는가를 시험관내에서 조사 비교하였다. Ethynodioldiacetate is metabolized by various rabbit tissues to norethindrone. A study was undertaken to determine in vitro metabolic norethindrone pattern of ethynodioldiacetate. Rabbit liver, kindney, blood an vagina were used as enzyme source.

돼지에 있어서 Sphingosine과 Sphinganine의 함량에 미치는 Fumonisin의 영향

安年衡 圓光大學校 1992 論文集 Vol.26 No.2

The fumonisins(FB) are mycotoxins produced by Fusarium moniliforme. FB₁induced pulmonary edema, liver and pancreatic damage, and death in swine. We examined dose-response features of FB evaluating morphological changes in liver and sphingolipid metabolism in liver and serum. Thirty 6-14kg male weanling pigs were randomly assigned to 6 test diets. Diets 1-4 contained 60%, 30%, 15% or 7.5% corn screening that were contaminated with FB₁at 166ppm and FB₂at 48ppm:diet 5 contained "clean" screenings(FB₁at 6.4ppm) and diet 6 ground corn(FB₁at 3ppm). Pigs were euthanized on day 14 or when respiratoty distress was observed on day 4-7(3/5 pigs on dietll and 1/5 on diet 2). Hepatocyte disorganization, single cell necrosis and mild inflammation were present in all pigs on diet 1 and 2, 3/5 on diet 3 and 1/5 on diet 4. The levels of sphingosine and sphingaine were elevated in serum and liver from pigs fed fumonisin-contaminated feed. The sphinganine to sphingosine ratios were 10 folds in liver and 6 folds in serum compared to the level immediately prior to feeding fumonisin.

Ethynodioldiacetate의 대사에 대한 연구

安年衡 圓光大學校 1983 論文集 Vol.17 No.2

Ethynodiol diacetate는 경구 피임약으로 널리 사용되어 왔으며 이에 대한 硏究로는 tritated ethynodiol diacetate를 암쥐에 體重 100g당 0.1 mg의 量을 투여한 후 각장기내의 radioactivity의 분포를 관찰한 결과 특이한 선택성이 없음을 보고했고 (Watanabe et. al,1968), 쥐에 있어서는 Deacetylation되어 ethynodiol을 거쳐 간에서 Norethindrone으로 전환된다는 보고 (Tokuda et al, 1967)와 生體內에서 17α-ethynylestradiol로 전환된다는 보고(Besch et al. ,1963)가 있으며 여성에게 Ethynodioldiacetate를 경구투여 후 糞尿에 存在하는 이의 代謝産物에 分離確認되고 (Kishimoto et al.,1972)혈장에 存在하는 대사산물도 분리 확인되었으며 (Cook et al.,1973)쥐와 사람의 肝을 사용하여 Ethynodiol diacetacetate의 시험관내 대사실험으로 대사산물도 분리확인 되었다. (Freudenthal et al.,1971) 이외에도 Ethynodioldiacetate와 이의 대사산물의 분리 확인 방법이 다수 보고 되어 있다.(Walls et al.,1977) 이와 같이 ethynodioldiacetate의 대사에 대한 연구는 동물이나 사람의 간이나 혈장내에서는 이루어졌지만 피부에 있어서는 아직 보고된 바가 없으며 부신피질 홀몬을 비롯하여 몇몇의 steroid 홀몬이 피부에서 이루어지는 대사에 대해서는 많이 보고된 점에 착안하여 토끼와 Hairless mouse의 피부에서 ethynodioldiacetate가 어떻게 대사되는가를 연구한 결과 약간의 지견을 얻었기에 이에 보고하는 바이다. Ethynodioldiacetate is metabolized by both hairless mouse and rabbit skin to a number of intermadiary metabolites. A sensitive, specipic high-performance Liquid chromatographic procedure for the determination of Norethindrone in animal skin is described. The organic solvent extract of metabolites of Ethynodiol diacetate in animal skin is chromatographed on a reversed phase column using a high-performance liquid chromatograph fitted with an ultraviolet detector(210nm) The system was gradient acetonitrile 65~90%(v/v) in water as mobile phase on a 150×4.6mm I.D.RP-8-10㎛ colum. Metabolites and endogenous substances do not interfere with the assay of Nore thindrone.

광도파로형 표면 플라즈몬 공명 센서 소자의 제작 및 그 특성

조동헌,엄년식,장수원,권대혁,강신원 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

Prednisolone의 細胞性免疫反應에 대한 Leucocyte Adherence inhibition의 硏究

姜恩美,安年衡 圓光大學校 1990 論文集 Vol.24 No.2

Prednisolone의 免疫 抑制 효과를 究明하기 위해 緬羊 赤血球를 抗原으로 하여 mouse를 免疫시키고 免疫前後에 prednisolone 40㎎/㎏을 일정 시간 간격으로 복강내에 투여하였다. 복강내로 삼출된 白血球 付着 抑制 實驗(LAI)과 mouse체중당 복胸腺과 脾臟의 重量化를 (%) 구한 결과는 1. prednisolone을 免疫 1日前과 免疫과 同時에 投與했을때 LAI는 有意하게 低下되었으나 免疫後에는 약간 低下되었다. 2. mouse체중당 胸腺과 脾臟의 重量化(%)는 免疫 1日前과 免疫後, 免疫과 同時에 有意하게 低下되었으나, 免疫 3日後에는 약간 저하되었다. 3. prednisolone投與後 일정 시간 간격으로 LAI를 측정한 결과 30分과 120分後에는 低下되었으나 60分과 180分後에는 증가되었다. 이상의 결과에서 免疫 1日前과 免疫과 同時에 prednisolone을 投與했을때 細胞性 免疫 反應이 가장 抑制됨을 볼 수 있었다. This study was designed to determine the immunosuppressive effect of prednisolone on the mouse by the leucocyte adherence inhibition(LAI) assay and the proportin of thymus weight and spleen weight per body weight. The mice were immunized with the sheeo red blood cell and received a intraperitoneal dose of 40㎎/㎏ of prednisolone at various inteval days before or after immunization. Lymphocyte was drawn from the peritoneal exudated cell of mice. The results were as follows. 1. LAI was significantly decreased when prednisolone was injected 1 day before or at the same time of immunization, but slightly after immunization. 2. The proportion thymus weight and spleen weight per body weight were significantly decreased when prednisolone was injected 1 day before, after and at the same time of immunization, but slightly 3 days after immunization. 3. LAI was determined on various interval times after prednisolone injection. It was decreased on 30 and 120 mins, but increased on 60min and 180 mins. Thease results indicated that the cell-mediated immune response of prednisolone was suppressed when it was injected 1 day before and at the same time immunization.

대식세포 NAD glycohydrolase에 관한 연구

이지영,안년형 圓光大學校 1995 論文集 Vol.30 No.2

NAD glycohydrolase (NADase:EC 3.2.2.5)는 포유류에서 형질막에 glycosylphosphatidylinositol (GPI) 결합으로 연결되어 있어 박테리아의 phosphatidylinositol-특이성 phospholipase C (PI-PLC)로 가용화 된다. 본 연구에서는 결핵 감염된 마우스로 부터 대식세포를 얻어 LPS처리후 막외 NADase가 유리되는 것을 관찰하였다. 이 활성화된 대식세포에서 NADase가 유리되기에 앞서서 대식세포에 내재하는 GPI-PLC 활성이 증가하는 사실을 관찰하였다. 이는 대식세포의 활성화에 의해 대식세포에 내재하는 GPI-PLC활성이 증가하므로써 GPI결합으로 붙어 있는 NADase가 유리됨을 의미한다. 활성화 대식세포에서 증가된 GPI-PLC 활성 분획을 DEAE column 크로마토그라피를 시행하여 단일 peak의 용출액을 얻은 후 이분획에서 활성을 확인한 결과 GPI-PLC 활성은 없음을 알았다. NAD glycohydrolase (NADase) is located on the surface of the cell via glycosylphosphatidylinositol (GPI)-linkage, which can be cleaved by bacterial PI-specific phospholipase C (PI-PLC). When macrophages primed with live or dead Mycobacterium tuberculosis were treated with liposaccharide, ecto-NADase was released from activated macrophages. The result that the release of NADase followed the increase of GPI-PLC activity in macrophages suggests that endogenous GPI-PLC can cleave the PI-linkage of NADase on the macrophages. The GPI-cleavae activity was shown as a single peak on DEAE chromatography and the activity was not inhibited by phenanthroline, a selective inhibitor of GPI-phospholipase D (GPI-PLD). There data indicate that the release of NADase from activated macrophages may be due to the activity of GPI-PLC, but not GPI-PLD.

폴리카보네이트 브라켓 부착 시 결합강도 증진을 위한 표면처리 효과

김석필,김년경,이현정,황현식 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.5

본 연구는 폴리카보네이트 브라켓 부착 시 표면처리가 결합강도를 증진시키는지 알아보고자 시행되었다. 소의 하악 중절치를 포매하여 만든 100개의 시편에 광중합형 레진 접착제를 이용하여 폴리카보네이트 브라켓(Alice, 광명 데이콤, 대한민국)을 부착하였다. 대조군의 경우, 표면처리 없이 부착한 반면, 실험군의 경우 샌드블라스팅 처리한 경우, plastic conditioner 처리하여 부착한 경우, 그리고 샌드블라스팅과 함께 plastic conditioner 처리한 경우로 구분하여 접착하였다. 만능시험기를 이용하여 전단결합강도를 측정하고 파절양상을 비교 분석한 결과, plastic conditioner나 샌드블라스팅으로 표면처리를 시행한 경우 표면처리를 시행하지 않은 경우에 비해 높은 결합강도를 보였다 (p<0.001). 샌드블라스팅과 Plastic conditioner를 모두 처리한 경우는 샌드블라스팅만이나 plastic conditioner만 처리한 경우보다 높은 결합강도를 보였으며, 특히 샌드블라스팅만 처리한 경우에 비해서는 통계적 유의차를 보였다 (p<0.05). 이상의 연구결과는 폴리카보네이트 브라켓 부착 시 결합강도 증진을 위해서 베이스의 표면처리가 필요하며 샌드블라스팅 후 plastic conditioner 도포가 가장 효과적임을 보여주었다. Objective: The purpose of this study was to evaluate whether the bond strength of polycarbonate brackets can be increased through surface treatment. Methods: One hundred polycarbonate brackets (Alice) were bonded to bovine incisors with light-cured adhesive. The bracket bases were treated with one of three methods; sandblasting, plastic conditioner application, and combined treatment with sandblasting and plastic conditioner. The brackets without any surface treatment served as the control. The shear bond strength was tested with a universal testing machine, and failure pattern was assessed with the adhesive remnant index. Results: The shear bond strength in all experimental groups was higher than that of the control group (p<0.001). The group treated with plastic conditioner after sandblasting showed statistically higher shear bond strength than the sandblasting only group (p<0.05). The group treated with plastic conditioner after sandblasting showed higher shear bond strength than plastic conditioner only group, but the difference was not statistically significant. Conclusion: The above results suggest that the surface treatments of polycarbonate bracket is mandatory to improve bond strength, and the most effective method is an application of plastic conditioner after sandblasting.