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Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
Ningsheng Ruan,Chensheng Lin,Xiuqing Dong,Xuefeng Hu,Yanding Zhang 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.2
Fast progresses in stem cell-based tooth tissue engineering have been achieved in recent years in several animal models including the mouse, rat, dog, and pig. Moreover, various postnatal mesenchymal stem cells of dental origin have been isolated and shown capable of differentiating into odontoblasts and generating dentin. Meanwhile, human ker- atinocyte stem/progenitor cells, gingival epithelial cells, and even iPSC-derived epithelium have been demonstrated to be able to differentiate into functional ameloblasts. Translational medicine studies in the nonhuman primate are irreplaceable steps towards clinical application of stem cell-based tissue engineering therapy. In the present study, we first examined the epithelial stem cell markers in the rhesus skin using immunostaining. Keratinocyte stem cells were then isolated from rhesus epidermis, cultured in vitro, and characterized by epithelial stem cell markers. Epithelial sheets of these cultured keratinocytes, which were recombined with E13.5 mouse dental mesenchyme that possesses odontogenic potential in the presence of exogenous FGF8, were induced to differentiate into enamel-secreting ameloblasts. Our results demonstrate that in the presence of appropriate odontogenic signals, rhesus keratinocytes can be induced to gain odontogenic competence and are capable of participating in odontogenesis, indicating that rhesus keratinocytes are an ideal epithelial cell source for further translational medicine study of tooth tissue engineering in nonhuman primates.
Continuous O2-CO2 production using a Co-based oxygen carrier in two parallel fixed-bed reactors
Teng Zhang,Ningsheng Cai,Zhenshan Li 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3
Oxygen-enriched carbon dioxide stream with oxygen concentration higher than 20 vol% was produced continuously by using a Co-based oxygen carrier packed in two parallel fixed-bed reactors operated in a cyclic manner. Oxygen was absorbed by the oxygen carrier with air being fed. An oxygen-enriched carbon dioxide stream was obtained when the fixed-bed was regenerated with carbon dioxide as a purge gas. Multiple absorption and desorption cycles indicated that the Co-based oxygen carrier had high cyclic stability. XRD analysis determined the absorbed and desorbed products were Co3O4 and CoO, respectively. The TGA results indicated that Co-based oxygen carrier did not react with NO or SO2 during the desorption stage. This Co-based oxygen carrier offers potential for applications in the O2-CO2 production for the oxy-fuel coal combustion process.
Jun Li,Ningsheng Chen,Tao Wang,Javed Iqbal,Long Xiang 한국자원공학회 2017 Geosystem engineering Vol.20 No.4
A total volume of debris flow with intermittent surges is an important design parameter for the prevention of debris flow which requires an accurate determination of total volume of debris flow. Firstly, based on the data of 63 debris flow events from 1987 to 1995 at Jiangjia gully in Dongchuan County, Yunnan Province, China, we find that the distribution of debris flow discharge can be described by the lognormal distribution. Secondly, a statistical model for the total volume of debris flow with intermittent surges is established by the most stable fractile and the lognormal distribution function. Thirdly, we verified the accuracy of Equation (15) using data of 65 debris flows events from 1996 to 2004 in Jiangjia gully. Equation (15) generated more accurate results when compared with other existing models. The results of research are as follows: The Average Relative Error and Sum of Squared Relative Errors of Equation (15) are 47.6%p and 22.3, which is 10.9%p and 8.0 lower than that of Ou model, respectively. This model can be better applied to calculate the total volume of debris flows with intermittent surges in Jiangjia gully.
Coverless Multi-keywords Information Hiding Method Based on Text
Zhili Zhou,Yan Mu,Ching-Nung Yang,Ningsheng Zhao 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.9
As a new information hiding method, coverless information hiding has become a hot issue in the field of information security. The existing coverless information hiding method can hide only one Chinese character in each natural text. However, the problem of the method is that the hiding capacity is too small. To address this problem, a new method named coverless multi-keywords information hiding method based on text is proposed in this paper. The main idea of the method is that both the keywords and their number will be hidden in the texts. Experimental results show that the proposed method can improve the capacity of the existing coverless information hiding method based on text.
CO2 capture from flue gases using a fluidized bed reactor with limestone
Fan Fang,Zhen-shan Li,Ningsheng Cai 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.5
The CO2 capture from flue gases by a small fluidized bed reactor was experimentally investigated with limestone. The results showed that CO2 in flue gases could be captured by limestone with high efficiency, but the CO2 capture capacity of limestone decayed with the increasing of carbonation/calcination cycles. From a practical point of view, coal may be required to provide the heat for CaCO3 calcination, resulting in some potential effect on the sorbent capacity of CO2 capture. Experiment results indicated that the variation in the capacity of CO2 capture by using a limestone/coal ash mixture with a cyclic number was qualitatively similar to the variation of the capacity of CO2 capture using limestone only. Cyclic stability of limestone only undergoing the kinetically controlled stage in the carbonation process had negligible difference with that of the limestone undergoing both the kinetically controlled stage and the product layer diffusion controlled stage. Based on the experimental data, a model for the high-velocity fluidized bed carbonator that consists of a dense bed zone and a riser zone was developed. The model predicted that high CO2 capture efficiencies (>80%) were achievable for a range of reasonable operating conditions by the high-velocity fluidized bed carbonator in a continuous carbonation and calcination system.
Long Zhao,Yuxiang Chen,Yuanming Liu,Guofu Zhang,Juncong She,Shaozhi Deng,Ningsheng Xu,Jun Chen 한국물리학회 2017 Current Applied Physics Vol.17 No.1
Addressable field emitter arrays (FEAs) have important applications in vacuum electronic devices. However, it is important to integrate nanowire emitters into a gated structure without influencing the device structure and maintain the excellent field emission properties of nanowire emitters in the FEAs after the fabrication process. In this study, gate-structure ZnO nanowire FEAs were fabricated by a microfabrication process. The structure combines a planar gate and an under-gate, which is compatible with the preparation of ZnO nanowire emitters. The effect of electrode materials on the field emission properties of ZnO nanowires was studied using a diode structure, and it was found that ZnO nanowire pads on indium-tin-oxide (ITO) electrode showed better field emission performance compared with chromium (Cr) electrode. In addition, effective emission current modulation by the gate voltage was achieved and the addressing capability was demonstrated by integrating the ZnO nanowire FEAs in a vacuum-encapsulated field emission display. The reported technique could be a promising route to achieve large area addressable FEAs.