Effects of Copper and Selenium Supplementation on Performance and Lipid Metabolism in Confined Brangus Bulls

Netto, Arlindo Saran,Zanetti, Marcus Antonio,Claro, Gustavo Ribeiro Del,de Melo, Mariza Pires,Vilela, Flavio Garcia,Correa, Lisia Bertonha Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

Twenty-eight Brangus cattle were used to determine the effect of copper and selenium supplementation on performance, feed efficiency, composition of fatty acids in Longissimus dorsi (LD) muscle, and cholesterol concentration in serum and in LD muscle and enzymes activities, reduced glutathione (GSH) and oxidized glutathione (GSSG). The treatments were: i) Control, without copper (Cu) and selenium (Se) supplementation; ii) Se, 2 mg Se/kg of dry matter such as sodium selenite; iii) Cu, 40 mg Cu/kg of dry matter such as copper sulfate; iv) Se/Cu, 2 mg Se/kg of dry matter such as sodium selenite and 40 mg Cu/kg of dry matter such as copper sulfate. LD muscle fatty acid composition was not influenced by the treatments (p>0.05). The serum concentration of cholesterol was not influenced by the treatments (p>0.05), however, the concentration of cholesterol in LD was lower in cattle supplemented with copper and selenium (p<0.05). Oxidized glutathione and reduced glutathione increased (p<0.05) with Cu, Se and Se/Cu supplementation. The supplementation of copper (40 mg/kg DM) and selenium (2 mg/kg DM) altered the metabolism of lipids in confined Brangus cattle, through a decrease in cholesterol deposition in the LD, possibly by changing the ratio between reduced glutathione/oxidized glutathione. Copper and selenium supplementation improved animal performance and feed efficiency (p<0.05) when compared to the control group, providing advantages in the production system, while also benefiting consumers by reducing cholesterol concentration in the meat.

The Roles of Peroxiredoxin and Thioredoxin in Hydrogen Peroxide Sensing and in Signal Transduction

Netto, Luis E.S.,Antunes, Fernando Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.1

A challenge in the redox field is the elucidation of the molecular mechanisms, by which $H_2O_2$ mediates signal transduction in cells. This is relevant since redox pathways are disturbed in some pathologies. The transcription factor OxyR is the $H_2O_2$ sensor in bacteria, whereas Cys-based peroxidases are involved in the perception of this oxidant in eukaryotic cells. Three possible mechanisms may be involved in $H_2O_2$ signaling that are not mutually exclusive. In the simplest pathway, $H_2O_2$ signals through direct oxidation of the signaling protein, such as a phosphatase or a transcription factor. Although signaling proteins are frequently observed in the oxidized state in biological systems, in most cases their direct oxidation by $H_2O_2$ is too slow ($10^1M^{-1}s^{-1}$ range) to outcompete Cys-based peroxidases and glutathione. In some particular cellular compartments (such as vicinity of NADPH oxidases), it is possible that a signaling protein faces extremely high $H_2O_2$ concentrations, making the direct oxidation feasible. Alternatively, high $H_2O_2$ levels can hyperoxidize peroxiredoxins leading to local building up of $H_2O_2$ that then could oxidize a signaling protein (floodgate hypothesis). In a second model, $H_2O_2$ oxidizes Cys-based peroxidases that then through thiol-disulfide reshuffling would transmit the oxidized equivalents to the signaling protein. The third model of signaling is centered on the reducing substrate of Cys-based peroxidases that in most cases is thioredoxin. Is this model, peroxiredoxins would signal by modulating the thioredoxin redox status. More kinetic data is required to allow the identification of the complex network of thiol switches.

Effects of Dietary Selenium, Sulphur and Copper Levels on Selenium Concentration in the Serum and Liver of Lamb

Netto, Arlindo Saran,Zanetti, Marcus Antonio,Correa, Lisia Bertonha,Del Claro, Gustavo Ribeiro,Salles, Marcia Saladini Vieira,Vilela, Flavio Garcia Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.8

Thirty-two lambs were distributed in eight treatments under $2{\times}2{\times}2$ factorial experiment to compare the effects of two levels of selenium (0.2 to 5 mg/kg dry matter [DM]), sulphur (0.25% and 0.37%) and copper (8 and 25 mg/kg DM) levels on selenium concentration in liver and serum of lambs. A liver biopsy was done on all animals and blood samples were collected from the jugular vein prior to the beginning of the treatments. The blood was sampled every thirty days and the liver was sampled after 90 days, at the slaughter. Increasing differences were noticed during the data collection period for the serum selenium concentration, and it was found to be 0.667 mg/L in animals fed with 5 mg Se/kg DM and normal sulphur and copper concentrations in their diet. However, a three-way interaction and a reduction of selenium concentration to 0.483 mg/L was verified when increasing copper and sulphur concentration levels to 25 ppm and 0.37% respectively. The liver selenium concentration was also high for diets containing higher selenium concentrations, but the antagonist effect with the increased copper and sulphur levels remained, due to interactions between these minerals. Therefore, for regions where selenium is scarce, increasing its concentration in animal diets can be an interesting option. For regions with higher levels of selenium, the antagonistic effect of interaction between these three minerals should be used by increasing copper and sulphur dietary concentrations, thus preventing possible selenium poisoning.

Characterization of Photosynthetic Events and Associated Changes in Various Clones of Tea (Camellia sinensis L.) under Low Temperature Conditions

( L. A. Netto ),( K. M. Jayaram ),( P. Haridas ),( Jos T. Puthur ) 한국식물학회 2005 Journal of Plant Biology Vol.48 No.3

The Roles of Peroxiredoxin and Thioredoxin in Hydrogen Peroxide Sensing and in Signal Transduction

Luis E.S. Netto,Fernando Antunes 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.1

A challenge in the redox field is the elucidation of the molecular mechanisms, by which H2O2 mediates signal transduction in cells. This is relevant since redox pathways are disturbed in some pathologies. The transcription factor OxyR is the H2O2 sensor in bacteria, whereas Cys-based peroxidases are involved in the perception of this oxidant in eukaryotic cells. Three possible mechanisms may be involved in H2O2 signaling that are not mutually exclusive. In the simplest pathway, H2O2 signals through direct oxidation of the signaling protein, such as a phosphatase or a transcription factor. Although signaling proteins are frequently observed in the oxidized state in biological systems, in most cases their direct oxidation by H2O2 is too slow (101 M-1 s-1 range) to outcompete Cys-based peroxidases and glutathione. In some particular cellular compartments (such as vicinity of NADPH oxidases), it is possible that a signaling protein faces extremely high H2O2 concentrations, making the direct oxidation feasible. Alternatively, high H2O2 levels can hyperoxidize peroxiredoxins leading to local building up of H2O2 that then could oxidize a signaling protein (floodgate hypothesis). In a second model, H2O2 oxidizes Cys-based peroxidases that then through thiol-disulfide reshuffling would transmit the oxidized equivalents to the signaling protein. The third model of signaling is centered on the reducing substrate of Cys-based peroxidases that in most cases is thioredoxin. Is this model, peroxiredoxins would signal by modulating the thioredoxin redox status. More kinetic data is required to allow the identification of the complex network of thiol switches.

An Indian Family with Childhood Onset of Striatal Necrosis

Divya Nagabushana,Praveen-Kumar Srikanteswara,Archana Netto,Karthik Nagaraj 대한소아신경학회 2021 대한소아신경학회지 Vol.29 No.4

The Gene bap, Involved in Biofilm Production, Is Present in Staphylococcus spp. Strains from Nosocomial Infections

Amina Potter,Hilana Ceotto,Marcia Giambiagi-deMarval,Kátia Regina Netto dos Santos,Ingolf F. Nes,Maria do Carmo de Freire Bastos 한국미생물학회 2009 The journal of microbiology Vol.47 No.3

This study analyzed ten strains of coagulase-negative staphylococci (CNS) involved in nosocomial infections in three Brazilian hospitals. Their antibiotic susceptibility profile showed that most strains exhibited multiple antibiotic resistance and possessed the mecA gene. The ability of these strains to adhere to polystyrene microtiter plates was also tested and nine of them proved to be biofilm producers at least in one of the three conditions tested: growth in TSB, in TSB supplemented with NaCl, or in TSB supplemented with glucose. The presence of the bap gene, which codes for the biofilm-associated protein (Bap), was investigated in all ten strains by PCR. All strains were bap-positive and DNA sequencing experiments confirmed that the fragments amplified were indeed part of a bap gene. The presence of the icaA gene, one of the genes involved in polysaccharide intercellular adhesin (PIA) formation, was also detected by PCR in eight of the ten strains tested. The two icaA-negative strains were either weak biofilm producer or no biofilm producer, although they were bap-positive. To our knowledge, this is the first report demonstrating the presence of the bap gene in nosocomial isolates of CNS, being also the first report on the presence of this gene in Staphylococcus haemolyticus and S. cohnii.

처리(處理)를 달리한 소농업유역(小農業流域)의 유출(流出)과 토양유실(土壤流失)에 관(關)한 연구(硏究) -네브라스카주(州) 링컨지방(地方)을 중심(中心)으로-

이순혁 ( Soon Hyuk Lee ),( Howard D. Wittmuss ),( Jose Da Silva Madeira Netto ) 한국농공학회 1981 韓國農工學會誌 : 전원과 자원 Vol.23 No.1

本硏究는 3個의 서로 다른 農地保全處理를 가진 試驗區 및 테라스處理가 없는 牧草用 Bromegrass 試驗區에 있어서의 降雨, 流出現像과 土壤流失 關係를 究明함으로써 合理的인 農地保全 對策과 作付體系를 提示코저 하는데 그 目的이 있다. 降雨, 流出 및 土壤流失에 關한 分析考察은 3個의 農地保全 試驗區에서는 5年間(1972-1976年)의 資料를 그리고 Bromegrass 牧草試驗區에서는 3年間(1972-1974年)의 資料를 對象으로 하였다. 3個의 農地保全處理區는 첫째로Non-terrace에 秋耕 春條播形式의 慣行法에 의한 非處理區와 둘째로 急逆勾配 테라스를 가진 草生水路에 가을 收穫時 crop residue를 남겨두고 봄에 耕作條播하는 Till plant system을 가진 草生水路區 그리고 마지막으로 地下排水와 함께 逆勾配 Terrace를 具備한 條播의 Till plant system 을 採用한 排水區를 나누어진다. Bromegrass 處理區는 Terrace나 其他 農地保全策이 없는 牧草用 試驗區 이다. 以上의 硏究에서 얻어진 結果를 要約하면 다음과 같다. 1. 試驗期間內의 平均降水量은 네브라스카 링컨 地方의 76年間의 平均値, 728mm보다 66mm가 적은 662mm이였고 1974年과 1975年은 平均値 보다 300mm나 적은 極히 乾燥한 해 이였으며 1972年과 1976年은 거의 平均降水量에 가까웠고 1973年만은 平均値보다 240mm나 더 많은 多雨年 이었다. 2. 慣行의 作付體系를 가진 非處理區에서의 年平均 流出高(2.44cm)는 逆勾配의 Terrace를 가지고 Till-plant system을 採擇한 草生水路區의 流出高(2.42cm)와 거의 類似한 値였다. 3. 排水處理區로부터의 流出은 3個의 農地保全 處理區 中에서 5年間의 平均値가 1.57cm/year로 草生水路區와 非處理區의 流出보다 35%나 적은 가장 낮은 結果를 나타내었다. 4. Bromegrass 牧草處理區에서의 流出은 放牧程度에 따라서 影響되었다. 1972年의 無放畜時 流出은 다른 3個 處理區로 부터의 流出의 7 乃至 19%內인 0.07cm 程度였으나 1973年과 1974年의 放畜年에는 條播 3個 處理區(3.4~5.18cm)에 필적되는 年平均 4.16cm로 나타났다. 5. 全體 試驗區로부터의 年平均 土壤流失量은 Sharpsburg 粘質壤土에 對한 S.C.S 限界許容値 10ton/ha/year 以內로 나타났다. 非處理區에서의 土壤流失量은 平均 2.56ton/ha/year로 높게 나타난 反面 3個의 서로 다른 處理區인 排水區, 草生水路區 및 Bromegrass區에서는 各各 0.152, 0.192 및 0.290ton/ha/year로 낮은 結果를 가져왔다. 6. 平均 沈澱量에 對한 L.S.D. 檢定 結果 全試驗區中 非處理區가 高度의 有意差를 나타낸 反面 排水區, 草生水路區 및 Bromegrass 牧草區 間에는 아무런 有意差가 認定되지 않았다. 7. 農地保全 處理區인 排水區와 草生水路區는 非處理區에 比해 낮은 尖頭 流出量과 낮은 土壤流失量을 나타내었다.

Evaluation of the Hypotensive Potential of Bovine and Porcine Collagen Hydrolysates

Mariza Faria,Elizabete Lourenço da Costa,José Antônio Rocha Gontijo,Flávia Maria Netto 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.3

Angiotensin-converting enzyme (ACE) inhibitory activity and antihypertensive activity of bovine and porcine collagen hydrolysates in spontaneously hypertensive rats (SHR) were investigated. The hydrolyzed collagens were subjected to ultrafiltration using membranes with cutoffs of 30–50 kDa (permeate P1), 5–8 kDa (permeate P2), or 1–2 kDa (permeate P3) in order to obtain products with a narrower range of molecular size. The hydrolyzed bovine and porcine collagens and their permeates showed low ACE inhibitory activity (50% inhibitory concentration [IC50] = 5.42–15.58 mg of protein/mL). However, after in vitro gastrointestinal digestion, a significant increase in the ACE inhibitory potency of the hydrolyzed collagens was observed (IC50 = 0.97–4.02 mg of protein/mL). Permeates had a higher ACE inhibitory activity and hypotensive activity than non-ultrafiltered hydrolysates. The P1 permeate of bovine and porcine collagen and the P3 fraction of the porcine collagen hydrolysate exhibited the best antihypertensive activity in vivo, promoting a maximum reduction in blood pressure of 22 mm Hg, 21.33 mm Hg, and 21.33 mm Hg, respectively, while lisinopril promoted a maximum reduction of 51.00 mm Hg. These results suggest that the commercial collagen hydrolysates of bovine and porcine origin may be a potential source of bioactive peptides.

Lymph node density predicts recurrence and death after inguinal lymph node dissection for penile cancer

Mark W. Ball,Zeyad R. Schwen,Joan S. Ko,Alexa Meyer,George J. Netto,Arthur L Burnett,Trinity J. Bivalacqua 대한비뇨의학회 2017 Investigative and Clinical Urology Vol.58 No.1

Purpose: To determine the impact of lymph node density (LND) on survival after inguinal lymph node dissection (ILND) for penile cancer. Materials and Methods: Our institutional penile cancer database was queried for patients who underwent ILND. Clinicopathologic characteristics including LND and total number of positive lymph nodes (LNs) were analyzed to determine impact on recurrence-free survival (RFS) and overall survival (OS). LND, or the percent of positive LN out of total LN, was calculated as a categorical variable at varying thresholds. Results: Twenty-eight patients with complete follow-up were identified. Indications for ILND were stage >T2 in 20 patients (71.4%), palpable adenopathy in 7 (25%), high grade T1 in 1 (3.6%). Median node yield was 17.5 (interquartile range, 12−22), and positive LNs were found in 14 patients (50%). RFS and OS were significantly lower for patients with >15% LN density (median RFS: 62 months vs. 6.3 months, p=0.0120; median OS: 73.6 months vs. 6.3 months, p<0.001). Controlling for age, medical comorbidities, number of positive LN, T stage, pelvic LN status and indication, LN density >15% was independently associated with worse RFS (hazard ratio [HR], 3.6; p=0.04) and OS (HR, 73.6; p=0.002). The c-index for LND was higher than total positive LNs for RFS (0.64 vs. 0.54) and OS (0.79 vs. 0.61). Conclusions: In this small, retrospective penile cancer cohort, the presence of nodal involvement >15% was associated with decreased RFS and OS, and outperformed total number of positive LN as a prognostic indicator.