Identification of cytokines inhibiting eosinophil activation and the inhibitory mechanism

Park,,Choon-Sik,Choi,,Eun,Nam,Lee,,Young,Mok,Park,,Sung,Woo,Jang,,An,Soo,Lee,,June,Hyuk,Choi,,Yun,Sung,Chung,,Il,Yup 대한천식 및 알레르기학회 2003 천식 및 알레르기 Vol.23 No.4

IL-5는 여러 경로를 통하여 호산구의 기능에 결정적인 역할을 하고 혈액이나 조직에서 호산구의 수적인 증가를 고려할 때 호산구 증다증을 동반하는 여러 질환과 깊게 연관되어 있다. 본 실험에서 IL-5로 자극된 호산구와 자극되지 않은 호산구의 활성을 pro-inflammatory cytokines(IL-1β , IL-6, TNF-α )과 Th1(IFN- γ), Th2(IL-4, IL-13) 그리고 Tr cytokines(IL-10)이 억제할 수 있는지를 eosinophil shape change를 통해서 알아보았다. 시간과 투여량에 따라 IL-6와 TNF- 는 자극되지 않은 호산구의 크기를 증가시키는 반면, IFN- γ와 IL-4 그리고 IL-13은 호산구의 크기를 유의하게 감소시켰다. 또한 IL-13을 제외한 IFN- γ와 IL-4는 IL-5로 자극된 호산구의 shapec hange를 억제했다. 최대 억제를 위해서 IFN- γ와 IL-4로 30~60분 정도 전처리 하였다. cyclohexamide에 의한 억제 효과의 제거는 cytokine에 의한 신호전달과 IFN- γ의 효과에 있어서 새로운 단백질이 필요함을 제시하였다. IFN- γ의 전처치는 IL-5에 의하여 유도된 ERK1/2의 인산화를 유의하게 억제하였다. 결론적으로 IFN- γ는 새로운 단백질의 생성으로 MAP kinase 활성을 억제하여 IL-5로 자극된 호산구의 기능을 조절할 수 있다. (J Asthma Allergy Clin Immunol 23: 759-72, 2003)

Interleukin-1B(1L-1B) polymorphisms and gastric mucosal levels of IL-Iβ cytokine in Korean patients with gastric cancer

Chang,,Young-Woon,Jang,,Jae-Young,Kim,,Nam-Hoon,Lee,,Jae,Won,Lee,,Hyo,Jung,Jung,,Woon,Won,Dong,,Seok-Ho,Kim,,Hyo-Jong,Kim,,Byung-Ho,Lee,,Joung-Il,Rin,Chang KYUNG HEE UNIVERSITY MEDICAL CENTER 2006 고황의학상 수상논문집 Vol.21-22 No.-

Interleukin-1B and IL-1 receptor antagonist gene polymorphisms are associated with an increased risk of gastric cancer (GC) in Caucasian populations. However, recent studies could not find any association between IL-1B-511T polymorphism and the risk of GC in Asians. We tested for an association between IL-1 loci polymorphisms with increased gastric mucosal levels of IL-1β and an increased risk of developing GC in a Korean population. Polymorphisms of IL-1A-889, IL-1B-31, IL-1B-511 and IL-1RN were genotyped in 434 controls and 234 patients with GC. Mucosal IL-1β cytokine was measured using an ELISA. The frequencies of IL-1A, IL-1B-511, IL-1B-31 and IL-1RN were not statistically different between controls and all patients with GC. After subclassification of GC, only patients with intestinal-type GC showed a higher frequency of IL-1B-31T homozygotes (OR = 2.2; 95% CI = 1.1-4.3) compared with controls. Risk was also significantly increased in these patients for IL-1B-31T homozygotes compared with patients with diffuse-type GC (OR = 3.4; 95% CI = 1.5-7.7). As in Caucasian populations, linkage disequilibrium between IL-1B-31 and IL-1B-511 was nearly complete, but the pattern of haplotype related to the risk of GC (IL-1B-31T/IL-1B-511C) was opposite (IL-1B-511T/IL-1B-31C). Mucosal IL-1β levels in H. pylori-infected GC patients were higher in patients homozygous for IL-1B-31T compared with IL-1B-31C/T and IL-1B-31C/C. Thus, the combined effects of H. pylori infection and IL-1B-31T/IL-1B-511C polymorphisms with enhanced mucosal IL-1β production contributed to the development of intestinal-type GC in this Korean population.

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Il-seob,Shin,Hye-ryon,Kim,Myung-jin,Nam1,and,Hwa-young,Youn 대한수의학회 2001 JOURNAL OF VETERINARY SCIENCE Vol.2 No.2

'스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.

Equimolar Carbon Dioxide Absorption by Ether Functionalized Imidazolium Ionic Liquids

Pankaj,Sharma,Sang,Do,Park,Ki,Tae,Park,정순관,Sung,Chan,Nam,Il,Hyun,Baek 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

A series [C3Omim][X] of imidazolium cation-based ILs, with ether functional group on the alkyl side-chain have been synthesized and structure of the materials were confirmed by various techniques like 1H, 13C NMR spectroscopy, MS-ESI, FTIR spectroscopy and EA. More specifically, the influence of changing the anion with same cation is carried out. The absorption capacity of CO2 for ILs were evaluated at 30 and 50 ºC at ambient pressure (0-1.6 bar). Ether functionalized ILs shows significantly high absorption capacity for CO2. In general, the CO2 absorption capacity of ILs increased with a rise in pressure and decreased when temperature was raised. The obtained results showed that absorption capacity reached about 0.9 mol CO2 per mol of IL at 30 ºC. The most probable mechanism of interaction of CO2 with ILs were investigated using FTIR spectroscopy, 13C NMR spectroscopy and result shows that the absorption of CO2 in ether functionalized ILs is a chemical process. The CO2 absorption results and detailed study indicates the predominance of 1:1 mechanism, where the CO2 reacts with one IL to form a carbamic acid. The CO2 absorption capacity of ILs for different anions follows the trend: BF4 < DCA < PF6 < TfO < Tf2N. Moreover, the as-synthesized ILs is selective, thermally stable, long life operational and can be recycled at a temperature of 70 ºC or under vacuum and can be used repeatedly.

Equimolar Carbon Dioxide Absorption by Ether Functionalized Imidazolium Ionic Liquids

Sharma,,Pankaj,Park,,Sang-Do,Park,,Ki-Tae,Jeong,,Soon-Kwan,Nam,,Sung-Chan,Baek,,Il-Hyun Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

A series $[C_3Omim]$[X] of imidazolium cation-based ILs, with ether functional group on the alkyl side-chain have been synthesized and structure of the materials were confirmed by various techniques like $^1H$, $^{13}C$ NMR spectroscopy, MS-ESI, FTIR spectroscopy and EA. More specifically, the influence of changing the anion with same cation is carried out. The absorption capacity of $CO_2$ for ILs were evaluated at 30 and $50^{\circ}C$ at ambient pressure (0-1.6 bar). Ether functionalized ILs shows significantly high absorption capacity for $CO_2$. In general, the $CO_2$ absorption capacity of ILs increased with a rise in pressure and decreased when temperature was raised. The obtained results showed that absorption capacity reached about 0.9 mol $CO_2$ per mol of IL at $30^{\circ}C$. The most probable mechanism of interaction of $CO_2$ with ILs were investigated using FTIR spectroscopy, $^{13}C$ NMR spectroscopy and result shows that the absorption of $CO_2$ in ether functionalized ILs is a chemical process. The $CO_2$ absorption results and detailed study indicates the predominance of 1:1 mechanism, where the $CO_2$ reacts with one IL to form a carbamic acid. The $CO_2$ absorption capacity of ILs for different anions follows the trend: $BF_4$ < DCA < $PF_6$ < TfO < $Tf_2N$. Moreover, the as-synthesized ILs is selective, thermally stable, long life operational and can be recycled at a temperature of $70^{\circ}C$ or under vacuum and can be used repeatedly.

Interleukin-17이 배양된 류마티스관절염 활막세포에서 vascular endothelial growth factor 생성에 미치는 영향

곽임수 ( Ihm Soo Kwak ), 남태수 ( Tae Soo Nam ), 나하연 ( Ha Yeon Rha ), 서정탁 ( Jeung Tak Suh ), 김유선 ( Yoo Sun Kim ), 김성일 ( Sung Il Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To investigate the the effects of interleukin-17 (IL-17) on the production of vascular endothelial growth factor (VEGF) from cultured rheumatoid arthritis synoviocytes. Methods: Fibroblast-like synovial cells(FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-β(TGF-β), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). VEGF levels were determined in the culture supernatants by sandwitch ELISA. Results: Stimulation of FLS by serial concentration of IL-17, TGF-β, TNF-α, IL-1β increased the production of VEGF by 2.1-2.7, 2.2-3.0, 2.0-2.9, 2.3-3.1 fold over the constitutive levels of unstimulated FLS. Stimulation of FLS by IL-17 with TGF-β or TNF-α or IL-1β also increased the production of VEGF according to culture periods by 1.6-1.8, 1.1-1.9, 1.5-1.7 fold over the levels stimulated with TGF-β or TNF-α or IL-1β, respectively. This results indicated that IL-17 increased the effect of TGF-β, TNF-α, IL-1β on FLS, leading synergistic enhancement of VEGF production. Conclusion: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.

Nam's Method에 의한 下顎顆頭 및 頸部 骨折 處置 (II)

Il Woo Nam(南日祐) 대한구강악안면외과학회 1980 대한구강악안면외과학회지 Vol.6 No.1

'스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

It is very difficult that the fractured condylar head and upper condylar neck have to be reduced and immobilized properly. The reduction and fixaton of the fractured those bones are so difficult to be reduced and fixated that many oral surgeons are trying to study on how to operate an adequate reduction and fixation. In spite of endeavor, lots of fragments of the fractured condylar heads frequently have been removed as so-called condylectomies because complexities of the surrounding anatomical structures make adequate reductions and fixations impossible. Thus the author has developed a new method named Dr. Nam s method in terms of oblique osteotomy, interosseous wiring extraorally, and replantation by which I have successfully treated 48 patients with fractures of the condylar head or upper part of the condylar neck of the mandibles since 1976. In order to reduce and immobilize those fractured, firstly, oblique osteotomy at the affected ascending ramus of the mandible was preceded, and secondly, the separated posterior part of the ascendingramus and fractured condylar head were carefully removed out from the body to be reduced and immobilized together extraorally, and lastly, replantations were made at the same sites. The results were drawn as follows: 1. The author successfully has treated 48 patients with the condylar head or upper condylar neck fractures by using Dr. Nam s method. 2. No intermaxillary fixation or less than 1 week intermaxillary wiring was needed after surgery. 3. The normal mouth opening and closing, and facial appearnaces could be observed in 1 month aft er surgery. 4. Traumatic injury for instance, automobile accident, was one of the commonest causes of fractures of the condylar head and upper condylar neck. 5. The features of those fractures were type I; one single fragment of upper condylar neck, Type II ; oblique. single fracture from the inner condylar head to its articular surface, and type III ; fractures with 3 pieces condylar head in the order.

Association of DOCK8, IL17RA, and KLK12 Polymorphisms with Atopic Dermatitis in Koreans

( Won Il Heo ), ( Kui Young Park ), ( Mi-kyung Lee ), ( Yu Jeong Bae ), ( Nam Ju Moon ), ( Seong Jun Seo ) 대한피부과학회 2020 Annals of Dermatology Vol.32 No.3

Background: Early-onset and severe atopic dermatitis (AD) in patients increase the probability of the development of allergic rhinitis or asthma. Treatment and prevention strategies in infants and young children with AD are targeted toward treating the symptoms, restoring skin barrier functions, and reducing the absorption of environmental allergens in an attempt to attenuate or block the onset of asthma and food allergy. Objective: Given that the initiating events in AD remain poorly understood, identifying those at risk and implementing strategies to prevent AD is necessary. Methods: Whole-exome sequencing (WES) was performed in a 43 control group and a disease group with 20 AD patients without atopic march (AM) and 20 with AM. Sanger sequencing was carried out to validate found variants in cohorts. Results: DOCK8, IL17RA, and KLK12 single-nucleotide polymorphisms were identified by WES as missense mutations: c.1289C>A, p.P97T (rs529208); c.1685C>A, p.P562G (rs12484684); and c.457+27>C, rs3745540, respectively. A case-control study show that total immunoglobulin E (IgE) level was significantly increased in the AA genotype of DOCK8 compared to the CA genotype in allergic patients. The rs12484684 of IL17RA increased risk of adult-onset AD (odds ratio: 1.63) compared to the control for (A) allele frequency. AD and AM Patients with the IL17RA CA genotype also had elevated IgE levels. rs3745540 of KLK12 was associated with AD in dominant model (odds ratio: 2.86). Conclusion: DOCK8 (rs529208), IL17RA (rs12484684), and KLK12 (rs3745540), were identified using a new WES filtering method. the result suggests that polymorphism of DOCK8 and IL17RA might be related to increase the total IgE level. (Ann Dermatol 32(3) 197∼205, 2020)

Original Article : The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

( Su Youn Nam ), ( Il Ju Choi ), ( Kum Hei Ryu ), ( Bum Joon Park ), ( Young Woo Kim ), ( Hyun Beom Kim ), ( Jeongseon Kim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims Although adipocytes secrete inflammatory cytokines and adipokines, their role in reflux esophagitis is controversial. We investigated the association between visceral fat and inflammatory cytokines or adipokines in reflux esophagitis. Methods Abdominal visceral fat and cytokines were measured in 66 individuals with reflux esophagitis and 66 age- and sex-matched controls. The mean values for visceral fat and cytokines were compared in cases and controls. Second, correlations between visceral fat and inflammatory cytokines were measured. Finally, multiple logistic regression models for odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the effects of visceral fat and cytokines on reflux esophagitis. Results Visceral fat, leptin, interleukin (IL)-6, and IL-1β were higher in reflux esophagitis compared to controls. Visceral fat showed a strong positive correlation with IL-6 (r = 0.523, P < 0.001), IL-8 (r = 0.395, P < 0.001), and IL-1β (r = 0.557, P < 0.001), and a negative correlation with adiponectin (r = -0.466, P < 0.001). With adjusted analysis, visceral fat/100 (OR, 4.32; 95% CI, 2.18-8.58; P < 0.001) and leptin (OR, 1.36; 95% CI, 1.10-1.69; P = 0.005) independently increased the risk of reflux esophagitis, but the effects of other cytokines were abolished. Conclusions Visceral fat may increase the risk of reflux esophagitis by increasing the levels of inflammatory cytokines. Leptin showed a positive association with reflux esophagitis that was independent of visceral fat. (J Neurogastroenterol Motil 2015;21:247-254)

Recovery of ionic liquid and sugars from hydrolyzed biomass using ion exclusion simulated moving bed chromatography

Mai,,Ngoc,Lan,Nguyen,,Nam,Trung,Kim,,Jin-Il,Park,,Hyuk-Min,Lee,,Sung-Kyun,Koo,,Yoon-Mo Elsevier 2012 Journal of chromatography Vol.1227 No.-

<P><B>Highlights</B></P><P>► Ionic liquid was successfully separated from aqueous sugar mixtures by ion exclusion SMB. ► Ionic liquid and sugar recovery yield depend on the SMB zone flow rates. ► Complete recovery of ionic liquid could be obtained by optimization of SMB zone configuration.</P> <P><B>Abstract</B></P><P>Efficient recovery of ionic liquid (IL) from aqueous mixture of ILs and sugars (which derived from enzymatic or chemical catalyzed hydrolysis of ILs-pretreated biomass) is a major drawback for commercialization of biofuel and platform chemicals production from biomass utilized ILs as pretreatment solvent. In this study, simulated moving bed (SMB) chromatography equipped with ion exclusion column (containing [Emim]<SUP>+</SUP> cation) was investigated to separate sugars (glucose and xylose) which are the main products from biomass hydrolysate and 1-Ethyl-3-methylimidazolium acetate (EmimAc) which is the ILs used for biomass pretreatment. A four-zone SMB system with a configuration of 2-2-2-2 (2 ion exclusion columns in each zone) was used to recover glucose, xylose and EmimAc from their aqueous mixture with yield of 71.38, 99.37 and 98.92%, respectively. Moreover, the optimization of SMB zone configuration by simulation results in a complete recovery of ILs. This result indicates that for the first time, ion exclusion SMB chromatography could be used for complete recovery of ILs from aqueous sugar mixture.</P>