Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

원칩마이크로컨트롤러를 이용한 단상유도전동기의 기동시스템 구현

백형래,박수강,최낙일 조선대학교 에너지.자원신기술연구소 1998 에너지·자원신기술연구소 논문지 Vol.20 No.2

A single phase induction motor(SPIM) with two windings, main and auxiliary is probably the most widely used motor in the world due to their ruggedness, low maintenance and simplicity of construction. There are several ways of starting single phase motors. The most popular arrangements is centrifugal switch. An auxiliary winding connected in series with a capacitor, is brought into the circuit during starting. The closing and opening of the switch must be speed dependant. The switch meets the speed requirements, but it also has many drawback: random switching, arcing, many models necessary for different speeds and shaft diameters, space required inside the motors and limited lifetime to name some of them. In this paper, authors present the experimental results of the SPIM under controlling of currents of main and auxiliary windings by using a one-chip micro starter. This new starting method is developed to replace the conventional starting device consisted with a mechanical switch and a capacitor. In mentioned method the soft-starting of SPIM is simply implemented and higher efficiency is obtained in comparison with conventional condenser type SPIM.

광섬유 ROTDR 센서를 이용한 사회기반시설물의 보안에 관한 연구

박형준,고광락,권일범 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.2

광섬유 ROTDR(Rayleigh optical time domain reflectometry) 센서를 이용하여 중요 보안 대상체인 사회기반시설물에 침투하는 침입자의 위치와 무게 정도를 탐지할 수 있는 기초 연구를 수행하였다. 넓은 면적을 감지할 수 있는 매설형 광섬유 센서탐지판을 제작하고, 인가된 침입물체의 위치와 무게에 따른 신호특성을 고찰하였다. ROTDR 센서는 펄스 폭이 30 ㎱이고, 광섬유의 길이는 10㎞ 이상이다. 위치탐지오차는 약 3m 이내였으며, 무게에 따른 탐지능력은 20kgf, 40kgf, 60kgf의 세단계를 구분할 수 있음을 알 수 있었다. A detection technique is studied to determine the location and the weight of an intruder into infrasturcture using fiber optic ROTDR(Rayleigh optical time domain reflectometry) sensor. Fiber optic sensing plates buried in sand are prepared to measure the intruder effects. The signal of ROTDR was analyzed to confirm the detection performance. The constructed ROTDR system could be used up to 12 ㎞ at the pulse width of 30 ㎱. The location error was less than 3 m and the weight could be detected into three levels of grade, such as 20 kgf, 40 kgf and 60 kgf.

필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

머리염색이 인체에 미치는 영향

윤형식,황성호,이현륭,김수호,박연석,권낙현,정호진,김동훈,노현주,홍성호,박병찬,이관,정해관 東國大學校醫學硏究所 2002 東國醫學 Vol.9 No.1

일상생활에서 모발염색은 흔히 접할 수 있는 미용의 한 종류로 특히 젊은층을 중심으로 폭발적으로 유행하고 있다. 염색을 위항 사용하는 약제는 표백제와 발색제 등 각종 화학약품이 사용되고 있으나 이로 인한 건강장해에 대한 연구는 그리 많지 않다. 저자들은 염색이 인체의 모발건강에 미치는 영향을 파악하기 위하여 염색과 관련된 주관적 증상과 모발의 변화에 대한 실험적 연구를 시행하였다. 동국대학교 경주 캠퍼스 재학생 80명을 대상으로 설문조사를 시행하여 염색 유 ·무 및 염색 후에 경험한 증상에 대해 설문 조사하였고, 의과대학 재학생 46명을 대상으로 피부 반응 테스트를 실시하였다. 또한 염색 전후의 모발 탄성도를 측정하였고 모발의 상태를 파악하기 위해 전자 현미경검사를 실시하였다. 설문조사 결과 염색 전에 비하여 염색 후 안구혼탁, 안구건조, 시력저하, 발진 및 접촉성 피부염, 모발손상, 모근손상 등의 증상을 더 많이 경험한다고 호소하였다(p<0.05). 모발손상과 모근손상은 헤어드라이어 사용 빈도에 따라 증가하는 것으로 조사되었다(p<0.05). 피부반응검사에서 가려움증이 가장 많은 증상이었으며 이는 여성보다는 남성에서 높은 것으로 조사되었다. 염색 전후의 모발장력은 염색 전 134.5±10.37g, 염색 128.0±30.69g, 염색 이틀 후 112.5±19.69g으로 나타났다. 염색 전후의 모발의 전자현미경 케라틴 층이 현저히 감소하고 모발이 가늘어지는 차이를 보였다. 염색은 모발손상, 모발 케라틴 손상 및 모근 손상, 발진 및 접촉성 피부반응, 안구혼탁, 안구건조, 시력 저하를 유발한다. 따라서 염색약으로 인한 손상에 대한 주의와 예방이 필요하다고 생각한다. 예방대책으로 염색 전 피부테스트를 통한 적합성 여부를 판단하는 것이 필요하며 가급적 염색을 피하는 것이 좋을 것이다. 염색약에 발암물질이 포함되어있다는 보고도 있어 염색 제조사의 철저한 실험과 염색 물질의 선별이 염색으로 인한 부작용을 최소화하는데 중요한 역할을 할 것이다. Hair coloring has became one of the most popular cosmetic activities to younger generations during last decade. However, there are few studies on the health effect of widespread use of chemical dyes. This study was conducted to study the effects of hair coloring dye on hair and other systems. We conducted a questionnaire survey of 80 persons in Kyongju campus, Dongguk University. We have done open patch skin test on 46 medical students. We also conducted scanning electron microscopy to examine the hair strength and structure before and after hair coloring process. Injury of hair and hair bulb, contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity were the main symptoms complained after hair coloring (p<0.05). Injury of hair and hair bulb were increased by frequency of hair-dryer use(p<0.05). In open patch test, pruritus was complanined by more than half of the subjects. Mean strength of hairs before and after hair coloring was as follows; 134.5 (SD 10.37)g before hair coloring, 128.0 (SD 30.69)g immediately after hair coloring, and 112.5 (SD 19.69)g after two days. The scanning electron microscopic findings of hair surface before and after hair coloring showed decreased keratin layer and thinning of the hair. Hair coloring induces injury to hair, its keratin layer, and hair bulb as well as contact dermatitis, turbid eyes, xerophthalmia, and poor visual acuity. Therefore, we think that precaution is needed in use of hair coloring dye. To prevent complications induced by hair coloring dye, it is necessary, especially to those with allergy or skin disorders, to perform skin test before action and avoid hair coloring whenever possible. Longterm health effects of hairdye should be studied and manufacturing companies should try to minimize complications induced by hair coloring dye.

하수슬러지 소화조내 음식물쓰레기 투입시 고형물분해에 관한 연구

이영형,성낙창,박출재 동아대학교 환경문제연구소 1998 硏究報告 Vol.21 No.2

This study aims to find a solution to the problem how to treat increasing food waste as time goes on. And this study develops the digesting method of mixing food waste and sewerage sludge as a part of treating food waste. sewerage sludge and food waste were digested in mesophilic condition with the mixing ratio of 1 to 0.1, 1 to 0.3, 1 to 0.5, 1 to 1 and 1 to 2 respectively for 40 days. As the result, the digester(tank) with mixture packing of food waste showed stable in about 11days of digestion period, and in particular, in case of 1 to 0.1 as mixing proportion, removal of VS was the most enormous. The highest chlorine ionic concentration in digestion showed 4700mg/l, which didn't have great effects on removal of VS.

전류제어에 의한 단상유도전동기의 기동특성 개선

박수강,최낙일,백형래,임양수,이기용,신사현 조선대학교 에너지.자원신기술연구소 1998 에너지·자원신기술연구소 논문지 Vol.20 No.1

A single phase induction motor(SPIM) with two windings, main and auxiliary, is probably the most widely used motor in the world due to their ruggedness, low maintenance and simplicity of construction There are several ways of starting single phase motors The most popular arrangements is centrifugal switch An auxiliary winding connected in series with a capacitor, is brought into the circuit during starting The closing and opening of the switch must be speed dependant The switch meets the speed requirements, but it also has many drawback random switching, arcing, many models necessary for different speeds and shaft diameters, space required inside the motors and limited lifetime to name some of them. In this paper, the starting current of single phase induction motor was detected by a small detector and determined TRIAC gate signal. The strategy of current control is based on limited main and auxiliary current angle during starting period in the closed-loop control. The experimental result show the motor's starting characteristics of the proposal starting system and are compared with centrifugal switch starting motor.

Mycobacterium mageritense에 의한 당뇨병성 족부 감염증 1예

김충종,김낙현,김문석,김계형,전재현,박문석,박경운,박완범,박상원,김홍빈,김남중,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Diabetic foot infection is one of the important complications in patients with advanced diabetes mellitus. Limb threatening infections such as osteomyelitis, abscess, and necrotizing fasciitis are frequently accompanied by the disease. Non-tuberculous mycobacterium (NTM) is a rare causative organism of diabetic foot infection. Thus, if one is not suspicious or meticulous, infection due to NTM will be easily overlooked and this will result in delayed diagnose and treat. Therefore, it is necessary to consider NTM as the causative organism if the wound does not respond to the conventional antibiotic treatment and the culture from the adequately obtained specimen reveals atypical acid-fast bacilli. We present a case of diabetic foot infection with osteomyelitis and abscess due to Mycobacterium mageritense, one of the rapid growing mycobacteria, that was successfully treated with surgical debridement and appropriate antibiotic treatment.

선박의장 배치 설계의 검도를 위한 컴퓨터 지원 시스템의 개발 사례

노재정,조형석,전진영,성낙운 경성대학교 공학기술연구소 1999 공학기술연구지 Vol.6 No.-

The purpose of this study is to develop a computer support system for the inspection of the outfitting arrangement of a ship design, which will increase the accuracy and the effectiveness of the inspection process. This system is integrated with a ORACLE database for transferring and storing 3D CAD data into the inspection system, and a dVISE virtual reality system for displaying the attributes of entities and the inspection results.

The Structure of the Nucleons as Compound of a Bradyon and a Tachyonic Pion

현남규,홍낙형 濟州大學校 師範大學 科學敎育硏究所 1995 科學敎育 Vol.12 No.1