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Naheed Akhtar,Vinitha Moolchand Thadhani,Faraz Ul Haq,Muhammad Noman Khan,Sajjad Ali,Syed Ghulam Musharraf 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.90 No.-
Tea beverages have been enjoyed globally for the last several decades. The present study focuses on the comprehensive chemical and biological analysis of three processed tea products. A total of sixty-three compounds in processed samples were identified based on their exact masses and fragmentation patterns using LC-ESI-QTOF-MS/MS. Furthermore, quantification of eight analytes including caffeine (1), theophylline (2), (+)-catechin (3), (-)-epicatechin (4), (-)-epicatechin gallate (5), (-)-gallocatechin (6), (-)-epigallocatechin gallate (7) and quercetin-3-d-β-glucoside (8) in tea samples was performed using LC-ESI-IT-MS/MS. The concentrations of analytes were found in the range of 0.03 mg/g to 75.58 mg/g in all tea samples. The developed method showed excellent accuracy as %bias ranged from 0.2–3.69% and a good precision with %RSD ranged from 0.03 to 5.11. The LOD and LOQ for all the analytes were found to be in the range of 0.16–3.26 ng/mL and 0.44–9.87 ng/mL, respectively. The DPPH scavenging effects of samples were also investigated and all the samples were found to be strong scavengers of DPPH radical showing 69.50 ± 0.01–78.60 ± 0.10 %RSA. The established method provides a useful way for understanding the metabolite distribution in processed products of C. sinensis and to develop quality control protocols for these products.
Qaisra Naheed Choudhry,김준호,조형택,Wan Heo,이정준,이진협,김영준 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.2
Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steamprocessed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG). Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with H2O2, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated. Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging. Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stressinduced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system.
Choudhry, Qaisra Naheed,Kim, Jun Ho,Cho, Hyung Taek,Heo, Wan,Lee, Jeong-Jun,Lee, Jin Hyup,Kim, Young Jun The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.2
Background: Oxidative stress induces the production of reactive oxygen species (ROS), which play important causative roles in various pathological conditions. Black ginseng (BG), a type of steam-processed ginseng, has drawn significant attention due to its biological activity, and is more potent than white ginseng (WG) or red ginseng (RG). Methods: We evaluated the protective effects of BG extract (BGE) against oxidative stress-induced cellular damage, in comparison with WG extract (WGE) and RG extract (RGE) in a cell culture model. Ethanolic extracts of WG, RG, and BG were used to evaluate ginsenoside profiles, total polyphenols, flavonoid contents, and antioxidant activity. Using AML-12 cells treated with $H_2O_2$, the protective effects of WGE, RGE, and BGE on cellular redox status, DNA, protein, lipid damage, and apoptosis levels were investigated. Results: BGE exhibited significantly enhanced antioxidant potential, as well as total flavonoid and polyphenol contents. ATP levels were significantly higher in BGE-treated cells than in control; ROS generation and glutathione disulfide levels were lower but glutathione (GSH) and NADPH levels were higher in BGE-treated cells than in other groups. Pretreatment with BGE inhibited apoptosis and therefore protected cells from oxidative stress-induced cellular damage, probably through ROS scavenging. Conclusion: Collectively, our results demonstrate that BGE protects AML-12 cells from oxidative stress-induced cellular damages more effectively than WGE or RGE, through ROS scavenging, maintenance of redox status, and activation of the antioxidant defense system.
Haseen, Syed Danish,Khanam, Aziza,Sultan, Naheed,Idrees, Farah,Akhtar, Naheed,Imtiaz, Fauzia Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2
Background: There are several validated risk factors for breast cancer. However the legitimacy of elevated fasting blood glucose (FBG) is not well established. This study was designed to assess this parameter as a risk factor for breast cancer among pre- and post-menopausal women. Materials and Methods: This case-control study was conducted at Department of Biochemistry, University of Karachi from June 2010 to August 2014. Simple random sampling technique was used to collect data of study subjects comprising 175 diagnosed breast cancer patients with positive histopathology from Breast Clinic, surgical unit-1, Civil Hospital, Karachi and 175 healthy controls from various screening programs. Blood samples were analyzed for FBG and serum insulin. Results: FBG, HOMA-IR, systolic and diastolic blood pressure were significantly raised in breast cancer cases when compared to control subjects. Cases and controls were further categorized in to two groups using cutoff value of 110mg/dl to distinguish subjects into normal fasting glucose (<110mg/dl) and having impaired fasting glucose (${\geq}110-{\leq}125mg/dl$) or diabetes (${\geq}126mg/dl$). Odds ratios were found to be 1.57, 2.15 and 1.17 in overall, pre-menopausal and post-menopausal groups, respectively. (all p < 0.05). Conclusions: A statistically significant risk of breast cancer exists in women having elevated fasting blood glucose levels, corresponding to prediabetes and diabetes, among pre and postmenopausal ages, with comparatively greater effects in the premenopausal group.
Serum Adiponectin Level Association with Breast Cancer Risk: Evidence from a Case-Control Study
Ahmed, Syed Danish Haseen,Khanam, Aziza,Sultan, Naheed,Idrees, Farah,Akhter, Naheed Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12
Background: Adiponectin is a protein, synthesized by adipose tissue, which has a questionable role in breast cancer pathogenesis. This study focused on serum levels of adiponectin among cases and controls, and its possible association with breast cancer risk. Materials and Methods: This case-control study covered 175 diagnosed breast cancer patients with positive histopathology from Breast Clinic, surgical unit-1, Civil Hospital, Karachi and 175 healthy controls from various screening programs. Blood samples were obtained after an overnight fast and later analyzed. Results: Fasting blood glucose was significantly raised, while serum adiponectin was significantly decreased in breast cancer cases when compared to control subjects. To find the precise influence of serum adiponectin on the risk of breast cancer, subjects were divided in to quartiles based on serum levels of adiponectin. It is observed that risk of breast cancer decreased with rising quartile. Q2 (25th-50th) had the highest OR=1.76 (CI: 0.93- 3.34), Q3 (50th-75th) has OR= 0.89 (CI: 0.48-1.64), while subjects in the highest quartile Q4 (>75th) had significantly (p< 0.05) decreased risk of breast cancer having OR=0.06 (CI: 0.02-0.15), when compared to the lowest quartile. Conclusions: We demonstrated a statistically significant association of elevated serum adiponectin with decreased risk of breast cancer. This signifies a protective role of adiponectin via an intricate mechanism of masking mitogenic growth factors in breast carcinogenesis.
New Butyrylcholinesterase Inhibitory Triterpenes from Salvia santolinifolia
Sajid Mehmood,Naheed Riaz,Sarfraz Ahmed Nawaz,Nighat Afza,Abdul Malik,Muhammad Iqbal Choudhary 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.3
Slavins A (1) and B (2), the new amyrin type triterpenes, have been isolated from the chloroform soluble fraction of Salvia santolinifolia and assigned structures on the basis of spectral studies including 2D NMR. Both the compounds displayed inhibitory potential against the enzyme butyrylcholinesterase.
Isbat, Mohammad,Zeba, Naheed,Kim, Seong Ryong,Hong, Choo Bong Elsevier 2009 Journal of plant physiology Vol.166 No.15
<P><B>Summary</B></P><P>Programmed cell death (PCD) is a highly conserved cellular suicide process important in developmental processes and elimination of damaged cells upon environmental stresses. Among the important regulators of PCD, much interest has been centered on BCL2-associated x protein (BAX) as the pro-PCD factor. On the other hand, BAX inhibitor-1 (BI-1) has been implicated as an anti-PCD factor that balances out the activity of BAX in the developmental processes and responses to environment. A cDNA clone coding a BI-1 gene was isolated from a cDNA library of heat-stressed hot pepper (<I>Capsicum annuum</I>) and named as <I>CaBI-1</I>. This gene contains an open reading frame (ORF) of 248 amino acids encoding a BI-1 protein. Genomic DNA-blot analysis for <I>CaBI-1</I> suggested one or two loci in the <I>C. annuum</I> genome. Transcription of <I>CaBI-1</I> was induced in response to high or low temperatures, drought, high salinity, flooding and heavy metal stresses, and ABA. We introduced the ORF of <I>CaBI-1</I> under the control of the CaMV 35S promoter (P<SUB>35S</SUB>) into tobacco (<I>Nicotiana tabacum</I> cv. Wisconsin 38) genome by <I>Agrobacterium</I>-mediated transformation. The P<SUB>35S</SUB>:<I>CaBI-1</I> transgenic plants displayed markedly improved tolerance to high temperature, water deficit, and high salinity in comparison to the control plants. The results indicate that <I>CaBI-1</I> is a BI-1 gene of which expression induced under various abiotic stresses and endows tolerance to several types of environmental stresses.</P>