Antidiabetic and antioxidant effect of methanol extract of edible plant Talinum portulacifolium (Forssk) in Streptozotocin induced diabetic rats

Nageswara Rao Thalapaneni,Mohana Lakshmi Sabapathi,Faraz Raza Ansari,Subhash C. Mandal 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3

The aim of this study is to evaluate antidiabetic and antioxidant effects of methanol extract of Talinum portulacifolium (FORSSK) leaves (Portulacaceae) (METP) in streptozotocin induced diabetic rats. The acute and sub acute antihyperglycemic effect of two different doses (200 and 400 mg/kg,) of METP were investigated. Blood glucose level, serum insulin level, lipid profiles and biomarker enzymes were evaluated in normal and diabetic rats. Liver glycogen content, kidney and liver antioxidant enzymes were evaluated. Supplementation of METP by oral gavage significantly reduces the fasting blood glucose level. This supplementation significantly increases the glycogen level in the liver (32.33±1.453 mg/gm) as compare to diabetic control group (5.66±0.881 mg/gm) (p<0.001). Elevated serum glutamate oxaloacetate transaminase (SGOT), glutamate pyruvate transaminase (SGPT), and alkaline phosphatase (SALP) were decreased significantly by the treatment of METP with respect to diabetic group. The antioxidant activity was studied in liver and kidney tissues by measuring malondialdehyde (MDA), reduced glutathione (GSH) and catalase levels. In the METP treated groups, the HDL/LDL ratio was improved and cholesterol and triglycerides levels also decreased significantly. Histopathological studies of the pancreas of these animals showed comparable regeneration by METP. Glibenclamide was used as a standard at a dose of 0.5 mg/kg p.o

Selective Ring Opening of Methylcyclopentane and Methylcyclohexane Over Iridium Bifunctional Catalysts Supported on Surface Modified γ-Al2O3, SiO2 and Ultra Stable Y Zeolites

Nageswara Rao, R.,You, Nansuk,Yoon, Songhun,Upare, Dipali Pravin,Park, Yong-Ki,Lee, Chul Wee Springer-Verlag 2011 Catalysis letters Vol.141 No.7

Antioxidant Activity of Talinum portulacifolium (Forssk) Leaf Extracts

Thalapaneni Nageswara Rao,Chidambaram Kumarappan,Sabapathi Mohana Lakshmi,Subhash C Mandal 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.4

The aim of the study is to investigate the antioxidant activity through, reducing power, 2, 2- diphenyl-1-picryl hydrazyl radical (DPPH), nitric oxide radical (NO), superoxide radical, hydrogen peroxide radical (H2O2) scavenging activity and the amount of total phenolic compounds of chloroform, ethyl acetate, methanol and aqueous extracts of the leaves of Talinum portulacifolium. Chloroform extract of leaves of T. portulacifolium showed highest antioxidant activity, with a direct relationship between activity and concentration of extracts (15 - 240 μg/mL). Among all the extracts, the highest amount of the total polyphenolic compounds was found in the chloroform extract. Chloroform extract of T. portulacifolium showed an important free radical scavenging activity towards the DPPH, NO, Superoxide and H2O2 radicals, with IC50 values of 133.26, 165.75, 156.34 and 135.29 μg/mL, respectively. In the lipid peroxidation assay, extracts of chloroform and ethyl acetate showed a remarkable inhibitory activity. The extracts showed significant activity in all the experiments but lower than the standard antioxidant, ascorbic acid. The aim of the study is to investigate the antioxidant activity through, reducing power, 2, 2- diphenyl-1-picryl hydrazyl radical (DPPH), nitric oxide radical (NO), superoxide radical, hydrogen peroxide radical (H2O2) scavenging activity and the amount of total phenolic compounds of chloroform, ethyl acetate, methanol and aqueous extracts of the leaves of Talinum portulacifolium. Chloroform extract of leaves of T. portulacifolium showed highest antioxidant activity, with a direct relationship between activity and concentration of extracts (15 - 240 μg/mL). Among all the extracts, the highest amount of the total polyphenolic compounds was found in the chloroform extract. Chloroform extract of T. portulacifolium showed an important free radical scavenging activity towards the DPPH, NO, Superoxide and H2O2 radicals, with IC50 values of 133.26, 165.75, 156.34 and 135.29 μg/mL, respectively. In the lipid peroxidation assay, extracts of chloroform and ethyl acetate showed a remarkable inhibitory activity. The extracts showed significant activity in all the experiments but lower than the standard antioxidant, ascorbic acid.

Deletion of GSTM1 and T1 Genes as a Risk Factor for Development of Acute Leukemia

Dunna, Nageswara Rao,Vure, Sugunakar,Sailaja, K.,Surekha, D.,Raghunadharao, D.,Rajappa, Senthil,Vishnupriya, S. Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

The glutathione S-transferases (GSTs) are a family of enzymes involved in the detoxification of a wide range of chemicals, including important environmental carcinogens, as well as chemotherapeutic agents. In the present study 294 acute leukemia cases, comprising 152 of acute lymphocytic leukemia (ALL) and 142 of acute myeloid leukemia, and 251 control samples were analyzed for GSTM1 and GSTT1 polymorphisms through multiplex PCR methods. Significantly increased frequencies of GSTM1 null genotype (M0), GSTT1 null genotype (T0) and GST double null genotype (T0M0) were observed in the both ALL and AML cases as compared to controls. When data were analyzed with respect to clinical variables, increased mean levels of WBC, Blast %, LDH and significant reduction in DFS were observed in both ALL and AML cases with T0 genotype. In conclusion, absence of both GST M & GST T might confer increased risk of developing ALL or AML. The absence of GST enzyme might lead to oxidative stress and subsequent DNA damage resulting in genomic instability, a hallmark of acute leukemia. The GST enzyme deficiency might also exert impact on clinical prognosis leading to poorer DFS. Hence GST genotyping can be made mandatory in management of acute leukemia so that more aggressive therapy such as allogenic stem cell transplantation may be planned in the case of patients with a null genotype.

Climate change impact analysis on watershed using QSWAT

N. Nageswara Reddy,K. Venkata Reddy,J. Sri Lakshmi Sesha Vani,Prasad Daggupati,Raghavan Srinivasan 대한공간정보학회 2018 Spatial Information Research Vol.26 No.3

This study focuses on analysis of future climate change impacts on West Nishnabotna Watershed, using climate model data and an open source hydrological model—Soil and Water Assessment Tool (SWAT), which is run in Quantum Geographical Information System( QGIS) environment. The data required to simulate hydrological processes using SWAT plugin in QGIS (QSWAT) are Digital Elevation Model, land use/landcover map, soil map, slope map and climate data of the study area. The required climate parameters like maximum temperature, minimum temperature and precipitation are obtained from North American Regional Climate Change Assessment Program climate model database which are bias corrected for the study area using linear scaling method. SWAT model is setup using observed climate and the model calibration and validation is performed using SWAT Calibration and Uncertainty Program (SWATCUP). Simulations are run for historic (1971–1999) and future (2041–2069) climate conditions for the stream flow prediction. The climate change impact analysis like anomalies and trends in the hydrological regime of the watershed are carried out based on QSWAT simulation results.

Antidiabetic and antioxidant effect of methanol extract of edible plant $Talinum$ $portulacifolium$ (Forssk) in Streptozotocin induced diabetic rats

Thalapaneni, Nageswara Rao,Sabapathi, Mohana Lakshmi,Ansari, Faraz Raza,Mandal, Subhash C. 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.3

The aim of this study is to evaluate antidiabetic and antioxidant effects of methanol extract of $Talinum$ $portulacifolium$ (FORSSK) leaves (Portulacaceae) (METP) in streptozotocin induced diabetic rats. The acute and sub acute antihyperglycemic effect of two different doses (200 and 400 mg/kg,) of METP were investigated. Blood glucose level, serum insulin level, lipid profiles and biomarker enzymes were evaluated in normal and diabetic rats. Liver glycogen content, kidney and liver antioxidant enzymes were evaluated. Supplementation of METP by oral gavage significantly reduces the fasting blood glucose level. This supplementation significantly increases the glycogen level in the liver ($32.33{\pm}1.453$ mg/gm) as compare to diabetic control group ($5.66{\pm}0.881$ mg/gm) ($p$ <0.001). Elevated serum glutamate oxaloacetate transaminase (SGOT), glutamate pyruvate transaminase (SGPT), and alkaline phosphatase (SALP) were decreased significantly by the treatment of METP with respect to diabetic group. The antioxidant activity was studied in liver and kidney tissues by measuring malondialdehyde (MDA), reduced glutathione (GSH) and catalase levels. In the METP treated groups, the HDL/LDL ratio was improved and cholesterol and triglycerides levels also decreased significantly. Histopathological studies of the pancreas of these animals showed comparable regeneration by METP. Glibenclamide was used as a standard at a dose of 0.5 mg/kg p.o.

High Affinity Pharmacological Profiling of Dual Inhibitors Targeting RET and VEGFR2 in Inhibition of Kinase and Angiogeneis Events in Medullary Thyroid Carcinoma

Dunna, Nageswara Rao,Kandula, Venkatesh,Girdhar, Amandeep,Pudutha, Amareshwari,Hussain, Tajamul,Bandaru, Srinivas,Nayarisseri, Anuraj Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Clinical evidence shows that dual inhibition of kinases as well angiogenesis provides ideal therapeutic option in the treatment of medullary thyroid carcinoma (MTC) than inhibiting either of these with the events separately. Although treatment with dual inhibitors has shown good clinical responses in patients with MTC, it has been associated with serious side effects. Some inhibitors are active agents for both angiogenesis or kinase activity. Owing to narrow therapeutic window of established inhibitors, the present study aims to identify high affinity dual inhibitors targeting RET and VEGFR2 respectively for kinase and angiogenesis activity. Established inhibitors like Vandetanib, Cabozantinib, Motesanib, PP121, RAF265 and Sunitinib served as query parent compounds for identification of structurally similar compounds by Tanimoto-based similarity searching with a threshold of 95% against the PubChem database. All the parent inhibitors and respective similar compounds were docked against RET and VEGFR2 in order to retrieve high affinity compounds with these two proteins. AGN-PC-0CUK9P PubCID: 59320403 a compound related to PPI21 showed almost equal affinity for RET and VEGFR2 and unlike other screened compounds with no apparent bias for either of the receptors. Further, AGNPC- 0CUK9P demonstrated appreciable interaction with both RET and VEGFR2 and superior kinase activity in addition to showed optimal ADMET properties and pharmacophore features. From our in silico investigation we suggest AGN-PC-0CUK9P as a superior dual inhibitor targeting RET and VEGFR2 with high efficacy which should be proposed for pharmacodynamic and pharmacokinetic studies for improved treatment of MTC.

A Study of the Agile Software Development Methods, Applicability and Implications in Industry

Kuda Nageswara Rao,G. Kavita Naidu,Praneeth Chakka 보안공학연구지원센터 2011 International Journal of Software Engineering and Vol.5 No.2

TP53 Codon 72 Polymorphism and Risk of Acute Leukemia

Dunna, Nageswara Rao,Vure, Sugunakar,Sailaja, K.,Surekha, D.,Raghunadharao, D.,Rajappa, Senthil,Vishnupriya, S. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

TP53 is the mostly commonly mutated gene in many cancers and the P53 tumor suppressor protein is involved in multiple cellular processes, including transcription, DNA repair, genomic stability, senescence, cell cycle control and apoptosis. A common single nucleotide polymorphism located within the proline rich region of TP53 gene at codon 72 in exon 4 encodes either proline or arginine. TP53 Arg 72 is more active than TP53 Pro 72 in inducing apoptosis. The aim of this study was to understand the association of the 72 codon polymorphism with acute leukemia development and prognosis. A total of 288 acute leukemia cases comprising 147 acute lymphocytic leukemia (ALL) and 141 acute myeloid leukemia (AML), as well as 245 controls were recruited for analysis of the TP53 72 polymorphism using PCR-RFLP method. Significant association of homozygous arginine genotype with AML was observed (${\chi}^2$- 133.53; df-2, p < 0.001. When data were analyzed with respect to clinical variables, elevation in mean WBC, blast %, LDH levels and slight reduction in DFS in ALL cases with the arginine genotype was observed. In contrast, AML patients with Pro/Pro had elevated WBC, Blast%, LDH levels with slightly reduced DFS. Our study indicates that Arg/Arg genotype might confer increased risk to development of acute myeloid leukemia.

Thermal resistance effect of graphene doped zinc oxide nanocomposite in fire retardant epoxy coatings

Tentu Nageswara Rao,Imad Hussain,Riyazuddin,Bon Heun Koo 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.4

Graphene doped zinc oxide nanoparticles (G-ZnO) were prepared using modified hummer’s technique together with the ultrasonic method and characterized by field emission scanning electron microscopy (FESEM), X-ray powder diffraction (XRD), fourier-transform infrared spectroscopy (FTIR) and high-resolution transmission electron microscopy (HRTEM). Different samples of epoxy resin nanocomposites reinforced with G-ZnO nanoparticles were prepared and were marked as F1 (without adding nanoparticles), F2 (1% w/w G-ZnO), and F3 (2% w/w G-ZnO) in combination of ≈ 56:18:18:8w/w% with epoxy resin/hardener, ammonium polyphosphate, boric acid, and Chitosan. The peak heat release rate (PHRR) of the epoxy nanocomposites was observed to decrease dramatically with the increasing G-ZnO nanoparticles. However, the LOI values increased significantly with the increase in wt % of G-ZnO nanoparticles. From the UL-94V data, it was confirmed that the F2 and F3 samples passed the flame test and were rated as V-0. The results obtained in the present work clearly revealed that the synthesized samples can be used as efficient materials in fire-retardant coating technology.