p16INK4a immunohistochemistry is a promising biomarker to predict the outcome of low grade cervical intraepithelial neoplasia: comparison study with HPV genotyping

Sakiko Nishio,Takuma Fujii,Hiroshi Nishio,Kaori Kameyama,Miyuki Saito,Takashi Iwata,Kaneyuki Kubushiro,Daisuke Aoki 대한부인종양학회 2013 Journal of Gynecologic Oncology Vol.24 No.3

Objective: In cervical intraepithelial neoplasia (CIN), p16INK4a immunohistochemistry has been reported to be a useful diagnostic biomarker. However, limited information is available about the association between the p16INK4a immunohistochemistry and the outcomes of CIN. Here, we report p16INK4a immunohistochemistry as an effective biomarker to predict the outcomes of CIN. Methods: p16INK4a immunohistochemistry was performed in patients with CIN from January 2000 to August 2009. Among these patients, we have performed a retrospective analysis of the medical records to evaluate the outcome of CIN 1-2 and performed statistical analysis to determine the correlation between p16INK4a expression and the outcomes. We also performed HPV genotyping and analyzed the relation between the infecting human papillomavirus (HPV) genotype and the outcomes. Results: A total of 244 patients, including 82 with CIN 1, 60 with CIN 2, and 102 with CIN 3, were examined. The rate of p16INK4a overexpression increased with increasing CIN grade, 20.7% for CIN 1, 80.0% for CIN 2, and 89.2% for CIN 3, with significant differences between CIN 1 and CIN 2-3 group. In the 131 CIN 1-2 patients, the progression rate was significantly higher for the patients showing p16INK4a overexpression than for those not showing p16INK4a overexpression (p=0.005); the regression rate was also found to be significantly lower for the patients showing p16INK4a overexpression (p=0.003). High-risk HPV genotypes were detected in 73 patients (73.7%). Both progression and regression rates were not significantly different between the high-risk HPV-positive and HPV-negative groups (p=0.401 and p=0.381, respectively). Conclusion: p16INK4a overexpression was correlated with the outcome of CIN 1-2, and p16INK4a is considered to be a superior biomarker for predicting the outcome of CIN 1-2 compared with HPV genotyping.

편측구순열 1차수술

NISHIO Juntaro,ADACHI Tadafumi,KASHIMA Yukiko 대한구순구개열학회 2000 대한구순구개열학회지 Vol.3 No.2

The alar base on the cleft side in unilateral complete cleft lip, alveolus and palate is markedly displaced laterally, caudally and dorsally, By incising the pyriform margin from the cleft margin of the alveolar process, including mucosa of the anterior part of the inferior turbinate, to the upper end of the postnasal vestibular fold, the alar base is released from the maxilla, A physiological correction of nasal deformity can be accomplished by careful reconstruction of nasolabial muscle integrity, functional repair of the orbicular muscle, raising and rotating the displaced alar cartilage, and finally by lining the lateral nasal vestibule, The inferior maxillary head of the nasal muscle complex is identified as the deeper muscle just below the web of the nostril, The muscle is repositioned inframedially, so that it is sutured to the periosteum that overlies the facial aspect of the premaxilla in the region of the developing lateral incisor tooth, And then, the deep superior part of the orbicular muscle is sutured to the periosteum and the fibrous tissue at the base of the septum, just in front of the anterior nasal spine, The nasal floor is surgically created by insertions of the nasal muscle complex in deep plane and of the orbicular muscle in superficial one, The upper part of the lateral nasal vestibular defect is sutured by shifting the alar flap cephalically, The middle and lower parts of this defect are closed by use of cleft margin flaps of the philtral and lateral segments, respectively, Authors stress the importance of nasal floor reconstruction at primary surgery and report the technique and postoperative results.

Preparation and Properties of Inorganic-organic Hybrid $Li^+$ Ion Conductor by Sol-gel Process

Nishio, Keishi,Miyazawa, Tsutomu,Watanabe, Yuichi,Tsuchiya, Toshio The Korean Ceramic Society 2001 The Korean journal of ceramics Vol.7 No.1

Inorganic-organic hybrid Li$^+$ ion conductors were prepared by the sol-gel process. Tetramethyl orthosilicate (TMOS), polyethylene glycol 200 (PEG$_200$) and lithium bis (trifluoro-methylsulfony) imide were used as raw materials and $H_2O$ was used as a solvent. Hybrid Li$^+$ ion conductor prepared by the sol-gel process showed very high ion conductivities of log${\sigma}_R.T$(S/cm)=-3.73, log${\sigma}_60$(S/cm)=-3.00 at room temperature and $60^{\circ}C$, respectivery. Decomposition voltage was 3.1 V.

Higher-Order Evaluation Method for Dynamic PIV

Nishio S. 한국가시화정보학회 2006 KOREA-JAPAN Joint Seminar on Particle Image Veloci Vol.- No.-

한국의 지방자치에 거는 기대

Nishio Akira 한국지방자치연구소 1991 自治硏究 Vol.1 No.2

Sea Ice Extents and global warming in Okhotsk Sea and surrounding Ocean

Nishio, Fumihiko 대한원격탐사학회 1998 International Symposium on Remote Sensing Vol.14 No.1

Increase of greenhouse gas due to CO₂ and CH., gases would cause the global warming in the atmosphere. According to the global circulation model, it is pointed out in the Okhotsk Sea that the large increase of atmospheric temperature might be occurred in this region by global warming due to the doubling of green house effect gases. Therefore, it is very important to monitor the sea ice extents in the Okhotsk Sea. To improve the sea ice eartents and concentration with more highly accuracy, the field experiments have begun to compare with Airborne Microwave Radiometer (AMR) and video images installed on their craft(Beach-200). The sea ice concentration is generally proportional to the brightness temperature and accurate retrieval of sea ice concentration from the brightness temperature is important because of the sensitivity of multi-channel data with the amount of open water in the sea ice pack. During the field experiments of airborned AMR, the multi-frequency data suggest that the sea ice concentration is slightly dependending on the sea ice types since the brightness temperature is different between the thin and small piece of sea ice floes, and a large ice flow with different surface signatures. On the basis of classification of two sea ice types, it is clearly distinguished between the thin ice and the large ice floe in the scatter plot of36.E and 89.0GHz, but it does not become to make clear of the scatter plot of 18.7 and 36.5GHz Two algorithms that have been used for deriving sea ice concern rations from airborned multi-channel data are compared. One is the NASA Team Algorithm and the other is the Bootstrap Algorithm. Intercomparison on both algorithms with the airborned data and sea ice concentration derived from video images bas shown that the Bootstrap Algorithm is more consistent with the binary maps of video images.

敎育自治의 觀點에서 본 分權 - 現代 日本의 敎育行政과 未來敎育의 課題 -

Nishio Masaru 한국지방자치연구소 1994 自治硏究 Vol.4 No.3

Large conformational changes in the <i>Escherichia coli</i> tryptophan synthase β₂ subunit upon pyridoxal 5′‐phosphate binding

Nishio, Kazuya,Ogasahara, Kyoko,Morimoto, Yukio,Tsukihara, Tomitake,Lee, Soo Jae,Yutani, Katsuhide Blackwell Publishing Ltd 2010 FEBS JOURNAL Vol.277 No.9

<P>To understand the basis for the lower activity of the tryptophan synthase β<SUB>2</SUB> subunit in comparison to the α<SUB>2</SUB>β<SUB>2</SUB> complex, we determined the crystal structures of apo‐β<SUB>2</SUB> and holo‐β<SUB>2</SUB> from <I>Escherichia coli</I> at 3.0 and 2.9 Å resolutions, respectively. To our knowledge, this is the first report of both β<SUB>2</SUB> subunit structures with and without pyridoxal‐5′‐phosphate. The apo‐type molecule retained a dimeric form in solution, as in the case of the holo‐β<SUB>2</SUB> subunit. The subunit structures of both the apo‐β<SUB>2</SUB> and the holo‐β<SUB>2</SUB> forms consisted of two domains, namely the N domain and the C domain. Although there were significant structural differences between the apo‐ and holo‐structures, they could be easily superimposed with a 22° rigid body rotation of the C domain. The pyridoxal‐5′‐phosphate‐bound holo‐form had multiple interactions between the two domains and a long loop (residues 260–310), which were missing in the apo‐form. Comparison of the structures of holo‐<I>Ec</I>β<SUB>2</SUB> and <I>St</I>β<SUB>2</SUB> in the α<SUB>2</SUB>β<SUB>2</SUB> complex from <I>Salmonella typhimurium</I> (<I>St</I>α<SUB>2</SUB>β<SUB>2</SUB>) identified the cause of the lower enzymatic activity of holo‐<I>Ec</I>β<SUB>2</SUB> in comparison with <I>St</I>α<SUB>2</SUB>β<SUB>2</SUB>. The substrate (indole) gate residues, Tyr279 and Phe280, block entry of the substrate into the β<SUB>2</SUB> subunit, although the indole can directly access the active site as a result of a wider cleft between the N and C domains in the holo‐<I>Ec</I>β<SUB>2</SUB> subunit. In addition, the structure around βAsp305 of the holo‐<I>Ec</I>β<SUB>2</SUB> subunit was similar to the open state of <I>St</I>α<SUB>2</SUB>β<SUB>2</SUB> with low activity, resulting in lower activity of holo‐<I>Ec</I>β<SUB>2</SUB>.</P><P><B>Structured digital abstract</B></P><P><P>: <I>Ecβ2</I> (uniprotkb:) and <I>Ecβ2</I> (uniprotkb:) <I>bind </I>() by <I>x‐ray crystallography</I> ()</P><P>: <I>Ecβ2</I> (uniprotkb:) and <I>Ecβ2</I> (uniprotkb:) <I>bind </I>() by <I>biophysical</I> ()</P></P>

Emotional Burden and Their Correlated Factors about Type 1 Diabetes Patients Using Insulin Pump Therapy

Ikuko Nishio,Masami Chujo 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

Development of higher performance algorithm for dynamic PIV

Shigeru NISHIO 한국가시화정보학회 2004 KOREA-JAPAN Joint Seminar on Particle Image Veloci Vol.- No.-

The new algorithm for higher performance of dynamic PIV has been proposed. Present study considered mathematical basis of PIV analysis for multiple-time-step images and it enables us to analyze the high time-resolution PIV, which is obtained by dynamic PlV system. Conventional single pair image PIV analysis gives us the velocity field data in each time step but it sometimes contains unnecessary information of target flow. Present technique utilize multi-time step correlation information, and it is analyzed