One-step Purification and Immobilization of His-tagged Protein via Ni2+-functionalized Fe3O4@Polydopamine Magnetic Nanoparticles

Jianbing Yang,Kefeng Ni,Dongzhi Wei,Yuhong Ren 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

Ni2+-functionalized Fe3O4@polydopamine magnetic nanoparticles (Ni2+-PD-MNPs) were designed and synthesized by in situ coating of magnetic nanoparticles with polydopamine, followed by conjugation of Ni2+ to the polydopamine film. The Ni2+-PD-MNPs were used to purify His-tagged red fluorescent protein (His-RFP) via affinity interaction between Ni2+ and the His-tag. The results showed that the Ni2+-PD-MNPs had extraordinary selectivity for His-RFP purification. In addition, a Histagged transaminase (ω-transaminase BJ110) was selectively immobilized onto the Ni2+-PD-MNPs without purification, and the immobilized enzyme showed improved specific activity, as well as enhanced stability and reusability.

Site Preference of Alloying Elements in DO22-Ni3V Phase: Phase-Field and First-Principles Study

Ding-Ni Zhang,Qian-Qian Shangguan,Fu Liu,Ming-Yi Zhang 대한금속·재료학회 2015 METALS AND MATERIALS International Vol.21 No.4

Site preference of alloying elements in DO22-Ni3V phase was investigated using phase-field and first-principles method. The concentrations of alloying elements on sublattices of DO22-Ni3V phase were quantitatively studied using phase-field model based on microscopic diffusion equations. The phase-field computation results demonstrate that the concentration differences of alloying elements on the NiI and NiII site are attributed to the coordination environment difference. Host atoms Ni and substitutional ternary additions Al prefer to occupy NiI site. Antisite atoms V show site preference on the NiII site. Further reason of site preference of alloying elements on the two different Ni sites were studied using first-principles method to calculate the electronic structure of DO22-Ni3V phase. Calculation of density of states, orbitals population and charge population of the optimized Ni3V structure found that the electronic structures of NiI and NiII sites are different. Electronic structure difference, which is caused by coordination environment difference, is the essential reason for site selectivity behaviors of alloying elements on NiI and NiII sites.

Structural, magnetic and dielectric properties of (Li1+, Al3+) co-doped Ni0.5Zn0.5Fe2O4 ferrite ceramics prepared by the sol-gel auto-combustion method

Qing Ni,Li Sun,Ensi Cao,Wentao Hao,Yongjia Zhang,Lin Ju 한국물리학회 2020 Current Applied Physics Vol.20 No.9

(Li1+, Al3+) co-doped Ni0.5Zn0.5Fe2O4 ferrites, Ni0.5-xZn0.5-xLixAlxFe2O4 (x = 0.000, 0.025, 0.050 and 0.100), were synthesized by the sol-gel auto-combustion method. X-ray diffraction (XRD), field emission scanning electronic microscope (FESEM), vibrating sample magnetometer (VSM) and LCR meter were used to investigate the structural, magnetic and dielectric properties. Results of XRD and SEM indicate that both doping amount and calcination temperature play significant roles in crystal structure and grain growth. Also, it can be observed that the saturation magnetization and the coercivity change in a noticeable manner. The Ni0.475Zn0.475Li0.025Al0.025Fe2O4 ferrite sintered at 1200 °C has a relatively low coercivity value (62.93 Oe) and the largest saturation magnetization (110.95 emu/g). Besides, dielectric behavior is also improved by Li1+ and Al3+ co-doping

The structural and magnetic properties of Ni₄₅Al₄₅C₁₀ solid solution as a function of milling times

Kontan Tarigan,Yong-Goo Yoo,Dong-Seok Yang,Ni Luh Karina Kartika,Seong-Cho Yu 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

We have studied the formation of alloy for Ni₄?Al₄?C₁? as a function of milling times. This alloy was produced using mechanical alloying. The effect of milling time on local structural changes of Ni₄?Al₄?C₁? has been investigated by means of EXAFS. Both XRD and EXAFS patterns from mechanically alloyed Ni₄?Al₄?C₁? powder indicates the formation of solid solution. The variation of lattice parameter and particle sizes could be analyzed from the different of milling times. Magnetization was also measured by using VSM.

SOFC의 세라믹 음극물질로서 Y_0.08Sr_0.92Fe_0.3Ti_0.7O₃의 합성 및 물성 평가

Lee, Tae-Hee,Jeon, Sang-Yun,Im, Ha-Ni,Song, Sung-Ju 한국전력공사 2021 KEPCO Journal on electric power and energy Vol.7 No.1

In general, SOFCs mainly use Ni-YSZ cermet, a mixture of Ni and YSZ, as an anode material, which is stable in a high-temperature reducing atmosphere. However, when SOFCs have operated at a high temperature for a long time, the structural change of Ni occurs and it results in the problem of reducing durability and efficiency. Accordingly, a development of a new anode material that can replace existing nickel and exhibits similar performance is in progress. In this study, SrTiO₃, which is a perovskite-based mixed conductor and one of the candidate materials, was used. In order to increase the electrical conduction properties, Y_0.08Sr_0.92Fe_0.3Ti_0.7O₃, doped with 0.08 mol of Y³⁺ in Sr-site and 0.03 mol of transition metal Fe³⁺ in Ti-site, was synthesized and its chemical diffusion coefficient and reaction constant were measured. Its electrical conductivity changes were also observed while changing the oxygen partial pressure at a constant temperature. The performance as a candidate electrode material was verified by predicting the defect area through the electrical conductivity pattern according to the oxygen partial pressure.

General template-free strategy for fabricating mesoporous two-dimensional mixed oxide nanosheetsviaself-deconstruction/reconstruction of monodispersed metal glycerate nanospheres

Kaneti, Yusuf Valentino,Salunkhe, Rahul R.,Wulan Septiani, Ni Luh,Young, Christine,Jiang, Xuchuan,He, Yan-Bing,Kang, Yong-Mook,Sugahara, Yoshiyuki,Yamauchi, Yusuke The Royal Society of Chemistry 2018 Journal of materials chemistry. A, Materials for e Vol.6 No.14

<P>In this work, we propose a general template-free strategy for fabricating two-dimensional mesoporous mixed oxide nanosheets, such as metal cobaltites (MCo2O4, M = Ni, Zn) through the self-deconstruction/reconstruction of highly uniform Co-based metal glycerate nanospheres into 2D Co-based metal glycerate/hydroxide nanosheets, induced by the so-called “water treatment” process at room temperature followed by their calcination in air at 260 °C. The proposed ‘self-deconstruction/reconstruction’ strategy is highly advantageous as the resulting 2D metal cobaltite nanosheets possess very high surface areas (150-200 m<SUP>2</SUP>g<SUP>−1</SUP>) and mesoporous features with narrow pore size distribution. In addition, our proposed method also enables the crystallization temperature to achieve pure metal cobaltite phase from the precursor phase to be lowered by 50 °C. Using the 2D mesoporous NiCo2O4nanosheets as a representative sample, we found that they exhibit 6-20 times higher specific capacitance and greatly enhanced capacitance retention compared to the NiCo2O4nanospheres achieved through the direct calcination of the Ni-Co glycerate nanospheres. This highlights another advantage of the proposed strategy for enhancing the electrochemical performance of the mixed oxide products for supercapacitor applications. Furthermore, the asymmetric supercapacitor (ASC) assembled using the 2D NiCo2O4nanosheets//graphene oxide (GO) exhibits a maximum energy density of 38.53 W h kg<SUP>−1</SUP>, while also showing a high capacitance retention of 91% after 2000 cycles at 5 A g<SUP>−1</SUP>. It is expected that the proposed general method may be extended to other transition metal elements for creating 2D mixed oxide nanosheets with enhanced surface areas and improved electrochemical performance.</P>

Sustainability Issues in Juvenile Fishing in the Philippines

Niño Alejandro Q. Manalo,Paul Joseph B. Ramirez 한국무역연구원 2011 The International Academy of Global Business and T Vol.7 No.2

The study examines the problem of juvenile fishing as applied to anchovies, sardines, siganids (rabbitfish), and tuna fisheries in the Philippines and discusses the issues revolving around the sustainability of such fisheries in the long run. Results of the study showed that overfishing has resulted in the overexploitation of adult anchovies, sardines, rabbitfish, and tuna, and that juvenile fishing exacerbates the problem. The Philippines already has an enabling law that promotes the protection and conservation of Philippine fisheries resources. The provisions of the 1998 Philippine Fisheries Code range from regulating mesh sizes and types of fishing gear used to the establishment of marine protected areas and closed fishing seasons. Like any piece of legislation, enforcement accompanied by community stakeholder participation is crucial in achieving the sustainability of fisheries resources in the long term.

Diversity and threats to cave-dwelling bats in a small island in the southern Philippines

Ma. Niña Regina M. Quibod,Phillip A. Alviola,Anna Pauline O. de Guia,Virginia C. Cuevas,Ireneo L. Lit Jr.,Bonifacio O. Pasion 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.4

More than 2,000 caves have been documented in the Philippines, yet cave research is very limited. Thisstudy was conducted to provide benchmark information on the diversity and ecology of cave-dwellingbats from Samal Island, a small island in the southern Philippines. Bats were surveyed from thirtycaves of varying physical features and disturbances. Fifteen species of bats were identified in the island;14 of which are new records for the island, increasing the island distribution of the identified bats in thecountry. The abundance of cave-dwelling bats varied from low to high density, with some caves estimatedto harbor more than 10,000 individuals. Abundance and richness of cave-dwelling bats positivelyincreased with longer cave length, higher ceilings, bigger and multiple entrances, and presence of water. The Bat Cave Vulnerability Index (BCVI) revealed three high priority caves, 12 medium priority caves and15 low priority caves, indicating the importance of standardized method in assessing cave disturbance. Traces of tourism and hunting were the most common disturbance factors. The results of this studyhighlight the need for fundamental data on the distribution, diversity, and ecology of cave-dweling batsin the Philippines.

Distraction or Instruction: The Role of Online Interactive Games in a Child’s Second Language Acquisition

Ma. Niña I. Adriano 아시아영어교육학회 2017 The Journal of Asia TEFL Vol.14 No.3

Genome‑wide identification and molecular characterization of cysteine protease genes in rice

Marjohn C. Niño,Me‑Sun Kim,Kwon-Kyoo Kang,조용구 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Cysteine protease activity comprises the majority of proteolytic activities in plants. They are involved in almost every facet of the plant’s development. Accumulating evidence indicates multiple roles of this protease type in response to biotic and abiotic stress. To understand the regulations and functions of cysteine protease in rice, its evolutionary and structural evidence was uncovered in this study. Using MEROPS, a peptidase database, the 74 rice cysteine proteases belonging to six families were queried. Each of these families represents distinct proteolytic enzyme; C1 is a papain-like protease, C2 is a calpain- 2-type, C12 is an ubiquitinyl hydrolase-L1 enzyme, C13 is legumain, C14 is a caspase-1 type, and C15 is a pyroglutamyl peptidase 1 enzyme type. Evolutionary expansion attributed to gene duplication and diversification was particularly evident in C1 family which showed the highest number (n = 53) of members, most of which contained the highest number and most variable introns and motifs, whereas families C13, C14, and C15 had only a few members which all contained lesser number and variation of intron and motif. Out of 74 total cysteine protease gene members, 73 were globular proteins and 55 were predicted as stable proteins. Spatial expression assay of selected C1 members showed that LOC_Os01g73980 and LOC_Os05g01810 were highly expressed in the stem and leaves, while LOC_Os02g27030 was constitutively expressed in all tissues. The expression of LOC_Os01g73980 and LOC_Os05g01810 was also highly activated by salinity stress, while LOC_Os02g27030 was activated by both salinity and heat. LOC_Os05g01810 overexpression transgenic rice exhibited moderate tolerance to salinity stress, which provides interesting clues on biological functions of these genes in rice.