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N.K.Chaudhury,R.Bhardwaj,B.M.Murari 한국물리학회 2003 Current Applied Physics Vol.3 No.2, 3
Development of optical biosensors is an active area of research in the field of medical technology. Solgel matrices made fromalkoxide silicates,tetraethyl orthosilicate (TEOS) appear to be suitable glassy host matrix for the sensing system. However,themajor problem in the TEOS based solgel matrices is stability. So it is important to study dopantmatrix interaction as a function of time. In the present study,we report uorescence emission and excited state lifetime measurements on uorescent probes entrappedin TEOS solgel for monitoring the physico-chemical processes for characterization and monitoring of local environment (pores) ofviz.,Hoechst 33258 (H258) and pyranine (PY) were used. Solgels containing these probes were prepared at pH¼ 6:0 and thephysical and spectroscopic parameters were monitored as a function of storage time (days). The emission intensity from entrappedH258 has shown relatively higher extent of decrease during aging. The excited state uorescence lifetime measurements on theseprobes depicted single exponential decay component at 5.4 ns (PY) and 3.6 ns (H258) in fresh solgels. After a few days of storageprobe molecule PY. Further conrmation of multicomponents decay was obtained by distribution analysis of lifetime of H258where an increase in width of mean lifetime was observed with storage whereas no such change was indicated from PY. Thus itappears that H258 is a better probe molecule for characterizing and monitoring local environment of pores in solgel.. 2002 Elsevier Science B.V. All rights reserved.
Chaudhuri, A.,Roy, G.C.,Krishnan, N.,Sengupta, A.K.,Sen, S.K.,Saratchandra, B. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2
Biometabolic assessment was made in early and late embryonic stage (just before hatching) of one poly, one bi and their hybrids (DP, YPe, DP ${\times} Ype, and YPe ${\times} DP respectively ) of Bombyx mori to observe the racial differences. Protein and nucleic acid (RNA and DNA) concentrations were recorded to be significantly higher in bivoltine breed (YPe) and also in the hybrid than the polyvoltine (DP) strain in both the stages of embryonic development. The single egg weight of polyvoltine race was lower as compared to that of bivoltine and the hybrid studied. Age specific changes in all the biomolecules were evident where protein and RNA concentrations were elevated sharply in prehatched larvae while in case of DNA it was observed to be just reverse. The differences in protein, RNA and DNA composition between breeds and hybrids reflect the racial variations in biometabolic demands responsible for differential growth and development of the breeds and hybrids.
Chaudhuri, A.,Krishnan, N.,Roy, G.C.,Sengupta, A.K.,Sen, S.K.,Saratchandra, B. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2
Variations in protein and nucleic acid concentrations were observed in 24 hrs old eggs and hatched larvae of Nistari strain, Bombyx mori, exposed to starvation. Three starvation treatments of 24,48 and 60 hrs were given separately from 0 hr old fifth instar larvae. Biochemical variations were studied in the resultant hatched larvae of one time starved parent, while the eggs obtained from parents receiving starvation in two successive generations were considered for the study. In hatched larvae, protein levers in 24 hrs starvation groups remained significantly higher over control (never starved) while the same was found to be lower in 48 and 60 hrs starvation individuals. The RNA concentration remained significantly higher in all the treated lots. However, DNA content was not found to be significantly altered in hatched larvae after exposure to feeding stress. Protein, RNA and DNA concentration of 24 hrs old eggs produced by all the starved groups of Nistari, which had deceived two consecutive starvation during parental generations, showed higher concentrations of these biomolecules over control. Hence, starvation induced alterations in protein and nucleic acids in eggs and hatched Iarvae are indicative of a preparatory phase adopted by the insect to acclimatise itself and its progeny to stress situations.
Chaudhuri, S,Pandey, N K,Saini, Shrikant,Budhani, R C IOP Pub 2010 Journal of Physics, Condensed Matter Vol.22 No.27
<P>A dramatic drop of ≈5 orders of magnitude in the resistance (<I>R</I>) of La<SUB>0.175</SUB>Pr<SUB>0.45</SUB>Ca<SUB>0.375</SUB>MnO<SUB>3</SUB> epitaxial films upon exposure to optical photons derived from both continuous and pulsed lasers, as well as broad-band sources at temperatures (<I>T</I>) < 30 K is reported. The strength of change is a sensitive function of both the incident photon flux and temperature. Under isothermal conditions the photo-generated low resistance state persists eternally after removal of light. This non-equilibrium state is metallic, as revealed by the positive d<I>R</I>/d<I>T</I> for <I>T</I> ≤ <I>T</I><SUB><I>p</I></SUB> (≈120 K). This electrically conducting state is presumably ferromagnetic as <I>T</I><SUB><I>p</I></SUB> coincides with the temperature where a weak ferromagnetism sets in on cooling the insulating film from room temperature. To rule out the possibility of photon-induced local heating of the sample as a mechanism of the observed effects, photo-illumination experiments were performed under identical conditions on thin films of two non-charge-ordered manganites deposited on substrates of similar thermal conductivity. Our model for the observed transition encompasses a global charge-ordered state in which ferromagnetic metallic clusters of fraction <I>p</I> much less than the critical fraction <I>p</I><SUB>c</SUB> for percolation exists at low temperatures. Photo-induced melting of the charge-ordered state increases this fraction beyond <I>p</I><SUB>c</SUB> in a cumulative manner as successive pulses of light fall on the sample. </P>
Krishnan, N.,Chaudhuri, A.,Sengupta, A.K.,Chandra, A.K.,Sen, S.K.,Saratchandra, B. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2
The influence of baculovirus Bombyx mori Nuclear Polyhedrosis virus (BmNPV) infection on intermediary metabolic pathways in silkworm Bombyx mori L. was investigated. Studies revealed that NADP-linked malate dehydrogenase activity in hemolymph of infected silkworms at 96 hrs post infection (p.i.) with visible symptoms of infection was enhanced in comparison to healthy larvae of the same age. Also, NADP-dependent MDH activity was significantly lower in fat body cytosol of infected larvae at 96 hrs p.i. when compared to healthy larvae. Similarly, some biometabolic parameters like growth, protein content and cholesterol titer were observed to be influenced by baculovirus infection. While the growth of infected larvae was significantly retardedi protein content was also drastically reduced in both hemolymph and fat body tissues. Cholesterol titers however, was enhanced in infected larvae. The results observed herein point to a significant change in the normal biochemical and biometabolic pathways required for growth and development following BmNPV infection.
Saha, A. K.,Chaudhuri, A.,Krishnan, N.,Sengupta, A. K.,Shamsuddin, M.,Sen, S. K.,Saratchandra, B. 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.1
One indigenous polyvoltine strain (Nistari) and two bivoltine strains viz. P5, and NB18, along with one bivoltine hybrid (P5 X NB18) were subjected for studies on the differences on some physiologically important biomolecules like protein, nucleic acids and cholesterol. Ovarian protein and RNA content remained significantly high in the bivoltine races and their hybrid over multivoltine breed, while, DNA and cholesterol content remained significantly low in all the breeds and the hybrid as compared to Nistari strain. However, the ovarian weight was higher in both the bivoltine breeds and their hybrid than that of Nistnri. Higher ovarian weight together with more protein and RNA concentrations reflect the preparatory phase for production of diapausing eggs by the bivoltines. This is indicated by the production of more number of eggs by the bivoltine silkmoths and their hybrid. The variations in biochemical parameters studied herein, thus establish a distinct difference in the overt reproductive physioiogy between multivoltine and bivoltine silkworms.
Hamdani, Nissar Hussain,Qadri, Sumyra Khurshid,Aggarwalla, Ramesh,Bhartia, Vishnu Kumar,Chaudhuri, Sumit,Debakshi, Sanjay,Baig, Sarfaraz Jalil,Pal, N.K. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Gallbladder carcinoma (GBC) is the commonest cancer of the biliary tree and the most frequent cause of death from biliary malignancies. The incidence of GBC shows prominent geographic, age, race, and gender-related differences and is 4-7 times higher in patients with gallstones. This prompted us to study the clinicopathological aspects of the disease and the incidence of gallstones in gallbladder carcinoma patients, in this part of India. In this, combined retrospective (Jan 2004-March 2010) and prospective study (April 2010-Dec 2011) of eight years, 198 patients of gallbladder carcinoma (50 males and 148 females), (range 28-82 years; mean 55 years) were studied. Most of the patients were poor and presented with abdominal pain and mass, with abnormal lab parameters. Gallstones were present in 86% of patients. Surgical exploration was performed in 130, with gallbladder resection in 60 (including 7 incidental GBC). Adenocarcinoma (87.7%) was the commonest histological type. The study indicates that GBC is common in our scenario. It is a disease of elderly females, has a strong association with gallstones and every cholecystectomy specimen should be examined histopathologically.