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정영표,이동렬,손용,김태요,윤재승,송윤강,김명선,박래길 圓光大學校 醫科學硏究所 1999 圓光醫科學 Vol.15 No.2
Background: The effect of opioids on nitric oxide (NO)- and peroxynitrite-induced neuronal cell death is largely unknown. In the present study, we examined the effect of morphine on NO- and peroxynitrite-induced cell death using a human neuroblastoma SH-SY5Y cell line, which abundantly expresses μ, δ, k-opioid receptors. Methods: The cultured cells were pretreated with morphine and exposed to 3-morpholinosydnonimine (SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay ana crystal violet staining. Exposure of the cells to SIN-1 for 24 hours induced apoptotic cell death, as evaluated by the occurrence of morphological nuclear changes characteristics of apoptosis using 4', 6-diamidino-2-phenylindole (DAPI) and measurement of pro-apoptotic protease, caspase-3, activity. Results: Pretreatment of SH-SY5Y with morphine, significantly inhibited the apoptotic cell death in a dose-dependent manner. Morphine also inhibited SIN-1-induced proapoptotic protease, caspase-3, activity in a dose-dependent manner. However, naloxone (20 μM) hardly antagonized the effect of morphine in SIN-1-induced cell death. The selective ligands for opioid receptor subtypes, [D-Ala^2, N-Me-Phe^4, Gly-ol^5]enkephalin (DAMGO, μ-opioid receptor agonist), [D-Pen^2.5]enkephalin (DPDPE, δ-opioid receptor agonist) and U-69593 (k-opioid receptor agonist) at the concentration of 10 μM did not prevent the cell death induced by SIN-1. PI3-kinase inhibitors, Wortmannin and LY294002, did not inhibit the action of morphine on apoptotic cell death. The neuroblastoma cells treated with morphine significantly elevated glutathione levels (GSH). Conclusions: The present study showed that morphine protected human neuroblastoma cell line, SH-SY45Y, from the peroxynitrite-induced apoptotic cell death through elevated GSH levels. However, it is suggested that the elevation of GSH by morphine is not via the activation of opioid receptors and/or PI3-kinase pathway but via other unknown mechanism.
Ginseng for managing menopause symptoms
Myung-Sunny Kim,Hyun-Ja Lim,Hye Jeong Yang,Myeong Soo Lee,Byung-Cheul Shin,Edzard Ernst 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.1
The aim of this review was to assess the effectiveness of ginseng as a treatment option for managing menopause symptoms. We searched the literature using 11 databases from their inception to 26 September 2012 and included all randomised clinical trials (RCTs) that compared any type of ginseng to a placebo controls in postmenopausal women. The methodological quality of all studies was assessed using a Cochrane risk of bias tool. Four RCTs met our inclusion criteria. Most RCTs had high risk of bias. One RCT showed that Korean red ginseng (KRG) significantly improved sexual arousal and global health compared with placebo. Another RCT reported the superiority of KRG over placebo for treating menopause symptoms on Kupperman’s index and menopausal rating score. The third RCT failed to show a significant effect of KRG on hot flash frequency compared to placebo. The fourth RCT found beneficial effects of ginseng compared to placebo on depression and well-being. In conclusion, the evidence on ginseng as an effective treatment for managing menopause symptoms is limited. Most of the RCTs are burdened with a high risk of bias. Thus firm conclusions cannot be drawn. Rigorous studies seem warranted.
김명선 ( Myung Sunny Kim ),김태영 ( Tae Young Kim ),박래길 ( Rae Kil Park ) 대한뇌종양학회 2002 대한뇌종양학회지 Vol.1 No.1
Apoptosis is a complex process that removes aging or damaged cells from the body and occurs in a wide variety of organisms. Malignant gliomas are well known for their intrinsic heterogeneity that contributes to tumour recurrence, regardless current aggressive multimodality of therapy. New approaches of non-surgical intervention are formulated, according to specific characteristics of glioma biology including angiogenesis, invasion, DNA-repair mechanisms, cell cycle regulation and apoptosis. Specific proapoptotic approaches may overcome many of the obvious obstacles to achieve a satisfactory management of brain tumors. Various therapeutic approaches toward successful eradication of glioma by an induction of apoptosis have been developed, however these approaches may be hampered frequently by anti-apoptotic mechanism. One promising experimental strategy in treatment of glioma is to activate apoptosis pathway within tumor cells, which will employ the death ligands such as CD95(Fas/Apo1) ligand or Apo2 ligand(TRAIL), cytosolic protease, growth factor-related signal molecules, and intracelluar organelles(mitochondria and golgi complex), etc. In this review, we described the representative apoptotic pathways including Bcl-2, caspase, death receptor, and tumor suppressor gene to suggest their possibilities of adaption in comprehensive treatment to overcome chemoresistance or radioresistance of human brain tumor cells.
Kim, Myung-Sunny,Kim, Soon-Hee,Park, Su-Jin,Sung, Mi Jung,Park, Jaeho,Hwang, Jin-Taek,Yang, Hye Jeong,Kim, Sunmi,Seo, Daebang,Shin, Song Seok,Hur, Haeng Jeon Elsevier 2017 Journal of Functional Foods Vol.35 No.-
<P><B>Abstract</B></P> <P>This study investigated the effect and the underlying mechanism of ginseng berry (GB) in a mouse model of type 2 diabetes, supplied with 0.05% or 0.1% dietary GB for 12weeks. GB significantly improved hyperglycemia and insulin resistance, as demonstrated by the blood glucose and insulin levels, HOMA-IR, and GTT. Moreover, the expression of gluconeogenic genes such as PEPCK and G6Pase and the hepatic metabolites involved in the pathway of gluconeogenesis, such as glucose-6-phosphate and dihydroxyacetone phosphate, were significantly reduced by GB. Hepatic steatosis was also significantly ameliorated; TG content and expression of lipogenic enzymes and transcriptional factors such as FAS, ACC, and SREBP1 were reduced by GB. Simultaneously, AMPK phosphorylation was increased both in fatty liver and in lipid-accumulated HepG2 cells by GB. In conclusion, GB improved hyperglycemia by downregulating hepatic glucose production and hepatic steatosis, and AMPK appeared to be an important regulator of these effects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GB significantly improves obesity-induced hyperglycemia and insulin resistance. </LI> <LI> Anti-diabetic effects of ginseng berry is mediated by downregulating hepatic glucose production and hepatic steatosis. </LI> <LI> AMPK may be an important contributing regulator in the GB-mediated suppression of hepatic glucose production. </LI> </UL> </P>
Protective effects of Korean herbal remedy against oxidative stress in cardiomyocytes
Kim, Myung-Sunny,Kwon, Dae Young,Cho, Hye-Jin,Lee, Myeong Soo John Wiley Sons, Ltd. 2006 Phytotherapy research Vol.20 No.3
<P>Ondamtanggagambang (ODG) has been used as a prescription for psychological anxiety and depression in Korean medicine. In this study, we found that ODG have protective effects against oxidative stress in cardiomyocytes. Pretreatment with ODG extract prevented H<SUB>2</SUB>O<SUB>2</SUB> and ZnCl<SUB>2</SUB>-induced cell damage in H9c2 cardiomyocytes, whereas simultaneous treatment of ODG extract did not. The protective effect of ODG extract on oxidative stress-induced damage was suppressed significantly by heme oxygenase (HO) inhibitors, zinc protoporphyrin-IX (ZnPP-IX, p < 0.01) and tin protoporphyrin-IX (SnPP-IX, p < 0.01) in H9c2 cells. ODG stimulation of cells strongly induced the expression of HO-1 protein. Taken together, it is suggested that ODG-induced expression of HO-1 may have a beneficial role in cardiomyocytes under oxidative stress. Copyright © 2006 John Wiley & Sons, Ltd.</P>
Ginseng for managing menopause symptoms: a systematic review of randomized clinical trials
Kim, Myung-Sunny,Lim, Hyun-Ja,Yang, Hye Jeong,Lee, Myeong Soo,Shin, Byung-Cheul,Ernst, Edzard The Korean Society of Ginseng 2013 Journal of Ginseng Research Vol.37 No.1
The aim of this review was to assess the effectiveness of ginseng as a treatment option for managing menopause symptoms. We searched the literature using ll databases from their inception to 26 September 2012 and included all randomised clinical trials (RCTs) that compared any type of ginseng to a placebo controls in postmenopausal women. The methodological quality of all studies was assessed using a Cochrane risk of bias tool. Four RCTs met our inclusion criteria. Most RCTs had high risk of bias. One RCT showed that Korean red ginseng (KRG) significantly improved sexual arousal and global health compared with placebo. Another RCT reported the superiority of KRG over placebo for treating menopause symptoms on Kupperman's index and menopausal rating score. The third RCT failed to show a significant effect of KRG on hot flash frequency compared to placebo. The fourth RCT found beneficial effects of ginseng compared to placebo on depression and well-being. In conclusion, the evidence on ginseng as an effective treatment for managing menopause symptoms is limited. Most of the RCTs are burdened with a high risk of bias. Thus firm conclusions cannot be drawn. Rigorous studies seem warranted.