http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Association analysis between genomic region of isoflavone reductase gene and maturity in soybean
Solmoe Lee,Myounghai Kwak,Kyujung Van,Moon Young Kim,Suk-Ha Lee 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
The control of earliness has an agronomic importance since it reduces growing and harvesting time. Earliness is controlled by multiple genes in multiple pathways and influenced by the environment. In Arabidopsis thaliana, many earliness related genes were identified. Among them, Arabidopsis Frigida (FRI) gene confers late flowering phenotype, which is reversed to earliness by vernalization. Blast search using FRI against soybean EST database at TIGR identified Isoflavone reductase-like gene (TC217830). Fifty seven SNPs were identified in a total of 4,242 bp lengths in genomic region of Isoflavone reductase-like from 62 soybean genotypes (31 early maturity group and 31 late maturity group). From the obtained sequences, we identified 6 haplotypes of Isoflavone reductase-like gene. Among them, three haplotypes showed a significant association with maturity, suggesting that Isoflavone reductase-like gene is tightly linked to flowering time or actual gene it-self. Thus, to delimit a putative genomic region for maturity and flowering time, SSR markers near Isoflavone reductase-like gene were designed and analyzed for their genetic diversity, assuming that highly selected regions might posses lower genetic diversity. Through these experiments, the region related to maturity and flowering was delimited to nearby ac_satc_4 in scaffold 16.
Jungmin Ha,Myounghai Kwak,Kyujung Van,Moon Young Kim,Suk-Ha Lee 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
MADS-box genes encode a family of transcription factors which involve in diverse developmental processes in flowering plants. Because flowering time determines the timing of transition from vegetative to reproductive stage and time to harvest, it would be a significant trait not only to plant it-self but also to breeders. The sequences and gene structures of Arabidopsis MADS-box genes are conserved in model legumes. However, complex genome structure, in soybean, makes it difficult to identify actual genes related to flowering and maturity, although QTL researches have been generally conducted. Therefore, we hypothesized that putative MADS-box genes around the flowering time and maturity QTLs would be candidate genes for those loci. In this study, after surveying 84 QTLs highly associated with maturity and flowering, the QTLs were selected if they were located near 473 putative MADS-box genes. Finally, we found the highly associated 16 SNPs at non-coding region of the putative MADS-box gene around the QTL in 28 late maturity cultivars and 28 early maturity cultivars. Furthermore, by comparing genetic diversity in the cultivated soybeans of late and early maturity groups as well as 20 wild soybeans, selection pattern during domestication was predicted.
Ganghwal is a new species, Angelica reflexa
Lee, Byoung Yoon,Kwak, Myounghai,Han, Jeong Eun,Jung, Eun-Hee,Nam, Gi-Heum The National Institute of Biological Resources 2013 Journal of species research Vol.2 No.2
A new species of Apiaceae, Angelica reflexa from Daeamsan, Gangwon-do province, Korea is described and illustrated. This new species is closely related to A. genuflexa Nutt. ex Torr. & A. Gray, having such characters as leaf rachis and petiolules refracted strongly, but distinguished from the latter by its regularly serrated leaf margins, glabrous on abaxial leaf veins, and two vittae on the commissural face of the mericarp.
Chang-Uk Park,Takeshi Ogura,Myounghai Kwak,Hee-Young Chae 한국조류학회II 2013 한국조류학회지 Vol.20 No.1
This study confirmed the presence of a new breeding site of the crested murrelet (Synthliboramphus wumizusume) on the uninhabited Baek-do Islands, Korea, which have been regarded as a potential breeding place. We visited the Baek-do Islands thrice from 2011 to 2012. During the visit in August 2011, we found some dead crested murrelets on Sangbaek-do Islet. During the visit in April 2012, we observed 23 individuals near Sangbaek-do and Habaek-do islets by using night spotlight survey and breeding nest survey. We also found 2 unsuccessful nests on the Geobuk-bawi Rock of Sangbaek-do islet, of which one was confirmed as that of a crested murrelet nest. Therefore, we conclude that crested murrelets breed on the Baek-do Islands. The carcasses and eggshells collected from the island during the surveys indicate that the Norway rat (Rattus norvegicus) is a major threat to the successful breeding of crested murrelets. We recommend that long-term monitoring and supplementary surveys are required to obtain a baseline understanding of the population trends of crested murrelets, as well as to identify potential threats and delineate mitigation methods.
Won, Hyosig,Yun, Young-Eun,Kwak, Myounghai,Han, Jeong Eun The National Institute of Biological Resources 2012 Journal of species research Vol.1 No.2
To assess the genetic diversity of Aconitum coreanum (Ranunculaceae) populations in Korea, we have amplified and sequenced eight organellar marker regions, and developed and analyzed microsatellite markers. No sequence variation was detected from the eight organellar markers. Ten microsatellites were developed using Next Generation Sequencing and two microsatellite markers, AK_CA03 and AK_CT07, were identified polymorphic and applied for 143 individuals of twelve A. coreanum populations. Four and five alleles were detected for the two microsatellite loci, respectively, and number of migrants ($N_m$) was estimated as 1.12586. Two microsatellite marker loci showed $F_{ST}$ of 0.205 and 0.275, respectively. The heterozygosity deficit, low level of among-population differentiation, small size of gene flow, and lack of sequence variation of the organellar markers suggest that A. coreanum is reproductively isolated from other Aconitum species and there has been continuous gene flow among the populations of A. coreanum or it has dispersed relatively recently after speciation. Though population pairwise $F_{ST}$'s presented significant geographic structure, further sampling and study will be necessary to confirm this.
Han, Jeong Eun,Choi, Byoung-Hee,Kwak, Myounghai The National Institute of Biological Resources 2018 Journal of species research Vol.7 No.4
Population genetic assessment is essential for the conservation and management of endangered and rare plants. Neofinetia falcata is endangered epiphyte orchid and protected by law in Korea. In Korea, this species is only found on islands in the South Sea of Korea (including Jeju-do) and the southern coast of the Korean Peninsula. We developed nine microsatellite makers to assess the genetic diversity and population genetic structure of three populations of N. falcata. The genetic diversity at the species level was low, which can be attributed to inbreeding or fragmentation into small, isolated populations. A recent bottleneck was detected in one population, likely due to overcollection. N. falcata exhibited moderated levels of differentiation among populations, with the three populations were divided into two clusters based on genetic structure. The genetic diversity and structure of N. falcata are affected by restricted gene flow by pollen or seeds due to isolation and geographic distance. Strategies for in situ and ex situ conservation of this species are been proposed based on the results of our study.
Kang, Jong‐,Soo,Lee, Byoungyoon,Kwak, Myounghai John Wiley Sons Australia, Ltd 2017 Plant species biology Vol.32 No.4
<P>We isolated and characterized microsatellite loci in <I>Viola mirabilis</I> (Violaceae), an endangered species from South Korea. Twenty‐three polymorphic microsatellite loci were developed and tested in Korean, Chinese and Japanese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within the three populations were 0.000–0.625 and 0.469–0.695, respectively. A total of six loci in the Korean population, one locus in the Chinese population and seven loci in the Japanese population deviated from Hardy–Weinberg equilibrium. We expect that these newly developed microsatellite markers will contribute to understanding the phylogeography and population genetics of <I>V. mirabilis</I>, which will aid in developing conservation strategies for this species.</P>
Kang, Jong-Soo,Zhang, Xianchun,Kwak, Myounghai Springer-Verlag 2017 Plant molecular biology reporter Vol.35 No.1
<P>We isolated and characterized microsatellite loci in Viola websteri (Violaceae), an endangered species from Korea and endemic to Northeast Asia. A total of 27 microsatellite loci were developed and tested in Korean and Chinese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within two populations were 0.000 to 1.000 and 0.080 to 0.816, respectively. Korean and Chinese populations were clearly distinguished by the private alleles from 16 loci. A total of 21 loci out of the 27 developed loci were successfully cross-amplified in 39 other Viola species. We believe that these microsatellite loci will be useful for future studies on genetic diversity and population structure of V. websteri, as well as other Viola species.</P>