단백질분해효소를 생성분비하는 호알칼리성 Coryneform bacterium TU-19의 분리 및 동정

최명철,양재섭,강선철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

토양으로부터 알칼리성 단백질분해효소를 생성분비하는 호알칼리성 미생물을 분리하였다. 이 균의 형태적 특징은 Gram 양성이었으며, 포자를 형성하지 않고, 운동성이 없으며, 불규칙적인 간균의 형태를 하고 있는 호기성 미생물이었다. 또한 기타 다른 형태적, 생리생화확적 특징과 종합적으로 비교해 볼 때 이 균주는 Coryneform 세균과 매우 유사하였다. 따라서 본 분리균주는 Coryneform bacterium TU-19로 잠정적으로 동정하였다. 그러나 화학적 분석 결과 및 부분적인 생리생화적 특징들에 있어서는 Coryneform bacteria의 어느 종과도 정확히 일치하지 않았다. An alkalophilic bacterium producing alkaline protease(s) was isolated from soil. It was a Gram-positive, non-sporulating, immotile, irregular rod, strictly aerobic, and weak acid-forming bacterium. The morphological, physiological, and biochemical characteristics of the isolate resembled those of the Coryneform bacteria. However, there was not any species within this genera to which microorganism can be closely matched. Therefore, it is provisionally identified as a Coryneform bacterium TU-19.

二家花樹木에 있어서 雌雄株間의 形態的 差異에 관한 硏究

任炯卓,崔明燮 건국대학교 1982 論文集 Vol.15 No.1

This work was investigated on the morphological differences between male and female of the dioecious planes. Morphological characters, mainly in shape of leaves of each male and female of some dioecious treed, are observed by naked eyes and stereoscopes. The results are following 1.In Populus tomentiglandulosa, the ratio of leaf size between a twig arid 2-year old branch of female tree is much greater than that of male. 2.In Populus euramericana, the color of petiole and a young leaf of female are reddish, but that of male is reddish and green. 3.In Slix pseudo-lasiogyne, the number of teeth of male is above 100 but that of female is below 100. Especially there are considerable instability of sexual condition in some male and female treed. 4.As for the color of upperside of petiole, the scarlet vestige of male is more ditinct than that of female in Salix graciliglans. 5.In Slix purpurea var. japonica, male has 1 or 2 lenticels below the basementof petiol but female has 3. And there are more than 5 lenticels on internode of female on the other hand male has leas than 3. 6.In matured Ginkgo biloba, the spur of male developes more vigorously than that of female and as for the bud of slur apex, male's is twice as large as female's.

활성탄의 급여가 젖소 송아지의 성장 및 배설물의 Ammonia 함량에 미치는 영향

유선일,박덕섭,명윤아,이인덕,남명수,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was carried out to investigate the influences of feed containing activated charcoal on the performances of calves The daily weight gain, feed intake, feed requirement and the content of ammonia in the excreta were measured. The experiment was conducted with 10 male calves for 30 days. Each were equally divided with treatment group and control group and for the treatment group's animal fed diets containing 1.0% activated charcoal. The daily weight gain of calves were decreased by 1.92% when they were fed activated charcoal(1.02㎏/day) compared to that of control calves(1.04㎏/day) and the daily feed intake of treatment group(2.86㎏/day) was 10.06% lower comparing to the control group(3.18㎏/day). In case of feed requirement, the result showed 8.2% lower in the treatment group(2.80㎏) than control group(3.06㎏). The content of ammonia in excreta was significantly lower for treatment group(0.33ppm) than control group (2.67ppm)(p<0.05), which showed more improvement for reducing ammonia content in the treatment group with 87.6%(p<0.05). 본 연구는 사료에 활성탄을 첨가했을 때 송아지의 일당 증체량, 사료 섭취량, 사료 요구율 및 배설물의 ammonia 함량에 미치는 영향을 규명하기위해 실시하였다. 홀스타인 수송아지 10두를 30일 동안 대조구, 시험구(활성탄 1%첨가)로 각5두 공시하여 시험하였으며, 실험 결과를 요약하면 다음과 같다. 송아지의 일당 증체량은 대조구(1.04kg/일)에 비하여 활성탄을 급여했을 때(l.02kg/일) 1.92% 감소하였고, 일당 사료 섭취량은 시험구(2.86kg/일)가 대조구(3.18kg/일)에 비해서 10.06% 낮았으나, 사료 요구율은 대조구(3.06kg/kg증체)에 비해 시험구(2.80kg/kg증체)에서 8.2% 낮은 결과를 나타내었다. 송아지 배설물의 ammonia 함량은 대조구의 2.67ppm에 비하여 시험구가 0.33ppm으로 87.6% 감소되는 개선효과를 보였다(p<0.05).

柔道技術 중 허벅다리걸기의 運動學的 特性

신성휴,손명성,김원섭,최승필 한국운동역학회 1998 한국운동역학회지 Vol.8 No.2

This study has been aimed to analyze UCHIMATE technique of Judo gymnastically. The following are the results and the proposition obtained through an image analysis method 3 men half heavy weight champions of the national Judo standing team(85-95kg) were used as subjects for the study. 1. CONCLUSION The following are the conclusion obtained through the discussion of analysis results of the Uchimata technique using 3 men, half heavy weight(85-95kg) champions of the National standing Judo team. 1) The time performing total technique were 1.48-seconds which was performinglonger than the light heavy weight class of 1.28-seconds by 0.20-seconds, this showed that the heavy weight class players demonstrated the technique later than the light heavy weight class palyers. 2) The average length of moving 1-step leg for attacking was 24.27cm, and 2-step leg moving was 92.87cm on average. Therefore, the shorter the moving distance, the more the technique performing hour could be shortened. 3) In the hanging phase, the moving displacement of the center of gravity of the body weight showed in horizontal, vertical, right and left direction 28.97cm, 21.59cm and 13.57cm respectively. Each of which were longer than the phase of KUZUSHI and TSUKURI. Such results show that the center of gravity of the body in move lift the opponent. This reducing the displacement of body weight from the center toward the right and left direction in the phase of KAKE is considered to be effective in demonstrationg the technique. 4)The central speed of the body was 0.8cm per second on average and the average vertical speed was 0.74cm per second. The speed of right and left movement was 0.51cm per second on average which showed the horizontal speed was the fastest while it is preferred that the vertical speed should be faster than that of the horizontal. 5) Angle of the Trunk showed 98.13 ˚ on average in the hanging phase and the larger the trunk angle, the shorter the needed time to demonstrate the technique, thus making the throw of the opponent over shoulder more advantageous. 2. PROPOSTION The following propositions are suggested through this analysis for further study : 1) It is to be considered that the kinetics in hanging the thigh and the degree of every segment of the body be studied in a more detailed fashion. 2) A study on the technique of thigh hanging in an actual game situation (rather than during mere exercises) is necessary. 3) It is necessary to study the TORI and UKE techniques together. 4) The study of each weight class ought to be performed.

Lactobacillus casei AHU 1055 및 Lactobacillus acidophilus JAM 1043의 凍結乾燥와 生存率에 關한 硏究

金昌漢,金明浩,朴芝賢,李明燮,朴相瑨 건국대학교 동물자원연구센터 1992 動物資源硏究誌 Vol.17 No.-

In order to find the most suitable conditions of freeze-drying for the long term preservation of lacto-bacillus casei AHU 1055 and Lactobacillus acidophilus JAM 1043, the growth phase, the cryoprotection medium and inoculum size have been studied. The results obtained in this study are summerized as follows : 1. L. casei AHU 1055 and L.acidophilus JAM 1043 reached to stationary phase after incubating at 37˚C for 16hrs and 12hrs in MRS broth, respectively. 2. The highest viability of each strains was obtained in 11% skim-milk for L. casei AHU 1055 and 9% skim-milk for L. acidophilus JAM 1043 as basic cryoprotection medium. 3. The complex medium contained 11% skim milk powder, 2.5% arginine and 3% glucose was the best suspending midium for freeze-drying of L. casei AHU 1055, and the medium contained 9% skim milk powder, 7% glycerol, 9% lactose, 0.5% arginine was the best suspending medium for freeze-drying of L. acidophilus JAM 1043. 4. The strong positive correlation was found between inoculum size and the survival rate in both strains. 5. When the tested strains were cultured and freeze-dried under the best optimal conditions, the survival rates of freeze- dried L. casei AHU 1055 and L. acidophilus JAM 1043 were 85.8% and 84.0%, respectively.

Versatile approaches to tune a nanocolumnar structure for optimized electrical properties of In₂O₃ based gas sensor

Han, Soo Deok,Noh, Myoung-Sub,Kim, Sangtae,Shim, Young-Seok,Song, Young Geuon,Lee, Kwangjae,Lee, Hae Ryung,Nahm, Sahn,Yoon, Seok-Jin,Kim, Jin-Sang,Kang, Chong-Yun Elsevier 2017 Sensors and actuators. B, Chemical Vol.248 No.-

<P><B>Abstract</B></P> <P>In this study, we demonstrate tuning of electrical properties and sensing responses of In<SUB>2</SUB>O<SUB>3</SUB> nanocolumnar structure via varying glancing angle (GLAD) deposition conditions by e-beam evaporator. The varied deposition conditions include glancing angle, vacuum level and deposition rate. The electrical property of In<SUB>2</SUB>O<SUB>3</SUB> nanostructured thin films, demonstrated by the base resistance, change up to 3 orders of magnitude from 110Ω to 103104Ω depending on the porosity of nanocolumnar structure and oxygen vacancy concentration. This variation in electrical property transfers to the tuning of gas sensing response, and we achieve tuning the same material (In<SUB>2</SUB>O<SUB>3</SUB>) based gas sensors to better perform for specific type of gases (either oxidizing or reducing). The highest responses achieved in this work reached up to 176 for oxidizing gases (5ppm NO<SUB>2</SUB>, R<SUB>gas</SUB>/R<SUB>air</SUB>) and 929 for reducing gases (50ppmC<SUB>2</SUB>H<SUB>5</SUB>OH, R<SUB>air</SUB>/R<SUB>gas</SUB>). Therefore, we demonstrate that gas sensors can be optimized for specific type of target gases with the same material, via simple control of deposition conditions. Along with the high reproduciblility and sensitivity, this puts the nanocolumnar thin film based gas sensors by GLAD with huge potential for further miniaturization and mass production, suitable for the upcoming IoT era.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanocolumnar In<SUB>2</SUB>O<SUB>3</SUB> thin film based gas sensor is fabricated by glancing angle deposition (GLAD). </LI> <LI> Electrical properties of In<SUB>2</SUB>O<SUB>3</SUB> can be optimized for specific target gases, tuning simple deposition conditions of GLAD. </LI> <LI> In<SUB>2</SUB>O<SUB>3</SUB> sensors have excellent performances with high repeatability, recovery, and extremely low detection limit. </LI> </UL> </P>

Downsizing gas sensors based on semiconducting metal oxide: Effects of electrodes on gas sensing properties

Song, Young Geun,Shim, Young-Seok,Kim, Sangtae,Han, Soo Deok,Moon, Hi Gyu,Noh, Myoung Sub,Lee, Kwangjae,Lee, Hae Ryong,Kim, Jin-Sang,Ju, Byeong-Kwon,Kang, Chong-Yun Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.248 No.-

<P><B>Abstract</B></P> <P>We report the highly sensitive and selective downsized gas sensors for the IoT application. Sensing areas of Pt-interdigitated electrodes (IDEs) were varied to investigate the relation between sensing materials and electrodes. In<SUB>2</SUB>O<SUB>3</SUB> nanocolumns were deposited on the pre-patterned Pt IDEs using glancing angle deposition (GLAD). The effect of the interface resistance between electrodes and sensing materials, and the intergrain resistance between nanocolumns is analyzed by linear regression at different sensing area and incident angle of GLAD. In<SUB>2</SUB>O<SUB>3</SUB> (angle: 85°, sensing area: 0.3mm×0.3mm) nanocolumns with double Schottky barriers show the highest response and selectivity with fast response time of 10s to VOCs among the samples fabricated in this study. The analysis reveals that the intrinsic response of In<SUB>2</SUB>O<SUB>3</SUB> (angle: 85°, sensing area: 0.3mm×0.3mm) nanocolumns are dominantly affected by intergrain resistance, resulting in a high response. Our demonstration for the fundamental aspect of downsizing gas sensor makes an important contribution to the chemical sensor field with broad interest.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The sensing areas are varied to investigate the relation between sensing materials and electrodes. </LI> <LI> The effect of the interface resistance and the intergrain resistance are analyzed by linear regression. </LI> <LI> The performance of the downsizing gas sensor is greatly influenced by the interface resistance. </LI> </UL> </P>

Analysis on the phase transition behavior of Cu base bulk metallic glass by electrical resistivity measurement

Ji, Young Su,Chung, Sung Jae,Ok, Myoung-Ryul,Hong, Kyung Tae,Suh, Jin-Yoo,Byeon, Jai Won,Yoon, Jin-Kook,Lee, Kyung Hwan,Lee, Kyung Sub Elsevier 2007 Materials science & engineering. properties, micro Vol.449 No.-

<P><B>Abstract</B></P><P>The crystallization behavior of Cu<SUB>43</SUB>Zr<SUB>43</SUB>Al<SUB>7</SUB>Ag<SUB>7</SUB> (numbers indicate at.%) bulk metallic glass was investigated using the isothermal electrical resistivity measurements at 450°C in the supercooled liquid region. The crystallization process is a single step phase transformation. To analyze the electrical resistivity reduction, microstructure evolutions were analyzed using differential scanning calorimetry, X-ray diffraction, transmission electron microscopy and small-angle X-ray scattering. The Avrami parameter of the electrical resistivity reduction step was 1.73, indicating that the crystallization process is a diffusion-controlled growth of intermetallic compounds with decreasing nucleation rate.</P>

저온 초음파 추출에 의한 Spirulina maxima 면역활성 증진

오성호(Sung Ho Oh),한재건(Jae Gun Han),하지혜(Ji Hye Ha),김영(Young Kim),정명훈(Myoung Hoon Jeong),김승섭(Seong Sub Kim),정향숙(Hyang Suk Jeong),최근표(Geun Pyo Choi),박욱연(Uk Yeon Park),강도형(Do Hyung Kang),이현용(Hyeon Yong Lee) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.3

본 실험은 해수에서 배양된 Spirulina maxima를 100℃와 초음파 병행 60℃ 물 추출물, 80℃ EtOH 추출물을 이용하여 실험을 수행하였다. AOAC법에 의한 일반 조성 및 무기질, 아미노산 조성 분석결과 조 단백질과 무기질 중 나트륨이 각각 56, 60%의 비교적 높은 비율을 차지했으며, 다양한 균주의 필수 아미노산을 포함하고 있었다. 그중 피부와 관련된 leucine의 함량이 9.83%로 상대적으로 풍부하여 S. maxima 피부면역 활성 증진에 효율적인 소재의 가능성을 확인했다. 본 연구에서는 피부 면역과 인체 면역과의 밀접한 상관성이 있으므로 본 연구에서는 T 세포와 B 세포의 생육증진 및 cytokine의 분비량 측정과 NK 세포의 활성 및 항염증 효과인 hyaluronidase 저해 활성 효과를 측정하였다. 먼저 정상세포(HEK293)에 대한 세포독성 결과 인체에 큰 독성을 나타내지 않는 것으로 나타난 S. maxima를 추출조건에 따른 면역증진 효과를 비교한 결과 60℃ 초음파 병행 물 추출물이 면역 B세포와 T세포에서 각각 11.3×10sub{4} cells/mL, 12.8×10sub{4} cells/mL으로 6일째 가장 높은 생육도를 보였다, 이러한 면역 세포들이 분비하는 cytokine(IL-6, TNF-α)의 분비량은 가장 높은 생육도를 보인 60℃ 초음파 병행 물 추출물을 첨가한 B세포, T세포의 경우 IL-6와 TNF-α는 유의적으로 증가된 분비량을 보이며, 면역 및 기능성 소재로서의 활성이 뛰어난 것으로 나타났다. B세포에 각 시료를 첨가하여 배양 후 그 배양액을 NK 세포에 첨가하였을 때, 6일 동안 생육도를 관찰하였는데 시료에 대한 생육도가 증가하며, 6일째 12.40×10sub{4} cells/mL를 나타내어 11.49×10sub{4} cells/mL를 나타낸 대조군에 비해 높은 활성을 나타내었으며, 이로써 활성물질을 포함하고 있음을 알 수 있다. 또한 항염증 효과의 hyaluronidase 저해 활성 효과에서 60℃ 초음파 병행 물 추출물이 1.0mg/mL 농도일 때 62.4%의 저해 활성을 보여 100℃ 이상의 고온 추출에서 야기되는 단백질이나 미립자 활성 물질의 파괴가 최소화되고 저온 초음파 추출 시 유용성분들의 용출량 증가와 새로운 물질들의 생산 혹은 활성변형에 의한 추출을 통해 인체면역과 피부면역 활성 증진에 활용이 가능한 효율적인 소재화가 가능한 것을 확인했다. 이로써, 본 연구 논문은 S. maxima의 물질 분리보다 피부면역관련 활성을 통한 소재로서의 탐색이 주목적이므로 향후 이 추출물의 분리, 동정을 통해 피부 면역 활성도에 따라 물질의 구조 분석에 관한 연구를 보이고자 한다. The marine microalga Spirulina maxima was extracted using water or ethanol at 100 or 80℃ and by ultrasonification in water at 60℃. The ultrasonification technique generated the highest yield (19.8%). To be therapeutically useful, the extraction should yield a product with low cytotoxicity and high immunity against skin infections. The cytotoxicity of all extracts (1.0 mg/mL) was below 25%. Moreover, the cytotoxicity of the extract generated by ultrasonification was 5%. Extracts prepared in the described manners could inhibit hyaluronidase activity by up to 40% compared to the control. Increased growth of human B, T and NK cells and an increase in cytokine secretion were observed, confirming the interrelationship between both human immune and skin immune activity. The extract prepared by ultrasonification increased the growth of human B, T and NK cells up to 10.3×10sub{4} cells/mL, 11.3×10sub{4} cells/mL and 19.1×10sub{4} cells/mL, respectively. The extract prepared by ultrasonification also greatly increased the secretion of both IL-6 and TNF-α. Moreover, it was estimated that protein, Na and leucine occupy a high ratio. Accordingly, this study has confirmed that extracts prepared as described have the potential to effectively increase skin immunity.

Coupling Performance Analysis of a Buried Meshed-Ground in a Multi-layered Structure

Myoung-Sub Joung,Jun-Seok Park,Hyeong-Seok Kim,Jae-Bong Lim,Hong-Goo Cho 대한전기학회 2004 KIEE International Transactions on Electrophysics Vol.4C No.6