흰쥐 재생간의 Leucine Aminopeptidase의 활성치

문교철,곽춘식,김여희 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

Changes in the activities of the followings have been studied over a period of 6 days after partial hepatectomy in rats: Plasma membrane, mitochondrial, nuclear and cytosolic leucine aminopeptidase and microsomal-particle bound aminopeptidase of the regenerating liver and serum leucine aminopeptidase. The activities of alkaliine phosphatase in the subcellular fractions were also measured. The activities of leucine aminopeptidase and alkaline phosphatase in serum markedly elevated from 12 hours to three days after partial hepatectomy in rats. The activity of plasma memberane-bound leucine aminopeptidase in the regenerating rat liver drastically increased during the first and the third days of the operation. And the activity of microsomal particle-bound aminopeptidase in the regenerating liver significantly increased between the second and the third days after operation. The activities of nuclear and cytosolic leucine aminopeptidase in the regenerating liver showed a substantial increase at the second day and from first to sixth day respectively after operation. However, no significant change in hepatic mitochondrial leucine aminopeptidase was noted throughout the experiments. The activities of plasma membrane, microsomal, mitochondrial, nuclear and cytosolic alkaline phosphatase in the regenerating liver markedly increased throughout the experiments.

흰쥐에게 내독소의 비경구 투여가 혈청 및 간의 Alkaline Phosphatase 및 5'-Nucleotidase 활성도 변동에 미치는 영향

문교철,곽춘식,주일 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

The activities of the cytosolic, microsomal and mitochondrial alkaline phosphatase(ALP) and 5'-nucleotidase(5'-NT) in the liver were measured in order to evaluate the cholestatic changes, one of the hepatic damages under the endotoxin administration. And the activities of these enzymes in the serum were also measured. For administration of endotoxin, a dose of 5mg of endotoxin(lipopolysaccharide E. coli 026: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3,8 and 24 hours of injection with endotoxin to measure the activities of the above enzymes in serum and their livers. Cyrtosolic and microsomal ALP and 5'-NT activities showed a significant increase between 3 and 8 hours after endotoxin administration. The activity of the mitochondrial ALP and 5'-NT in the liver showed no significant changes throughout the experiment. Serum ALP activity showed a increase without significace. But serum 5'-NT showed a significant increase at 8 hours after endotoxin administration. According to the results, it is suggested that cytosolic and microsomal ALP and 5'-NT activity is increased caused by increased biosynthesis and impairment of hepatic excretory function for these enzymes. And it is also suggested that increased activity of the serum ALP and 5'-NT is due to the reflux of these enzymes from the liver and due to leak into the blood through the damaged membrane of hepatocyte.

Effect of Melatonin on Hemolysis Induced by Cyclosporine

곽춘식,문교철,서성일 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

Effects of Intravenous Administration of Taurocholate on Hepatic Aryl Sulfotransferase Activity in Cholestatic Rats

곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The possible mechanisms of increased aryl sulfotransferase (AST) isozymes activities in cholestatic rat liver were studied. Hepatic AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ activities were determined from the experimental rats with common bile duct ligation (CBDL). The Michaelis-Menten constants in these hepatic enzymes were also measured. The activities of mitochondrial AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ, and microsomal AST-Ⅲ, Ⅳ as well as their Vmax values were found to be increased significantly in CBDL plus taurocholic acid (TCA) injected group than in the control group, such as CBDL alone groups. However, their Km values in the experimental groups did not vary. The results suggest that TCA stimulates biosynthesis of the AST in the liver.

Effect of Acute Ethanol Intoxication on Hepatic Rhodanese Activity in Rats with Extrahepatic Cholestasis

곽춘식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2

Liver and serum rhodanese activities were determined in acute ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD) to manifest the biochemical background of alcohol drinking hazard under the hepatobiliary disease. Liver cytosolic and microsomal rhodanese activities and these Vmax values in CBD ligated rats with acute ethanol intoxication were found to be decreased much more than that in CBD ligation alone. However, the difference of Km value on above hepatic enzyme was not found between the experimental groups. On the other hand, serum rhodanese activity in CBD ligated rats with acute ethanol intoxication was greater increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic rhodanese decreases and the serum rhodanese activity increases in cholestasis combined with acute ethanol intoxication. reflecting damage of aggravated hapatocytic membrane. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.

흰쥐 재생간의 Monoamine Oxidase 의 활성치

박은미,곽춘식,문교철,김여희 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

This study was intended to investigate the changes of hepatic mitochondrial and microsomal monoamine oxidase(MAO) A and B activities after 70%(median and left lateral lobes) partial hepatectomy in rats. The activities of mitochondrial MAO A and B in the regenerating liver significantly increased between the second and the third days of the operation. However, the microsomal MAO A activity in regenerating liver showed a marked decrease from two days after operation.

흰쥐 재생간의 Malate Dehydrogenase의 활성치

김여희,곽춘식,문교철 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

A study was made on the changes in the activities of the following during 6 post-perative days: Cytosolic and mitochondrial malate dehydrogenase of regenerating rat livers, and rat serum malate dehydrogenase after 70% (median and left lateral lobes) partial hepatectomy. The activities of lactate dehydrogenase and alanine aminotransferase in both serum and cytosol of regenerating rat liver were also measured. After parial hepatectomy in the rats, activity of serum malate dehydrogenase trmendously increased in in the span between the 12 hours and the second day. The activity of cytoslic malate dehydrogenase in the regenerating liver significantly increased from the first to the second day after partial hepatectomy. However, no significant changes in hepatic mitochondrial malate dehydrogenase was noted throughout the experiments. The activities of serum lactate dehydrogenase and alanine aminotransferase markedly elevated after partial hepatectomy. And activity of alanine aminotransferase significantly decreased in the hepatic cytosol after partial hepatectomy but hepatic cytosolic lactate dehydrogenase showed no change.

Catechol-O-Methyltransferase Activity in Cholestatic Rat's Liver Induced by Bile Duct Ligation

Mun, Kyo-Cheol Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.2

To investigate the cause of increased plasma catecholamine levels in liver disease, catechol-O-methyltransferase (COMT), which provides a major route of catabolism for circulating catecholamines, was studied under the cholestasis induced by mechanical biliary obstruction in rats. Monoamine oxidase (MAO) activity and the $K_m$ and $V_{max}$ values for both enzymes were also measured. Cytosolic, microsomal, and mitochondrial COMT activities in the cholestatic liver were significantly decreased throughout the experiment. Microsomal, and mitochondrial MAO activity in the cholestatic liver were also significantly decreased. Vmax values of COMT and MAO were lower. Serum COMT and MAO activities were detected after CBD ligation. These results indicate that plasma catecholamine levels are increased in liver disease due to decreased hepatic degradation of catecholamines by decreased activities of COMT and MAO. The decreased activity of these enzymes is caused by decreased biosynthesis and by flowage into the blood from the damaged hepatocyte.

Correlation Between Superoxide Radical Production and Hepatic Damage Induced by Bile Duct Ligation

Mun, Kyo-Cheol 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.4

The correlation between superoxide radical production and hepatic damage under cholestasis induced by common bile duct (CBO) ligation was studied in rats. The amount of superoxide radical production in the cholestatic liver was Significantly increased after CBO ligation compared to a sham operation control group. Serum and cytosolic xanthine oxidase (XO) activities were Significantly increased after operation. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities, and the bilirubin level of the cholestatic liver in the subject rats increased after CBO ligation. Severe morphological changes were also noted after CBO ligation. These parameters, which are known to reflect hepatic function in the serum, and the XO activity were significantly correlated with the amount of superoxide radical production. According to these results an increased XO activity includes an increased conversion from xanthine dehydrogenase in the liver, resulting in an increase in oxygen free radical production. These oxygen radicals may play a role in the aggrevation of inflammation and necrosis in the liver under cholestasis induced by CBO ligation.

Correlation Between Superoxide Radical Production and hepatic damage Induced by Bile Duct Ligation

Kyo Cheol Mun 생화학분자생물학회 1994 BMB Reports Vol.27 No.4

The con-elation between superoxide radical production and hepatic damage under cholestasis induced by common bile duct (CBD) ligation was studied in rats. The amount of superoxide radical production in the cholestatic liver was significantly increased after CBD ligation compared to a sham operation control group. Serum and cytosolic xanthine oxidase (XO) activities were significantly increased after operation. Serum alanine aminotransferase (ALT), aspartate aminotransferase (ASS, and alkaline phosphatase (ALP) activities, and the bilirubin level of the cholestatic liver in the subject rats increased after CBD ligation. Severe morphological changes were also noted after CBD legation. These parameters, which are known to reflect hepatic function in the serum, and the XO activity were significantly correlated with the amount of superoxide radical production. According to these results an increased XO activity includes an increased conversion from xanthine dehydrogenase in the liver, resulting in an increase in oxygen free radical production. These oxygen radicals may play a role in the aggravation of inflammation and necrosis in the liver under cholestasis induced by CBD legation.