Heterosigma akashiwo(Raphidophyceae)을 이용한 북만의 조류성장잠재력 시험

김무찬,Kim Mu-Chan 해양환경안전학회 2006 海洋環境安全學會誌 Vol.12 No.2

본 연구는 남해안 북만 해역을 대상으로 조류성장잠재력(AGP) 시험을 통하여 H. akashiwo의 성장제한요인을 평가하였다. 영양물질의 첨가 및 미생물과의 동시배양에 의하면 적조발생 단계별 H. akashiwo의 성장은 서로 다른 제한요인에 의하여 영향을 받는 것으로 나타났다. 적조발생 전의 H. akashiwo 성장은 질산질소 $50{\mu}M$과 연산인 $5{\mu}M$을 복합첨가하면 크게 증가하였지만 인산인을 단독 첨가하거나 미생물과 동시 배양하여도 전혀 영향이 없었다. 적조발생 전의 H. akashiwo 성장제한요인은 질산질소로 나타났다. 적조발생시기의 H. akashiwo 성장은 질산질소 $50{\mu}M$ 또는 인산인 $5{\mu}M$을 단독첨가하면 증가하였지만 미량영양물질이나 $vitamin\;B_{12}$을 첨가해도 전혀 영향이 없었다. 적조발생 시기의 H. akashiwo 성장제한요인은 질산질소와 인산인이 동시에 작용하는 것으로 나타났다. 반면에 적조소멸시기의 H. akashiwo 성장은 질산질소와 인산인을 첨가하면 약간 증가하였지만 미생물과 동시 배양하면 현저히 감소하였다. 그러므로 적조 소멸시기의 H. akashiwo 성장제한은 미생물요인에 의한 것으로 평가되었다. Algal growth potential(AGP) assay using Heterosigma akashiwo was conducted in Pukman Bay. The effects of nutrients and microorganisms on the growth of H. akashiwo were specifically evaluated by the algal bioassay method. The different types of growth response of H. akashiwo to the addition of nutrients, and the co-incubation with microorganisms were clearly observed. Before H. akashiwo red tide occurrence, the growth of H. akashiwo was significantly stimulated by addition of nitrate of $50{\mu}M$ with phosphate of $5{\mu}M$. The addition of single phosphate had no clear effect on the growth of H. akashiwo. And the co-incubation with microorganisms had no clear effect on the growth of H. akashiwo. This result indicates that nitrate potentially limited the growth of H. akashiwo before red tide occurrence. However, during a bloom of H. akashiwo, the growth was significantly stimulated by addition of either nitrate of $50{\mu}M$ or phosphate of $5{\mu}M$. The addition of trace metals and vitamin $B_{12}$ had no clear effect on the growth of H. akashiwo in the period. This result indicates that both nitrate and phosphate potentially limited the growth of H. akashiwo during the bloom. On the other hand, during the termination period of H. akashiwo bloom, the growth of H. akashiwo was slightly stimulated by addition of phosphate and nitrate. But the growth of H. akashiwo was significantly enervated by the co-incubation with microorganisms. This result indicates that microorganisms potentially limited the growth of H. akashiwo in the period of bloom termination.

Risk Assessment of 5-Chloro-2-Methylisothiazol-3(2H)-One/2-Methylisothiazol-3(2H)-One (CMIT/MIT) Used as a Preservative in Cosmetics

Kim, Min Kook,Kim, Kyu-Bong,Lee, Joo Young,Kwack, Seung Jun,Kwon, Yong Chan,Kang, Ji Soo,Kim, Hyung Sik,Lee, Byung-Mu Korean Society of ToxicologyKorea Environmental Mu 2019 Toxicological Research Vol.35 No.2

The mixture of 5-chloro-2-methylisothiazol-3(2H)-one (CMIT) and 2-methylisothiazol-3(2H)-one (MIT), CMIT/MIT, is a preservative in cosmetics. CMIT/MIT is a highly effective preservative; however, it is also a commonly known skin sensitizer. Therefore, in the present study, a risk assessment for safety management of CMIT/MIT was conducted on products containing 0.0015% of CMIT/MIT, which is the maximum MIT level allowed in current products. The no observed adverse effect level (NOAEL) for CMIT/MIT was 2.8 mg/kg bw/day obtained from a two-generation reproductive toxicity test, and the skin sensitization toxicity standard value for CMIT/MIT, or the no expected sensitization induction level (NESIL), was $1.25{\mu}g/cm^2/day$ in humans. According to a calculation of body exposure to cosmetics use, the systemic exposure dosage (SED) was calculated as 0.00423 mg/kg bw/day when leave-on and rinse-off products were considered. Additionally, the consumer exposure level (CEL) amounted to $0.77512{\mu}g/cm^2/day$ for all representative cosmetics and $0.00584{\mu}g/cm^2/day$ for rinse-off products only. As a result, the non-cancer margin of safety (MOS) was calculated as 633, and CMIT/MIT was determined to be safe when all representative cosmetics were evaluated. In addition, the skin sensitization acceptable exposure level (AEL)/CEL was calculated as 0.00538 for all representative cosmetics and 2.14225 for rinse-off products; thus, CMIT/MIT was considered a skin sensitizer when all representative cosmetics were evaluated. Current regulations indicate that CMIT/MIT can only be used at concentrations 0.0015% or less and is prohibited from use in other cosmetics products. According to the results of this risk assessment, the CMIT/MIT regulatory values currently used in cosmetics are evaluated as appropriate.

확장된 GSPN 모형과 그 응용

장태무,전서현,유찬형 東國大學校 1989 論文集 Vol.28 No.-

In the present paper, a new concept called conditional are is added to tranditional GSPN(Generalized Stochastic Petri net) of which effectiveness is proved through an application to an instruction pipeline of general-purpose CPU. The problem in applying Petri nets including GSPN to performance evaluation of computer systems is that the number of nodes of reachability graph increases exponentially with the number of places in the model. Conditional ares proposed in this paper are shown to be a good tool not only for reducing the number of places in the model, but making the task of model construction easy.

농업문제와 농정방향에 관한 소고

리무룡,이찬근 진주여자전문대학 1989 論文集 Vol.11 No.-

저전압/고용량 DRAM 회로를 위한 Half-VDD 전압 발생기 설계

이재형,우엄찬,박무훈,최중경,하판봉,김영희 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-

A half-VDD voltage generator(VHDD) using PMOS pull-up and NMOS pull-down transistors, and each of the gate voltage is able to swing at CMOS voltage level was newly proposed. Designed with these, pull-up current was made to be proportional to (VDD-|V_(TP)|)^2 and pull-down current was made to be proportional to (VDD-|V_(TN)|)^2. Also, being turned on pull-up and pull-down transistors at the same time was prevented by using short-circuit current protection circuit. Test chips which were designed by using 0.35μm CMOS twin-well process technology were fabricated and the result of measurement was suitable for low-boltage DRAMs.

Al-Si/$\{SiC}_{p}$ 복합재료 용탕에서 SiC 입자의 침강

김종찬,권혁무,Kim, Jong-Chan,Gwon, Hyeok-Mu 한국재료학회 1997 한국재료학회지 Vol.7 No.2

Remelting of $A-Si/SiC_{p}$ composites followed by isothermal holding and solidification, leads ro the settling of Sic particles to the bottom of the mold. With the isothermal holding time for molten $A-Si/SiC_{p}$ composites. the particle free zone increases rapidly up to approximately first 30 minutes of the holding time. Experimental resulls of the particle settling confirm that the larger SIC particles sink faster tlun the sniiller particles. An increase in volume fraction of Sic particles decreases the setrling velocity of the particles. AI-xSi/ySiC( x:6~18wt%, y: 3~9wt%, SiC 입자크기: $10~28{\mu}m$) 복합재료를 재용해한 후 항온 유지하고 응고 시킬때 SiC 입자가 몰드의 하부로 침강하는 현상을 계통적으로 조사하였다. AI-Si/SiC 복합재료 용탕을 항온으로 유지하면 입자가 없는 지역은 유지시간이 약 처음 30분 동안 빠르게 증가한다. SiC 입자가 크기가 클수록 SiC입자의 크기가 클수록 SiC입자의 침강속도가 빠르다. 또한 복합재료중 철가한 SiC 입자의 부피분율이 증가하면 입자의 침강속도는 감소한다.

地域經濟活性化를 위한 農村市場開發에 관한 硏究 : 忠南 禮山地域市場을 中心으로

尹畯相,李武鉉,申尹撤,洪性贊,朴奉圭 公州大學校 産業開發硏究所 1994 産業開發硏究 Vol.2 No.-

Since the first year economic development plan, imblanced development of national territory during the economic development process has aggravated remarkable disparity between urban and rural areas. For this reason, this study introduced the development of rural market as one of the development strategies of regional economic to correct the imblance and relieve regional disparity. The purpose of this study were to look into the present situation of traditional rural market and to analyze the problems for the rural development in view of the current road situations in the rural areas and status of marketing facilities in market center and to measure the degree of rural area people's satisfaction to the rural market and to suggest the development plan of activating for rural market center. To collect data, the Yesan Gun rural market were selected and a total of 591 residents and 178 merchants were sampled as final subjects of survey. Interview and/or questionnaire method was the main method of data collection. The major findings of this study were as follows: 1) The population of Yesan Gun has decreased 2.85 persons per year from in 1984, but the number of vehicles were increased 2.85 times than in 1989. And the parking capacity of Yesan Eup was only 129 vehicles which were below of the total vehicles of Yesan Gun area (14,352). The number of Yesan area markets were 8 markets which consisted of 1 permanent market and 7 periodic markets. But as the economy has been developed, the conditions of rural markets have been changing. That is, the role of periodic market tends to be weakened with the growth of large cities, reinforcement of permanent markets, development of traffic facilities, large production, preferring high quality goods. 3) Generally, the people of Yesan Gun were attached for purchasing the necessaries of life and comodities and farming materials to ① Yesan Eup ② Seoul ③ Chonan Shi ④ Hongsung gun ⑤ Daejeon Shi. 4) The degree of community people's satisfaction to the town market presented 26.6% which meaned higher than dissatisfaction(9.5%). And the degree of people's inconvenience of accessibility to market center by transferring of bus terminal presented 64.2% which meaned higher than average. 5) The degree of people's dissatisfaction of traffic system in Yesan gun area presented followings: the road networks(61.5%), parking facility(68%), bus route(54%). 6) By the survey of merchants of Yesan gun area, on the average, it showed followings: the size of store was 6-10 pyeong(40%), the type of enterprise was retail dealer(70.9%), and purchasing place was wholesale dealer from Seoul(51.8%). 7) And they thought that Yesan town market were too small(70.8%), and the diversifying of goods was not enough(35.5%). 8) The merchants of Yesan area showed that the degree of into venience to traffic system was 72.7% and its to parking facility was 83.3%. And they complained that community administration agency have not tried to activate market(79.1%). Based on the results of this study, the following recommendation were made for activating regional economy and community market. That is, it is to strengthen the economic function in community market so as to suffice the economic and social activities for settlement area as their living space. And it is necessary to improve the interrelationship of the central functions. 1) So, the accessibility between market center and its surrounding areas must be rised. ① the enforcing preferentially to pave the bus terminal and market center to link two place. ② the running of shuttle-bus two place every regular market day. ③ the widening and connecting the road to rise accessibility between market center and its surrounding area. ④ the enforcement of parking facility around market center. ⑤ the constructing marketing centered area as intergrated body of various marketing facilities in the adjacent site is the centered area planned to achieve efficient marketing. 2) The modernization and specialization market center facilities. 3) The strengthening of drawing surrounding area people into Yesan market center. So, the following recommended: ① the development of special products market ② inducing special projects ③ the development tour and leisure course drawing industrial facilities. 4) And it needs to enforce community people's local patriotism and to support community markets by administration agency.

C-단백 결핍에 의한 응고항진으로 발생한 폐동맥 혈전색전증

조준휘,박찬우,조병렬,최대희,조성준,이성호,황성오,안희철,안무업,서정열,유기철 대한응급의학회 2003 대한응급의학회지 Vol.14 No.1

A pulmonary thromboembolism usually results from a serious complication of deep venous thrombosis (DVT). However, several prothrombotic genetic risk factors are known to predispose a patient to thrombotic events, with manifestation at a young age. Protein C and S deficiencies are known to increase the risk of venous thrombosis and pulmonary thromboembolism. We report a case of a young patient with protein C and S deficiencies suffering from a massive pulmonary thromboembolism.

적조생물 살조세균 탐색 -IV. 살조세균 Micrococcus sp. LG-5가 생산하는 살조물질의 특성과 해양생물에 미치는 영향-

정성윤,박영태,김무찬,최석철,성희경,김재영,김태운,이원재,JEONG Seong-Youn,PARK Young-Tae,KIM Mu-Chan,CHOI Seok-Cheol,SEONG Hee-Kyung,KIM Jai-Young,KIM Tae-Un,LEE Won-Jae 한국수산과학회 2000 한국수산과학회지 Vol.33 No.4

적조생물을 살조시키는 해양세균 Microcorcus sp. LG-5가 생산하는 세포외 분비 살조물질의 최적 생성조건, 살조물질의 특성과 한외여과 분획별 살조활성 및 해양생물에 미치는 영향을 조사함으로서 자연생태 조화형, 환경 친화적 적조방제 기술개발의 기초 자료를 제공하고자 연구한 결과를 요약하면 다음과 같다. Micrococcus sp. LG-5의 살조물질 생성 최적온도는 $20{\~}30^{\circ}C$로 살조활성이 $100{\%}$였으며, 살조물질 생성 최적 pH는 7.0, 최저 염농도는 $3.0{\%}$였다. 또한 세포외 분비 살조물질의 $IC_(50)$ 값은 $0.482{\%}$였다. 세포외 분비 살조물질의 열 안정성을 실험한 결과, 열처리한 배양여과액 $1{\%}$ 첨가시 12시간 후에 $31.4{\%}$, 24시간 후 $79.7{\%}$, 36시간 후 $94.6{\%}$의 살조활성을 보였고 $5{\%}, 10{\%}$의 경우 각자 36시간, 24시간 후에 $100{\%}$ 살조되었으며, 열처리하지 않은 배양여과액을 넣었을 때와 비교해볼 때 살조력에 유의한 차이는 없었다. 이와 같이 Micrococcus sp. LG-5가 생산, 분비하는 살조물질은 열에 안정한 내열성 물질로 판단된다. 또한 대사산물의 pH 안정성은 $pH 6.0{\~}8.0$에서 거의 $100{\%}$의 살조활성을 보였으며 $pH 5.0{\~}10.0$에서 살조활성이 $85{\%}$ 이상으로 나타나 살조물질은 약산성과 알카리에서 살조활성이 안정하였다. 또한 한외여과의 결과, 분자량 $>10,000, 10,000{\~}1,000, <1,000$의 3개의 분획 모두 살조활성이 있었으며, 각 분획 모두 열에 대단히 안정하였다. 각 분획별 $IC_(50)$ 값은 분자량 1,000 이상에서 강한 살조활성이 나타났으며 분자량 1,000 이하에서는 상대적으로 낮은 살조활성을 보였다. Micrococcus sp. LG-5가 해양세균에 미치는 영향은 E. faecalis, E. coli, K. pneumoniae 및 V. alginolyticus에 대해서는 항균력을 보였으나, P. aeruginosa, P. fluorescens, S. typhi, S. aureus, V. cholerae 및 V parahaemolyticus에 대해서는 전혀 항균력을 보이지 않았다. 그리고 적조생물에 미치는 영향은 A. tamarense, E. gymnastica, G. catenatum, G. niikimotoi, G. sanguineum, G. impudicum, H. triquetra, H akashiwo, P. micans와 Pyramimonas sp.에 대해서는 살조활성을 보였으나 Chlamydomonas sp., C. closterium, P. mininum, P. triestimum, Pseudonitzschia sp.와 S. trochoidea에 대해서는 살조활성을 보이지 않았다. 즉, 적조생물에 따라 살조활성이 다양하게 나타나 종 특이성이 낮았다. 먹이생물에 미치는 영향은 1 galbana를 제외하고 실험한 모든 먹이생물에 별다른 영향이 없거나 오히려 성장을 촉진시켰고, 또한 살조세균 배양여과액을 넙치 사육수에 $10{\%}$까지 투여하였을 때 양식 넙치에 미치는 유해한 생리학적 영향은 없었다. 이상의 연구 결과 살조세균 Micrococcus sp. LG-5의 배양여과액은 조사된 적조생물을 제외한 해양세균, 치${\cdot}$자어의 먹이생물 및 양식 넙치에 유해한 영향은 미치지 않을 것으로 판단된다. An algicidal bacterium, Micrococcus sp. LG-5 against the harmful dinoflagellate, Cochlodinium polykrikoides was isolated. The optimal conditions for the highest algicidal activity of bacterial culture filtrate showed in the range of $20{\~}30^{\circ}C$, at pH 7.0 and $3.0{\%}$ of NaCl concentration. In addition, $IC_(50)(mean of 50{\%} inhibitory concentration)$ of the culture filtrate against C. polykrikoides after incubation of 5 days was $0.482{\%}$. To investigate heat and pH stability of the culture filtrate of Micrococcus sp. LG-5, the culture filtrate ($pore size, 0.1 {\mu}m$) was heated to $121^{\circ}C for 15 min$ and adjusted pH from 2.0 to 10.0. There were no significant changes in algicidal activity by heat treatment and the pH change between pH from 5.0 to 10.0. The algicidal substances produced from Micrococcus sp. LG-5 were mainly detected in the fraction of $10,000{\~}1,000$ MWCO (molecular weight cut-off). The culture filtrate of Micrococous sp. LG-5 showed antimicrobial activity against Enterococcus faecalis, Escheiichia coli, Uebsiella pneunioniae and Vibrio altinolyticus, but did not show against Pseudomonas aeminosa, P. Buorescens, Salmonella typhi, Staphylococcus aureus, V. cholerae and V parahaemolyicus. The culture filtrate of Micrococcus sp. LG-5 was examined against 16 phytoplankton species and showed the algicidal activity against Ajexandzium tuarense, Eutreptiella Drnnastin, Gymnodinium catenatum, G. mikimotoi, G. sanguineum, eyodinium impuaicum, Heterocapsa triquetra, Heterosipa akashiwo, Prorocentrum micans and Pyraminonas sp.. However no algicidal effects of Micrococcus sp. LG-5 were observed against Chlamydomonas sp., Cylindrotheoa closterium, P. mininum, P. triestimum, Pseudonieschia sp. and Sczipuiella trochoidea. On the other hand, algicidal activity on the tested marinelivefood was not detected except for Isochrysis galbana. In addition, physiological responses of cultured olive flounder (Paralichthys oliraceus) exposed to $1 and 10{\%}$ of the culture filtrate of Micrococcus sp. LG-5 were measured. There were no clear changes in AST, GGT, creatinine, urea, total cholesterol, total protein, albumine, $Mg^(+2), Ca^(+2), Na^+, K^+, and Cl^-$. These results indicate that olive flounders were not affected when they were exposed to the culture filtrate of Micrococcus sp. LG-5.

Original Articles : Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

( Mu Chan Kim ),( Chan Il Park ) 한국수산학회 2005 Fisheries and Aquatic Sciences Vol.8 No.2