저혈청 배지에서 인간 전골수세포(HL-60)를 이용한 tPA 생산과 세포사멸기작에 관한 연구

김현구,성기돈,김태호,안주희,함문선,박진서,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

tPA 생산과 세포 사멸의 연구를 하기 위해서 인간 전골수세포가 배양되었다. 연속 관류 배양하에서 최대 tPA 생산량은 324 ng/ml이며 최대 세포 농도는 5.27×10 exp (6) cells/ml였다. Phorbol ester를 넣었을 때 tPA 생산량은 420 ng/ml까지 향상되었다. 형광 현미경으로 관찰했을 때, apoptosis는 세포 배양 초기부터 중반까지 우세했고 빠른 교반 속도에서도 우세했다. 그러나 necrosis는 세포배양 말기와 동결된 세포가 해동 되었을 때 우세했다. HL-60 was cultivated to produce tPA (tissue-type plasminogen activator) and study the mechanism of cell death. Maximum cell density and tPA production were obtained as 5.27×10 exp (6) cells/ml and 324 ng/ml, respectively under perfusion cultivation. tPA production was enhanced to 420 ng/ml in adding 160 nM of phorbol ester. The cells were gradually differentiated to granulocytes rather than proliferation. By Fluorescent microscope, apoptosis was prevailed except the death phase and in high agitation speed, but necrosis was prevailed in thawed cells and during the latter periods of the cultivation. It was also proved that tPA was most produced in apoptosis. To obtain higher tPA productivity, the cells must be maintained in apoptosis, not necrosis phase when the cells were dying.

북방형목초의 예취시 초장에 따른 저장 탄수화물 함량과 생산성에 관한 연구

김태구,김문철 제주대학교 농과대학 제주도축산문제연구소 1986 畜産論叢 Vol.1 No.1

This study was carried out to investigate the effects of different plant helght on the total water soluble carbohydrate in the leaf, stem, and root of orchardgrass (Dactylis glomerota), perennial ryegrass (Lolium perenne) and red top (Agrosfis alba), grasses of temperate origin grown in experimental plots at Cheju National University, from September, 1984 to September, 1985. The results obtaind were as follows; 1. The dry matter produrtlvity of orchardgrass was 1,449kg/10a, of perennial ryegrass, 757kg/10a and of red top, 531kg 10a. The dry matter yield of orchardgrass was higher than the other grasses and when compared with the others was statistically highly significant(P<0.01) 2. The total dry matter yield varled according to plant height cutting. There were highly significant differences between grasses 20cm and 30cm or 40cm tall. However, there were no statistically significant differences between grasses 30cm and 40cm in height. 3. During the hot season, when grasses were cut for the third time. the total water soluble carbohydrate decreased in orchardgrass and perennial ryegrass but increased in red top. 4. Except for a slightly Increased total water soluble Carbohydrate in all parts of grasses at 2Ocm tall, there were no differences in carbohydrate content at other helghts. 5. The dry matter yield of orchardgrass and perennlal ryegrass at 2Ocm rose as the total water soluble carbohydrate increased but decreased in red top at all plant height. Statlstically, there was no significant correlation between total water soluble carbohydrate and dry matter yield of grasses.

비외상성 두개내 출혈 환자에서 심근손상의 발생과 예후에 미치는 영향

강구현,황성호,이강현,조준휘,김성환,문중범,박해상,이서영,이성수,김헌주 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Objective: The aim of this study was to investigate the clinical significance of myocardial injuries in patients with nontraumatic intracranial hemorrhage by identifying the occurrence of myocardial injury and defining its correlation with subsequent cardiovascular events. Subjects and methods: One hundred twenty-four patients with nontraumatic intracraninal hemorrhage presented to the emergency department within six hours from onset of symptoms were enrolled. Brain CT, serial electrocardiography, and echocardiography were done at the emergency center. Blood samples for troponin I and creatine kinase(CK)-MB were drawn immediately and eight hours after admission, Troponin I and CK-MB were measured using a chemiluminescent immunoassay, respectively. Results: Electrocardiographic and echocardiography abnormalities were found in 65 cases(52.4%) and 21 cases(17%), respectively. Serum troponin I and creative kinase-MB were increased in 35 cases (28.2%) and in 58 cases(46.8%), respectively. Abnormal findings of echocardiography and ECG, as well as elevated levels of serum troponin I and creative kinase-MB, were associated with an increased risk of cardiovascular event and survival. Logistic regression analysis revealed that an abnormal echcocardiographic finding and elevation of serum troponin I were factors associated with the occurrence an adverse cardiovascular event and that electrocardiographic abnormalities and initial mental status were factors associated with poor prognosis. Conclusion: This study reveals that actual myocardial injury develops in a significant proportion of patients with nontraumatic intracranial hemorrhage and that the development of the myocardial injury is associated with an adverse cardiovascular event that occurs during admission.

하중속도가 암석의 강도 및 변형특성에 미치는 영향에 관한 연구

강문구,김재동,임한욱 江原大學校 産業技術硏究所 1990 産業技術硏究 Vol.10 No.-

Uniaxial compression tests are performed under various loading rates to study the influence of loading rate on the compressive strengths and deformation behaviours. The rock samples adopted in this experiments are Onyang granite, Hambaek sandstone, Hambaek shale and Donghae limestone. Total 120 specimens are prepared for this study. As the loading rate increases from static to dynamic state which is about the level of 800~1,400 kgf/㎠/sec, the uniaxial compressive strengths are also increased within the range of 40%. And Young's modulus and Poisson's ratio show similar trends but have a little lower rates of increase when compared with that of uniaxial compressive strength.

폴리에스테르 섬유 고속방사중 필라멘트의 냉각과정 분석

윤문구,박준원,이승윤 건국대학교 산업기술연구원 1998 건국기술연구논문지 Vol.23 No.-

High speed fiber spinning of polyethylene terephthalate(PET) was simulated at take-up speed of 6000m/min utilizing generalized steady state governing equations of melt spinning and some other assumed relationship for structure formation. In the present paper, the effect of changes of quench air temperature on the spinning line were mainly discussed. Distance of necking point and stress from the spinneret was elongated when higher quench air temperature was applied. Changes of necking point and stress may affect the development of PET fiber structure in the high speed threadline.

제주도내 마을공동목장의 초지관리 이용 및 식생상태의 조사

김문철,박희석,이수일,김태구 제주대학교 농과대학 제주도축산문제연구소 1986 畜産論叢 Vol.1 No.1

Pasture management and utilization for beef production, and botanical composition of the pasture on 81 co-operate village farmsin Cheju-Do were surveyed through questionaires and site visits. The study period was 28 days from October 2 to October 30, 1985. The pasture consisted of 45% improved grassland, in which 24.3% of grass species were imported. The improved pastures were fertilized once a year 100-150kg urea/ha. Grazing period of the pastures was about 5 months, beginning later April or early May(60.5% of the pastures) and ending early-or mid-November (80.3%). Distribution of types of vegetation was, in order of magnitude, native pasture (38.7%), improved pasture (24.3%), shrub (10.0%), unfavorable weeds(8.3%), Speies found with the highest percentage was Zoysia japonica(73.9%) in the native pasture, Festuca arundinacea(40.3%) in the improved pasture and Pteridium aquilium (54.4%) among unfavorable weeds.

골화석증 1례 보고

오정환,이문구 건국대학교 의과학연구소 1994 건국의과학학술지 Vol.4 No.-

Osteopetrosis, also known as Albers-Schonberg disease, marble bone, osteosclerosis fragilis, or chalk bone, is a rare hereditary, congenital and familial disease, characterized by a marked increase in the radiodensity of the bone and by an abnormality in the shape of the long bone, especially the metaphysis. It may occur in autosomal recessive form with severe, generalized bone changes in the new born infant that result in early death or in a more benign autosomal dominant form with less extensive bone involvement and relatively good prognosis. We experienced a case of a benign autosomal dominant form of osteopetrosis incidentally and reporting this case with a review of the literatures.

광합성 세균을 이용한 주정 폐수처리

김기은,조문구 又石大學校 1995 論文集 Vol.17 No.-

Five photosynthetic bacteria were finally isolated from nature. They showed higher degrading abilily of major organic acids in distillery wastewater such as acetic acid, propionicacid, and butyric acid under anaerobic light corditions(5,000lux, 30℃). Acetic and propionic acids were degraded in first, and then butylic acid was followed with the increasement of pH up to 8.5, All of the organic acids were completely degraded within 60 hours, and the highest value of the specific growth rate(μmax) was 0.29h^-1.

주개 이온을 첨가한 강유전체 Bi₄Ti₃O_12(BIT) 박막의 특성

최은경,박문흠,김두회,구영건,김상수 昌原大學校 基礎科學硏究所 2001 基礎科學硏究所論文集 Vol.13 No.-

Bismuth layered structure ferroelectric thin films, BIT and donor (V, Nb and W) doped BIT, were prepared on the Pt/Ti/SiO2/Si substrates by sol-gel method. BIT film was characterized by strong c-axis prepared orientation. In the case of donor doped BIT thin films, the degree of c-axis prepared orientation decreased systematically V-BIT→W-BIT→Nb-BIT and Nb-BIT film was shown random orientation. Surface morphologies of BIT and V-BIT films had a plate-like and Nb-BIT and W-BIT had a mixture of plate-like and rod-like. In the results of ferroelectric hysteresis loops, the remanent polarization (2Pr) of random-oriented Nb-BIT capacitor was 26 μC/cm2, and this value was over two times higher than that of 12 μC/cm2 for BIT capacitor. The polarization fatigue up to 4.5×1010 read/write switching cycles of W-BIT capacitor was shown superior to the other capacitors. The polarization of the W-BIT capacitor decreased only 10% of the initial value after 4.5×1010 switching cycles.

팔영산 삼림식생과 토양환경

이호준,김종홍,배병호,박문수,전영문,강재구,신정식 建國大學校基礎科學硏究所 1997 理學論集 Vol.22 No.-

전라남도 고흥군 소재 팔영산 (608.6 m) 일대의 삼림군락에 대한 색생조사를 1993년 10월부터 1995년 7월까지 실시하였다. 조사한 자료는 Z-M 방식에 의해 종조성표를 작성하였다. 이에 따라 삼림군락은 신갈나무군락, 졸참나무군락, 굴참나무군락, 서어나무군락, 소나무군락등 5 개 군락으로 분류되었다. 입지와 환경과의 유기적 관계를 조사하기 위해 토양을 분석한 결과, 신갈나무군락은 토양의 비옥도가 높은 산의 능선 부위에 집중 분포하였으며, 굴참나무군락과 소나무군락은 토양의 비옥도가 낮고 비교적 건조한 고도 400 m 이하 지역에 주로 군락을 형성하고 있었다. 또한 졸참나무군락은 고도 300 - 550 m 이내에서 부분적으로 나타났으며, 서어나무군락은 토양의 비옥도가 양호한 고도 300 - 550 m 사이의 골짜기 주변에 국지적으로 분포하였다. 팔영산 삼림식생의 특징은 상록활엽수림이 형성될 수 있는 지역이나 후박나무, 동백나무, 사스레피나무 등의 몇몇 종을 제외하고는 상록활엽수의 분포를 확인할 수 없었으며, 일부 식재림을 제외한 대부분의 지역이 낙엽활엽수와 소나무의 혼효림으로 구성되어 있었다. The phytosociological study forest vegetation on Mt. Palyoung (608.6 m) was investigated from October 1993 to July 1995 in Koheug-Gun, Cheonnam province. According to Z-M method, the floristic composition table of the forest vegetation was divided into five community as follows; Quercus mongolica community, Quercus serrata community, Quercus variabilis community, Pinus densiflora community, Carpinus laxiflora community. The soil was analyzed to investigated the soil conditions and soil fertility. As a result, the organic matters which showing soil fertility were found to be low Quercus variabilis and Pinus densiflora community at comparatively xerophic conditions, below 400 m altitude, while they were increased in Quercus mongolica community on mountain ridge, and that Quercus serrata community was appeared 300-550 m altitude around mountain stream to moderate soilfertility. The forest vegetation of Mt. Palyoung area unconfirmed capable of evergreen broad-leaved forest, but appeared to Machilus thunbergii, Camellia japonica and Eurya japonica species etc. On the other hand, surveyed site's vegetation was distributed mixed forest of deciduous leaved forest and Pinus desiflora, except for some afforestation.