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等電點 電氣泳動法에 의한 벼 種子內 esterase 同位酵素 遺傳子 分析
Moo Young Eun(殷茂永),Yong Kwon Kim(金容權),Yong Gu Cho(趙龍九),Tae Young Chung(鄭泰英) 한국육종학회 1990 한국육종학회지 Vol.22 No.3
The inheritance of esterase isozvmes in rice was identified by controlled crosses and segregation analysis with esterase phenotypes classified. Using the method of isoelectric focusing(IEF), four esterase loci and their allozymes were identified and named as Est Ⅰ-1, Est Ⅰ-2, Est Ⅰ-3, and Est Ⅰ-4. There allozymes at each locus were found. Est Ⅰ-1 locus was controlled by two active dimeric alleles and a null allele. Two monomeric alleles and a null allele were found at both Est Ⅰ-2 and Est Ⅰ-3 locus. Two dimeric alleles and one weak allele were recognized at Est Ⅰ-4 locus. Linkage tests showed that four esterase loci were independent each other.
Moo Young Eun(殷茂永),Yong Kwon Kim(金容權),Yong Gu Cho(趙龍九),Young Woo Kim(金永雨),Tae Young Chung(鄭泰英),Hae Chune Choi(崔海椿) 한국육종학회 1989 한국육종학회지 Vol.21 No.4
Using the method of isoelectric focusing in polyacrylamide horizontal slab gels, Korean native rice cultivars, which were collected from Gene Bank of the Rural Development Administration, were analyzed for their five isozyme patterns and classified into the enzymatic groups. Among 259 Korean native rice cultivars screened, 5 esterase(EST), 4 phosphoglucose isomerase(PGI), 4 malic enzyme(ME), 2 hexokinase(HK) and 5 β-amylase(β-AMY) isozyme phenotypes were distinguished. Total 15 enzymatic groups could be classified by combining five enzyme phenotypes. The eleventh enzymatic group 〔EST-ⅡC:ME-Ⅲ:PGI-Ⅱ:HK-Ⅱ:β-AMY-Ⅰ〕 was most frequent (111 cultivars), but only one to three cultivars were found in the 1st-6th, 9th and 13th groups. Over 96% of Korean native rice cultivars were showed the isozyme phenotypes of 〔EST-ⅡC:ME-Ⅲ:PGI-Ⅱ〕.
벼 밀양 23호 $\times$ 기호벼 재조합 자식계통의 지역에 따른 품질 특성 관련 QTL 분석
곽태순,여준환,은무영,차영순,Kwak Tae-Soon,Yeo Jun-Hwan,Eun Moo-Young,Cha Young-Soon 한국작물학회 2004 한국작물학회지 Vol.49 No.6
M/G RIL 164계통과 그 유전자지도를 이용하여 지역에 따른 벼의 품질과 관련된 양적형질 유전자좌(QTL)를 분석한 결과를 보면 다음과 같다. M/G RIL 164계통의 지역에 따른 단백질함량, 아밀로오스 함량, 지방산함량 및 식미평가치에 있어 빈도분포는 정규분포에 가까운 연속변이를 보였으며, 양친의 범위를 벗어나는 초월분리 현상을 나타내었다. 또한 지역에 따라 품질형질의 분포범위의 폭이 다양하게 나타났으며, 단백질함량은 원주>익산>대구의 순으로, 아밀로오스함량, 지방산함량 및 식미평가치는 대구>익산>원주의 순으로 나타났다. 품질에 관련된 QTLs분석에 있어 단백질함량과 관련하여서는 8개의 QTLs를 확인하였으며, 1번 염색체에서 2개, 3번, 6번, 7번 염색체에서 각각 1개, 8번 염색체에서 3개의 QTLs를 확인할 수 있었으며, 이들 8개의 QTLs가 설명할 수 있는 표현형 변이는 $6.0\~15.2\%$로 나타났다. 아밀로오스함량과 관련하여 6번 염색체에서 1개, 7번 염색체에서 2개의 QTLs를 확인하였다. 3개의 QTLs가 설명할 수 있는 표현형 변이는 $7.3\~24.4\%$로 나타났다. 지방산함량과 관련하여서는 2번과 6번 염색체에서 각각 깨, 3번과 7번 염색체에서 각각 1개의 QTLs를 분석하였으며, 6개의 QTLs로 설명할 수 있는 표현형 변이는 $5.5\~14.0\%$를 보였다. 식미평가치와 관련된 QTLs는 2번과 6번 염색체에서 각각 1개, 7번과 8번 염색체에서 각각 2개의 QTLs가 분석되었으며, 그 6개의 표현형 변이는 $5.5\~10.3\%$로 나타났다. The purpose of this study was to locate the quantitative trait loci (QTL) associated with quality properties in the recombinant inbred lines derived from the 'Milyang 23' and 'Gihobyeo' cross. Four quality-related traits; protein content, amylose content, fat acid content and sensory value were measured. Eight QTLs for protein content were detected on chromosomes 1 (two loci), 3, 6, 7 and 8 (three loci), each accounting for $6.0\%\~15.2\%$ of the phenotypic variation. Three QTLs for amylose content were detected on chromosomes 6 and 7 (two loci), each explaining from $7.3\%\;to\;24.4\%$ of the phenotypic variation. Six QTLs for fat acid content were detected on chromosomes 2 (two loci), 3, 6 (two loci) and 7, each explaining form $5.5\%\;to\;14.0\%$ of the phenotypic variation. Six QTLs for sensory value were detected on chromosomes 2, 6, 7(two loci) and 8 (two loci), each accounting for $5.5\%\~10.3\%$ of the phenotypic variation.
밀양23호와 기호벼 교잡 재조합자식계통(RILs)의 품질관련 특성 변이
강현중,김영두,김현순,이영태,은무영,Kang, Hyeon-Jung,Kim, Young-Doo,Kim, Hyun-Soon,Lee, Young-Tae,Eun, Moo-Young 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1
통일형인 밀양23호와 자포니카인 기호벼를 교잡한 재조합자식계통을 대상으로 품질관련 특성 변이를 살펴본 결과 공시계통들에 대한 품질 관련 형질들의 변이 분포는 매우 폭 넓고 다양했으며 대부분의 형질에서 연속적인 정규분포를 보였다. 조사된 형질간의 상관관계는 립의 두께에 대하여 립폭, 심백과 복백은 고도의 정의 상관관계를 나타냈으나 심백과 알카러 붕괴도는 고도의 부의 상관관계를 보였다. 단백질 함량과는 아밀로스, Mg/K 비율에서 고도의 부의 상관을 나타냈으며, K와 지방 함량과는 고도의 정의 상관을 보였다. 밥의 물리성에서는 딱딱한 정도를 나타내는 경도와 부착성, 탄력성, 검성, 저작성에서 고도의 정의 상관을 보였다. The rice quality related traits including physico-chemical traits were evaluated with one hundred sixty-four MG RILs derived from the cross between Milyang 23, Indica/Japonica hybrid type, and Gihobyeo, Japonica type. The variation distribution of all traits examined approximately fit normal distribution and transgressive segregants over parents were observed for all traits. The occurrence of such transgression could be associated with the interactions of complementary QTL alleles from two parents. However in this study, it could not be concluded that our results of segregation were due to either complementary gene effects or overdominance of a major gene. These factors should be verified by further studies. Correlations between traits were evaluated by regressing phenotypic values of one trait on those of another traits. There are highly significant correlation between grain thickness with grain width, white core and white belly. But between white belly and alkali digestion value showed highly negative significant correlation. Contents of protein showed highly negative correlation with amylose and Mg/K ratio but showed highly correlation with K and Fat contents. Hardness of cooked rice showed highly correlation with adhesiveness, elasticity, gumminess, chewiness.
토양에서 분리된 Xanthomonas sp. 의 Chitinase 유전자 cloning 과 E.coli 에서의 발현
황철원,김호상,성기영,은무영 ( Cher Won Hwang,Ho Sang Kim,Ki Young Seong,Moo Young Eun ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.2
Xanthomonas sp. isolated from soil exhibited cell wall lytic activity of Candida albicans and secreted chitinase in chitin media. Especially, the chitinase activity was induced by chitin and reached a maximum level at 3 days culture in chitin media. We constructed genomic library of Xanthomonas sp. using cosmid vector in E. coli. Oligonucleotide probe, was synthesized from the consensus sequence corresponding to chitinase active site, which was derived from the comparison of amino acid sequences of bacterial chitinase genes. Using this oligonucleotide probe, we screened the genomic library. By restriction enzyme mapping of the positive clones, we identified 4 independent clones which may contain the chitinase gene. One of the clones, named pXCH1 (1.2 kb insert), was further analyzed. Northern blot analysis indicated that is transcripts, 1 kb and 0.8 kb, were induced by chitin. When the cloned gene was induced by IPTG in E.coli cell, chitinase activity which was secreted onto culture media was not observed. However, when the cell was disrupted by using sonicator and then centrifuged, the supernatant exhibited chitinase activity. SDS-PAGE of the supernatant indicated that about 35 kDa protein was induced by IPTG. From these results, it was concluded that the cloned DNA was one of the chitinase genes of Xanthomonas sp.
이강섭,박성한,윤도원,안병옥,김창국,한창덕,이기환,박동수,은무영,윤웅한,Lee, Gang-Seob,Park, Sung-Han,Yun, Do-Won,Ahn, Byoung-Ohg,Kim, Chang-Kug,Han, Chang-Deok,Yi, Gi-Hwan,Park, Dong-Soo,Eun, Moo-Young,Yoon, Ung-Han 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2
Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.