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Mochammad Amrun Hidayat,Diah Ayu Maharani,Djoko Agus Purwanto,Bambang Kuswandi,Mochammad Yuwono 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2
A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2- benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method.
Purwanto, Mochammad,Widiastuti, Nurul,Gunawan, Adrian Materials Research Society of Korea 2021 한국재료학회지 Vol.31 No.7
Chitosan powder is synthesized by a deasetylation process of chitin, obtained from processing of dried shrimp shell powder. Subsequently, chitosan (CS) membranes filled by montmorillonite (MMT) particles and phosphotungstic acid are prepared, and characterized by FT-IR and SEM. The morphology, obtained by SEM for the composite membrane, showed that MMT filler is successfully incorporated and relatively well dispersed in the chitosan polymer matrix. Water and methanol uptake for the CS/MMT composite membranes decrease with increasing MMT loadings, but IEC value increases. In all prepared CS/MMT composite membranes, the CS membrane filled by 5 wt% MMT particles exhibits the best proton conductivity, while that with 10 wt% MMT loading exhibits the lowest methanol permeability; these values are 2.67 mS·cm<sup>-1</sup> and 3.40 × 10<sup>-7</sup> cm<sup>2</sup>·s<sup>-1</sup>, respectively. The best membrane selectivity is shown in the CS/MMT10 composite membrane; this shows that 10 wt% filled MMT is the optimum loading to improve the performance of the chitosan composite membrane. These characteristics make the developed chitosan composite membranes a promising electrolyte for direct methanol fuel cell (DMFC) application.
Effect of Load Condition on Turning Performance of a VLCC in Adverse Weather Conditions
Zaky, Mochammad,Yasukawa, Hironori Korean Society of Ocean Engineers 2018 Journal of advanced research in ocean engineering Vol.4 No.2
The load condition significantly influences ship maneuverability in calm water. In this research, the effect of the load condition on turning performance of a very large crude oil carrier (VLCC) sailing in adverse weather conditions is investigated by an MMG-based maneuvering simulation method. The relative drift direction of the ship in turning to the wave direction is $20^{\circ}-30^{\circ}$ in ballast load condition (NB) and full load condition (DF) with a rudder angle $35^{\circ}$ and almost constant for any wind (wave) directions. The drifting displacement in turning under NB becomes larger than that under DF at the same environmental condition. Advance $A_d$ and tactical diameter $D_t$ become significantly small with an increasing Beaufort scale in head wind and waves when approaching, although $A_d$ and $D_t$ are almost constant in following wind and waves. In beam wind and waves, the tendency depends on the plus and minus of the rudder angle.