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PIG3 Regulates p53 Stability by Suppressing Its MDM2-Mediated Ubiquitination
( Min Jin ),( Seon-joo Park ),( Seok Won Kim ),( Hye Rim Kim ),( Jin Won Hyun ),( Jung-hee Lee ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4
Under normal, non-stressed conditions, intracellular p53 is continually ubiquitinated by MDM2 and targeted for degradation. However, in response to severe genotoxic stress, p53 protein levels are markedly increased and apoptotic cell death is triggered. Inhibiting the ubiquitination of p53 under conditions where DNA damage has occurred is therefore crucial for preventing the development of cancer, because if cells with severely damaged genomes are not removed from the population, uncontrolled growth can result. However, questions remain about the cellular mechanisms underlying the regulation of p53 stability. In this study, we show that p53-inducible gene 3 (PIG3), which is a transcriptional target of p53, regulates p53 stability. Overexpression of PIG3 stabilized both endogenous and transfected wild-type p53, whereas a knockdown of PIG3 lead to a reduction in both endogenous and UV-induced p53 levels in p53-proficient human cancer cells. Using both in vivo and in vitro ubiquitination assays, we found that PIG3 suppressed both ubiquitination- and MDM2-dependent proteasomal degradation of p53. Notably, we demonstrate that PIG3 interacts directly with MDM2 and promoted MDM2 ubiquitination. Moreover, elimination of endogenous PIG3 in p53-proficient HCT116 cells decreased p53 phosphorylation in response to UV irradiation. These results suggest an important role for PIG3 in regulating intracellular p53 levels through the inhibition of p53 ubiquitination.
조민진 ( Jo¸ Min-jin ),김재엽 ( Kim¸ Jae-yeob ) 한국건축시공학회 2021 한국건축시공학회 학술발표대회 논문집 Vol.21 No.2
Based on changes in capacity demanded by society as a result of the 4th industrial revolution, as well as changes in the environment due to COVID19, it is necessary to adopt new educational methods in Korean universities. In response to such changes, there have been attempts to apply innovative teaching methods, such as flipped learning and blended learning. However, most studies are limited to either learning effects and/or satisfaction. Accordingly, this study seeks to contribute to the changing educational methods by developing online and offline blended building construction curriculum. The results of this study are as follows: combined education was made possible by developing a curriculum by dividing the online and offline environments. It is determined that the developed curriculum will be efficient and appropriate for the new environments.
Minjin Cho,Su-Jin Kim,Man Soo Joun 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.4
A single-stage, precision, symmetrical L-bending process is developed, which employs a forming-load control die for progressive sheet-metal forming of a circuit-breaker. It is targeted to replace the traditional two-stage symmetrical L-bending process, which features rough bending followed by fi ne sizing/adjustment to meet the required geometrical tolerances. The new process requires optimization since the die controls springback caused by the pressing load imparted to the shoulder by the same die. The Taguchi method is thus used to optimize the process design using an elastoplastic finite element method in terms of the tilt angle after springback and the leg length of the resulting bent region. The finite element predictions of the optimized process are in excellent agreement with the experimental data and there are no cases of failure.
L2 Writers’ Use of Signaling Nouns: A Focus on Modification and L2 Proficiency
Minjin Kim,Sun-Young Oh 한국영어학회 2023 영어학 Vol.23 No.-
This study investigates the relationship between Korean students’ English proficiency and their utilization of signaling nouns (SNs) and SN modifications in argumentative essays. Data were collected from four proficiency levels within the Yonsei English Learner Corpus, while the Louvain Corpus of Native English Essays served as a benchmark reference. A set of 35 target SNs was identified, with non-SN usages rigorously filtered out. The analysis focuses on SN frequency, various complexity levels of modifications, and contextual usage across the different corpora. Findings suggest that second language (L2) learners heavily rely on a restricted set of SNs, with increased proficiency correlating to a higher frequency of the remaining SNs. The modification of SNs shows a developmental progression, particularly through the use of prepositional phrases, which enhance both clarity and conciseness in writing. Furthermore, the use of attributive adjectives, the most basic level of modifiers, increases with proficiency but leads to excessive modifier usage in learner data. A close look at the contexts of SN usage reveals patterns instrumental for academic writing, underscoring the importance of integrating these insights into English for Academic Purposes (EAP) pedagogy.