Revisiting Apoplastic Auxin Signaling Mediated by AUXIN BINDING PROTEIN 1

Mingxiao Feng,김재연 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.10

It has been suggested that AUXIN BINDING PROTEIN 1 (ABP1) functions as an apoplastic auxin receptor, and is known to be involved in the post-transcriptional process, and largely independent of the already well-known SKPcullin- F-box-transport inhibitor response (TIR1) /auxin signaling F-box (AFB) (SCFTIR1/AFB) pathway. In the past 10 years, several key components downstream of ABP1 have been reported. After perceiving the auxin signal, ABP1 interacts, directly or indirectly, with plasma membrane (PM)-localized transmembrane proteins, transmembrane kinase (TMK) or SPIKE1 (SPK1), or other unidentified proteins, which transfer the signal into the cell to the Rho of plants (ROP). ROPs interact with their effectors, such as the ROP interactive CRIB motif-containing protein (RIC), to regulate the endocytosis/exocytosis of the auxin efflux carrier PIN-FORMED (PIN) proteins to mediate polar auxin transport across the PM. Additionally, ABP1 is a negative regulator of the traditional SCFTIR1/AFB auxin signaling pathway. However, Gao et al. (2015) very recently reported that ABP1 is not a key component in auxin signaling, and the famous abp1-1 and abp1-5 mutant Arabidopsis lines are being called into question because of possible additional mutantion sites, making it necessary to reevaluate ABP1. In this review, we will provide a brief overview of the history of ABP1 research.

Wheat Straw Modified with Palmitic Acid as an Efficient Oil Spill Adsorbent

Mingxiao Tang,Rui Zhang,Yuewu Pu 한국섬유공학회 2018 Fibers and polymers Vol.19 No.5

Raw wheat straw was modified to obtain a new oil absorption material, using palmitic acid as the esterifying agent in dimethyl sulfoxide (DMSO) without additional catalysts. The oil absorbency of the esterified wheat straw material towards 0# diesel reached 24.31 g/g under the following optimum reaction conditions: pretreated wheat straw: palmitic acid weight ratio of 1:10, reaction temperature of 90 oC, and reaction time of 3 h. The chemical composition and structure of wheat straw fiber before and after esterification reaction were compared using Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and scanning electron microscopy (SEM) methods. The results indicate that the esterification reaction was carried out smoothly, and the wheat straw afterwards had lower crystallinity and a rougher surface with more irregular folds. Additionally, the oil sorption capacity, selectivity, and absorption kinetics of the esterified wheat straw were also investigated. The esterified wheat straw showed good potential for converting agricultural residues into efficient cellulosic oil sorbents.

LQR Pendulation Reduction Control of Ship-Mounted Crane Based on Improved Grey Wolf Optimization Algorithm

Mingxiao Sun,Changyu Ji,Tiantian Luan,Nan Wang 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.3

The poor adaptability matrix of traditional LQR controller causes the problems of large payload swing and slow response for ship-mounted cranes during operation. To solve such problems, an LQR controller based on an improved grey wolf optimization algorithm (IGWO-LQR) is proposed. Firstly, the dynamics model of ship-mounted crane is constructed, the pendulum reduction problem is transformed into the LQR quadratic performance index problem, and IGWO is used to optimize the weight matrix. At the same time, the RBF neural network is applied to compensate for the non-linear wave disturbances in the system. Finally, the pendulum reduction efficiency of the controller under different parameters and conditions is verified by numerical simulation. Compared with the traditional LQR controller, the simulation results show that the control accuracy of the IGWO-LQR controller is improved by about 5%, and the response speed is improved by about 5–10 s. This method can significantly reduce the payload swing and improve work efficiency.

Revisiting Apoplastic Auxin Signaling Mediated by AUXIN BINDING PROTEIN 1

Feng, Mingxiao,Kim, Jae-Yean Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.10

It has been suggested that AUXIN BINDING PROTEIN 1 (ABP1) functions as an apoplastic auxin receptor, and is known to be involved in the post-transcriptional process, and largely independent of the already well-known SKP-cullin-F-box-transport inhibitor response (TIR1) /auxin signaling F-box (AFB) ($SCF^{TIR1/AFB}$) pathway. In the past 10 years, several key components downstream of ABP1 have been reported. After perceiving the auxin signal, ABP1 interacts, directly or indirectly, with plasma membrane (PM)-localized transmembrane proteins, transmembrane kinase (TMK) or SPIKE1 (SPK1), or other unidentified proteins, which transfer the signal into the cell to the Rho of plants (ROP). ROPs interact with their effectors, such as the ROP interactive CRIB motif-containing protein (RIC), to regulate the endocytosis/exocytosis of the auxin efflux carrier PIN-FORMED (PIN) proteins to mediate polar auxin transport across the PM. Additionally, ABP1 is a negative regulator of the traditional $SCF^{TIR1/AFB}$ auxin signaling pathway. However, Gao et al. (2015) very recently reported that ABP1 is not a key component in auxin signaling, and the famous abp1-1 and abp1-5 mutant Arabidopsis lines are being called into question because of possible additional mutantion sites, making it necessary to reevaluate ABP1. In this review, we will provide a brief overview of the history of ABP1 research.

Control System Design of Anti-rolling Tank Swing Bench Using BP Neural Network PID Based on LabVIEW

Lihua Liang,Mingxiao Sun,Songtao Zhang,Yu Wen,Peng Zhao,Jia Yuan 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.6

The anti-rolling tank swing bench is a typical hydraulic position servo system. Its function is to simulate the ship roll motion and to verify the performance of the anti-rolling tank. Generally, the PID control method can achieve an ideal control effect. But the uncertainties, such as the couplings and the nonlinearty of the swing bench, make it difficult to establish the precise mathematical model of system. During experiments, the different disturbance torques are generated according to the different dimension scales of the anti-rolling tanks. Changes of the natural characteristics of the system are caused by changes of load, and the fixed parameters of PID controller are difficult to continue to ensure a good control effect. In order to reduce the influences caused by the factors mentioned above, a BP neural network PID control system based on virtual instrument LabVIEW is designed. By simulating the responses of the system in real time control, the verification tests using actual system are avoided. Finally, the experiments of swing bench are carried out. The results show that the controller can ensure the control accuracy and stability. The parameters tuning process efficiency is improved. The operation is convenient. The data acquisition process is simple and practical.

Pedestrian Detection Algorithm Combining HOG and SLBP

Aili Wang,Mingxiao Wang,Jitao Zhang,Yuji Iwahori,Bo Wang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

In order to solve the problem of pedestrian detection performance, the described operator was improved. In this paper, semantic local binary pattern (SLBP) and histogram of oriented gradient (HOG) are combined as new feature operator. This feature method would enrich the information and enhance the detection performance. And then histogram intersection kernel support vector machine (HIKSVM) classifier is trained by the augment feature. Because the time cost is too large by the conventional SVM. HIKSVM could make up this drawback, and significantly reduce the training time. The experiments on the INRIA pedestrian dataset show that the method obtained significant improvement in accuracy comparing to HOG descriptors.

Infuence of Dynamic Characteristics of Induction Motor on Voltage Sag Caused by Short Circuit Fault

Jiming Chen,Hai Zuo,Qianyu Yu,Lizhi Qi,Mingxiao Zhu 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

Voltage sag is a signifcant problem that afects the quality of power, and some researches have been performed on this theme. The characteristic of load is an important factor afecting the voltage sag, which is rarely studied. This paper investigates the infuence of dynamic characteristics of induction motor on voltage sag caused by short circuit fault. The analytic expression of induction electromotive force is derived by space vector method. The mechanism of amplitude and waveform changes caused by dynamic load characteristics of induction motor are analyzed. The results indicate that the voltage at the load terminal of the induction motor is higher than that at the static load during the sag. Besides, the voltage sag waveform is not in rectangular shape. The static load terminal voltage returns to normal value quickly, while the voltage at the load terminal of the induction motor needs about 200 ms after the fault is removed. Induction motors can reduce the magnitude of voltage sags caused by short circuit faults, but will extend the duration. At the same time, the bigger the induction motor capacity is, the longer the voltage recovery time after sag is.

Highly selective fluorescence imaging of zinc distribution in HeLa cells and <i>Arabidopsis</i> using a naphthalene-based fluorescent probe

Lee, Ji Ha,Lee, Jin Hyeok,Jung, Sung Ho,Hyun, Tae Kyung,Feng, Mingxiao,Kim, Jae-Yean,Lee, Jae-Hong,Lee, Hoyeon,Kim, Jong Seung,Kang, Chulhun,Kwon, Ki-Young,Jung, Jong Hwa The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.35

<P>2-(<I>N</I>,<I>N</I>-Dimethylamino)naphthalene-based probe <B>1</B> was found to exhibit a dramatic enhancement in fluorescence upon addition of Zn<SUP>2+</SUP>, but not with any other metal ions. Probe <B>1</B> as a chemoprobe enabled high-resolution fluorescence imaging of zinc ions in HeLa cells and <I>Arabidopsis</I>.</P> <P>Graphic Abstract</P><P>2-(<I>N</I>,<I>N</I>-Dimethylamino)naphthalene-based probe <B>1</B> was found to exhibit a dramatic enhancement in fluorescence upon addition of Zn<SUP>2+</SUP>, but not with any other metal ions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc01364j'> </P>

Drug-loaded microbubble delivery system to enhance PD-L1 blockade immunotherapy with remodeling immune microenvironment

Zheng Jun,Huang Ju,Zhang Liang,Wang Mengna,Xu Lihong,Dou Xiaoyun,Leng Xiaojing,Fang Mingxiao,Sun Yang,Wang Zhigang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Although programmed cell death protein 1 (PD-1)/ programmed cell death-ligand protein 1 (PD-L1) checkpoint blockade immunotherapy demonstrates great promise in cancer treatment, poor infiltration of T cells resulted from tumor immunosuppressive microenvironment (TIME) and insufficient accumulation of anti-PD-L1 (αPD-L1) in tumor sites diminish the immune response. Herein, we reported a drug-loaded microbubble delivery system to overcome these obstacles and enhance PD-L1 blockade immunotherapy.Docetaxel (DTX) and imiquimod (R837)-loaded microbubbles (RD@MBs) were synthesized via a typical rotary evaporation method combined with mechanical oscillation. The targeted release of drugs was achieved by using the directional "bursting" capability of ultrasound-targeted microbubble destruction (UTMD) technology. The antitumor immune response by RD@MBs combining αPD-L1 were evaluated on 4T1 and CT26 tumor models.The dying tumor cells induced by DTX release tumor-associated antigens (TAAs), together with R837, promoted the activation, proliferation and recruitment of T cells. Besides, UTMD technology and DTX enhanced the accumulation of αPD-L1 in tumor sites. Moreover, RD@MBs remolded TIME, including the polarization of M2-phenotype tumor-associated macrophages (TAMs) to M1-phenotype, and reduction of myeloid-derived suppressor cells (MDSCs). The RD@MBs + αPD-L1 synergistic therapy not only effectively inhibited the growth of primary tumors, but also significantly inhibited the mimic distant tumors as well as lung metastases.PD-L1 blockade immunotherapy was enhanced by RD@MBs delivery system.

FXR Regulates Intestinal Cancer Stem Cell Proliferation

Fu, Ting,Coulter, Sally,Yoshihara, Eiji,Oh, Tae Gyu,Fang, Sungsoon,Cayabyab, Fritz,Zhu, Qiyun,Zhang, Tong,Leblanc, Mathias,Liu, Sihao,He, Mingxiao,Waizenegger, Wanda,Gasser, Emanuel,Schnabl, Bernd,Atk Elsevier 2019 Cell Vol.176 No.5

<P><B>Summary</B></P> <P>Increased levels of intestinal bile acids (BAs) are a risk factor for colorectal cancer (CRC). Here, we show that the convergence of dietary factors (high-fat diet) and dysregulated WNT signaling (APC mutation) alters BA profiles to drive malignant transformations in Lgr5-expressing (Lgr5<SUP>+</SUP>) cancer stem cells and promote an adenoma-to-adenocarcinoma progression. Mechanistically, we show that BAs that antagonize intestinal farnesoid X receptor (FXR) function, including tauro-β-muricholic acid (T-βMCA) and deoxycholic acid (DCA), induce proliferation and DNA damage in Lgr5<SUP>+</SUP> cells. Conversely, selective activation of intestinal FXR can restrict abnormal Lgr5<SUP>+</SUP> cell growth and curtail CRC progression. This unexpected role for FXR in coordinating intestinal self-renewal with BA levels implicates FXR as a potential therapeutic target for CRC.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genetic and dietary risk factors for colorectal cancer converge on the BA-FXR axis </LI> <LI> FXR controls proliferating Lgr5<SUP>+</SUP> intestinal stem cells </LI> <LI> FXR agonists curtail colorectal cancer progression </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>