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Iron Depletion Decreases ROS in Porcine Early Embryos Developing In Vitro
Ming-Hui Zhao,Shuang Liang,Jung-Woo Kwon,Seon-Hyang Kim,Nam-Hyung Kim,Xiang-Shun Cui 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
Iron is an important element which takes part in several biological processes. The role of iron in porcine oocyte maturation and early embryo development was still unknown. In the present study, iron was depleted by deferoxamine (DFM), a specific iron chelator, during in vitro maturation (IVM) and in vitro culture (IVC) period respectively. Results showed that, oocytes were matured with 1 μM DFM for 44 h followed by parthenogenetic activation (PA) displayed higher (p<0.05) blastocyst rate compared to without DFM in IVM medium. Another hand, supplemented with 0.5 μM DFM to the IVC medium for 7 days enhanced blastocyst formation both in PA and in vitro fertilization (IVF) groups (p<0.05). Although there was no difference in total cell number in IVF-derived blastocysts between control and iron depleted group, apoptotic index was significantly (p<0.05) decreased following iron depletion. ROS content in MII oocyte and blastocyst was significantly (p<0.05) decreased after iron depletion. For clarify the mechanism of the phenomenon, expressions of apoptotic- and ROS-related genes in the blastocyst were checked. Iron depletion reduced the expression of Caspase 3 and increased Bcl-xL in blastocyst (p<0.05), but there was no significanly differenc in m-RNA expression of Nox4. Mitochondrial membrane potential was increased both in MII oocytes and blastocysts after DFM treatment. In conclusion, iron depletion decreased ROS in oocytes and embryos developing in vitro, further enhanced mitochondrial activity and reduced the apoptosis in blastosyst, resulted in enhancement of the developmental capacity in porcine early embryos.
Ming-Hui Wang,Kenko Uchida 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10
This paper investigates the consensus problem of the second-order multi-agent systems with communication channel constraint on signal amplitude. Communication delays as one kind of channel constraints in the second-order multi-agent systems have been discussed in the recent literature while in this paper consensus problem of second-order multi-agent systems with communication channel constraint on signal amplitude is investigated. A state-dependent switching parameter is introduced into the consensus protocol. A sufficient condition is obtained for the consensus problem of the second-order multi-agent systems with communication channel constraint based on our early work. A simple example is also given to verify the effectiveness of the proposed theorem.
Intelligent fuzzy weighted input estimation method for the input force on the plate structure
Ming-Hui Lee,Tsung-Chien Chen 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.1
The innovative intelligent fuzzy weighted input estimation method which efficiently and robustly estimates the unknown time-varying input force in on-line is presented in this paper. The algorithm includes the Kalman Filter (KF) and the recursive least square estimator (RLSE), which is weighted by the fuzzy weighting factor proposed based on the fuzzy logic inference system. To directly synthesize the Kalman filter with the estimator, this work presents an efficient robust forgetting zone, which is capable of providing a reasonable compromise between the tracking capability and the flexibility against noises. The capability of this inverse method are demonstrated in the input force estimation cases of the plate structure system. The proposed algorithm is further compared by alternating bwtween the constant and adaptive weighting factors. The results show that this method has the properties of faster convergence in the initial response, better target tracking capability, and more effective noise and measurement bias reduction.
Nonlinear structural system wind load input estimation using the extended inverse method
Ming-Hui Lee 한국풍공학회 2013 한국풍공학회지 Vol.17 No.4
This study develops an extended inverse input estimation algorithm with intelligent adaptive fuzzy weighting to effectively estimate the unknown input wind load of nonlinear structural systems. This algorithm combines the extended Kalman filter and recursive least squares estimator with intelligent adaptive fuzzy weighting. This study investigated the unknown input wind load applied on a tower structural system. Nonlinear characteristics will exist in various structural systems. The nonlinear characteristics are particularly more obvious when applying larger input wind load. Numerical simulation cases involving different input wind load types are studied in this paper. The simulation results verify the nonlinear characteristics of the structural system. This algorithm is effective in estimating unknown input wind loads.
Ming-Hui Lee 국제구조공학회 2013 Smart Structures and Systems, An International Jou Vol.12 No.2
This study proposes an innovative control approach to suppress the responses of a beam structural system under moving forces. The proposed control algorithm is a synthesis of the adaptive input estimation method (AIEM) and linear quadratic Gaussian (LQG) controller. Using the synthesis algorithm the moving forces can be estimated using AIEM while the LQG controller offers proper control forces to effectively suppress the beam structural system responses. Active control numerical simulations of the beam structural system are performed to evaluate the feasibility and effectiveness of the proposed control technique. The numerical simulation results show that the proposed method has more robust active control performance than the conventional LQG method.
Ming-Hui Lee 국제구조공학회 2012 Smart Structures and Systems, An International Jou Vol.10 No.1
This study proposes an active control method to suppress beam-rotating machinery system vibrations. The present control method is a combination of the fuzzy input estimation method (FIEM) and linear quadratic Gaussian problem (LQG) algorithms. The FIEM can estimate the unknown input and optimal states by measuring the dynamic displacement, the optimal estimated states into the feedback control; thereby obtaining the optimal control force for a random linear system. Active vibration control of a beam-rotating machinery system is performed to verify the feasibility and effectiveness of the proposed algorithm. The simulation results demonstrate that the proposed method can suppress vibrations in a beam-machine system more efficiently than the conventional LQG method.
Function of Global Regulator CodY in Bacillus thuringiensis BMB171 by Comparative Proteomic Analysis
( Ming Xia Qi ),( Fei Mei ),( Hui Wang ),( Ming Sun ),( Ge Jiao Wang ),( Ziniu Yu ),( Yeon Ho Je ),( Ming Shun Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2
CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY-, named BMB171cry1Ac and BMB171codY-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.
Cluster Consensus of Multi-agent Systems with Communication Delay (ICCAS 2013)
Ming-Hui Wang,Kenko Uchida 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
Cluster consensus problem of the multi-agent systems with communication delay is studied in this paper. We consider the multi-agent systems with a special Laplacian matrix as its system matrix. For two types of protocols with time-delay, our work shows that the multi-agent systems with communication delays can reach cluster consensus. Some numerical simulations are given to verify the effectiveness of the theoretical results.