Differentially Expressed Genes under Cold Acclimation in Physcomitrella patens

( Ming Ming Sun ),( Lin Hui Li ),( Hua Xie ),( Rong Cai Ma ),( Yi Kun He ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.6

Scrotal Sparganosis Mimicking Scrotal Teratoma in an Infant

Yi-Ming Zhao,Hao-Chuan Zhang,Zhong-Rong Li,Hai-Yan Zhang 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.5

Tenofovir Alafenamide for Chronic Hepatitis B Patients with Advanced Fibrosis and Partial Virologic Responses to Oral Nucleos(T)Ide Analogues- Interim Report

( Ming-lung Yu ),( Ming-lun Yeh ),( Chi-yi Chen ),( Pin-nan Cheng ),( Ming-jong Bair ),( Jyh-jou Chen ),( Ching-chu Lo ),( Chi-ming Tai ),( Ching-yang Tsai ),( Kuo-chih Tseng ),( Chien-hung Chen ),( C 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Insufficient data regarding the treatment strategy for partial response to nucleot(s)ide analogue (NUC) raised the aim of investigating tenofovir alafenamide (TAF) switching for chronic hepatitis B (CHB) patients with advanced fibrosis and partial response to other NUCs. Methods: CHB patients with advanced fibrosis (stage 3 or 4) and under NUC (except TAF) therapy with detectable hepatitis B virus (HBV) DNA for >52 weeks are enrolled to TAF 25 mg/day for 96 weeks. The objectives are viral suppression, alanine aminotransferase (ALT) normalization and safety. Results: From Feb. 2019, 34 patients, including 21 (61.8%) with entecavir, 10 (29.4%) TDF and 3 (8.8%) lamivudine or adefovir, were enrolled (15 [44.1%] male, median 53 years). The fibroscan demonstrated a mean of 10.5 kPa (7 [20.6%] cirrhotic). Sixteen (47.1%) patients were HBV e antigen positive, seven (20.6%) had YMDD mutation. The median HBV DNA level declined from 68.5 IU/mL at enrollment to 27.0 IU/mL at 4^th week, and undetectable at 12^th, 24^th, 36^th week, respectively, after TAF switching, with undetectable HBV DNA in 14/34 (41.2%), 17/33 (51.5%), 15/25 (60.0%), and 9/15 (60.0%) patients and rate of ALT normalization (≤40 U/L) of 85.3%, 85.3%, 84.8%, 92.0%, and 80.0%, respectively, after TAF switching. (figure 1) Two patients experienced transient virological breakthrough and another one developed at the final time follow up. Serum creatinine and eGFR levels were stable after TAF switching (figure 1). Two patients early terminated including one at 12^th week due to personal reason, and another one accidently died at 20^th week due to acute heart attack. Others suffered only mild degrees of adverse events which were considered unrelated to treatment. Conclusions: The preliminary results demonstrated the TAF switching is effective and safe in viral suppression for CHB patients with advanced fibrosis and partial virologic responses to other NUCs.

Exploring the Key Genes and Pathways of Osteoarthritis in Knee Cartilage in a Rat Model Using Gene Expression Profiling

Yi-Ming Ren,Xin Zhao,Tao Yang,Yuan-Hui Duan,Yun-Bo Sun,Wen-Jun Zhao,Meng-Qiang Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.6

Purpose: To compare differentially expressed genes (DEGs) mediating osteoarthritis (OA) in knee cartilage and in normal kneecartilage in a rat model of OA and to identify their impact on molecular pathways associated with OA. Materials and Methods: A gene expression profile was downloaded from the Gene Expression Omnibus database. Analysis ofDEGs was carried out using GEO2R. Enrichment analyses were performed on the Gene Ontology (GO) and Kyoto Encyclopedia ofGenes and Genomes pathway using the Search Tool for the Retrieval of Interacting Genes database (http://www.string-db.org/). Subsequently, the regulatory interaction network of OA-associated genes was visualized using Cytoscape software (version 3.4.0;www.cytoscape.org). Results: In the gene expression profile GSE103416, a total of 99 DEGs were identified. Among them, 76 DEGs (76.77%) were overexpressed,and the remaining 23 DEGs (23.23%) were underexpressed. GO and pathway enrichment analyses of target geneswere performed. Using gene-gene interaction network analysis, relevant core genes, including MET, UBB, GNAI3, and GNA13,were shown to hold a potential relationship with the development of OA in cartilage. Using quantitative real-time PCR, the Gna13/cGMP-PKG signaling pathway was identified as a potential research target for therapy and for further understanding the developmentof OA. Conclusion: The results of the present study provide a comprehensive understanding of the roles of DEGs in knee cartilage in relationto the development of OA.

Sofosbuvir/Ledipasvir in the Treatment of Chronic Hepatitis C - A Subgroup Analysis from A Nationwide Real-World HCV Registry Program (TACR) in Taiwan

( Ming-Lung Yu ),( Chi-Yi Chen ),( Kuo-Chih Tseng ),( Ching-Chu Lo ),( Pin-Nan Cheng ),( Cheng-Yuan Peng ),( Ming-Jong Bair ),( Chih-Lang Lin ),( Chi-Ming Tai ),( Chi-Chieh Yang ),( Chih-Wen Lin ),( C 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: TASL HCV Registry (TACR) is a nationwide registry program organized and supervised by Taiwan Association for the Study of the Liver (TASL), which aims to setup the database and biobank of patients with chronic hepatitis C (CHC) in Taiwan. The present study aimed to evaluate the treatment outcome of sofosbuvir (SOF)/ledipasvir (LDV) in Taiwanese CHC patients in TACR. Methods: By May 2020, 19 tertiary hospitals, 23 community hospitals and one primary care clinic join the TACR program. The baseline characteristics, prior liver and non-liver related medical history, DAA regimens, laboratory results, treatment course and outcome were recorded. The primary objective was sustained virological response, defined as undetectable HCV RNA 3 months after end-of-treatment (SVR12). Results: A total of 4742 SOF/LDV+ ribavirin treated CHC patients with available SVR12 data from 39 sites were enrolled in the current analysis. The mean age was 61.3 years, and female accounted for 54.8% of the population. The dominant viral genotypes were GT1b (52.6%) and GT2 (35.6%). 1354 (28.6%) patients had liver cirrhosis, including 156 (3.3%) with liver decompensation, 552 (11.6%) had preexisting hepatocellular carcinoma (HCC) before DAAs treatment and 413 (8.7%) had hepatitis B virus dual infections. The overall SVR12 rate was 98.5%, with 98.5%, 98.2%, 99.7% and 98.6% in treatment- naïve non-cirrhotics, treatment-naïve cirrhotics, treatment- experienced non-cirrhotics and treatment-experienced cirrhotics patients, respectively. While patients were stratified by HCV genotype, the SVR12 was 98.5%, 98.4% and 98.5% among those with GT1, GT2 and GT6 infection, respectively. The strongest factor independent associated with treatment failure was DAA adherence < 60% (odds ratio [OR]/95% confidence intervals [CI]: 125.4/25.7-612.4, P<0.0001), followed by active HCC (OR/CI: 6.20/2.57-14.97, P<0.0001), HIV co-infection (OR/CI: 3.01/1.14-7.92, P=0.026), and male gender (OR/ CI: 1.85/1.09-3.13, P=0.023). The eGFR decreased significantly at the end of treatment (EOT) (89.3 ml/min/1.73㎡ vs. 93.2 ml/min/1.73㎡, P< 0.001) and remained stable 3 months after EOT (89.3 ml/min/1.73㎡). However, the decreased eGFR was observed only in patients whose baseline eGFR > 90 ml/ min/1.73㎡. Instead, patients with chronic kidney diseases whose pretreatment eGFR < 60 ml/min/1.73㎡ had improved eGFR after SOF/LDV. Conclusions: SOF/LDV is highly effective in treating CHC patients in real-world setting of Taiwan. The satisfactory result could be explicitly generalized to patients with different viral genotypes and liver disease severities.

20, 30대 중국 소비자의 생명보험 가입 의도 -국가에 대한 인식 및 집단주의 성향에 따른 유형별 특성과 코로나19 불안의 조절 효과-

Ming-Yi Zhu,천혜정 한중사회과학학회 2023 한중사회과학연구 Vol.21 No.2

The purpose of this study is to categorize Chinese consumers in their 20s and 30s according to their perception of the state and their collectivism tendency, and to empirically analyze how perception of the state, collectivism tendency, attitudes toward insurance, and preparation for old age affect insurance purchasing intention. In addition, it also analyzes the moderating effect of anxiety caused by covid-19. To this end, an online survey was conducted on Chinese consumers in their 20s and 30s living in Shanghai, China, and a total of 500 questionnaires were used for analysis. The main findings are as follows. First, according to the perception of the state and the tendency of collectivism, Chinese consumers in their 20s and 30s were categorized into three groups. Specifically, the three groups are “individualist critics” who are negative and critical of the state and have the lowest collectivism tendency, “moderate neutrals” who have a moderate attitude toward the state and collectivitism, and “collectivist optimists” who are positive and optimistic about the state and have the highest collectivism tendency. Second, there were no significant demographic differences among the three groups, but significant differences were found in the attitude toward insurance, the degree of preparation for old age, and the level of anxiety about covid-19. Third, attitude toward insurance, perception of the state, and collectivism tendency were found to affect the insurance purchasing intention among 3 groups, respectively, and covid-19 anxiety has a moderating effect to attitude toward insurance, perception of the state, and collectivism tendency for each group. Based on these results, the relationship between covid-19 anxiety and insurance purchasing intention was discussed in various ways along with diversity within the Chinese consumers in their 20s and 30s. 본 연구의 목적은 국가에 대한 인식 및 집단주의 성향에 따라 20, 30대 중국 소비자를 유형화하고 집단별 보험 가입 의도에 영향을 미치는 요인을 실증적으로 살펴보고, 이 과정에서 코로나19로 인한 불안이 어떠한 조절 효과를 가지는지 분석하는 것이다. 이를 위해 중국 상하이시에 거주하는 20, 30대 중국 소비자를 대상으로 온라인 설문조사를 실시했으며 총 500부의 자료를 분석에 활용했다. 주요 연구 결과는 다음과 같다. 첫째, 국가에 대한 인식 및 집단주의 성향에 따라 20, 30대 중국 소비자를 유형화한결과 3개의 집단으로 분류되었다. 구체적으로 이 세 집단은 국가에 대해 부정적이고비판적인 태도를 가지면서 집단주의 성향도 가장 낮은 “개인주의적 비판론자”, 국가에대해 중도적인 온건한 태도를 가지면서 집단주의 성향도 중간 정도 수준을 보이는 “온건한 중립자”, 그리고 국가에 대해 긍정적이고 낙관적인 태도를 가지면서 집단주의 성향도 가장 높은 “집단주의적 낙관론자”이다. 둘째, 세 집단간 유의미한 인구통계학적차이는 나타나지 않았지만, 보험에 대한 태도, 노후 준비 정도, 그리고 코로나19 불안수준에서는 유의미한 차이가 나타났다. 셋째, 집단별 보험 가입 의도에 영향을 미치는요인과 이 과정에서 코로나19 불안의 조절 효과를 분석한 결과, 개인주의적 회의론자집단은 보험에 대한 태도, 온건한 중립자 집단은 국가에 대한 인식, 그리고 집단주의적 낙관론자는 집단주의 성향이 유의미한 요인으로 확인되었으며 각각의 독립변수와코로나19 불안과의 상호작용 또한 유의미한 것으로 나타났다. 이러한 결과를 바탕으로20, 30대 중국 소비자 집단 내부의 다양성과 함께 코로나19 불안과 보험 가입 의도와의 관계를 다각적으로 논의하였다.

Transarterial Chemoembolization Using Gelatin Sponges or Microspheres Plus Lipiodol-Doxorubicin versus Doxorubicin-Loaded Beads for the Treatment of Hepatocellular Carcinoma

Yi-Sheng Liu,Ming-Ching Ou,Yi-Shan Tsai,Xi-Zhang Lin,Chien-Kuo Wang,Hong-Ming Tsai,Ming-Tsung Chuang 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.1

To retrospectively compare treatment of hepatocellular carcinoma (HCC) with transarterial chemoembolization (TACE) using gelatin sponges or microspheres plus lipiodol-doxorubicin vs. doxorubicin-loaded drug-eluting beads (DEB). A total of 158 patients with HCC received TACE from November 2010 to November 2011 were enrolled in this study, including 64 (40.5%) received TACE with lipiodol-doxorubicin and gelatin sponges (group A), 41 (25.9%) received TACE with lipiodol-doxorubicin and microspheres (group B), and 53 (33.5%) received TACE with doxorubicin-loaded DEB (group C). Tumor response and adverse events (AEs) were evaluated. No significant difference was found at baseline among the three groups. The doxorubicin dosage in group C wassignificantly (p < 0.001) higher compared to the dose used in groups A or B (median, 50 mg vs. 31 mg or 25 mg). Significantly (p < 0.001) more patients in group C achieved complete response compared to those in groups A or B (32.1% vs. 6.3% or 2.4%). Significantly (p < 0.001) less patients in group C had progressive disease compared to those in groups A or B (34.0% vs. 57.8% or 68.3%). Minor AEs were more common in groups A and B compared to group C, with rates of 54.7%, 34.1%, and 5.7%, respectively. In patients with HCC, TACE with DEB offers better safety and efficacy profiles compared to either TACE with gelatin sponges or TACE with microspheres.

Anti-tumor and Anti-inflammatory Activity of the Methanol Extracts from Adlay Bran

Ming-Yi Lee,Shu-Hsien Tsai,Yueh-Hsiung Kuo,Wenchang Chiang 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.6

Adlay bran is a waste product previously thought to have no commercial value. Its methanolic extract was fractionated using n-hexane (ABM-Hex), ethyl acetate (ABM-EtOAc), 1-butanol (ABM-BuOH), and water (ABM-H₂O). The ABM-EtOAc fraction exhibited a strongest inhibition against growth of human lung cancer cell A549 and human colorectal carcinoma cells HT-29 and COLO 205. Inhibition of cell cycle progression at G?/G₁ transition, increase of cells at the sub-G1 phase, and DNA ladders were observed in cells treated with ABM-EtOAc. The ABM-BuOH fraction showed the strongest inhibition of proinflammatory cytokines tumor necrosis factor (TNF)-α and interlukin (IL)-1β in stimulated RAW 264.7 macrophages. Further, ABM-EtOAc and ABM-BuOH inhibited cyclooxygenase (COX)-2 expression in A549 and HT-29 carcinoma cells, while COX-1 expression was not affected. These results reveal that both ABM-EtOAc and ABM-BuOH may aid the prevention of cancers and the applications in cancer chemotherapy.

Modified Blumgart Method for Pancreaticoduodenectomy

Yi-Ming Shyr,Shin-E Wang 한국간담췌외과학회 2017 한국간담췌외과학회 학술대회지 Vol.2017 No.3

A Beacon-Based Trust Management System for Enhancing User Centric Location Privacy in VANETs

Yi-Ming Chen,Yu-Chih Wei 한국통신학회 2013 Journal of communications and networks Vol.15 No.2

In recent years, more and more researches have been focusing on trust management of vehicle ad-hoc networks (VANETs)for improving the safety of vehicles. However, in these researches,little attention has been paid to the location privacy due to the natural conflict between trust and anonymity, which is the basic protection of privacy. Although traffic safety remains the most crucial issue in VANETs, location privacy can be just as important for drivers, and neither can be ignored. In this paper, we propose a beacon-based trust management system, called BTM, that aims to thwart internal attackers from sending false messages in privacy-enhanced VANETs. To evaluate the reliability and performance of the proposed system, we conducted a set of simulations under alteration attacks, bogus message attacks, and message suppression attacks. The simulation results show that the proposed system is highly resilient to adversarial attacks, whether it is under a fixed silent period or random silent period location privacyenhancement scheme.