http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Chih-Jung Yao,Jyh-Ming Chow,Shuang-En Chuang,Chia-Lun Chang,Ming-De Yan,Hsin-Lun Lee,I-Chun Lai,Pei-Chun Lin,Gi-Ming Lai 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cancer cells to investigate the induction of Forkhead Class box O3a (FOXO3a) and autophagy. Methods: Cell viability was determined by sulforhodamine B staining. Apoptosis and cell cycle distribution were analyzed using flow cytometry. The changes of protein levels were determined using Western blot analysis. Autophagy induction was monitored by the formation of acidic vesicular organelles stained with acridine orange. Results: KG-135 effectively arrested the cells in G1 phase with limited apoptosis. Accordingly, a decrease of cyclin-dependent kinase-4, cyclin-dependent kinase-6, cyclin D1, and phospho-retinoblastoma protein, and an increase of p27 and p18 proteins were observed. Intriguingly, KG-135 increased the tumor suppressor FOXO3a and induced the accumulation of autophagy hallmark LC3-II and acidic vesicular organelles without an increase of the upstream marker Beclin-1. Unconventionally, the autophagy adaptor protein p62 (sequestosome 1) was increased rather than decreased. Blockade of autophagy by hydroxychloroquine dramatically potentiated KG-135-induced FOXO3a and its downstream (FasL) ligand accompanied by the cleavage of caspase-8. Meanwhile, the decrease of Bcl-2 and survivin, as well as the cleavage of caspase-9, were also drastically enhanced, resulting in massive apoptosis. Conclusion: Besides arresting the cells in G1 phase, KG-135 increased FOXO3a and induced an unconventional autophagy in A549 cells. Both the KG-135-activated extrinsic FOXO3a/FasL/caspase-8 and intrinsic caspase-9 apoptotic pathways were potentiated by blockade of autophagy. Combination of KG- 135 and autophagy inhibitor may be a novel strategy as an integrative treatment for cancers.
Yao, Chih-Jung,Chow, Jyh-Ming,Chuang, Shuang-En,Chang, Chia-Lun,Yan, Ming-De,Lee, Hsin-Lun,Lai, I-Chun,Lin, Pei-Chun,Lai, Gi-Ming The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3
Background: KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cancer cells to investigate the induction of Forkhead Class box O3a (FOXO3a) and autophagy. Methods: Cell viability was determined by sulforhodamine B staining. Apoptosis and cell cycle distribution were analyzed using flow cytometry. The changes of protein levels were determined using Western blot analysis. Autophagy induction was monitored by the formation of acidic vesicular organelles stained with acridine orange. Results: KG-135 effectively arrested the cells in G1 phase with limited apoptosis. Accordingly, a decrease of cyclin-dependent kinase-4, cyclin-dependent kinase-6, cyclin D1, and phospho-retinoblastoma protein, and an increase of p27 and p18 proteins were observed. Intriguingly, KG-135 increased the tumor suppressor FOXO3a and induced the accumulation of autophagy hallmark LC3-II and acidic vesicular organelles without an increase of the upstream marker Beclin-1. Unconventionally, the autophagy adaptor protein p62 (sequestosome 1) was increased rather than decreased. Blockade of autophagy by hydroxychloroquine dramatically potentiated KG-135-induced FOXO3a and its downstream (FasL) ligand accompanied by the cleavage of caspase-8. Meanwhile, the decrease of Bcl-2 and survivin, as well as the cleavage of caspase-9, were also drastically enhanced, resulting in massive apoptosis. Conclusion: Besides arresting the cells in G1 phase, KG-135 increased FOXO3a and induced an unconventional autophagy in A549 cells. Both the KG-135-activated extrinsic FOXO3a/FasL/caspase-8 and intrinsic caspase-9 apoptotic pathways were potentiated by blockade of autophagy. Combination of KG-135 and autophagy inhibitor may be a novel strategy as an integrative treatment for cancers.
Two New Metabolites with Cytotoxicities from Deep-Sea Fungus, Aspergillus sydowi YH11-2
De-Hai Li,Sheng-Xin Cai,Li Tian,Zhen-Jian Lin,Tian-Jiao Zhu,Yu-Chun Fang,Pei-Pei Liu,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.9
Two new compounds, 2, 3, 5-trimethyl-6-(3-oxobutan-2-yl)-4H-pyran-4-one (1) and (2R)-2, 3- dihydro-7-hydroxy-6, 8-dimethyl-2-[(E)-prop-1-enyl] chromen-4-one (2), together with six known compounds (3-8), were isolated from a deep-sea fungus, identified as Aspergillus sydowi, by a bioassay-guided method. Their structures were elucidated by spectroscopic methods and the cytotoxicities were evaluated by SRB method.
Study on the Lethal Reason of Overwintering Weedy Rice Seed in Cold Rice Production Area in China
Ming-gen Wu,Jie Yang,De-liang Yang,Feng Xu,Min-jie Fu 한국잡초학회 2011 한국잡초학회 별책(학술대회 초록집) Vol.31 No.2
The outdoor trails were carried out to investigate overwintering survival rate of different weedy rice seeds in two latitude areas including Longjing city located in Jilin province and Donggang city located in Liaoning province in China, respectively. The survival rates of all weedy rice seeds which were frozen under the condition of saturated soil for 3 months are about 0%. The germination rates of weedy rice seeds overwintering in air-dried soil are more than 50%. The death of overwintering weedy rice seed in the two production areas was positively related to its dormancy characteristic, seed water content and temperature during seed frozen period.
de Luna, Mark Daniel G.,Wan, Meng-Wei,Golosinda, Lucille R.,Futalan, Cybelle M.,Lu, Ming-Chun ACS AMERICAN CHEMICAL SOCIETY 2017 ENERGY AND FUELS Vol.31 No.9
<P>Mixing-assisted oxidative desulfurization (MAOD) of model fuel that contains dibenzothiophene (DBT) using phosphotungstic acid (HPW) as a catalyst and hydrogen peroxide (H2O2) as an oxidant was evaluated. Characterization analysis of HPW shows that the average crystallite size is 82.39 nm, with a disintegrated structure and morphology. The effect of operating parameters, such as mixer speed (5000-10 000 rpm), tetraoctylammoniurn bromide [phase-transfer agent (PTA)]/HPW ratio (0.5:1-5:1), and temperature (25-60 degrees C), on the sulfur conversion of DBT was examined. Results show that the sulfur conversion increases with an increasing temperature and mixer speed and a lower amount of PTA. The highest sulfur removal and rate constant of 100.0% and 0.1528 min(-1), respectively, were attained under the following conditions: 1:1 ratio, 40 degrees C, and 10 000 rpm. The pseudo-first-order equation and Arrhenius equation were applied to determine the kinetic rate constant and activation energy of HPW in the oxidation of DBT in a MAOD system. High correlation coefficient values (R-2 >= 0.93) indicate that the pseudo-first-order equation has the goodness of fit in describing the experimental kinetic data. Moreover, the activation energy of HPW derived from the Arrhenius equation was 42.60 kJ/mol.</P>
Ming-Chun Wang,Chia-Ying Chan,Christian de Peretti,Hong-Min Chen 한국증권학회 2017 Asia-Pacific Journal of Financial Studies Vol.47 No.5
This study examines the volatility spillover effect among five index options and their underlying markets. Results show that the bidirectional volatility spillover effect and the cross-market leverage effect exist between index options and their underlying markets. Our findings confirm that the volatility spillover effect is generally outweighed by shocks in the underlying market, and that the options implied price volatility is provoked by the information shock occurring in both the cash and options markets to a higher degree. Through the volatility impulse response function, this study shows that the options implied prices are more sensitive to innovations in both markets.
De-Hai Li,Tian-Jiao Zhu,Hong-Bing Liu,Yu-Chun Fang,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.8
Four known butenolides were isolated from the ethyl acetate extracts of the culture broth of the marine-derived bacterium, Streptoverticillium luteoverticillatum, by bioassay-guided fractionation. The structures were identified on the basis of spectral data. The absolute configuration of compound (1) was determined by CD spectrum for the first time. Compounds 1-4 showed in vitro cytotoxicity against the murine lymphoma P388 and human leukemia K562 cell lines. This is the first report on the isolation of butenolides from the marine bacterium, Streptoverticillium luteoverticillatum, and their cytotoxic activities.
DE-GUO WANG,SI-WEI ZHANG,REN-YANG HE,MING-YUAN LI 한국트라이볼로지학회 2002 한국트라이볼로지학회 학술대회 Vol.2002 No.10
The mechanisms of formation of transfer film under the condition of wear of Steel AISI1020 by natural rubber were investigated. The transfer film was observed and the formation mechanisms were clarified. The formation process of transfer film on the worn surface of the steel could be divided into two stages. Firstly, the adhesive layer emerged on the worn surface of the steel by adhesion of natural rubber, in which the macromolecular chains of natural rubber joined to the surface of the steel by Van der Waals' force. And then, the iron atom and metal oxide reacted with the macroradicals of natural rubber in the adhesive layer and produced Fe-polymer compound. As a result, the transfer film was formed on the worn surface of the steel. The transfer film was joined to the worn surface of the steel by the chemical bonds and electrostatic force.
Lu, De-Yi,Mao, Xu-Hua,Zhou, Ying-Hui,Yan, Xiao-Long,Wang, Wei-Ping,Zheng, Ya-Biao,Xiao, Juan-Juan,Zhang, Ping,Wang, Jian-Guo,Ashwani, Neetika,Ding, Wei-Liang,Jiang, Hua,Shang, Yan,Wang, Ming-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13
Nogo protein, encoded by gene reticulon-4 (RTN4), includes three major isoforms by different splicing, named Nogo-A Nogo-B and Nogo-C. Nogo proteins play an important role in the apoptosis of cells, especially in tumor cells. RTN4 single nucleotide polymorphisms (SNPs) can influence the efficiency of transcription and translation thus being related with an individual's predisposition to cancer. The CAA insertion/deletion polymorphism (rs34917480) within RTN4 3'-UTR has been reported to be associated with many cancer types. In order to investigate the relationship between this polymorphism and susceptibility to non-small cell lung cancer (NSCLC) in the Chinese population, we conducted the present case-control study including 411 NSCLC patients and 471 unrelated healthy controls. The genotype distributions were significantly different between cases and controls (p=0.014). We found that the del allele could significantly increase NSCLC risk (ins/ins vs ins/del: p=0.007, OR 1.46, 95%CI=1.11-1.93; dominant model: p=0.004, OR 1.47, 95%CI=1.13-1.92 and allele model: p=0.008, OR 1.35, 95%CI=1.08-1.67). This association was stronger in participants over 60 years old, males and smokers. We therefore conclude that the CAA insertion/deletion polymorphism (rs34917480) contributes to non-small cell lung cancer risk in Chinese population. Age, sex and environmental exposure are also related to carcinogenic effects of rs34917480.