Development of Simultaneous Analytical Method for ImidazolinoneHerbicides from Livestock using LC-MSMS

Hyo-Min Heo,Hyeong-WookJo,Kyu-Won Hwang,Jung-Hun Sun,Joon-Kwan Moon 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11

Simultaneous analytical method using LC/MSMS for imidazolinone herbicides from livestock (egg, milk, beef, pork and chicken) for monitoring was developed with QuEChERS preparation. Sample weighing (5g) in a 50mL conical tube, added 0.1 M potassium phosphate dibasic solution (5mL) was shaken for 10min. After 0.5mL 6 NHCl, 10mL acetonitrile were added, it was shaken for 10min. And QuEChERS extraction salt (Original method, 4g MgSO₄, 1g NaCl) were added to the sample in the 50 mL conical tube. The mixture was strongly shaken for 1 min and was centrifuged at 3,000g for 10 min. The acetonitrile layer was purification with dSPE (150mg MgSO₄, 25mg C18) and was centrifuged at 13,000g for 5min. The supernatant was filtered with a membrane filters (pore size: 0.2um) before analysis. ME (%, Matrix effect) for almost analytes range were -6.56 to 5.70%. MLOD (Method LOD) and MLOQ (Method LOQ) was calculated by S/N ratio. MLOQs were 0.01 mg/kg. The linear correlation coefficients (r2) were > 0.99 within the range of 2 ~ 100 ug/kg for all of the 3 imidazolinone herbicides. The percentages (of imidazolinone herbicides) recovers were in the range of 84.1 ~ 110.6% (0.01mg/kg level), 90.8 ~ 97.1% (0.1mg/kg level) and 94.4 ~ 102.1% (0.5mg/kg level) within the validation criteria (recover; 70-120% with RSD < 20%)

현대시조의 종장에 나타난 특성 연구

민병관(Min, Byeong-Kwan) 한국시조학회 2014 시조학논총 Vol.40 No.-

이 연구는 시조학계에서 지속적으로 논란이 되고 있는 정형성 문제와 정체성 문제를 해결하기 위한 기초 작업이다. 현대시조의 정형성을 규명하기 위한 연구의 일환으로 현대시조의 종장에 나타난 특성을 분석하였다. 현대시조 중 대표적인 작품을 모아 엮은 현대시조 100인 선집에 실린 101편의 시조(종장 330개)를 대상으로 분석한 결과 다음과 같은 결론을 얻었다. 첫째, 현대시조 종장의 배열 형태는 고시조에 비해 다양한 양상을 보여주고 있다. 1연에서 5연, 1행부터 6행 등 다양한 방식으로 배열하고 있다. 또한 초장, 중장과 독립된 연으로 배열한 경우가 30% 가까이 된다. 이것은 초장과 중장에 비해 종장의 의미를 더 부각시키고자 한 의도의 산물이다. 둘째, 종장의 제1음보는 모두 3음절로 이루어졌으나, 2~4음보는 1음절부터 10음절까지 다양하게 이루어져 있다. 고시조에 비해 현대시조에서는 음절 과잉 현상이 많이 일어나고 있다. 이러한 현상은 현대사회의 다양화와 다변화, 현대인의 복잡한 정서 등을 작품에 반영한 결과이다. 셋째, 종장의 종결어는 서술형과 명사형이 65% 이상이고, 연결형도 15.5% 가량 된다. 고시조의 종결사는 의문형이나 감탄형이 대부분인데 반해, 현대시조는 5.7%에 불과하다. 이것은 고시조가 청자 지향적이고 관계성을 중시한 문학이라면, 현대시조는 화자 중심적이고 개인성을 강조한 결과일 것이다. 넷째, 종장에 쓰인 문장부호의 숫자는 0개~8개까지 다양한 양상을 보여주고 있다. 고시조에 비해 문장부호를 많이 사용한 것은 문장부호가 의미를 강화하는 효과가 있다는 인식에서 비롯된 것이다. 문장부호를 통해 표현하고자 하는 의미를 보충하고 시각적 효과, 여운의 효과 등을 드러내고자 한 의도의 산물이다. 다섯째, 종장의 의미구조를 분석한 결과, ‘00/00, 0/0/00, 0/0/0/0, 00/0/0’ 형태와 같이 구(句)에 대한 인식이 분명한 것이 95% 가량이었다. 반면에 ‘0/00/0, 000/0, 0/000’ 형태와 같이 구에 대한 인식이 불분명한 것이 5% 가량이었다. 이것은 대다수 현대 시조시인들이 시조의 각 장이 2구로 구성된다는 정형성에 대한 인식을 창작에 반영한 결과이다. 다만 일부 종장에서는 시조의 정형성에서 벗어나 실험정신에 입각한 새로운 시도를 하고 있다. 이상과 같이 현대시조의 종장을 여러 측면에서 분석한 결과 그 특징은 크게 두 가지로 요약할 수 있다. 첫째, 현대시조의 종장은 고시조의 정형성을 인식하고 그것을 유지하려는 노력의 결과물이다. 둘째, 현대시조의 종장은 고시조와 변별되는 형식상의 새롭고 다양한 시도를 보여주고 있다. 결론적으로 현대시조의 종장은 고시조의 정형성을 잃지 않으면서도, 동시에 창조적으로 계승하고자 하는 노력의 산물이다. The purpose of this study was to investigate characteristics that the last line of the modern sijo has as a basic step to determine the very identity of that sijo. For this purpose, this researcher selected and analyzed representative 101 pieces of the modern sijo. The findings of this study can be summarized as follows. First, the modern sijo has more diverse arrangements of the last line than the ancient sijo has. For example, that line is arranged in 1 to 5 stanzas, 1 to 6 rows or in many other ways. By the way, nearly 30% of the analyzed verses have the last line separately arranged from the first and the second lines. What is implied here is poets’ intent to make the last line more meaningful than the two others. Second, the first foot of the last line usually consists of 3 syllables, but the 2nd to 4th foot, 1 to 10 syllables. In other words, the overuse of syllables are found in the modern sijo in comparison to the ancient sijo. This may be because the modern Korean verse reflects various, diverse aspects of the modern society and emotional complexities of modern people. Third, 65% of the surveyed pieces have the ending words of the last line that are predicative or noun and 15.5%, connective. Among the pieces of the ancient sijo, more than 50% are interrogative or exclamatory in the ending words of the last line. These types are found in only 5% of the modern sijo. This may be attributed to the fact that the ancient sijo is listener-centered and relation-oriented, while the modern sijo, speaker-centered and personality-oriented. Fourth, the modern sijo has many punctuation marks, or 0 to 8 in the last line. The reason why those marks are more found in the modern sijo than in the ancient one is because there’s a recognition that punctuation marks have the effect of strengthening meanings. In other words, underlying the frequent use of punctuation marks is an intent by writers of the modern sijo to supplement meanings they wished delivering and present some visual and suggestive effects. Fifth, among the pieces of the modern sijo surveyed here, 95% are ‘00/00’ in semantic structure and 5%,‘0/000’. This suggests a notion that sijo is a fixed form of verse each of whose lines consists of 2 phrases was reflected in the creation of those pieces. On the other hand, however, it may be seen a new attempt that is experimental. When all these findings are considered, it is possible to reach a conclusion that the last line of the modern sijo largely have the following two charac teristics. First, the last line of the modern sijo is an outcome of efforts to recognize and maintain the formal fixity of the ancient sijo. Second, that last line shows new attempts in style to be discriminated from the ancient sijo. Thus, the last line of the modern sijo is the outcome of struggles to keep formal characteristics of the ancient sijo on one hand and creatively succeed them on the other hand.

Identification of sperm mRNA biomarkers associated with sex-determinant reagent treatment in semen of Korean Native Cattle

Munkhzaya Byambaragchaa,Kwan-Sik Min,Hyun-Kim,Myung-Hum Park 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

This study was conducted to analyze the specific genes associated with sex-determination in semen of Korean Native cow. Male fertility is dependent upon the successful perpetuation of spermatogenesis that is a highly organized process of germ cell differentiation occurring within the seminiferous tubules in the testis. The highly organized spermatogenesis requires accurate, spatial and temporal regulation of gene expression governed by transcriptional, post-transcriptional and epigenetic processes. Recently, the farmers have been interesting in the male or female of calves in the their farm. In first, we analyzed the semen supplied from Hanwoo Improvement Center, NongHyup. The sperm motility in Hanwoo was decreased approximately 10 % in the 30 min after sex-determinant reagent. However, Holstein' sperm motility was decreased to 60-70% after 15 min and the motility was considerably decreased to 20-30 % after 30 min. Next, we analyzed the sperm specific expression genes both male- and female reagents treated-group. The real-time PCR results suggest that the selected genes (GIMP4, TMEFF1, RAC2, ABI2, RAC1, and CLUS) were highly expressed in the group treated with the male reagent compared to female reagent treated group and untreated-group. In the present study, although X or Y gene is play a key role in the sex-determination of mammalian, we suggest that the selected genes may be involved in the sex-determination.

비린내와 조직감이 개선된 치즈 토핑 반건조 조미 피조개(Scapharca broughtonii)의 개발 및 특성

강상인 ( Sang In Kang ),김예진 ( Ye Jin Kim ),이지운 ( Ji Un Lee ),박지훈 ( Ji Hoon Park ),최관수 ( Kwan Su Choi ),황지영 ( Ji-young Hwang ),허민수 ( Min Soo Heu ),이정석 ( Jung Suck Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6

Methods for the development of home meal replacement seafood tailored to consumer needs for the advanced use of Broughton’s ribbed ark Scapharca broughtonii (BRA) in Korea are required. In this study, we developed a cheesetopped, semi-dried, and seasoned Broughton’s ribbed ark (S-BRA) tailored for the younger generation with an improved texture and fish odor. The optimization of conditions to improve the texture and fish odor was performed using RSM. The design of the model was appropriate because there was no significant difference (P>0.05) between the predicted and actual values of moisture content, hardness, and overall acceptance, and the optimal preparation conditions were a vinegar content of 2.68%, a soaking time of 62 min, a drying temperature of 60℃, and a time of 162 min. The SBRA manufactured under these optimal conditions exhibited a lower odor intensity compared to the unsoaked and undried control, suggesting that the fish odor of S-BRA has been improved. The moisture content related to the texture of the S-BRA was lower than that of the control, and the hardness was higher. Therefore, the S-BRA developed in this study will appeal to people of all ages, especially the younger generation; their consumption is expected to increase.

폐렴구군 Type 23 협막다당류 생산의 최적화

민관기,표석능,이동권 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-

Abstract-Streptococcus pneumoniae (pneumococcus) is the most frequent causative agent of acute bacterial pneumonia. Outstanding characteristic of pneumococcus is an ample polysaccharide capsule that is highly antigenic agent and is the major factor for classification of pneumococcus into more than 94 serotypes. In this study, production of capsular polysaccharide from Streptococcus pneumoniae type 23 was optimized by supplementation of metal ions or by modulation of culture conditions. When brain heart infusion (BHI) broth was supplemented with 1 mM concentration of CaCl_2, CuSO_4 and MgSO_4, growth of pneumococcus as well as polysaccharide yield were stimulated. Also slight aeration gave rise to better polysaccharide yield.

Priming 처리가 담배 pellet 종자의 발아에 미치는 영향

민태기,유관식 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5

Seed priming and pelleting of tobacco seeds (KF109) were conducted to get informations on the priming effect of intact seeds and pelleted tobacco seeds. Seeds were primed with -0.8MPa polyethylene glycol (PEG) 8000 solution and matriconditioned with water and moist with 60% of 0.3M KNO₃ in perlite. Intact seeds primed in either osmoticum germinated more rapidly and higher in final germination percentage than untreated seeds at 25℃ and were eliminated light requirement for germination. The effect of elimination of light requirement for the intact seeds was the greatest in 0.3M KNO₃ treatment for 5 days. Pelleted seeds, in all treatments, were more reduced in germination rate than intact seeds in either primed or non-primed. In pelleted seeds, the germination rate was a little higher in the seeds primed with 0.3M KNO₃ than untreated or primed with PEG.

표준기상데이터의 개발에 관한 연구

趙敏寬 강남대학교 1998 (강남대학교)논문집 Vol.32 No.4

간호 지식관리시스템 개발

민효숙,문원희,김미자,김선미,황윤신,황관옥,심희숙 충남대학교 간호과학연구소 2006 충남대 간호학술지 Vol.9 No.1

The purpose of this study were to develop Knowledge Management System for the nurse and to identify its effectiveness. The program was developed in development of the contents and programing. And the system was operated to 85 nurse for three weeks. Data collection were performed during the period of May 1, 2005 to December 30, 2005. The data was collected from 85 nurses using questionnaires. In order to ascertain the validity of clinical application of the system, Prior experience of the computer mediated communication, user satisfaction and utilization were analyzed. The instruments for the study were satisfaction(Kim, 2000), utilization(Kim, 2002). The collected data were analysed by descriptive statistics, using SPSS WIN 11.0 program. The results showed average score which is the satisfaction(6.09) showed middle satisfaction scores. It is concluded that if Nursing Knowledge Management System is operated considering all the above factors, It wound be one of the best qualified continuing clinical method for nurses.

대학생의 학업성취 수준 실태 분석 연구

김형관,신현석,서민원,황기우 고려대학교 교육문제연구소 2001 敎育問題硏究 Vol.15 No.-

본 연구는 대학생의 학업성취 수준을 사전에 선정된 사회 문화적 배경요인에 따라 실증적으로 분석하여 그 실태를 알아보는데 목적이 있다. 본 연구는 연구주제와 관련된 선행 문헌고찰을 바탕으로 대학생의 학업성취를 인지적 측면에서 ① 실제 학점 평균과 ② 지각된 성취수준(전공지식 습득, 교양지식 습득, 논리·분석적 사고력, 창의력) 등 두 가지로 개념화하였다. 그리고 학업성취와 관련된 사회 문화적 배경요인은 크게 대학생의 ① 개인적 배경요인, ② 가정배경요인, ③ 대학배경요인 등 세 가지로 구별하였다. 본 연구의 대상은 전국에서 표집된 6개 종합대학 1,781명의 대학생들이다. 이들을 대상으로 학업성취요인과 사회 문화적 배경요인들을 설문화하여 수집된 자료를 분석한 결과, 개인적 배경변인에서는 계열별, 거주형태, 일일 평균 공부시간 등의 변인은 학업성취에 차이를 가져오는 것으로 나타났다. 가정 배경변인에서는 출신지역과 부모의 학력 정도에 따라 학업성취 수준이 다르게 나타났다. 한편, 대학 배경변인 별로는 대학설립 유형, 학교 소재지, 입학전형 방식 등의 변인들이 학업성취 수준의 차이를 가져오는 것으로 밝혀졌다.

최대근력부하와 폭발적 근력부하에 의한 신경-근피로와 그 회복과정 비교 분석

유관호,이무연,이승훈,김민섭,육조영,임정일,임완기,김대경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The result which was gained from this study is as follows. From the conclusion of this study it was confirmed that resistant motion at high level strength brings about rise in density of lactate in blood at the same time decline of muscular power and generates acute fatigue of nerve-muscle accompanied by decline in electrical activity of muscle. On the other hand explosive loading produced fatigue at body center and decline in nervine-muscular activity particularly for men, In case of women explosive load did not produce fatigue in the same level as men and they were also quicker in recovery after exercise. Based on 2 types of loading method used in this study there was clearly difference in the process of restoration from resistant motion. For greater decline in muscular power and electromyogram activity more longer period of recovery was require.