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김남희,구재명,이민석,Kim, Nam Hee,Koo, Jae Myung,Rhee, Min Suk Korean Society of Food Science and Technology 2015 한국식품과학회지 Vol.47 No.5
This study determined the thermal resistance of Bacillus cereus, Escherichia coli O157:H7, and Listeria monocytogenes in multi-grain soymilk and proposes processing conditions that meet the national standard for retort food products in Korea. D and z values were calculated from thermal inactivation kinetic curves after heating at 55, 60, and $65^{\circ}C$. The D value for B. cereus at $55^{\circ}C$ was the highest (22.8 min), followed by that for E. coli O157:H7 (18.8 min) and L. monocytogenes (17.6 min). At $60-65^{\circ}C$, the order was L. monocytogenes ($D_{60-65^{\circ}C}=3.4-0.9min$), E. coli O157:H7 (3.0-0.3 min), and B. cereus (1.2-0.3 min). The z values for these species were 5.2, 5.5, and $7.7^{\circ}C$, respectively. The Korean national standard for retort food products was achieved by thermal processing at $124{\pm}2^{\circ}C$ for 0.3-2.2 min. This study provides useful data for ensuring both the microbiological safety and product quality of multi-grain soymilk products.
흡연이 Gastric Emptying Time에 미치는 영향
조민구(Min Koo Cho),정순영(Sun Young chung),김소연(So Yon Kim),문희승(Mun Hee Seung),김진석(Jin Suk Kim),이석호(Suk Ho Lee),이권전(Gwon Jun Lee) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2
The effect of cigarette smoking on gastric emptying was examined in 25 healthy volunteers by means of Tc-sulfur colloid labeled-solid meal. The volunteers underwent GET measurement two times: before smoking and after smoking two cigarettes. Before smoking, the each average of GET T25% T50%, T75%, was 18 +- 5.2, 40 +- 6.7, 90 +- 18.4 min and after cigarette smoking the each average of GET T25%, T50%, 75% was 30 +- 8.3, 64 +- 12.6, 114 +- 7.0 min. We concluded that cigarette smoking significantly delayed gastric emptying of a solid meal (p< 0.05).
Eucheuma spinosum으로부터 다양한 효모를 이용한 바이오에탄올 생산
김민지(Min-Ji Kim),김정수(Jung-Soo Kim),라채훈(Chae Hun Ra),김성구(Sung-Koo Kim) 한국생물공학회 2013 KSBB Journal Vol.28 No.5
Ethanol fermentations were performed using separate hydrolysis and fermentation (SHF) processes with monosaccharides from pretreated seaweed, Eucheuma spinosum as the biomass. The pretreatment was carried out with 11% (w/v) seaweed slurry and 150 mM H₂SO₄ at 121℃ for 40 min. Enzyme hydrolysis after H₂SO₄ pretreatment was performed with Celluclast 1.5 L at 45℃ for 24 h. Five % active charcoal were added to hydrolysate to removed 5-hydroxy methylfurfural. Ethanol fermentation with 11% (w/v) seaweed hydrolysate was performed for 72~96 h using Kluyvermyces marxianus, Pichia stipits, Saccharomyces cervisiae and Candida tropicalis. Ethanol concentration was reached to 18 g/L by K. marxianus, 16 g/L by P. stipitis, 15 g/L by S. cerevisiae and 10 g/L by C. tropicalis, respectively. The ethanol yield from total monosugar was obtained 0.50 and ethanol productivity was obtained 0.38 g/L/h by K. marxianus.
Saccharina japonica를 이용한 전처리 및 분리당화발효와 동시당화발효로부터 에탄올 생산
김민지(Min-Ji Kim),김성구(Sung-Koo Kim) 한국생물공학회 2012 KSBB Journal Vol.27 No.2
Ethanol fermentations were carried out using simultaneous saccharification and fermentation (SSF) and separated hydrolysis and fermentation (SHF) processes with monosaccharides from seaweed, Saccharina japonica (sea tangle, Dasima) as the biomass. The pretreatment was carried out by thermal acid hydrolysis with H₂SO₄ or HCl. Optimal pretreatment condition was determined at 10% (w/v) seaweed slurry with 37.5 mM H₂SO₄ at 121℃ for 60 min. To increase the yield of saccharfication, isolated marine bacteria Bacillus sp. JS-1 was used and 48 g/L of reducing sugar were produced. Ethanol fermentation was performed using SSF and SHF process with Pachysolen tannophilus KCTC 7937. The ethanol concentration was 6.5 g/L by SSF and 6.0 g/L by SHF.
H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향
정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4
Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.
강민구(Min Koo Kang),신관우(Gwan Woo Shin),이상일(Sang Ill Lee) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.11
본 연구의 목적은 칼슘염을 함유한 레미콘 슬러지를 이용하여 불소함유폐수를 물에 난용성인 CaF₂ 화합물 형태로 침전시켜 처리하는데 있어 pH, 레미콘슬러지 주입량, Seed물질 주입량, 교반속도, 교반시간의 최적 조건을 도출하고자 하는데 있다. 실험결과 레미콘 슬러지를 이용한 대상폐수의 CaF₂ 침전 반응에서 함수율 및 불소의 제거효율 등을 고려할 경우 최적의 pH 6, 레미콘 슬러지 주입량은 10 g/L, Seed 물질 주입량은 2 g/L, 교반속도은 100 rpm, 교반시간은 60 min으로 관찰되었다. 이 때 Seed 물질의 주입은 불소제거효율에는 영향이 없는 것으로 나타났지만 침전물의 형성이 되는 반응을 촉진시키고 플럭형성을 원활하게 하여 고액 분리가 잘 되어 함수율이 낮아지는 결과가 나타났다. The purpose of this study was performed to investigate the optimum conditions of pH, concrete sludge, seed dosage, mixing intensity, operation time in treating fluoride-containing wastewater as CaF₂ using the ready-mixed concrete sludge. Considering fluoride removal, water content, that pH 6, concrete sludge dosage of 10 g/L, Seed dosage (CaF₂) of 2 g/L, mixing intensity of 100 rpm and operation time of 60 min were found to be optimum. Correspondingly, removal of fluoride and water content was about 85% and 64%, respectively. Increase in amount of seed dosage did not affect fluoride removal efficiency. but the result that the water content is decreased was shown up in occuring the solid-liquid separation well.
Byeong Cheol Min,Bon Wook Koo,Ki Seob Gwak,Hwan Myeong Yeo,Joon Weon Choi,In Gyu Choi 한국목재공학회 2011 목재공학 Vol.39 No.2
In this study, dilute acid pretreatment of Liriodendron tulipifera was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (gig) and enzyme adsorption was 44% (gig). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When L. tulipifera was pretreated with 1% sulfuric acid at 160℃ for 5 min., hemi-cellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at 160℃ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.