천연 갈변 억제제 개발을 위한 양파,사과 및 감귤 과피 추출물의 항산화 및 갈변 저해 효과

장민선 ( Min Sun Chang ),안세진 ( Se Jin An ),정문철 ( Moon Cheol Jeong ),김동만 ( Dong Man Kim ),김건희 ( Gun Hee Kim ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3

This study was carried out to investigate antioxidative properties of various extracts and antibrowning effects of extracts in apple slices were investigated by ΔE value and PPO relative activity. Apples were cut into 1.5 ㎝ thickness and they were dipped in 1% extract solutions(OW: water extracts of onion, OE: 80% EtOH extracts of onion, AW: water extracts of apple, AE: 80% EtOH extracts of apple, MW: water extracts of mandarin orange peel, ME: 80% EtOH extracts of mandarin orange peel) for 1 min. OW showed higher than the other treatments for total phenolic contents(94.35 ㎎/g), PPO inhibition(74.00%). And the highest DPPH free radical scavenging activity(40.27%) measured in ME. ΔE value of apple slices dipped in MW was 2.37 whereas ΔE value of apple slices dipped in AW was 12.12. These results suggest that onion and mandarin orange peel extracts should be a potential source for controlling browning during storage of apple slices.

Au 전극의 DDT 보호막 처리 및 그 전기적 효과와 응용

최민수(Min-Su Choi),이동진(Dong-Jin Lee),김태근(Tae-Gun Kim),황성우(Sung-Woo Hwang) 대한전기학회 2010 전기학회논문지 Vol.59 No.1

We report the passivation characteristics of dodecanethiol (DDT) molecules on gold electrodes of field effect transistor bio-sensors. The leakage current between the electrolyte and the electrode can be as small as 3pA when 7 mM of DDT passivation is performed. The DDT layer is also shown to resist the attack of acid up to 120 mins.

이차함수에서 두 변량사이의 관계 인식 및 표현의 발달 과정 분석: 민선의 경우를 중심으로

이동근 ( Dong Gun Lee ),문민정 ( Min Joung Moon ),신재홍 ( Jaehong Shin ) 한국수학교육학회 2015 수학교육 Vol.54 No.4

The aim of this qualitative case study is twofold: 1) to analyze how an eleventh-grader, Min-Seon, conceive and represent a pattern of change between two varying quantities in a quadratic functional situation, and 2) further to help her form a concept of ``derivative`` as a tool to express the relationship with employing a concept of ``rate of change.`` The result indicates that Min-Seon was able to construct graphs of piecewise functions that take average rates of change as range of the functions, and managed to conjecture the derivative of a quadratic function, y=x ^{2}. In conclusion, we argue that covariational approach could not only facilitate students`` construction of an initial function concept, but also support their understanding of the concept of ``derivative.``

Bioconversion of ginsenoside Rc into Rd by a novel α-L-arabinofuranosidase, Abf22-3 from Leuconostoc sp. 22-3: cloning, expression, and enzyme characterization.

Liu, Qing-Mei,Jung, Hae-Min,Cui, Chang-Hao,Sung, Bong-Hyun,Kim, Jin-Kwang,Kim, Song-Gun,Lee, Sung-Taik,Kim, Sun-Chang,Im, Wan-Taek N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.4

<P>A novel α-L-arabinofuranosidase (Abf22-3) that could biotransform ginsenoside Rc into Rd was obtained from the ginsenoside converting Leuconostoc sp. strain 22-3, isolated from the Korean fermented food kimchi. The gene, termed abf22-3, consisting of 1,527 bp and encoding a protein with a predicted molecular mass of 58,486 Da was cloned into the pMAL-c2x (TEV) vector. A BLAST search using the Abf22-3's amino acid sequence revealed significant homology to that of family 51 glycoside hydrolases. The over-expressed recombinant Abf22-3 in Escherichia coli BL21 (DE3) catalyzed the hydrolysis of the arabinofuranoside moiety attached to the C-20 position of ginsenoside Rc under optimal conditions of pH 6.0 and 30 C. This result indicated that Abf22-3 selectively converts ginsenoside Rc into Rd, but did not catalyze the hydrolysis of glucopyranosyl groups from Rc or other ginsenosides such as Rb1 and Rb2. Over-expressed recombinant enzymes were purified by two steps with amylose-affinity and DEAE-cellulose chromatography and then characterized. The kinetic parameters for α-L-arabinofuranosidase showed apparent Km and Vmax values of 0.95 0.02 μM and 1.2 0.1 μmol min(-1) mg of protein(-1) against p-nitrophenyl-α-L-arabinofuranoside, respectively. Using a purified MBP-Abf22-3 (10 μg/ml), 0.1 % of ginsenoside Rc was completely converted to ginsenoside Rd within 20 min.</P>

질트리코모나스(Trichomonas vaginalis)의 효소활성에 관한 전자현미경적 관찰

민득영,류재숙,박건채 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Trichomonas vaginales is one of the parasitic flagellates found in the vaginal cavity of female and the urogenital tract of male. The presnet study was aimde to evaluate the effect of antiserum from immunized Wkstar rats on activities of acid phoshpatase (AcPase) and adenosine triphosphatase (ATPase) of T. vaginalis. Trophozoites were subcultured in Diamond's TPS-1 medium at 37℃ in CO₂ incubator. T. vaginalis were incubated in mixed medium containing 26%(v/v) of antiserum for minutes, then treated with substrates for enzyme activityes. Parasites were embedded in Epon and sectioned for transmission electron microscopy. The results observed are as follows: 1. AcPase activities. In normal prarsites strong activities were observed on Golgi complex and a few of wacuoles. Weak reactions also observed in nuclear matrix, kinetosome and hydrogenosome. In cases treated with antiserum plasma membrane were parially destroyed. Vacuoles were increased in number and revealed strong reactivites. 2. ATPase activities. In normal parasites ATPase were observed dominantly in nucleus. In parasites treated with antiserum activities in nucleus were stronger than those of normal ones, and weak activities were seen along plasma membrane. In conclusion it is assumed that antiserum does increase AcPase activity, but reduces ATPase activity in T. vaginalis.

불완전휴식이 에너지대사와 혈중지질에 미치는 영향

민범일,허건홍 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2

This study was designed to inverstigate the effects of resting methods - active rest and inactive rest-after exercise of maximum of gradual load, on energy metabolism and Blood Lipids. I surveyed 6 male basketball players in a high school in order to investigate the effects on exercise spot and analysed the relations of blood variations before and after exercise of maximum of gradual load with a treadmill and 3, 6, l0minutes after recovery step. the results was as follows; In the difference between active and inactive resting method, to make a comparatived of Albumin, Glucose and Total-Protein appeared respectively 6minutes after the recovery step. Albumin and Total-Protein were significant differences (P<.05) in group and time, glucose was significant differences(P<.05) in time and method x time. In the difference between active and inactive resting method, to make a comparatived of Total-Cholesterol and Triglyceride appeared respectively each group. Total-Cholesterol was significant differences(P<.05) in group and time, Triglyceride was significant differences(P<.05) in method.

中風의 治法 중 潛陽法에 대한 문헌고찰

閔健祐,朴鍾赫,鄭智天 동국대학교 한의학연구소 2000 東國韓醫學硏究所論文集 Vol.9 No.-

潛陽法은 平肝治法의 일종으로 刊陽이 上亢해서 나타나는 頭目昏暈, 수족추휵, 구안괘사, 舌乾語澁, 頭痛不止, 猝然倒地 등의 症狀에 쓰는 治法이며 中風의 標實證을 다스리는 목적으로 多用되고 있다. 中風에서의 活用은 淸代에 이르러 본격화되었는데, 葉天士는 "자액식풍, 濡養營絡, 補陰潛陽"을 사용하였고, 張伯龍은 "潛鎭攝納" 하는 치료법을 사용하였다. 中風의 病理가 本虛標實하므로 항상 滋陰, 補水하는 治法과 相互補完的으로 사용되고 있다. 潛陽法이 多用된 병증은 刊胃陰虛, 風陽上擾로 인한 中經絡證과 中腑證 중에서 陽閉證이며, 脫證의 일부와 中風日久로 因한 癲癎에도 사용 되었다. 潛陽藥 중 珍珠母, 石決明, 玳瑁, 牡蠣, 見齒, 龜板, 鱉甲 등 介類의 효력이 뛰어나고, 治方으로는 鎭肝??風陽, 虎潛丸, 固本丹, 復脈湯, 天麻鉤藤飮, 羚羊角湯 등이 활용되고 있다. The methods of checking the exuberance of yang (潛陽法) are a kind of medical treatments Lvhich cure various symtoms caused by hyperactivity of liver-yang, at the same time used for treating apoplexy. Thus, I investigated the application of checking the exuberance of yang to treat stroke. The conclusions are as follows. We can find application of checking the exuberance of yang to apoplexy on Synopsis of the Gold Chamber. After Ye Gui who persisted the theory of endogenous wind, many physicians applied the method of checking the exuberance of yang and this method has usually applied with nourishing yin. The methods of checking the exuberance of yang are concretely applied as follows. 1) a portion of apoplexy involving the channels and collaterals, which is caused by deficiency of \-in and wind-fire pathogen. 2) excessive-syndrome of coma accompanied by heat syndrome. 3) epilepsy caused old apoplexy. The majority of shells for example abalone shell, hawksbill shell, oyster shell, tortoise-shell etc. are the most frequent use of herbal drug which check the exuberance of yang. The precriptions of checking the exuberance of yang are Ho-jam-hwan(虎潛丸), Ko-bon-dan(固本丹), Cheon-ma-gu-deung-eum(天麻鉤藤飮), Chin-gan-sik-pung-tang(鎭肝湜風湯) etc.

충남 서북부지역 여성의 질세포진 검사에 대한 임상적 고찰

이석민,선우재근,장경택,서수형,김민관,배동한 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

Pap smear were performed to 30,294 women living in the north-west of Chung-nam. The result are summarized below 1. Positive vaginal cytology was 1.09 percent. 2. 72 percents women in their 30's and 40's experienced vaginal cytology. 3. Vaginal cytology was performed at a decreasing rate as age increases. 4. Positive cytology rate is 2.42% higher than average for elderly women over the age of 65. The present date indicates that pap smear is being widely recognized to be important but they needs to be continued advocacy for pap smear.

2단 평행구조 로보트 프로그램 개발

박근우,이민기 國立 昌原大學校 産業技術硏究所 1996 産技硏論文集 Vol.10 No.-

This paper introduces the development of program for the double parallel robot which is applied to a machining work. The robot system consists of a robot, PC (personal computer), DMC (DSP Motion Controller), and machining tools. We analyze the motions of robot by an inverse kinematics +and drive the motions by DC Servo motors. In order to actuate the motor, we use the motion functions of the DMC, which is implemented in "C" language. Without adding a teaching box, we teach the robot with keyboard itself. Furthermore, we develope a 3-D graphics simulator to investigate the feasibility for application program in off-line.

마이크로홀 드릴링 머신의 개발 및 절삭성능 평가

김민건,유병호 한국공작기계학회 2001 한국생산제조학회지 Vol.10 No.5

In this paper, drill feed mechanism, cutting depth measuring device and sensing buzzer of drill contact were investigated in order to develop the micro-hole drilling machine. Also, measuring device of cutting resistance was developed in order to estimate cutting resistance from change of cutting condition. The results show that extremely-low feed rate(less then 17㎛/s) can be done and cutting depth can be measured by up to 1㎛ with develope drilling machine. Accordingly we could assemble a very cheap micro-hole drilling machine(ø0.05∼0.5mm). Also we got the some properties of cutting performance i.e. under the same condition, cutting torque decreases as increase of spindle speed and rapid feed of drill brings about the inferior cutting state under low spindle speed.