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The Isolation and Identification of Soybean Pod and Stem Blight in Taiwan
Min-Jung Seo,Chien-Hua Chen,Kil Hyun Kim,Seuk-Ki Lee,Hong-Tae Yun,Yeong-Hoon Lee,Beom-Young Son,,Jung-Tae Kim,Jin-Seok Lee,Hwan-Hee Bae,Chang Hwan Park,Seong-Bum Baek,Jeom-Ho Lee 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.3
미이라병은 Diaporthe/Phomopsis complex에 의해 유발되는병으로 콩 재배기간 중 따뜻하고 습한 환경에서 종자가 성숙되면 감염률이 높아지며 감염된 콩 종자는 외관상 품질뿐만아니라 종자 활력이 저하된다. 미이라병에 대한 연구를 수행하기 위하여 대만에 위치한 아시아채소개발연구센터(AVRDC)의 콩 시험포장에서 미이라병 병징을 보이는 콩 줄기를 채집하고 이로부터 3개의 곰팡이 균주(isolate)를 분리하였다. 배지위에서의 곰팡이 균사의 생육특성, 현미경하에서 관찰된 알파,베타 분생자(conidia)의 모양 그리고 PCR-RFLP 분석으로, 3개의 균주는 Diaporthe phaseolorum var. sojae 으로 확인되었다. 한편, 미이라병 저항성 육종을 위해서는 유전자원과 계통의 검정이 선행되어야 하는데, 인공접종을 위해서 분생자의최적 배양조건을 탐색하였다. 그 결과 배지는 PDA, 온도는24oC에서 잘 배양되었으며, 일장은 암조건에서는 균사체만 유도되고 분생자는 유도되지 않았으며, 24시간과 15시간의 일장에서는 균사체 유도 및 분생자의 유도 정도에 차이가 없었다.또한 잎-줄기와 꼬투리, 두 개의 접종 부위에 따른 미이라병감염률을 조사하였는데, 두 접종 부위에 따른 미이라병 감염정도는 통계적인 유의차는 나타나지 않았으나 잎-줄기에 접종한 개체 보다 꼬투리에 접종한 개체의 종자 감염률이 높은 경향을 보였다. Phomopsis seed decay (PSD) caused by Diaporthe phaseolorum var. sojae (Lehman) Wehmeyer, Diaporthe phaseolorum (Cooke & Ellis) Sacc. var. caulivora Athow & Caldwell, and Phomopsis longicolla Hobbs reduces quality of soybean (Glycine max (L.) Merr.) seed when it is wet and warm condition during seed maturation period. To study of the PSD in Taiwan in March 2008, three unidentified fungal isolates (isolate1, isolate2 and isolate3) were isolated from soybean stems infected with pod and stem blight which is associated with seed decay. Based on their morphological and genotypic characteristics, three isolates were regarded as Diaporthe phaseolorum var. sojae. For PSD assay, we found that the best condition for the fungal isolates growth was on potato dextrose agar (PDA) media at 24°C temperature for 24 or 15 hr photoperiod. Leaf- stem and pods of soybean were inoculated by an atomizer with two isolates among three isolates to investigate PSD infection. In the result of two inoculation parts with two isolates, there was no significant difference in degree of pod infection and seed infection rate (%) between isolate2 and isolate3, but there was a tendency that pod inoculation than leaf- stem inoculation caused higher level of seed infection. These isolates obtained in this study would be applicable to screening of PSD resistant soybean germplasms in the breeding program.
Chien-Min Chen,Jae Hwan Lee,Meng-Yin Yang,Shang-Wun Jhang,Kai-Sheng Chang,Su-Wei Ou,Li-Wei Sun,Kuo-Tai Chen 대한척추신경외과학회 2023 Neurospine Vol.20 No.1
Objective: Sacroiliac joint (SIJ) pain is a common cause of chronic low back pain. Full-endoscopic rhizotomy of lateral branches of dorsal rami innervating SIJ is a potential option for patients’ refractory to medical treatment. The full-endoscopic rhizotomy is sometimes challenging under fluoroscopic guidance. This study is to evaluate the effectiveness of the navigation-assisted full-endoscopic rhizotomy for SIJ pain. Methods: The study was a retrospective match-paired study that enrolled consecutive patients undergoing navigation-assisted full-endoscopic rhizotomy for SIJ pain. The patient demographics, clinical outcomes, and operative parameters of endoscopic rhizotomy were compared with conventional cooled radiofrequency ablation (RFA) treatment. Results: The study enrolled 72 patients, including 36 patients in the endoscopic group. Thirty-six patients in the cooled RFA group were matched by age as the control. The follow-up time was at least 1 year. Patient characteristics were similar between the groups. The navigation-assisted endoscopic rhizotomy operation time was significantly longer than the cooled RFA. The visual analogue scale (VAS) for pain and Oswestry Disability Index (ODI) significantly decreased after each treatment. However, the between-group comparison revealed that the VAS and ODI of the patients after endoscopic rhizotomy were significantly lower than those after the cooled RFA group. There were no postoperative complications in the study. Conclusion: Navigation-assisted full-endoscopic rhizotomy is an alternative to SIJ pain treatment. Integrating intraoperative navigation can ensure accurate full-endoscopic rhizotomy to provide better durability of pain relief than the cooled RFA.
Chien-Tai Hong,Lung Chan,Chaur-Jong Hu,Chien-Min Lin,Chien-Yeh Hsu,Ming-Chin Lin 한국유방암학회 2017 Journal of breast cancer Vol.20 No.4
Purpose: Whether tamoxifen affects the risk of neurodegenerative disease is controversial. This nationwide population-based study investigated the risk of Parkinson’s disease (PD) associated with tamoxifen treatment in female patients with breast cancer using Taiwan’s National Health Insurance Research Database. Methods: A total of 5,185 and 5,592 female patients with breast cancer who did and did not, respectively, receive tamoxifen treatment between 2000 and 2009 were included in the study. Patients who subsequently developed PD were identified. A Cox proportional hazards model was used to compare the risk of PD between the aforementioned groups. Results: Tamoxifen did not significantly increase the crude rate of developing PD in female patients with breast cancer (tamoxifen group, 16/5,169; non-tamoxifen group, 11/5,581; p=0.246). Tamoxifen did not significantly increase the adjusted hazard ratio (aHR) for subsequently developing PD (aHR, 1.310; 95% confidence interval [CI], 0.605–2.837; p= 0.494). However, tamoxifen significantly increased the risk of PD among patients followed up for more than 6 years (aHR, 2.435; 95% CI, 1.008–5.882; p=0.048). Conclusion: Tamoxifen treatment may increase the risk of PD in Taiwanese female patients with breast cancer more than 6 years after the initiation of treatment.
An experimental study on creep deformation of thin-walled tubes under pure bending
Hsu, Chien-Min,Fan, Chun-Huei Techno-Press 2000 Structural Engineering and Mechanics, An Int'l Jou Vol.9 No.4
The creep deformation of pure bending (hold constant moment for a period of time) tests were conducted in this paper. Thin-walled tubes of 304 stainless steel were used in this investigation. The curvature-ovalization measurement apparatus, designed by Pan et al. (1998), was used for conducting the present experiments. It has been found that as soon as the creep deformation is started, the magnitudes of the tube curvature and ovalization of tube cross-section quickly increase. The magnitudes of the creep curvature and ovalization of tube cross-section increase fast with a higher hold moment than that with a lower one. Owing to the continuously increasing curvature during the creep deformation, the tube specimen buckles eventually.
Wang, Chien-Min,Chen, San-Yuan 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.2 No.e3
The structure evolution of perovskite phase Pb[(Mg<SUB>x</SUB>Zn<SUB>1-x</SUB>0<SUB>1</SUB>3/Nb<SUB>2</SUB>3/]O<SUB>3</SUB> (abbreviated as PZMN) was found to be highly dependent on its precursors' strong tendency of forming pyrochlore phase in the mixture of raw materials. Three major pyrochlore compositions, Pb<SUB>2</SUB>Nb<SUB>2</SUB>O<SUB>7</SUB>,Pb<SUB>3</SUB>Nb<SUB>2</SUB>O<SUB>8</SUB> and Pb<SUB>3</SUB>Nb<SUB>4</SUB>O<SUB>13</SUB>, were characterized and analyzed to understand their roles in the formation of perovskite PZMN phase. It was discovered that Pb<SUB>3</SUB>Nb<SUB>2</SUB>O<SUB>8</SUB> pyrochlore phase could catalyze the formation of perovskite phase in Pb-based relaxors. Based on phase structure evolution, a preparation method for forming high dielectric constant PZMN ceramics without using common stabilizers such as SrTiO<SUB>3</SUB> was proposed. With pre-synthesized perovskite PMN acting as stabilizer in the formation of Zn-rich PZMN having almost 100% perovskite content and a maximum dielectric constant of 22000. A ternaryphase diagram of PMN-PZN-SrTiO<SUB>3</SUB> was further constructed to investigate the perovskite develop,emt and its related electrical properties.
METAL COATING OF SAW DIAMOND GRIT BY FLUIDIZED BED
Sung, Chien-Min 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.1 No.2
Saw diamond grit can be effectively coated by metal powder to a large thickness using a simple sluidized bed system. By coating diamond with a suitable metal and heat treated to form a thin layer of carbide on the interface, the grit can be firmly held in saw segment. The result would be a lasting grit with high protrusion, hence, the was blade can cut more and faster. Moreover, the coating can enlarge the diamond grit and prevent it from segregating in the segment, therefore, the finished saw segment would be more uniform and reproducible.