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Integrated cryoEM imaging center at Seoul National University, Korea
Miji Jeon,Jun Bae Park,Hansol Lee,Gyuhee Kim,Eung Baek Kim,Mingyu Jung,Soung-Hun Roh 한국구조생물학회 2021 Biodesign Vol.9 No.4
The Center for Macromolecular and Cell Imaging (CMCI) is a core facility equipped with multiple cryo-capable electron microscopes including a 200 kV Thermofisher Glacios, 200 kV JEOL JEM2100Plus and 120 kV FEI TECNAI with direct electron detector technologies. The center also has auxiliary equipment for optimal specimen preparation, such as for protein purification, tissue culture, grid preparation and vitrification. The center offers to the scientific community the best practice of cutting-edge cryogenic electron microscopy (cryoEM) technology for specimen optimization, high-resolution data collection and structure determination. The center also develops the strategy of an integrated cryoEM imaging platform to visualize molecular architectures from purified proteins to cells.
Miji Lee,Duhak Yoon,전진태,어성국,강성호 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.11
A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 μm, outer diameter of 365 μm, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone (Mr = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) (Mr = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.
Miji Kim,김선영,Chang Won Won 대한노인병학회 2018 Annals of geriatric medicine and research Vol.22 No.2
Background: The new fall risk assessment (FRA) system is a composite and comprehen-sive assessment tool developed to predict the risk of falls. The aim of this pilot study was to examine the new FRA system’s test-retest reliability and sensitivity to change in commu-nity-dwelling older adults. Methods: This was an observational study with a test-retest de-sign and an 8-week fall prevention exercise program. A sample of 28 community-dwelling older adults with a mean age of 73.0 years (range, 65–80 years) participated in the study. The new FRA system was administered twice within a 7-day period for test-retest reliability expressed as intraclass correlation coefficient (ICC) and standard error of measurement (SEM) assessment. Eighteen subjects of them completed the 8-week fall prevention exer-cise intervention to evaluate the new FRA system’s sensitivity to change. Results: In the evaluation of interrater reliability for the new FRA system, the ICC (95% confidence inter-val) of the total score was 0.77 (0.47–0.98), with good reliability. The SEM was 11.61 for the total FRA score. A good to excellent reliability was observed, with ICC levels of 0.73 to 0.91 for the 4 composite scores of the new FRA system. Following the 8-week exercise intervention, the mean total FRA score (effect size, 0.58) significantly increased (p=0.028). Conclusion: The new FRA system has generally moderate to excellent interrater reliability and reliable sensitivity to change in community-dwelling older adults. Our findings provide support for the reliability of the new FRA system in healthy older adults without a fall his-tory. (Ann Geriatr Med Res 2018;22:80-87)
Mijie Kim,Yong Joo Park,Huiyeon Ahn,Byeonghak Moon,Kyu Hyuck Chung,Seung Min Oh 환경독성보건학회 2016 환경독성보건학회지 Vol.31 No.-
Objectives Aromatase inhibitors that block estrogen synthesis are a proven first-line hormonal therapy for postmenopausal breast cancer. Although it is known that standardized extract of Ginkgo biloba (EGb761) induces anti-carcinogenic effects like the aromatase inhibitors, the effects of EGb761 on steroidogenesis have not been studied yet. Therefore, the effects of EGb761 on steroidogenesis and aromatase activity was studied using a H295R cell model, which was a good in vitro model to predict effects on human adrenal steroidogenesis. Methods Cortisol, aldosterone, testosterone, and 17β-estradiol were evaluated in the H295R cells by competitive enzyme-linked immunospecific assay after exposure to EGb761. Real-time polymerase chain reaction were performed to evaluate effects on critical genes in steroid hormone production, specifically cytochrome P450 (CYP11/ 17/19/21) and the hydroxysteroid dehydrogenases (3β-HSD2 and 17β-HSD1/4). Finally, aromatase activities were measured with a tritiated water-release assay and by western blotting analysis. Results H295R cells exposed to EGb761 (10 and 100 μg/mL) showed a significant decrease in 17β-estradiol and testosterone, but no change in aldosterone or cortisol. Genes (CYP19 and 17β-HSD1) related to the estrogen steroidogenesis were significantly decreased by EGb761. EGb761 treatment of H295R cells resulted in a significant decrease of aromatase activity as measured by the direct and indirect assays. The coding sequence/Exon PII of CYP19 gene transcript and protein level of CYP19 were significantly decreased by EGb761. Conclusions These results suggest that EGb761 could regulate steroidogenesis-related genes such as CYP19 and 17β-HSD1, and lead to a decrease in 17β-estradiol and testosterone. The present study provides good information on potential therapeutic effects of EGb761 on estrogen dependent breast cancer.