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Biomedical Application of PHBV/Collagen (PHCP) Nanofiber as the Wound Dressing
Jin-Yi Han,Xu Zi Guang,Chan-Mi Moon,Jyung-Sik Kwak,Ki-Wan Oh,Han-Ik Bae 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.3
In this study, a nanofibrous scaffold was obtained by co-electrospinning poly (3-hydroxybutyrate- co-3-hydroxyvalerate) (PHBV) and collagen in 2,2,2-trifluoroethanol at a ratio of 3/7. The fiber diameters were in the range of 250-600 nm. It was found that PHBV/Collagen (PHCP) nanofibrous scaffold showed greater proliferation than the PHBV nanofibrous scaffold induced by oxidant in NIH3T3 cells. Otherwise, in the early-stage wound-healing mouse model, wound closure was evaluated according to wound size reduction and histology of regenerated skin on the backs of mice. Each of the tissues removed on day 0, 3, 6, 9, 12, 15, and 18 was used for analysis of biochemical and pathological changes. None of the nanofiber-attached mice showed significant difference on the third day, however, from the third day until the ninth day, significantly faster healing was observed in PHCP-attached mice, compared to control wounds in epithelialization, wound contraction, and histopathological examinations. These results strongly support the beneficial effects of biomedical application of PHCP nanofiber in acceleration of the initial phase of wound healing through α-SM actin contraction.
HACCP Performance of Employees in School Foodservice Operations and the Related Variables
Mi Kyeong Kim,Jyung Rewng Park,Myeonghwa Cha 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.4
The purpose of this study was to assess current food-handling practices of employees in school foodservice settings, as well as their knowledge levels, and identify relationships between knowledge, practices, and influencing variables. The survey was conducted for dietitians and employees in the school foodservice industry in Gyeongsangbuk-do province. A total of 270 and 570 questionnaires for dietitians and employees, respectively, were distributed by mail. Response rates were 62% (N=171) and 66% (N=376) from dietitians and employees, respectively. Data was analyzed using SPSS Windows (version 10.0). Descriptive statistics were used to summarize data. Pearson correlations were applied to test for relationships between knowledge and practice of HACCP principles. Stepwise regression analysis was performed to examine the influence of knowledge, current education guidelines, demographic information (working experience, academic background, and certification for food and cooking), and school characteristics (food production system, service style, and number of meals). School foodservice employees were found to have a significant amount of food safety knowledge (67.5±1.8 out of 100 possible points). Proper food handling practices were not always being followed in many schools. The relationship between their knowledge, current HACCP education training, and food handling practices was not significant. These results suggested the present situation of HACCP trainings performed by dietitians were inadequate for many school foodservice operations. The number of meals in school was an independent predictor of the employees’ food-handling practices. These results suggest that an effective education program should integrate endeavors that take account of social and environmental influences on food safety to support the improvement of food-handling practices and the implementation of a HACCP program. Furthermore, dietitians should continue to provide consulting, training, and technical assistance to schools on HACCP implementation.
김중명,김재식,전효진,박은미,서장수,김경숙 慶北大學校 醫科大學 1984 慶北醫大誌 Vol.25 No.3
경북대학교 의과대학 부속병원 임상병리과에서 1981년 1월부터 1984년 12월까지 임상재료로부터 분리된 E. coli 555주(1981년에 50주, 1982년에 105주, 1983년에 97주 및 1984년에 303주)를 임의로 선택하여 disk diffusion method로, 이 중 각 년도별로 다시 50주를 임의로 선택하여 plate dilution method로 Ap, Ce, Cm, Tc, Ak, Gm 및 Tb에 대한 감수성과 그의 연차적 추이를 보았고, 그 내성균주에 있어서는 검체별 분포, 연차적증감 및 다체내성과 그 검체별 분포를 보아 다음과 같이 요약할 수 있었다. Ap, Ce, Cm, Tc, Ak, Gm 및 Tb에 대한 감수성에 있어서는 disk diffusion method의 경우에는 Ak에서 가장 높았고 Tc에 가장 낮았으며, 년차적 증감은 큰 변화가 없었으며 plate dilution method의 경우에는 Ak에서 가장 높았고 dilution method의 경우에는 Ak에서 가장 높았고 Ap에 가장 낮았으며 년차적 증강은 대체로 감소하는 추세였다. 내성균주의 재료별 분포를 보면 뇨, 농 및 객담 및 기관지분비물에서 높았으며 그 출현율도 그 재료에서 높았으며 다제내성은 1제에서 7제에서 걸쳐있었는데, 7제내성균주가 16주 있었으며, 특히 소변 유례의 7제내성균주는 9주나 있었다. The bacteria tested were 555 strains of Escherichia coli(E. coli, 1981-50 strains, 1982-105 strains, 1983-97 strains and 1984-303 strains), and were isolated in our clinical laboratory from Jan. 1981 to Dec. 1984. The antimicrobial susceptibility agains 7 antimicrobial agents, which composed of ampicillin, cephalothin, chloramphenicol, tetracycline, amikacin, gentamicin and tobramycin, were perfomed by means of a single high potency disk diffusion test and a plate dilution test. E. coli showed the highest sensitivity to amikacin as 93-96% by disk diffusion method and 70-92% by plate dilution method, and the lowest sensitivity to tetracycline as 18-23% by disk diffusion method and to ampicillin as 8-24% by plate dilution method, and relatively high sensitivity to gentamicin and tobramycin and relatively low sensitivity to chloramphenicol by both methods. The susceptible strains decreased by year which were tested by plate dilution method but not incresed or decresed by disk diffusion method. The resistant strains of E. coli were distributed mainly in blood, urine, pus, sputum and bronchial secrete, and the rates of appearance were all high in above specimens, and multiple drugs resistance of E. coli were related up to seven drugs, and there were 16 strains which were resistant agains 7 agents above mentioned and especialy high distributed in urine as 9 strains.
이미랑(Mi-Rang Lee),도경탁(Kyoung-Tag Do),한정주(Jyung-Ju Han),문소현(So-Hyun Moon),강한석(Han-Seok Kang),김선구(Seon-Ku Kim),신택순(Teak-Soon Shin),이홍구(Hong-Goo Lee),황대연(Dae-Yon Hwang),김용균(Yong-Gyun Kim),손시환(Sea-Hwan Soh 한국생명과학회 2009 생명과학회지 Vol.19 No.10
Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and ?Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.
HACCP Performance of Employees in School Foodservice Operations and the Related Variables
Kim, Mi-Kyeong,Park, Jyung-Rewng,Cha, Myeong-Hwa The Korean Society of Food Science and Nutrition 2005 Preventive Nutrition and Food Science Vol.10 No.4
The purpose of this study was to assess current food-handling practices of employees in school food service settings, as well as their knowledge levels, and identify relationships between knowledge, practices, and influencing variables. The survey was conducted for dietitians and employees in the school foodservice industry in Gyeongsangbuk-do province. A total of 270 and 570 questionnaires for dietitians and employees, respectively, were distributed by mail. Response rates were $62\%$ (N=171) and $66\%$ (N=376) from dietitians and employees, respectively. Data was analyzed using SPSS Windows (version 10.0). Descriptive statistics were used to summarize data. Pearson correlations were applied to test for relationships between knowledge and practice of HACCP principles. Stepwise regression analysis was performed to examine the influence of knowledge, current education guidelines, demographic information (working experience, academic background, and certification for food and cooking), and school characteristics (food production system, service style, and number of meals). School foodservice employees were found to have a significant amount of food safety knowledge ($67.5\pm1.8$ out of 100 possible points). Proper food handling practices were not always being followed in many schools. The relationship between their knowledge, current HACCP education training, and food handling practices was not significant. These results suggested the present situation of HACCP trainings performed by dietitians were inadequate for many school foodservice operations. The number of meals in school was an independent predictor of the employees' food-handling practices. These results suggest that an effective education program should integrate endeavors that take account of social and environmental influences on food safety to support the improvement of food-handling practices and the implementation of a HACCP program. Furthermore, dietitians should continue to provide consulting, training, and technical assistance to schools on HACCP implementation.
ZnO 나노구조물 표면 처리를 통한 유무기 복합체 태양전지의 특성 향상
진미진(Jin, Mi-Jin),이정환(Lee, Jyung-Hwan),반태호(Ban, Tae-Ho),김상우(Kim, Sang-Woo),정순욱(Jeong, Soon-Wook),김성진(Kim, Sung-Jin) 한국신재생에너지학회 2009 한국신재생에너지학회 학술대회논문집 Vol.2009 No.11
유기물 기반 태양전지의 낮은 전하수송 효율 문제(전하이동거리 약 ~20nm)를 개선하기 위해서는 생성된 전자-홀 쌍이 빠르게 전극 층으로 이동하도록 태양전지 의 층 구조 및 특성을 제어하는 것이 중요하다. 그 방안으로 무기물 반도체인 산화아연을 나노구조물 형태로 제어하여 전자 이동층(Electron Conductive Layer) 으로 도입, 생성된 전자의 이동 가능한 면적을 넓히고 전자수송효율을 높여 유무기 복합체 태양전지의 Fill Factor를 향상시켰다. 또한 제조된 산화아연 나노구조물의 산소플라즈마 처리와 같은 표면 처리를 통하여 유기물 층과의 흡착성을 높이고 나노구조물 표면에 oxygen을 침투시켜 전자 이동도를 향상시켰다.