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Genetic and phylogenetic analysis of the honey bee sacbrood virus from jiangxi isolates
Meng Yaping,Yu Xinyue,Huang Qiang,Zhang Li Zhen,Wu Xiaobo,Wang Zilong,Yan Wei Yu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1
The high prevalence of honeybee viral diseases poses a severe threat to the health of honeybees and causes substantial economic losses worldwide. Sacbrood virus (SBV) is a single-strand RNA virus that infects honeybees at all life stages. The infection can shorten the lifespan of adult bees and is lethal to larvae. SBV is the major cause of honeybee losses in Asia. Genetic and phylogenetic analyses of SBV isolates from different areas have been previously conducted. However, the impact of Apis mellifera Linnaeus and Apis cerana Fabricius coexistence on the infection and phylogeny of SBV remains unknown. In this study, we collected A. cerana and A. mellifera samples from commercial apiaries, only A. cerana in mountainous region. SBV prevalence was evaluated in three commercial apiaries of Jinxi, Tonggu and Nanchang and two mountainous regions of Zixi and Yifeng. In our sampling location, we found a higher SBV prevalence in the mountainous regions than in commercial apiaries. Partial structural polyprotein coding sequences were sequenced and compared with other GenBank SBV isolates. Phylogenetic tree topologies showed that SBV isolates form two major groups based on their host specificity, and isolates from same country tend to cluster together in subclades, indicating that the host and geographic region has significant effects on SBV strain specificity.
Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8
Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.
Yu-Lin Dai,Meng-Dan Qiao,Peng Yu,Fei Zheng,Hao Yue,Shu-Ying Liu 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2
Background: This article aims to compare and analyze the contents of ginsenosides in ginseng ofdifferent plant ages from different localities in China. Methods: In this study, 77 fresh ginseng samples aged 2e4 years were collected from 13 differentcultivation regions in China. The content of eight ginsenosides (Rg3, Rc, Rg1, Rf, Rb2, Rb1, Re, and Rd) wasdetermined using rapid resolution liquid chromatography coupled with quadrupoleetime-of-flighttandem mass spectrometry (RRLC-Q-TOF MS/MS) to comparatively evaluate the influences of cultivationregion and age. Results: Ginsenoside contents differed significantly depending on age and cultivation region. The contentsof ginsenosides Re, Rc, Rg1, Rg3, and Rf increased with cultivation age, whereas that of ginsenoside Rb1peaked in the third year of cultivation. Moreover, the highest ginsenoside content was obtained fromChangbai (19.36 mg/g) whereas the lowest content was obtained from Jidong (12.05 mg/g). Ginseng fromJilin Province contained greater total ginsenosides and was richer in ginsenoside Re than ginseng of thesame age group in Heilongjiang and Liaoning provinces, where Rb1 and Rg1 contents were relatively high. Conclusion: In this study, RRLC-Q-TOF MS/MS was used to analyze ginsenoside contents in 77 ginsengsamples aged 2e4 years from different cultivation regions. These patterns of variation in ginsenosidecontent, which depend on harvesting location and age, could be useful for interested parties to chooseginseng products according to their needs.
In Vitro and In Vivo Evaluation of Ranitidine Hydrochloride Loaded Hollow Microspheres in Rabbits
Yu-meng Wei,Ling Zhao 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10
The objective of this investigation was to develop the hollow microspheres as a new dosage form of floating drug delivery systems with prolonged stomach retention time. Hollow microspheres containing ranitidine hydrochloride (RH) were prepared by a novel solvent diffusion-evaporation method using ethyl cellulose (EC) dissolved in a mixture of ethanol and ether (6:1.0, v/v). The yield and drug loading amount of hollow microspheres were 83.21±0.28% and 20.71±0.32%, respectively. The in vitro release profiles showed that the drug release rate decreased with increasing viscosity of EC and the diameter of hollow microspheres, while increased with the increase of RH/EC weight ratio. Hollow microspheres could prolong drug release time (approximately 24 h) and float over the simulate gastric fluid for more than 24 h. Pharmacokinetic analysis showed that the bioavailability from RH-hollow microspheres alone was about 3.0-times that of common RH gelatin capsules, and it was about 2.8-times that of the solid microspheres. These results demonstrated that RH hollow microspheres were capable of sustained delivery of the drug for longer period with increased bioavailability.
타오바오 라이브 스트리밍 쇼핑의 사용 의도에 영향을 미치는 요인: 몰입이론 및 합리적 행동 이론을 기반으로
( Meng-ying Yu ),문병준 ( Byeong-joon Moon ) 한국국제경영학회 2021 國際經營硏究 Vol.32 No.3
본 연구는 타오바오 라이브 스트리밍 쇼핑의 특성과 주관적 규범이 라이브 스트리밍 쇼핑 사용 의도에 어떤 영향을 미치는지에 대해 조사하였다. 타오바오 라이브 스트리밍 쇼핑의 특성인 ‘인식된 즐거움’, ‘신뢰성’, ‘상호작용성’, ‘즉시성’과 ‘주관적 규범’이 타오바오 라이브 스트리밍 쇼핑의 사용 의도에 미치는 영향을 파악하기 위해 몰입이론과 합리적 행동 이론을 기반으로 연구를 진행하였다. 중국인 335명을 대상으로 설문조사를 실시하였으며 결과는 다음과 같다. ‘상호작용성’을 제외한 나머지 특성인 ‘인식된 즐거움’, ‘신뢰성’ 및 ‘즉시성’이 타오바오 라이브 스트리밍 쇼핑 사용에 대한 태도에 긍정적인 영향을 미치는 것으로 나타났다. 타오바오 라이브 스트리밍 쇼핑 사용에 대한 태도는 타오바오 라이브 스트리밍 쇼핑 사용 의도에 긍정적인 영향을 미치는 것으로 나타났으며 ‘주관적 규범’이 사용 의도에 긍정적인 영향을 줄 수 있는 것으로 나타났다. 본 연구는 타오바오 라이브 스트리밍 쇼핑의 특성과 ‘주관적 규범’이 라이브 스트리밍 쇼핑 이용 의도에 어떤 영향을 미치는지 이해하는데 도움이 될 뿐만 아니라 다양한 라이브 스트리밍 쇼핑에 관한 마케팅 전략을 개발하는데 도움이 될 수 있다. The purpose of this study is to examine how the characteristics of TLSS (Taobao live streaming shopping) and subjective norm influence live streaming shopping usage intentions in e-commerce. We apply the flow theory and the TRA (Theory of reasoned action) to investigate the effects of perceived enjoyment, trust, interactivity, immediacy on attitude toward using TLSS, and the impact of subjective norm on intention to use TLSS. The research sample consisted of 335 respondents from China. The results show that interactivity is not significantly related to attitude toward using TLSS while perceived enjoyment, trust, and immediacy are significantly related to attitude toward using TLSS which in turn, indicate consumer’s usage intention of TLSS. Also, Subjective norm has a positive impact on TLSS usage intention. Our finding not only helps the researchers understand how the characteristics of TLSS and subjective norm influence live streaming shopping usage, but also assists the streamers and vendors in developing the better live streaming marketing strategy.
Meng, Xin-Yu,Liu, Juan,Lv, Feng,Liu, Ming-Qiu,Wan, Jing-Ming Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Objective: To investigate the correlation between extracellular matrix protein-1 (ECM1) and the growth, metastasis and angiogenesis of laryngeal carcinoma. Materials and Methods: Forty-five samples with laryngeal benign and malignant tumors confirmed by pathology in Laiwu City People's Hospital from March 2006 to March 2011 were collected, in which there were 29 cases with laryngeal carcinoma and 16 with benign tumors. The expression of ECM1 and factor VIII-related antigens in patients with laryngeal carcinoma and those with benign tumors was respectively detected using immunohistochemical method, and the correlation between ECM1 staining grade and microvessel density (MVD) was analyzed. Results: In laryngeal carcinoma tissue, ECM1 was mainly expressed in cytoplasm, less in cytomembrane or intercellular substance. With abundant expression in the tissue of laryngeal benign tumors (benign mesenchymoma and hemangioma), ECM1 was primarily expressed in the connective tissue, which was different from the expression in laryngeal carcinoma tissue. The proportion of positive ECM1 staining (++) in patients with laryngeal carcinoma was dramatically higher than those with benign tumors (p<0.05), and that of strongly-positive ECM1 staining (+++) slightly higher. The results of Spearman nonparametric correlation analysis revealed that ECM1 staining grade in laryngeal carcinoma tissue had a significantly-positive correlation with MVD (r=0.866, p=0.000). Conclusions: ECM1 expression in laryngeal carcinoma is closely associated with tumor cell growth, metastasis and angiogenesis, which can be considered as an effective predictor in the occurrence and postoperative recurrence of laryngeal carcinoma.