Verification of Wisteria floribunda agglutinin-positive glycoproteins as a cholangiocarcinoma marker

Atsushi Matsuda,Atsushi Kuno,Hideki Matsuzaki,Toru Kawamoto,Toshihide Shikanai,Yasuni Nakanuma,Masakazu Yamamoto,Nobuhiro Ohkohchi,Yuzuru Ikehara,Junichi Shoda,Jun Hirabayashi,Hisashi Narimatsu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Cholangiocarcinoma (CC) is a lethal malignancy which exhibits asymptomatic growth infiltrating the surrounding structures, and thus,CC is usually detected at an advanced stage. The mainstay of treatment for CC is complete resection with negative surgical margins. Therefore, its diagnosis at a relatively early stage is demanded for performing the surgical resection. Since the definitive CC diagnosis relies on invasive methods such as biliary cytology and biopsy, a noninvasive assay with high diagnostic accuracy is keenly required. In the previous meeting, we reported that Wisteria floribunda agglutinin (WFA) is the best probe lectin which reliably distinguishes between CC and normal bile duct epithelia in tissue sections. Moreover, L1 cell adhesion molecule (L1CAM), CA125, and maspin were assigned as the reliable CC marker candidates by WFA-assisted glycoproteomics and immunohistochemistry. In this meeting, we will introducethe verification and validation process in one of the above candidates, L1CAM. Since the serum concentration of L1CAM was low as described in other reports (< 5 ng/mL), we firstly constructed a highly-sensitive detection system to confirm the existence of L1CAM in both bile and serum of CC patients with immunoprecipitation and western blotting. We then performed highly-sensitive glycan profiling with antibody-assisted lectin microarray (limit of detection: 25 pg) and confirmed WFA-positivity of biliary L1CAM from the CC patients. The subsequent validation study using bile samples from CC patients (n = 29) and patients with benign bile duct diseases (n = 29) showed that WFA-positive L1CAM distinguished CC from the benigndiseases with good specificity (sensitivity = 0.66, specificity = 0.93, overall accuracy = 0.79, area under the receiver operating curve [AUC] = 0.82). The combined use of the L1CAM assay with the highly-sensitive assay detecting WFA-positive sialylated mucin 1 (WFA-sialyl MUC1), a reliable CC marker (Matsuda A., et al., Hepatology, 2010), sufficiently improved the diagnostic accuracy of CC (overall accuracy = 0.84, AUC = 0.93). This combination assay using WFA–L1CAM and WFA–sialyl MUC1 will possibly be a useful serological test with enhanced reliability.

A Method for Embedding Information into Printed Documents using Dot Pattern Watermarking

Junichi MATSUDA,Tomoya MIWA,Ken MATSUDA,Yoshiki MIZUKAMI,Katsumi TADAMURA 대한전자공학회 2009 ITC-CSCC :International Technical Conference on Ci Vol.2009 No.7

We propose a method for embedding information into printed documents. The method is using dot pattern watermarking based on illustration so that impression of documents is natural. Since random dots are used for conventional dot pattern, documents with dot pattern often give strange impression to readers. Our method is to use dot pattern image transformed from illustration image. We developed two key techniques; one is a method for transforming illustration image to dot pattern image suitable for watermarking; another one is a method for embedding information that can reduce noise caused by errors when printing and scanning documents. We verified the impression of printed documents with dot pattern generated by the proposed method by subjective experiments.

UV-responsive intracellular signaling pathways: MAPK, p53, and their crosstalk

Matsuda, Naoki Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

There are two distinct UV-responsive signaling pathways in UV-irradiated mammalian cells, i.e., the DNA damage-dependent and -independent pathways. The former occurs in nucleus and results in growth arrest and apoptosis via post-translational modification of p53. The latter is initiated by oxidative stress and/or by damages in cell membrane or cytoplasm, which activate signaling cascade through intracellular molecules including mitogen activated protein kinases (MAPK). In normal human fibroblastic cells, all of MAPK family members, extracellular signal-related kinases (ERK), c-Jun N-terminal kinases (JNK) and p38, were rapidly phosphorylated following UV-irradiation. ERK phosphorylation was suppressed by an inhibitor of receptor tyrosine kinases (RTK). As ERK usually responds to mitogenic stimuli from RTK ligands, UV-induced ERK phosphorylation may be linked to the proliferation of survived cells. In contrast, phosphorylation of JNK and p38, as well as apoptosis, were modulated by the level of UV-generated oxidative stress Therefore, JNK and p38 may take part in oxidative stress-mediated apoptosis. Phosphorylation of p53 at Ser and Thr residues are essential for stabilization and activation of p53. Among several sites reported, we confirmed phosphorylation at Ser-15 and Ser-392 after UV-irradiation. Both of these were inhibited by a phosphoinositide 3-kinase inhibitor, presumably due to the shutdown of signals from DNA damage to p53. Phosphorylation at Ser-392 was also sensitive to an antioxidant and a p38 inhibitor, suggesting that Ser-392 of p53 is one of the possible points where DNA damage-dependent and -independent apoptic signals merge. Thus, MAPK pathway links UV-induced intracellular signals to the nuclear responses and modifies DNA damage-dependent cellular outcome, resulting in the determination of cell death.

Application of Composites Composed of Phosphoric Acid-Doped Silica Gel and Styrene-Ethylene-Butylene-Styrene Elastomer to Electric Double-Layer Capacitors

Matsuda, Atsunori,Honjo, Hiroshi,Hirata, Kazuki,Tatsumisago, Masahiro,Minami, Tsutomu The Korean Ceramic Society 1999 The Korean journal of ceramics Vol.5 No.4

Highly proten-conductive elastic composites have been successfully prepared from $H_3PO_4$-doped silica gel and styrene-ethylene-butylene-styrene block elastic copolymer. In addition solid state electric double-layer capacitors have been fabricated using the composite as an electrolyte and activated carbon powders(ACP) hybridized with the composite as a polrizable electrode. The cyclic voltammogram of the electric double-layer capacitor fabricated demonstrated that electric charge was stored in the elecric double-layer at the interface between the polarizable electrode and the electrolyte. The value of capacitance of the capacitor was 10 F/(gram of total ACP), which was comparable to that of the capacitors using conventional liquid electrolytes.

Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA

Matsuda, Daiki,Hosoda, Nao,Kim, Yoon Ki,Maquat, Lynne E Nature Publishing Group 2007 Nature Structural and Molecular Biology Vol.14 No.10

Nonsense-mediated mRNA decay (NMD) generally eliminates messenger RNAs that prematurely terminate translation and occurs in all eukaryotes that have been studied, although with mechanistic variations. In mammals, NMD seems to be restricted to newly synthesized mRNA that is bound by the cap-binding heterodimer CBP80-CBP20 (CBP80/20) and typically has at least one exon junction complex (EJC) situated downstream of the nonsense codon and added post-splicing. However, mammalian NMD can also target spliced mRNA lacking an EJC downstream of the nonsense codon. Here we provide evidence that this additional pathway, known as failsafe NMD, likewise seems to be restricted to CBP80/20-bound mRNA and does not detectably target its subsequently remodeled product, eIF4E-bound mRNA. Our studies, including analyses of factor dependence, reveal important shared features of the two mammalian-cell NMD pathways as well as fundamental differences between NMD in mammals and Saccharomyces cerevisiae.

Physiology, pathogenicity and immunogenicity of <i>lon</i> and/or <i>cpxR</i> deleted mutants of <i>Salmonella</i> Gallinarum as vaccine candidates for fowl typhoid

Matsuda, Kiku,Chaudhari, Atul A.,Kim, Sam Woong,Lee, Kyeong Min,Lee, John Hwa EDP Sciences 2010 VETERINARY RESEARCH Vol.41 No.5

<P>To construct a novel live vaccine candidate for fowl typhoid (FT) caused by <I>Salmonella</I> Gallinarum (SG), the <I>lon</I> and <I>cpxR</I> genes that are related to host-pathogen interaction were deleted from a wild type SG using the allelic exchange method. The mutants were grown normally, as was the wild type. The biochemical properties of the mutants remained very similar to those of the wild-type, while JOL914 (Δ<I>lon</I>) and JOL916 (Δ<I>lon</I>Δ<I>cpxR</I>) were mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, and 46.2-fold in JOL914, JOL915 (Δ<I>cpxR</I>), and JOL916, respectively. Dot-blot analysis demonstrated significant increases of FimA expression at 6.77-, 2.33-, and 3.90-fold for JOL914, JOL915, and JOL916, respectively. Internalizations of JOL914, JOL915, and JOL916, in chicken abdominal macrophages, were increased at 4.65-, 0.50-, and 2.72-fold, respectively. Virulences of JOL914, JOL915 and JOL916, analyzed by LD<SUB>50</SUB> using 1-week-old chickens, were attenuated approximately at 10<SUP>1</SUP>-, 10<SUP>1</SUP>-, and > 10<SUP>3</SUP>-fold, respectively. The oral inoculations of 2 × 10<SUP>7</SUP> cfu of the wild type, JOL914, JOL915 and JOL916 caused 55.6, 16.7, 22.2, and 0.0% mortality, respectively. Significantly moderate gross lesions of the liver and spleen were observed in the JOL916 group compared to the other groups. An induced immune response and significant peripheral mononuclear proliferation reaction were observed in the JOL916 group. At the protection against the wild type challenge, JOL916 offered 100% protection. Thus, the results of this study suggest that JOL916 among the mutants studied represented the safest and most effective live vaccine candidate against FT.</P>

Mechanical Properties of Injection Molded Fe-6% Ni-0.4% C Steels with Varying Mo Contents of 0.5 to 2 %

Matsuda, Mitsuhiro,Miura, Hideshi 대한금속재료학회 2003 METALS AND MATERIALS International Vol.9 No.6

In our previous studies, sintered and heat-treated alloy steels (Fe-6Ni-0.5Mo-0.4C (mass%)) produced by a MIM process showed excellent mechanical properties of 2000 MPa tensile strength and 5 % elongation. This was attributed to the solid solution strengthening and the mezzo-heterogeneous microstructure, which consisted of martensite or retained austenite (Ni and Mo rich phases) surrounded by a network of tempered martensite. This study has been performed to clarify the effect of Mo on the mezzo-heterogeneous microstructure and the mechanical properties of MIM processed and sintered alloy steels (Fe-6Ni-0.4C) with varying Mo content (0.5~2 mass%). The tensile properties of the heat-treated steels with added 2 mass% Mo were lower than those of the steels with added 0.5 mass% Mo. The reduction in the tensile properties, particularly the appearance of large pores formed at the original location of Mo powder through the transient liquid phase formation and the low hardness of the matrix, was due to the low sintered density. By using mechanically milled fine Ni and Mo powders, the heat-treated steel (Fe-6Ni-2Mo-0.4C) showed excellent properties, including tensile strength of 1800 MPa and ductility of 2.2 % elongation.

Frustrated minority spins in GeNi₂O₄

Matsuda, M.,Chung, J.-H.,Park, S.,Sato, T. J.,Matsuno, K.,Aruga Katori, H.,Takagi, H.,Kakurai, K.,Kamazawa, K.,Tsunoda, Y.,Kagomiya, I.,Henley, C. L.,Lee, S.-H. Editions de Physique 2008 Europhysics letters Vol.82 No.3

<P>Recently, two consecutive phase transitions were observed, upon cooling, in an antiferromagnetic spinel GeNi<SUB>2</SUB>O<SUB>4</SUB> at <I>T</I><SUB><I>N</I>1</SUB>=12.1 K and <I>T</I><SUB><I>N</I>2</SUB>=11.4 K, respectively (Crawford M. K. <I>et al</I>., <I>Phys. Rev. B</I>, <B>68</B> (2003) 220408(R)). Using unpolarized and polarized elastic neutron scattering we show that the two transitions are due to the existence of frustrated minority spins in this compound. Upon cooling, at <I>T</I><SUB><I>N</I>1</SUB> the spins on the ⟨111⟩ kagome planes order ferromagnetically in the plane and antiferromagnetically between the planes (phase I), leaving the spins on the ⟨111⟩ triangular planes that separate the kagome planes frustrated and disordered. At the lower <I>T</I><SUB><I>N</I>2</SUB>, the triangular spins also order in the ⟨111⟩ plane (phase II). We also present a scenario involving exchange interactions that qualitatively explains the origin of the two purely magnetic phase transitions.</P>

Surface and Electronic Properties of Hydrogen Terminated Si [001] Nanowires

Matsuda, Yuki,Tahir-Kheli, Jamil,Goddard , William A. American Chemical Society 2011 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.115 No.25

<P>The calculated band gaps reported previously for silicon nanowires (SiNW) have disagreed with the experimental values both in magnitude and in the behavior with radius. We resolve this discrepancy here. We report ab initio quantum mechanical calculations of hydrogen terminated Si [001] nanowires (H–SiNWs) as a function of diameter (<I>d</I>) and hydrogen coverage using the B3LYP density functional. For smaller diameters (<I>d</I> ≤ 1.9 nm) we find that the most stable surface is fully saturated with hydrogen leading to direct band gaps. For larger diameters, the surface dangling bonds are not saturated, leading to surface LUMO and HOMO states that lower the gap and lead to an indirect band gap. This transition from direct to indirect gap resolves the previous disagreement in the scaling of band gap with diameter. We conclude that the electronic properties of Si NW depend sensitively on controlling the diameter and surface hydrogen coverage.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2010/jpccck.2010.115.issue-25/jp106048u/production/images/medium/jp-2010-06048u_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp106048u'>ACS Electronic Supporting Info</A></P>

Avian colibacillosis caused by an intestinal pathogenic <i>Escherichia coli</i> isolate from calf diarrhea

Matsuda, Kiku,Chaudhari, Atul A.,Lee, John Hwa Elsevier 2010 Research in veterinary science Vol.89 No.2

<P><B>Abstract</B></P><P>An intestinal pathogenic <I>Escherichia coli</I> isolate from calf diarrhea, containing the <I>iutA</I>, <I>f17A</I>, <I>afa</I>-<I>8D</I>, and <I>cnf2</I> genes, was able to cause avian colibacillosis after experimental infection in chickens. Intra-tracheal inoculation and spray of this strain caused 10% of mortality and gross lesions, including airsacculitis, pericarditis, and perihepatitis. These results suggest that some bovine pathogenic <I>E. coli</I> can cause extra-intestinal infections in other animal species.</P>