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New tricyclic systems as photosensitizers towards triple negative breast cancer cells
Marilia Barreca,Angela Maria Ingarra,Maria Valeria Raimondi,Virginia Spanò,Antonio Palumbo Piccionello,Michele De Franco,Luca Menilli,Valentina Gandin,Giorgia Miolo,Paola Barraja,Alessandra Montalbano 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.11
Nineteen pyrrolo[1,2- h ][1,7]naphthyridinonesand pyrido[2,3- c ]pyrrolo[1,2- a ]azepinones were synthesizedas new tricyclic systems in which the pyridine ringis annelated to the 6,7-dihydroindolizin-8(5 H )-one and5,6,7,8-tetrahydro-9 H -pyrrole[1,2- a ]azepine-9-one moietiesto obtain potential photosensitizing agents. They were testedfor their photoantiproliferative activity on a triple-negativebreast cancer cell line, MDA-MB-231, in the dark and underUVA light (2.0 J/cm 2 ). We demonstrated that their toxicity,only when exposed to light, was primarily due to the generationof reactive oxygen species while their photodegradationproducts were not responsible for their activity. The mostactive compounds exhibited photocytotoxicity with IC 50 valuesat low micromolar level inducing a decrease in the intracellularcontent of thiol, thus triggering cancer cell deaththrough apoptosis. All the pyridone derivatives revealed tobe pure photosensitizers with preferential photocytotoxicactivity towards cancerous over healthy cells. Altogether,the results obtained confi rm pyrrolo[1,2- h ][1,7]naphthyridinonesand pyrido[2,3- c ]pyrrolo[1,2- a ]azepinones as promisingphotosensitisers against triple-negative breast cancer.
Luiz Carlos Borges,Marilia Braz Botelho 국립민속박물관 2008 International Journal of Intangible Heritage Vol.3 No.-
The intangible heritage is not easy to present in amuseum exhibition, and this is perhaps especially so inthe case of what the 2003 UNESCO Intangible HeritageConvention terms ‘knowledge and practices concerningnature and the universe’. The Brazilian Federal Museumof Astronomy and Related Sciences (MAST), Rio deJaneiro, initiated an exhibition and educationalprogramme on this theme for International MuseumsWeek in 2004, focusing particularly on four very differentcosmologies (i.e. narratives that attempt to explain theorigin of the Universe): the Biblical story in Genesis, thecontemporary scientific ‘Big Bang’ theory, and thecreation stories of two Brazilian indigenous populations:the Tukano people of the Amazon Region, and the Guaraniof southern Brazil and some neighbouring countries. Theevent, called Myths of Origin - man and hiscomprehension of the Universe and of the planet on whichhe lives, consisted of a conceptual, sensory andeducational experience whose principal objective was tochallenge preconceptions while questioning also thevisitors’ perceptions which arise from an educationalsystem where many of these ideas are taught as absolutetruths. Following Paulo Freire’s theory of learning and anon-restrictive understanding of the sciences, we choseto present the four different narratives on an equal basisand invited the visitor to explore these withoutpreconceptions. The underlying objective was to establisha dialogue among these diverse discourses about thecosmos, which we hoped would encourage visitors to takea critical view of the sciences and the way they areinterpreted in museums.
Yamada, Ana Lucia M.,Pinheiro, Marcelo,Marsiglia, Marilia F.,Hagen, Stefano Carlo F.,Baccarin, Raquel Yvonne A.,da Silva, Luis Claudio L.C. The Korean Society of Veterinary Science 2020 JOURNAL OF VETERINARY SCIENCE Vol.21 No.2
Background: Physical exercise is known to cause significant joint changes. Thus, monitoring joint behavior of athletic horses is essential in early disorders recognition, allowing the proper management. Objectives: The aims of this study were to determine the morphological patterns, physical examination characteristics and ultrasound findings of show jumping horses in training and to establish a score-based examination model for physical and ultrasound follow-ups of metacarpophalangeal joint changes in these animals. Methods: A total of 52 metacarpophalangeal joints from 26 horses who were initially in the taming stage were evaluated, and the horses' athletic progression was monitored. The horses were evaluated by a physical examination and by B-mode and Doppler-mode ultrasound examinations, starting at time zero (T0), which occurred concomitantly with the beginning of training, and every 3 months thereafter for a follow-up period of 18 months. Results: The standardized examination model revealed an increase in the maximum joint flexion angles and higher scores on the physical and ultrasound examinations after scoring was performed by predefined assessment tools, especially between 3 and 6 months of evaluation, which was immediately after the horses started more intense training. The lameness score and the ultrasound examination score were slightly higher at the end of the study. Conclusions: The observed results were probably caused by the implementation of a training regimen and joint adaptation to physical conditioning. The joints most likely undergo a pre-osteoarthritic period due to work overload, which can manifest in a consistent or adaptive manner, as observed during this study. Thus, continuous monitoring of young athlete horses by physical and ultrasound examinations that can be scored is essential.
Hepatoprotective Effect of Essential Oils from Hyptis crenata in Sepsis-Induced Liver Dysfunction
Glauber Cruz Lima,Yuri de Abreu Gomes Vasconcelos,Marilia Trindade de Santana Souza,Alan Santos Oliveira,Rangel Rodrigues Bomfim,Ricardo Luiz Cavalcanti de Albuquerque Junior,Enilton Aparecido Camargo 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.7
No specific therapeutics are available for the treatment of sepsis-induced liver dysfunction, a clinical complication strongly associated with the high mortality rate of septic patients. This study investigated the effect of the essential oil of Hyptis crenata (EOHc), a lamiaceae plant used to treat liver disturbances in Brazilian folk medicine, on liver function during early sepsis. Sepsis was induced by the cecal ligation and puncture (CLP) model. Rats were divided into four groups: Sham, Sham+EOHc, CLP, and CLP+EOHc. EOHc (300 mg/kg) was orally administered 12 and 24 h after surgery. The animals were sacrificed for blood collection and liver tissue samples 48 h after surgery. Hepatic function was evaluated by measuring serum bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase, and alanine aminotransferase (ALT) levels. The levels of malondialdehyde and the activity of superoxide dismutase, catalase, and GSH peroxidase (GSH-Px) were measured for assessment of oxidative stress. Liver morphology was analyzed by hematoxylin and eosin staining. EOHc normalized serum ALP, ALT, and bilirubin levels and inhibited morphological changes. In addition, we observed that EOHc inhibited elevation in hepatic lipid peroxidation and reduction of the glutathione peroxidase activity induced by sepsis. Our data show that EOHc plays a protective effect against liver injury induced by sepsis.
Trametes villosa Lignin Peroxidase (TvLiP): Genetic and Molecular Characterization
( Rita Terezinha De Oliveira Carneiro ),( Maiza Alves Lopes ),( Marilia Lordelo Cardoso Silva ),( Veronica Da Silva Santos ),( Volnei Brito De Souza ),( Aurizangela Oliveira De Sousa ),( Carlos Primin 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1
White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7<sup>th</sup> day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
Cobalt and Magnetite Functionalized Virus Nanofibers for Hydrogen Generation
Kwak, Eun-A.,Dutra, Dé,bora Volkart,Becker, Marilia Vier,Uh, Kyungchan,Jaworski, Justyn American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.3
<P>The use of virus based materials for nanotechnology applications has increased in recent years owing to their straightforward production, their ability to undergo surface modifications, and their inherently predictable structures. We show that the negatively charged surface of the native fd phage (virus) could facilitate ionic cross-linking via multivalent cobalt ions having opposite charge. Subsequent adsorption of magnetite to the cobalt/phage fibers allowed a high surface area network of cobalt and magnetite which was easily retrievable from solution using a simple magnet. With native fd phage as the template, we demonstrated the room temperature assembly of a cobalt/magnetite/phage nanofiber network. Formation of a catalytically active Co-B coating on the surface of the network structure was achieved by reduction with sodium borohydride. Examining the chemically reduced network, we found it may serve as a catalyst for controlled production of hydrogen gas from sodium borohydride. The embedded magnetite nanoparticles allowed for removal of the catalytic network, which can be of benefit for reactions requiring controlled timing of product formation such as for 'on demand' hydrogen generation.</P>
Grossi-De-Sa, Maria Fatima,De Magalhaes, Mariana Quezado,Silva, Marilia Santos,Silva, Shirley Margareth.Buffon,Dias, Simoni Campos,Nakasu, Erich Yukio Tempel,Brunetta, Patricia Sanglard Felipe,Oliveir Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Different isolates of the soil bacterium Bacillus thuringiensis produce multiple crystal (Cry) proteins toxic to a variety of insects, nematodes and protozoans. These insecticidal Cry toxins are known to be active against specific insect orders, being harmless to mammals, birds, amphibians, and reptiles. Due to these characteristics, genes encoding several Cry toxins have been engineered in order to be expressed by a variety of crop plants to control insectpests. The cotton boll weevil, Anthonomus grandis, and the fall armyworm, Spodoptera frugiperda, are the major economically devastating pests of cotton crop in Brazil, causing severe losses, mainly due to their endophytic habit, which results in damages to the cotton boll and floral bud structures. A cry1Ia-type gene, designated cry1Ia12, was isolated and cloned from the Bt S811 strain. Nucleotide sequencing of the cry1Ia12 gene revealed an open reading frame of 2160 bp, encoding a protein of 719 amino acid residues in length, with a predicted molecular mass of 81 kDa. The amino acid sequence of Cry1Ia12 is 99% identical to the known Cry1Ia proteins and differs from them only in one or two amino acid residues positioned along the three domains involved in the insecticidal activity of the toxin. The recombinant Cry1Ia12 protein, corresponding to the cry1Ia12 gene expressed in Escherichia coli cells, showed moderate toxicity towards first instar larvae of both cotton boll weevil and fall armyworm. The highest concentration of the recombinant Cry1Ia12 tested to achieve the maximum toxicities against cotton boll weevil larvae and fall armyworm larvae were 230 ${\mu}g/mL$ and 5 ${\mu}g/mL$, respectively. The herein demonstrated insecticidal activity of the recombinant Cry1Ia12 toxin against cotton boll weevil and fall armyworm larvae opens promising perspectives for the genetic engineering of cotton crop resistant to both these devastating pests in Brazil.
John Alexander Lata Guacho,Diogo Turiani Hourneaux De Moura,Igor Braga Ribeiro,Bruna Furia Buzetti Hourneaux de Moura,Megui Marilia Mansilla Gallegos,Thomas McCarty,Ricardo Katsuya Toma,Eduardo Guimar 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.2
Background/Aims: Carbon dioxide is increasingly used in insufflation during colonoscopy in adult patients; however, air insufflationremains the primary practice among pediatric gastroenterologists. This systematic review and meta-analysis aims to evaluateinsufflation using CO2 versus air in colonoscopies in pediatric patients. Methods: Individualized search strategies were performed using MEDLINE, Cochrane Library, EMBASE, and LILACS databasesfollowing Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and Cochrane workingmethodology. Randomized control trials (RCTs) were selected for the present meta-analysis. Pooled proportions were calculated foroutcomes including procedure time and abdominal pain immediately and 24 hours post-procedure. Results: The initial search yielded 644 records, of which five RCTs with a total of 358 patients (CO2: n=178 versus air: n=180) wereincluded in the final analysis. The procedure time was not different between the CO2 and air insufflation groups (mean difference,10.84; 95% confidence interval [CI], -2.55 to 24.22; p=0.11). Abdominal pain immediately post-procedure was significantly lowerin the CO2 group (risk difference [RD], -0.15; 95% CI; -0.26 to -0.03; p=0.01) while abdominal pain at 24 hours post-procedure wassimilar (RD, -0.05; 95% CI; -0.11 to 0.01; p=0.11). Conclusions: Based on this systematic review and meta-analysis of RCT data, CO2 insufflation reduced abdominal pain immediatelyfollowing the procedure, while pain was similar at 24 hours post-procedure. These results suggest that CO2 is a preferred insufflationtechnique when performing colonoscopy in pediatric patients.
IL-10 prevents aging-associated inflammation and insulin resistance in skeletal muscle
Dagdeviren, Sezin,Jung, Dae Young,Friedline, Randall H.,Noh, Hye Lim,Kim, Jong Hun,Patel, Payal R.,Tsitsilianos, Nicholas,Inashima, Kunikazu,Tran, Duy A.,Hu, Xiaodi,Loubato, Marilia M.,Craige, Siobhan The Federation of American Societies for Experimen 2017 The FASEB Journal Vol.31 No.2
<P>Altered energy balance and insulin resistance are important characteristics of aging. Skeletal muscle is a major site of glucose disposal, and the role of aging-associated inflammation in skeletal muscle insulin resistance remains unclear. To investigate, we examined glucose metabolism in 18-mo-old transgenic mice with muscle-specific overexpression of IL-10 (M-IL10) and in wild-type mice during hyperinsulinemic-euglycemic clamping. Despite similar fat mass and energy balance, M-IL10 mice were protected from aging-associated insulin resistance with significant increases in glucose infusion rates, whole-body glucose turnover, and skeletal muscle glucose uptake (similar to 60%; P < 0.05), as compared to age-matched WT mice. This protective effect was associated with decreased muscle inflammation, but no changes in adipose tissue inflammation in aging M-IL10 mice. These results demonstrate the importance of skeletal muscle inflammation in aging-mediated insulin resistance, and our findings further implicate a potential therapeutic role of anti-inflammatory cytokine in the treatment of aging-mediated insulin resistance.</P>