http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
FE analysis of RC pipes under three-edge-bearing test: Pocket and diameter influence
Marcela Novischi Kataoka,Jefferson Lins da Silva,Luciane Marcela Filizola de Oliveira,Mounir Khalil El Debs 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.20 No.4
This paper studies on the behavior of reinforced concrete (RC) pipes used in basic sanitation in the conduction of storm water and sanitary sewer. Pipes with 800 mm and 1200 mm in diameter were analyzed. The 800 mm pipes were built with simple reinforcement and the 1200 mm pipes with double reinforcement. For the two diameters of pipes the presence or absence of the pocket was evaluated, and the denomination of each one is spigot and pocket pipe (SPP) and ogee joint pipe (OJP), respectively. The 3D numerical models reproduce the three-edge-bearing test that provides information about the strength and stiffness of the reinforced concrete pipes. The validation of the computational models was carried out comparing the vertical and horizontal displacements on the springline and crown/invert and it was also evaluated the reinforcement strains and the crack pattern. As a main conclusion, the numerical models represented satisfactorily the behavior of the pipes and can be used in future studies in parametric analysis.
Nasal Immunization Using Chitosan Nanoparticles with Glycoprotein B of Murine Cytomegalovirus
( Marcela Slovakova ),( Sylva Janovska ),( Radek Sleha ),( Vera Radochova ),( Alexandra Hatala ),( Nikola Mannova ),( Radovan Metelka ),( Ludovit Pudelka ),( Pavel Bostik ) 한국미생물생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.3
The use of nanoparticles as a delivery system for a specific antigen could solve many limitations of mucosal vaccine applications, such as low immunogenicity, or antigen protection and stabilization. In this study, we tested the ability of nasally administered chitosan nanoparticles loaded with glycoprotein B of murine cytomegalovirus to induce an immune response in an animal model. The choice of chitosan nanoparticle type was made by in vitro evaluation of sorption efficiency and antigen release. Three types of chitosan nanoparticles were prepared: crosslinked with tripolyphosphate, coated with hyaluronic acid, and in complex with polycaprolactone. The hydrodynamic size of the nanoparticles by dynamic light scattering, zeta potential, Fourier transform infrared spectroscopy, scanning electron microscopy, stability, loading efficiency, and release kinetics with ovalbumin were evaluated. Balb/c mice were immunized intranasally using the three-dose protocol with nanoparticles, gB, and adjuvants Poly(I:C) and CpG ODN. Subsequently, the humoral and cell-mediated antigen-specific immune response was determined. On the basis of the properties of the tested nanoparticles, the cross-linked nanoparticles were considered optimal for further investigation. The results show that nanoparticles with Poly(I:C) and with gB alone raised IgG antibody levels above the negative control. In the case of mucosal IgA, only gB alone weakly induced the production of IgA antibodies compared to saline-immunized mice. The number of activated cells increased slightly in mice immunized with nanoparticles and gB compared to those immunized with gB alone or to negative control. The results demonstrated that chitosan nanoparticles could have potential in the development of mucosal vaccines.
Marcela Mireles,,M.A. Quevedo Lopez 한국물리학회 2016 Current Applied Physics Vol.16 No.11
Thin films of titanium/silicon (Ti:Si) are widely employed in the electronics industry because of their metal-like characteristics. The use of films as thin film resistor (TFR) based on Ti:Si has not yet been reported. In this paper, the TFR characteristics of TiSiON with different compositions and deposited in Argon atmosphere with either 1% Oxygen or 1% Oxygen/3% Nitrogen are studied. The film composition was varied through a co-sputtering approach from titanium and silicon targets and the sheet resistance (Rsh) and temperature coefficient of resistance (TCR) were evaluated. The film composition was evaluated by X-ray photoelectron spectroscopy (XPS); carrier mobility, type and concentration by Hall effect methods and film microstructure by X-Ray diffraction. Nitrogen addition during the deposition reduces oxidized species in the TFR and increases film stability. The addition of nitrogen results in TFR films with partially oxidized titanium and silicon and TFR values closer to zero TCR without impacting film resistance. Films deposited without nitrogen result in more unstable films and larger TCR. A near-zero TCR film was found for a Ti:Si ratio of 1.6, exhibiting a Rsh at 25 C of 0.7 kOhms with a TCR value of 171 ppm/C after annealing at 450 C in forming gas.
ESR1 Mutation Detection and Dynamics in Meningeal Carcinomatosis in Breast Cancer
Marcela Carausu,Samia Melaabi,Jean-Yves Pierga,François-Clément Bidard,Luc Cabel 한국유방암학회 2020 Journal of breast cancer Vol.23 No.2
ESR1 mutation is frequently encountered in hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer (MBC), especially after aromatase inhibitor (AI) therapy, as a mechanism of resistance to endocrine therapy. Circulating tumor DNA-based detection of ESR1 mutation in plasma has been demonstrated as a prognostic and predictive factor for poor outcomes in subsequent AI therapy. In this case report, for the first time, we describe the detection of ESR1 mutation (p.Tyr537Ser) only in the cerebrospinal fluid (CSF) and not in the plasma of a patient with isolated leptomeningeal progression who was treated with AI for HR-positive, HER2-negative MBC (bone metastasis only). Circulating tumor DNA levels also appeared to be correlated with clinical evolution. We suggest that in the presence of isolated leptomeningeal metastasis and when tamoxifen or AI has been prescribed for HR-positive MBC, CSF should be screened for ESR1 mutations to potentially adjust systemic treatment.
Marcela S ˇ afratova,Anna Hosˇt’a´lkova,Daniela Hulcova,Katerˇina Breiterova,Veronika Hrabcova,Marta Machado,Diana Fontinha,Miguel Prudeˆncio,Jirˇı´ Kunes,Jakub Chlebek,Daniel Jun,Martina Hrabinova,Lu 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.2
Fifteen Amaryllidaceae alkaloids (1–15) of variousstructural types were isolated by standard chromatographicmethods from fresh bulbs of Narcissus poeticus cv. Pink Parasol. The chemical structures were elucidated byMS, and 1D and 2D NMR spectroscopic analyses, and bycomparison with literature data. Narcipavline (5) and narcikachnine(6) are reported here for the first time. In theirstructure are combined two basic structural types ofAmaryllidaceae alkaloids (galanthamine- and galanthindole-structural types), which represent a new structuraltype of these compounds. Alkaloids isolated in sufficientamounts were evaluated for their human erythrocyticacetylcholinesterase, and human serum butyrylcholinesterase(HuBuChE) inhibition activity using Ellman’smethod. Z-Gly-Pro-p-nitroanilide was used assubstrate in the prolyl oligopeptidase (POP) assay. Untestedalkaloids were also screened for their cytotoxic activityagainst a small panel of human cancer cells, which spannedcell lines from different tissue types. In parallel, MRC-5human fibroblasts were employed to determine overalltoxicity against noncancerous cells. Some compounds wereevaluated for their antiprotozoal activity. The newly isolatedalkaloid narcipavline (5) showed interestingHuBuChE inhibition activity (IC50 = 24.4 ± 1.2 lM), andnorlycoramine (11) demonstrated promising POP inhibition(IC50 = 0.21 ± 0.01 mM).
Marcela S. Tsuboy,Juliana C. Marcarini,Rodrigo C. Luiz,Iuri B. Barros,Dalva T. Ferreira,Lu´cia R. Ribeiro,Ma´rio S. Mantovani 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3
Coccoloba mollis (Family Polygonaceae) is a medicinal plant popularly used in cases of memory loss, stress, insomnia, anemia, impaired vision, and sexual impotence, but the scientific literature, to date, lacks studies on the biological effects of this species, particularly with regard to cytotoxicity and induction of DNA damage. The aim of the present study was to assess in vitro (in hepatic HTC cells) ethanolic extracts of the roots and leaves of C. mollis for cytotoxicity, genotoxicity, and induction of apoptosis. For these evaluations the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay, comet assay, micronucleus test with cytokinesis block, and an in situ test for detection of apoptotic cells with acridine orange staining were used. The results showed that the extract obtained from the roots of C. mollis is more cytotoxic than that obtained from the leaves and that the reduction in cell viability observed in the MTT assay was a result, at least in part, from the induction of apoptosis. Both extracts induced DNA damage at a concentration of 20μg/mL in the comet assay, but no genotoxicity was detected with any of the treatments carried out in the micronucleus test.
In situ screening of 3-arylcoumarin derivatives reveals new inhibitors of mast cell degranulation
Marcela de Souza Santos,Maria Perpe´tua Freire de Morais Del Lama,Laila Aparecida Deliberto,Fla´vio da Silva Emery,Moˆnica Tallarico Pupo,Rose Mary Zumstein Georgetto Naal 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.6
Due to the severity and high prevalence ofallergic diseases, there is growing interest in the developmentof inhibitors of such conditions. 3-Arylcoumarinderivatives emerge as promising compounds for thetreatment of allergic disorders, in particular due to theirclose structural similarity to flavonoids, whose anti-allergicactivity has been extensively reported. The aim of this workwas to perform a screening of a set of 3-arylcoumarins aspotential inhibitors of mast cell degranulation, a key eventfor the development of allergic reactions. For that purpose, itwas utilized a biosensor model based on mast cells, whosein vitro assay allows for such screening, in a high throughputfashion, and also permits bringing to attention some coumarinstructural features that are important for their biologicalactivity. The mast cell-based biosensor was shown todiscriminate, with high sensitivity and reproducibility,between coumarins that did not affect or caused differentdegrees of inhibition of degranulation. Among activecoumarins, some substituents could be accounted for theirinhibitory activity, such as the hydroxylation of positions 6and 20 of 3-phenylcoumarins, in addition to catechol, aminoand thiophene moieties. In summary, 3-arylcoumarins couldbe suggested as potential candidates for the developmentof new anti-allergic drugs.
Marcela S. Tsuboy,Juliana C. Marcarini,Alecsandra O. de Souza,Nata´lia A. de Paula,Daniel J. Dorta,Ma´rio S. Mantovani,Lucia R. Ribeiro 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.2
Several studies have demonstrated that a balanced diet can contribute to better human health. For this reason, soy-based food and pure isoflavones (pills) are one of the most consumed. The association of this consumption and lower risks of chronic diseases and cancer is well established for the Asian population and has been attracting thw attention of people worldwide, especially women at menopause who seek to alleviate the symptoms associated with the lack of estrogen. Despite positive epidemiological data, concerns still exist because of conflicting results found in scientific literature with relation to the role of isoflavones in breast and hormone-related cancers. The aim of our study was to investigate the cytotoxicity, induction of apoptosis, and changes in apoptosis-related genes of maximal physiological serum levels of the isoflavone genistein (Gen) in MCF-7 tumoral cells and in HB4a non-tumoral cells. In addition, induction of cell cycle arrest was also investigated. Only supraphysiological levels of Gen (50 and 100 lM) were cytotoxic to these cell lines. Concentrations of 10 and 25 lM did not induce apoptosis and significant changes in expression of the studied genes. Positive results were found only in cell cycle analysis: G0/G1 delay of MCF-7 cells in both concentrations of Gen and at 25 lM in HB4a cells. It is the first study investigating effects of Gen in the HB4a cell line. Thus, despite the lack of apoptosis induction (generally found with high concentrations), Gen at physiologically relevant serum levels still exerts chemopreventive effects through the modulation of cell cycle.