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Youngsoo Kang,Jeonghoon Ko,Manwi Han,Yeonjae Bae,Yonggyun Kim 한국응용곤충학회 2005 Journal of Asia-Pacific Entomology Vol.8 No.3
The Oriental mayfly, Ephemera orientalisMcLachlan, 1875 (Ephemeroptera: Ephemeridae), can be a promising candidate used for monitoring environ-mental risk in aquatic ecosystem. Its quite large distri-its genetic variability due to local selection and adap-tation according to environmental hazard gradient. Before addressing the hypothesis, we needed to havepolymorphic loci to discriminate individuals and cha-racterize a specific population. For this reason, mito-chondrial cytochrome oxidase-I subunit (mtCO-I) was chosen because of its relatively high mutational rate among mitochondrial genome. Two regional E. ori-entalis populations were obtained at Andong andHoengseong, where few industrial complexes werelocated and could be regarded as being conserved in genetic variability. The amplified product showed550bp in all tested samples and digested with Alu I andRsa I. Alu I cuts one site in wild type (major), buttwo sites in variants. Rsa I cuts one site in both wild and variant samples, but difered in position of the restriction site. With these markers, Andong and Hoeng-seong populations recorded variant ratios of 1.7% and 30.0%, respectively. However, wide-regional sampleand Russia did not show any variant types in thisanalysis, probably due to quite small numbers of sam-ples (one or two) in each location. In highly poly-morphic Hoengseong population, thorax morphological characters reflected the restriction site polymorphism.
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