http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Mai-Ru (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
A Study of the Evolution Context of State Intervention in the Han Dynasty
Mai-Ru Zhao,이병채 한국무역연구원 2018 무역연구 Vol.14 No.6
There are three main factors affecting the evolution context of state intervention in the Han dynasty: the strength of national control, public spending, and the ideology of the ruler. The strength of state intervention under different situations are the features of evolution context. According to its effect and intensity, state intervention in the Han dynasty can be divided into four types: positive strong, positive weak, negative strong, and negative weak. These four types are changing alternatively during the whole Han dynasty period. Based on the dynamic evolution context of the Han dynasty, there are seven distinguishable stages of state intervention, which provide enlightenment for the contemporary time. Promoting the stable development of economy and national power should be the purpose of state intervention, promoting the non-zero-sum game of various stakeholders should be the focus of state intervention, forming a variety of ways of state intervention should be considered, and so on. In the new era, it is desirable to adjust the type, scope, and degree of state intervention with the help of the rules developed in the Han dynasty.
-
Ru-ren Li,Qun-li Yu,Ling Han,Liang-yan Rong,Meng-meng Yang,Mai-rui An 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.11
A novel hyaluronidase (BgHya1) from Yak testis was isolated and shown to have compara-tively high activity on sodium hyaluronate. However, surveys on BgHya1 are still limited. The enzyme was purifiedthrough gel filtration on Sephacryl S-100 and cation-exchange on SP Sepharose fast flow; the purity was confirmedby a reverse phase FPLC Shodex C4 column. The specific activity of the purified BgHya1 was 20.4 U/mg assayed bythe colorimetric method against 0.85 U/mg for the crude enzyme, representing a 24-fold purification. It was a monomericprotein of 55 kDa estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and SephacrylS-200. It exhibited maximum activity in the presence of 0.15 M NaCl at 37 oC, pH 3.8, and a specificity to sodiumhyaluronate higher than that of chondroitin-4-sulfate, chondroitin-6-sulfate, and dermatan. The Km value for BgHya1,using sodium hyaluronate as substrate, was 0.106 mg/mL. Activity of BgHya1 was inhibited mildly by Ca2+and Fe2+,and significantly by Fe3+, Mg2+, EDTA, urea, heparin, and 0.5 M NaCl. It was not affected by Cu2+,Zn2+,Co2+, ascorbicacid, PMSF, DTT, glutathione (reduced), or L-cysteine. BgHya1 was shown to be heat unstable in the range of 4-45 oC. In terms of storage stability, 92% of the activity was retained after four weeks at 4 oC, and 58% at room temperature. In addition, adding BSA (1.0 mg/mL) to the enzyme sample prior to freezing resulted in complete retention of enzymeactivity. This work yielded a high purity hyaluronidase, the first one isolated from by-product.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
