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Marcela S. Tsuboy,Juliana C. Marcarini,Rodrigo C. Luiz,Iuri B. Barros,Dalva T. Ferreira,Lu´cia R. Ribeiro,Ma´rio S. Mantovani 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3
Coccoloba mollis (Family Polygonaceae) is a medicinal plant popularly used in cases of memory loss, stress, insomnia, anemia, impaired vision, and sexual impotence, but the scientific literature, to date, lacks studies on the biological effects of this species, particularly with regard to cytotoxicity and induction of DNA damage. The aim of the present study was to assess in vitro (in hepatic HTC cells) ethanolic extracts of the roots and leaves of C. mollis for cytotoxicity, genotoxicity, and induction of apoptosis. For these evaluations the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay, comet assay, micronucleus test with cytokinesis block, and an in situ test for detection of apoptotic cells with acridine orange staining were used. The results showed that the extract obtained from the roots of C. mollis is more cytotoxic than that obtained from the leaves and that the reduction in cell viability observed in the MTT assay was a result, at least in part, from the induction of apoptosis. Both extracts induced DNA damage at a concentration of 20μg/mL in the comet assay, but no genotoxicity was detected with any of the treatments carried out in the micronucleus test.
Wen-Yue Liu,Xiaofang Zhang,Gang Li,Liang-Jie Tang,Pei-Wu Zhu,Rafael S. Rios,Kenneth I. Zheng,Hong-Lei Ma,Xiao-Dong Wang,Qiuwei Pan,Robert J. de Knegt,Luca Valenti,Mohsen Ghanbari,Ming-Hua Zheng 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.2
Background/Aims: Non-alcoholic fatty liver disease (NAFLD) is closely associated with metabolic dysfunction. Among the multiple factors, genetic variation acts as important modifiers. Klotho, an enzyme encoded by the klotho (KL) gene in human, has been implicated in the pathogenesis of metabolic dysfunctions. However, the impact of variants in KL on NAFLD risk remains poorly understood. The aim of this study was to investigate the impact of KL rs495392 C>A polymorphism on the histological severity of NAFLD. Methods: We evaluated the impact of the KL rs495392 polymorphism on liver histology in 531 Chinese with NAFLD and replicated that in the population-based Rotterdam Study cohort. The interactions between the rs495392, vitamin D, and patatin-like phospholipase domain containing 3 (PNPLA3) rs738409 polymorphism were also analyzed. Results: Carriage of the rs495392 A allele had a protective effect on steatosis severity (odds ratio [OR], 0.61; 95% confidence interval [CI], 0.42–0.89; P=0.010) in Chinese patients. After adjustment for potential confounders, the A allele remained significant with a protective effect (OR, 0.66; 95% CI, 0.45–0.98; P=0.040). The effect on hepatic steatosis was confirmed in the Rotterdam Study cohort. Additional analysis showed the association between serum vitamin D levels and NAFLD specifically in rs495392 A allele carriers, but not in non-carriers. Moreover, we found that the rs495392 A allele attenuated the detrimental impact of PNPLA3 rs738409 G allele on the risk of severe hepatic steatosis. Conclusions: The KL rs495392 polymorphism has a protective effect against hepatic steatosis in patients with NAFLD.
Marcela S. Tsuboy,Juliana C. Marcarini,Alecsandra O. de Souza,Nata´lia A. de Paula,Daniel J. Dorta,Ma´rio S. Mantovani,Lucia R. Ribeiro 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.2
Several studies have demonstrated that a balanced diet can contribute to better human health. For this reason, soy-based food and pure isoflavones (pills) are one of the most consumed. The association of this consumption and lower risks of chronic diseases and cancer is well established for the Asian population and has been attracting thw attention of people worldwide, especially women at menopause who seek to alleviate the symptoms associated with the lack of estrogen. Despite positive epidemiological data, concerns still exist because of conflicting results found in scientific literature with relation to the role of isoflavones in breast and hormone-related cancers. The aim of our study was to investigate the cytotoxicity, induction of apoptosis, and changes in apoptosis-related genes of maximal physiological serum levels of the isoflavone genistein (Gen) in MCF-7 tumoral cells and in HB4a non-tumoral cells. In addition, induction of cell cycle arrest was also investigated. Only supraphysiological levels of Gen (50 and 100 lM) were cytotoxic to these cell lines. Concentrations of 10 and 25 lM did not induce apoptosis and significant changes in expression of the studied genes. Positive results were found only in cell cycle analysis: G0/G1 delay of MCF-7 cells in both concentrations of Gen and at 25 lM in HB4a cells. It is the first study investigating effects of Gen in the HB4a cell line. Thus, despite the lack of apoptosis induction (generally found with high concentrations), Gen at physiologically relevant serum levels still exerts chemopreventive effects through the modulation of cell cycle.
Eveline Kelle Ursulino Pontes,Hider Machado Melo,Jose´ Walter Arau´ jo Nogueira,Nairley Cardoso Sa´ Firmino,Ma´rio Geraldo de Carvalho,Francisco Eduardo Araga˜o Catunda Ju´nior,Theodora Thays Arruda C 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
Medicinal plants with antimicrobial action havebeen investigated for uses against biofilms, among which,Cymbopogon nardus, citronella, stands out as a promisingspecies. The present study aims to evaluate the antimicrobialand antibiofilm action of the essential oil of C. nardus (EOCN) and geraniol on Gram-negative and positivebacteria from the determination of minimum inhibitoryconcentration (MIC) and minimum bactericidal concentrationand inhibition of biofilms. In the results, the EOCNproduced a 41 mm halo on S. aureus, which was susceptiblewith MIC values of 0.5 and 0.25 mg/mL for theEOCN and geraniol respectively, both with bactericidaleffect. The antibiofilm action was confirmed, the EOCNand geraniol reduced the biofilm biomass of S. aureus up to100% between 0.5 and 4 mg/mL concentrations. Thereduction of cell viability was 0.25 and 1 mg/mL, ofEOCN and geraniol, respectively. EOCN and geraniol wereshown to be promising antibiotic against S. aureus.