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Cone-beam computed tomography findings of impacted upper canines
Ludmilla Mota da Silva Santos,Luana Costa Bastos,Christiano Oliveira-Santos,Silvio José Albergaria da Silva,Frederico Sampaio Neves,Paulo Sérgio Flores Campos 대한영상치의학회 2014 Imaging Science in Dentistry Vol.44 No.4
Purpose: To describe the features of impacted upper canines and their relationship with adjacent structures through three-dimensional cone-beam computed tomography (CBCT) images. Materials and Methods: Using the CBCT scans of 79 upper impacted canines, we evaluated the following parameters: gender, unilateral/bilateral occurrence, location, presence and degree of root resorption of adjacent teeth (mild, moderate, or severe), root dilaceration, dental follicle width, and presence of other associated local conditions. Results: Most of the impacted canines were observed in females (56 cases), unilaterally (51 cases), and at a palatine location (53 cases). Root resorption in adjacent teeth and root dilaceration were observed in 55 and 47 impacted canines, respectively. In most of the cases, the width of the dental follicle of the canine was normal; it was abnormally wide in 20 cases. A statistically significant association was observed for all variables, except for root dilaceration (p=0.115) and the side of impaction (p=0.260). Conclusion: Root resorption of adjacent teeth was present in most cases of canine impaction, mostly affecting adjacent lateral incisors to a mild degree. A wide dental follicle of impacted canines was not associated with a higher incidence of external root resorption of adjacent teeth.
Cone-beam computed tomography findings of impacted upper canines
Da Silva Santos, Ludmilla Mota,Bastos, Luana Costa,Oliveira-Santos, Christiano,Da Silva, Silvio Jose Albergaria,Neves, Frederico Sampaio,Campos, Paulo Sergio Flores Korean Academy of Oral and Maxillofacial Radiology 2014 Imaging Science in Dentistry Vol.44 No.4
Purpose: To describe the features of impacted upper canines and their relationship with adjacent structures through three-dimensional cone-beam computed tomography (CBCT) images. Materials and Methods: Using the CBCT scans of 79 upper impacted canines, we evaluated the following parameters: gender, unilateral/bilateral occurrence, location, presence and degree of root resorption of adjacent teeth (mild, moderate, or severe), root dilaceration, dental follicle width, and presence of other associated local conditions. Results: Most of the impacted canines were observed in females (56 cases), unilaterally (51 cases), and at a palatine location (53 cases). Root resorption in adjacent teeth and root dilaceration were observed in 55 and 47 impacted canines, respectively. In most of the cases, the width of the dental follicle of the canine was normal; it was abnormally wide in 20 cases. A statistically significant association was observed for all variables, except for root dilaceration (p=0.115) and the side of impaction (p=0.260). Conclusion: Root resorption of adjacent teeth was present in most cases of canine impaction, mostly affecting adjacent lateral incisors to a mild degree. A wide dental follicle of impacted canines was not associated with a higher incidence of external root resorption of adjacent teeth.
SEED DEVELOPMENT : DEVELOPMENTAL AND METABOLIC REGULATION OF STORAGE COMPOUND SYNTHESIS
Ulrich Wobus,Hans Weber,Ludmilla Borisjuk,Reinhard Panitz,Ute Heim,Peter Buchner,Helmut Bäumlein,Winfriede Weschke Plant molecular biology and biotechnology research 1994 Proceedings the 2nd Korean-Germany joint symposium Vol.1994 No.-
Development of a novel peptide microarray for large-scale epitope mapping of food allergens
Lin, Jing,Bardina, Ludmilla,Shreffler, Wayne G.,Andreae, Doerthe A.,Ge, Yongchao,Wang, Julie,Bruni, Francesca M.,Fu, Zhiyan,Han, Youngshin,Sampson, Hugh A. Elsevier 2009 The Journal of allergy and clinical immunology Vol.124 No.2
<P><B>Background</B></P><P>The peptide microarray is a novel assay that facilitates high-throughput screening of peptides with a small quantity of sample.</P><P><B>Objective</B></P><P>We sought to use overlapping peptides of milk allergenic proteins as a model system to establish a reliable and sensitive peptide microarray-based immunoassay for large-scale epitope mapping of food allergens.</P><P><B>Methods</B></P><P>A milk peptide microarray was developed by using commercially synthesized peptides (20-mers, 3 offset) covering the primary sequences of α<SUB>s1</SUB>-casein, α<SUB>s2</SUB>-casein, β-casein, κ-casein, and β-lactoglobulin. Conditions for printing and immunolabeling were optimized using a serum pool of 5 patients with milk allergy. Reproducibility of the milk peptide microarray was evaluated using replicate arrays immunolabeled with the serum pool, whereas specificity and sensitivity were assessed by using serial dilution of the serum pool and a peptide inhibition assay.</P><P><B>Results</B></P><P>Our results show that epitopes identified by the peptide microarray were mostly consistent with those identified previously by SPOT membrane technology, but with specific binding to a few newly identified epitopes of milk allergens. Data from replicate arrays were reproducible (<I>r</I> ≥ 0.92) regardless of printing lots, immunolabeling, and serum pool batches. Using the serially diluted serum pool, we confirmed that IgE antibody binding detected in the array was specific. Peptide inhibition of IgE binding to the same peptide and overlapping peptides further confirmed the specificity of the array.</P><P><B>Conclusion</B></P><P>A reliable peptide microarray was established for large-scale IgE epitope mapping of milk allergens, and this robust technology could be applied for epitope mapping of other food allergens.</P>
Seo, Ho Seong,Minasov, George,Seepersaud, Ravin,Doran, Kelly S.,Dubrovska, Ievgeniia,Shuvalova, Ludmilla,Anderson, Wayne F.,Iverson, Tina M.,Sullam, Paul M. American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.50
<▼1><P><B>Background:</B> The serine-rich repeat glycoproteins Srr1 and Srr2 are surface adhesins of <I>Streptococcus agalactiae</I> important for pathogenicity.</P><P><B>Results:</B> Both Srrs bind tandem repeats of the fibrinogen Aα chain, but Srr2 has greater affinity explained by structure-function analysis of the Srrs.</P><P><B>Conclusion:</B> A dock, lock, and latch mechanism describes the Srr-fibrinogen interaction.</P><P><B>Significance:</B> The higher affinity of Srr2 may contribute to the hypervirulence of Srr2-expressing strains.</P></▼1><▼2><P>The serine-rich repeat glycoproteins of Gram-positive bacteria comprise a large family of cell wall proteins. <I>Streptococcus agalactiae</I> (group B <I>streptococcus</I>, GBS) expresses either Srr1 or Srr2 on its surface, depending on the strain. Srr1 has recently been shown to bind fibrinogen, and this interaction contributes to the pathogenesis of GBS meningitis. Although strains expressing Srr2 appear to be hypervirulent, no ligand for this adhesin has been described. We now demonstrate that Srr2 also binds human fibrinogen and that this interaction promotes GBS attachment to endothelial cells. Recombinant Srr1 and Srr2 bound fibrinogen <I>in vitro</I>, with affinities of <I>K<SUB>D</SUB></I> = 2.1 × 10<SUP>−5</SUP> and 3.7 × 10<SUP>−6</SUP><SMALL>M</SMALL>, respectively, as measured by surface plasmon resonance spectroscopy. The binding site for Srr1 and Srr2 was localized to tandem repeats 6–8 of the fibrinogen Aα chain. The structures of both the Srr1 and Srr2 binding regions were determined and, in combination with mutagenesis studies, suggest that both Srr1 and Srr2 interact with a segment of these repeats via a “dock, lock, and latch” mechanism. Moreover, properties of the latch region may account for the increased affinity between Srr2 and fibrinogen. Together, these studies identify how greater affinity of Srr2 for fibrinogen may contribute to the increased virulence associated with Srr2-expressing strains.</P></▼2>