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Surgical Intervention to Rescue Hirschsprung Disease in a Rat Model
( Lincon A Stamp ),( Florian Obermayr ),( Louise Pontell ),( Heather M Young ),( Dan Xie ),( David H Croaker ),( Zan-min Song ),( John B Furness ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.4
Background/Aims Rats with a spontaneous null mutation in endothelin receptor type B or Ednrb (sl/sl; spotting lethal) lack enteric neurons in the distal bowel and usually die within the first week after birth. This early postnatal lethality limits their use for examining the potential of cell therapy to treat Hirschsprung disease, and for studies of the influence of EDNRB on the mature CNS and vascular systems. Methods We have developed a surgical intervention to prolong the life of the spotting lethal sl/sl rat, in which we perform a colostomy on postnatal (P) day 4-6 rats to avoid the fatal obstruction caused by the lack of colonic enteric neurons. Results The stomas remained patent and functional and the rats matured normally following surgery. Weight gains were comparable between control and Hirschsprung phenotype (sl/sl) rats, which were followed until 4 weeks after surgery (5 weeks old). We confirmed the absence of enteric neurons in the distal colon of rats whose lives were saved by the surgical intervention. Conclusions This study provides a novel approach for studying EDNRB signalling in multiple organ systems in mature rats, including an animal model to study the efficacy of cell therapy to treat Hirschsprung disease. (J Neurogastroenterol Motil 2015;21:552-559)
Reaction of Cauliflower Genotypes to Black Rot of Crucifers
Lincon Rafael da Silva,Renan César Dias da Silva,Atalita Francis Cardoso,Gláucia de Mello Pelá,Daniel Diego Costa Carvalho 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.2
This study aimed to evaluate six cauliflower genotypes regarding their resistance to black rot and their production performance. To do so, it was conducted two field experiments in Ipameri, Goiás, Brazil, in 2012 and 2013. It was used a randomized block design, with four replications (total of 24 plots). Each plot consisted of three planting lines 2.5 m long (six plants/line), spaced 1.0 m apart, for a total area of 7.5 m2. Evaluations of black rot severity were performed at 45 days after transplanting, this is, 75 days after sowing (DAS), and yield evaluations at 90 to 105 DAS. The Verona 184 genotype was the most resistant to black rot, showing 1.87 and 2.25% of leaf area covered by black rot symptom (LACBRS) in 2012 and 2013. However, it was not among the most productive materials. The yield of the genotypes varied between 15.14 and 25.83 t/ha in both years, Lisvera F1 (21.78 and 24.60 t/ha) and Cindy (19.95 and 23.56 t/ha) being the most productive. However, Lisvera F1 showed 6.37 and 9.37% of LACBRS and Cindy showed 14.25 and 14.87% of LACBRS in 2012 and 2013, being both considered as tolerant to black rot.
Lincon Rafael da Silva,Leonardo Luís de Barros Rodrigues,Amanda Silva Botelho,Bruna Sartório de Castro,Paulo Henrique Pereira Costa Muniz,Maria Carolina Blassioli Moraes,Sueli Corrêa Marques de Mello 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.1
Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.
Mazumder, Lincon,Hasan, Mehedi,Rus’d, Ahmed Abu,Islam, Mohammad Ariful Korea Genome Organization 2021 Genomics & informatics Vol.19 No.4
Campylobacter jejuni is one of the most prevalent organisms associated with foodborne illness across the globe causing campylobacteriosis and gastritis. Many proteins of C. jejuni are still unidentified. The purpose of this study was to determine the structure and function of a non-annotated hypothetical protein (HP) from C. jejuni. A number of properties like physiochemical characteristics, 3D structure, and functional annotation of the HP (accession No. CAG2129885.1) were predicted using various bioinformatics tools followed by further validation and quality assessment. Moreover, the protein-protein interactions and active site were obtained from the STRING and CASTp server, respectively. The hypothesized protein possesses various characteristics including an acidic pH, thermal stability, water solubility, and cytoplasmic distribution. While alpha-helix and random coil structures are the most prominent structural components of this protein, most of it is formed of helices and coils. Along with expected quality, the 3D model has been found to be novel. This study has identified the potential role of the HP in 2-methylcitric acid cycle and propionate catabolism. Furthermore, protein-protein interactions revealed several significant functional partners. The in-silico characterization of this protein will assist to understand its molecular mechanism of action better. The methodology of this study would also serve as the basis for additional research into proteomic and genomic data for functional potential identification.
Reaction of Cauliflower Genotypes to Black Rot of Crucifers
da Silva, Lincon Rafael,da Silva, Renan Cesar Dias,Cardoso, Atalita Francis,de Mello Pela, Glaucia,Carvalho, Daniel Diego Costa The Korean Society of Plant Pathology 2015 Plant Pathology Journal Vol.31 No.2
This study aimed to evaluate six cauliflower genotypes regarding their resistance to black rot and their production performance. To do so, it was conducted two field experiments in Ipameri, $Goi\acute{a}s$, Brazil, in 2012 and 2013. It was used a randomized block design, with four replications (total of 24 plots). Each plot consisted of three planting lines 2.5 m long (six plants/line), spaced 1.0 m apart, for a total area of $7.5m^2$. Evaluations of black rot severity were performed at 45 days after transplanting, this is, 75 days after sowing (DAS), and yield evaluations at 90 to 105 DAS. The Verona 184 genotype was the most resistant to black rot, showing 1.87 and 2.25% of leaf area covered by black rot symptom (LACBRS) in 2012 and 2013. However, it was not among the most productive materials. The yield of the genotypes varied between 15.14 and 25.83 t/ha in both years, Lisvera F1 (21.78 and 24.60 t/ha) and Cindy (19.95 and 23.56 t/ha) being the most productive. However, Lisvera F1 showed 6.37 and 9.37% of LACBRS and Cindy showed 14.25 and 14.87% of LACBRS in 2012 and 2013, being both considered as tolerant to black rot.
Guilherme Dognani,Flávio Camargo Cabrera,Aldo Eloizo Job,Deuber Lincon da Silva Agostini 한국섬유공학회 2019 Fibers and polymers Vol.20 No.3
In this paper, poly(vinylidene fluoride-co-hexafluoropropylene) nanofibers membranes were fabricated by facilesingle-capillary electrospinning at different times of production. The materials were characterized by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle (CA), mercury porosity intrusion (MIP), Tensile tests, capillary flow porosity (CFP) and, BET surface by nitrogen adsorption-desorption. The membranes were fabricated varying the fibers density, the thickness and the morphology. The samples presented a good fiber diameter distribution and an average diameter of 321 nm. AFM results showed high porosity (82.7-87.5 %), a high contact angle of 159.2 o, mean pore size of 0.637 μm and hydrophobicity of the membranes. A low value of permeate pure water flux, 5.2 %, was observed for the densest fabricated membrane. The porosity of fibers diameters was similar for all samples, which is an important parameter as they can be potential materials for membrane distillation process.