Long Non-Coding RNA CCAT1 Acts as a Competing Endogenous RNA to Regulate Cell Growth and Differentiation in Acute Myeloid Leukemia

Lianxiang Chen,Wei Wang,Lixia Cao,Zhijun Li,Xing Wang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.4

Long non-coding RNAs (lncRNAs) are involved in multi-ple cellular events, as well as in tumorigenesis. Colon cancer-associated transcript-1 (CCAT1) gene encodes an lncRNA whose over-activation was observed in an expanding list of primary human solid tumors and tumor cell lines, however its biological roles in acute myeloid leukaemia (AML) has not been reported yet at present. In this study, the aberrant upregulation of CCAT1 was detected in French-American-British M4 and M5 subtypes of adult AML patients. By gain- and loss-of-function analysis, we determined that CCAT1 repressed monocytic differentiation and promoted cell growth of HL-60 by sequestering tumor suppressive miR-155. Accordingly, a significant decrease in miR-155 level was detected in AML patients. Re-introduction of miR-155 into HL-60 cells restored monocytic maturation and repressed cell proliferation. Furthermore, CCAT1 could up-regulated c-Myc via its competing endogenous RNA (ceRNA) activity on miR-155. In conclusion, these results revealed new mechanism of lncRNA CCAT1 in AML development, and suggested that the manipulation of CCAT1 expression could serve as a potential strategy in AML therapy.

Long Non-Coding RNA CCAT1 Acts as a Competing Endogenous RNA to Regulate Cell Growth and Differentiation in Acute Myeloid Leukemia

Chen, Lianxiang,Wang, Wei,Cao, Lixia,Li, Zhijun,Wang, Xing Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.4

Long non-coding RNAs (lncRNAs) are involved in multiple cellular events, as well as in tumorigenesis. Colon cance-rassociated transcript-1 (CCAT1) gene encodes an lncRNA whose over-activation was observed in an expanding list of primary human solid tumors and tumor cell lines, however its biological roles in acute myeloid leukaemia (AML) has not been reported yet at present. In this study, the aberrant upregulation of CCAT1 was detected in French-American-British M4 and M5 subtypes of adult AML patients. By gain- and loss-of-function analysis, we determined that CCAT1 repressed monocytic differentiation and promoted cell growth of HL-60 by sequestering tumor suppressive miR-155. Accordingly, a significant decrease in miR-155 level was detected in AML patients. Reintroduction of miR-155 into HL-60 cells restored monocytic maturation and repressed cell proliferation. Furthermore, CCAT1 could up-regulated c-Myc via its competing endogenous RNA (ceRNA) activity on miR-155. In conclusion, these results revealed new mechanism of lncRNA CCAT1 in AML development, and suggested that the manipulation of CCAT1 expression could serve as a potential strategy in AML therapy.

Effects of low-dose organic trace minerals on performance, mineral status, and fecal mineral excretion of sows

Ma, Lianxiang,He, Junna,Lu, Xintao,Qiu, Jialing,Hou, Chuanchuan,Liu, Bing,Lin, Gang,Yu, Dongyou Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: To investigate the effects of low-dose trace mineral proteinates on reproductive performance, mineral status, milk immunoglobulin contents and fecal mineral excretion of sows. Methods: Eighty crossbred sows (Landrace×Large White) were randomly allocated to two groups in a 135-day trail, from breeding through 21 d postpartum. The two treatments were inorganic trace minerals (ITM): a basal diet+inorganic iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn) at 90, 15, 25 and 90 mg/kg, respectively and organic trace minerals (OTM): a basal diet+proteinates of Fe, Cu, Mn, and Zn at 72, 12, 20, and 72 mg/kg, respectively. Results: Compared with ITM, OTM significantly increased (p<0.05) the number of piglets with birthweight >1 kg, the litter weaning weight, and milk Fe, Cu contents. No significant differences (p>0.05) were observed on sow hair mineral contents or immunoglobulin M (IgM), IgG, and IgA contents in colostrum and milk. In comparsion to ITM, OTM decreased fecal Fe, Cu, Mn, and Zn contents of gestating sows (p<0.01) and Fe, Mn, and Zn in lactating sows (p<0.01). Conclusion: These results indicate that low-dose mineral proteinates can increase the number of piglets with birthweight >1 kg, the litter weaning weight and certain milk mineral concentrations while reducing fecal mineral excretion.

Study on the Establishment of Free Trade Zone Agricultural Tariffs in China, Japan and South Korea Based on the GTAP Model

Kou Zheng,Kou Lianxiang,Yang Honghong 한중경제문화학회 2019 한중경제문화연구 Vol.12 No.-

Effects of compound organic acid calcium on growth performance, hepatic antioxidation and intestinal barrier of male broilers under heat stress

He Junna,Ma Lianxiang,Qiu Jialing,Lu Xintao,Hou Chuanchuan,Liu Bing,Yu Dongyou 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.7

Objective: The aim of this study was to evaluate the effects of compound organic acid calcium (COAC) on growth performance, hepatic antioxidant status and intestinal barrier of male broilers under high ambient temperature (32.7°C). Methods: Nine hundred healthy one-d-old Cobb-500 male broiler chicks were randomly assigned into three groups with six replicates of 50 birds each. A basal diet supplemented with 0% (control), 0.4% and 0.8% COAC, respectively were fed to birds for 6 weeks. All treatments were under high ambient indoor temperature of 32.7°C, and had a constant calcium and available phosphorus ratio. Results: The results showed that, compared with control, the average daily gain of broilers in 0.4% and 0.8% was significantly increased and the ratio of feed to gain in in 0.4% and 0.8% was significantly decreased at 1 to 21, 22 to 42 and 1 to 42 days of age (p<0.05). Compared with control, 0.8% COAC slightly decreased (p = 0.093) the content of malondialdehyde in liver at 42 days of age while 0.4% COAC significantly decreased (p<0.05) the activity of alkaline phosphatase. Furthermore, 0.4% COAC significantly enhanced the intestinal barrier function via increasing jejunal and ileal ocln transcription, promoting jejunal mucin 2 transcription at 42 days of age (p<0.05), and decreasing jejunal toll-like receptor 2 (TLR-2) and ileal TLR-15, inducible nitric oxide synthase compared with control group (p<0.05). Whereas, no significant differences on the transcription of interleukin-1β in jejunum and ileum were observed among three treatments (p>0.05). Overall, heat stress caused by high natural environment temperature may induce the damage to hepatic antioxidation and intestinal barrier. Conclusion: Dietary inclusion of COAC can improve the tolerance of broilers to thermal environment through the modification of antioxidative parameters in liver and the mRNA expression of genes in intestinal barrier, resulting in an optimal inclusion level of 0.4%.

Correcting the Elastic-modulus Error of Quartz Glass Using Digital Speckle-pattern Interferometry

Ziyang Song,Weixian Li,Sijin Wu,Lianxiang Yang 한국광학회 2023 Current Optics and Photonics Vol.7 No.4

Three-point bending is the main method for measuring the elastic modulus of a thin plate. Although various displacement transducers may be used to measure the bending, these are single-point measurements, and it is difficult to eliminate the error caused by eccentric load and shear force. Error-correction models for the elastic modulus of quartz glass using digital speckle interferometry are proposed for eccentric load and shear force. First, the positional misalignment between maximum deflection and load is analyzed, and the error caused by eccentric load is corrected. Then, the additional displacement caused by shear force at different positions of the quartz glass plate is explored. The effect of shear deformation is also corrected, by measuring two points. Since digital speckle interferometry has the advantage of full-field measurement, it can simultaneously obtain deflection data for multiple points to realize error correction. Experimental results are presented to demonstrate that the proposed model can effectively correct the measurement error of the elastic modulus.

Low-dose of organic trace minerals reduced fecal mineral excretion without compromising performance of laying hens

Qiu, Jialing,Lu, Xintao,Ma, Lianxiang,Hou, Chuanchuan,He, Junna,Liu, Bing,Yu, Dongyou,Lin, Gang,Xu, Jiming Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: The objective of this study was to investigate the effects of low doses of organic trace minerals (iron, copper, manganese, and zinc) on productive performance, egg quality, yolk and tissue mineral retention, and fecal mineral excretion of laying hens during the late laying period. Methods: A total of 405 healthy hens (HY-Line White, 50-week-old) were randomly divided into 3 treatments, with 9 replicates per treatment and 15 birds per replicate. The dietary treatments included feeding a basal diet + inorganic trace minerals at commercial levels (CON), a basal diet + inorganic trace minerals at 1/3 commercial levels (ITM), and a basal diet + proteinated trace minerals at 1/3 commercial levels (TRT). The trial lasted for 56 days. Results: Compared to CON, ITM decreased (p<0.05) egg production, daily egg mass, albumen height, eggshell strength, yolk Fe concentration, serum alkaline phosphatase activity and total protein, and increased (p<0.05) egg loss and feed to egg ratio. Whereas with productive performance, egg quality, yolk mineral retention, and serum indices there were no differences (p>0.05) between CON and TRT. The concentrations of Fe and Mn in the tissue and tibia were changed notably in ITM relative to CON and TRT. Both ITM and TRT reduced (p<0.05) fecal mineral excretion compared to CON. Conclusion: These results indicate that dietary supplementation of low-dose organic trace minerals reduced fecal mineral excretion without negatively impacting hen performance and egg quality.

Magnetic properties of Bi-doped Y3Fe5O12 nanoparticles

Haitao Xu,Hua Yang,Wei Xu,Lianxiang Yu 한국물리학회 2008 Current Applied Physics Vol.8 No.1

Bi³+ substituted garnet nanoparticles Y₃. xBiχFe5O₁₂ (x = 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.3) were fabricated by a solgel methodand their crystalline structures and magnetic properties were investigated by using X-ray diraction (XRD), IR spectroscopy, thermalgravity analysisdierential thermal analysis (TG-DTA), transmission electron microscope (TEM), Mo¨ssbauer spectroscopy and vibrat-3. xBixFe5O12 have only peaks of the garnet structure.

Effect of Nd ion on the magnetic properties of Ni-Mn ferrite nanocrystal

Lijun Zhao,Wei Xu,Hua Yang,Lianxiang Yu 한국물리학회 2008 Current Applied Physics Vol.8 No.1

The nanocrystalline Ni0.7Mn 0.3Nd 0.1Fe1.9O4 ferrites were prepared by the emulsion method. X-ray diractometer (XRD), transmis-sion electron microscope (TEM), Mo¨ssbauer spectra and vibrating samples magnetometer (VSM) were used to study the structure, mor-phology and magnetic properties. The magnetic properties of Nd3+-doped Ni0.7Mn 0.3Fe2O4 nanocrystal ferrites were investigated indetail. The Ni0.7Mn 0.3Nd 0.1Fe1.9O4 nanocrystal ferrite with particle size of 10.7 nm shows superparamagnetism.

The Antiobesity Effects and Potential Mechanisms of Theaflavins

Jinghua Peng,Jing Hu,Yi Fang,Jun Wang,Yu Cao,Wenrui Liu,Lianxiang Duan 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.1

Theaflavins are the characteristic polyphenols in black tea which can be enzymatically synthesized. In thisreview, the effects and molecular mechanisms of theaflavins on obesity and its comorbidities, including dyslipidemia, insulinresistance, hepatic steatosis, and atherosclerosis, were summarized. Theaflavins ameliorate obesity potentially via reducingfood intake, inhibiting pancreatic lipase to reduce lipid absorption, activating the adenosine monophosphate-activated proteinkinase (AMPK), and regulating the gut microbiota. As to the comorbidities, theaflavins ameliorate hypercholesterolemia byinhibiting micelle formation to reduce cholesterol absorption. Theaflavins improve insulin sensitivity by increasing thesignaling of protein kinase B, eliminating glucose toxicity, and inhibiting inflammation. Theaflavins ameliorate hepaticsteatosis via activating AMPK. Theaflavins reduce atherosclerosis by upregulating nuclear factor erythropoietin-2-relatedfactor 2 signaling and inhibiting plasminogen activator inhibitor 1. In randomized controlled trails, black tea extractscontaining theaflavins reduced body weight in overweight people and improved glucose tolerance in healthy adults. Theamelioration on the hyperlipidemia and the prevention of coronary artery disease by black tea extracts were supportedby meta-analysis.