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( A Leum Kim ),( Son Young Park ),( Chi Ho Lee ),( Chung Hak Lee ),( Jung Kee Lee ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11
Bacteria recognize changes in their population density by sensing the concentration of signal molecules, N-acyl-homoserine lactones (AHLs). AHL-mediated quorum sensing (QS) plays a key role in biofilm formation, so the interference of QS, referred to as quorum quenching (QQ), has received a great deal of attention. A QQ strategy can be applied to membrane bioreactors (MBRs) for advanced wastewater treatment to control biofouling. To isolate QQ bacteria that can inhibit biofilm formation, we isolated diverse AHL-degrading bacteria from a laboratory-scale MBR and sludge from real wastewater treatment plants. A total of 225 AHLdegrading bacteria were isolated from the sludge sample by enrichment culture. Afipia sp., Acinetobacter sp. and Streptococcus sp. strains produced the intracellular QQ enzyme, whereas Pseudomonas sp., Micrococcus sp. and Staphylococcus sp. produced the extracellular QQ enzyme. In case of Microbacterium sp. and Rhodococcus sp., AHL-degrading activities were detected in the whole-cell assay and Rhodococcus sp. showed AHL-degrading activity in cell-free lysate as well. There has been no report for AHL-degrading capability in the case of Streptococcus sp. and Afipia sp. strains. Finally, inhibition of biofilm formation by isolated QQ bacteria or enzymes was observed on glass slides and 96-well microtiter plates using crystal violet staining. QQ strains or enzymes not only inhibited initial biofilm development but also reduced established biofilms.
( Ah Leum Lim ),( Cheol Hong Kim ),( Yong Il Hwang ),( Chang Youl Lee ),( Jeong Hee Choi ),( Taerim Shin ),( Yong Bum Park ),( Seung Hun Jang ),( Sang Myeon Park ),( Dong Gyu Kim ),( Myung Goo Lee ),( 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.5
Background: Chest tube drainage (CTD) is an indication for the treatment of pneumothorax, hemothroax and is used after a thoracic surgery. But, in the case of incomplete lung expansion, and/or persistent air leak from CTD, medical or surgical thoracoscopy or, if that is unavailable, limited thoracotomy, should be considered. We evaluate the efficacy of bronchoscopic injection of ethanolamine to control the persistent air leak in patients with CTD. Methods: Patients who had persistent or prolonged air leak from CTD were included, consecutively. We directly injected 1.0 mL solution of 5% ethanolamine oleate into a subsegmental or its distal bronchus, where it is a probable air leakage site, 1 to 21 times using an injection needle through a fiberoptic bronchoscope. Results: A total of 15 patients were enrolled; 14 cases of spontaneous pneumothorax [idiopathic 9, chronic obstructive pulmonary disease (COPD) 3, post-tuberculosis 2] and one case of empyema associated with broncho-pleural fistula. Of these, five were patients with persistent air leak from CTD, just after a surgical therapy, wedge resection with plication for blebs or bullae. With an ethanolamine injection therapy, 12 were successful but three (idiopathic, COPD and post-tuberculosis) failed, and were followed by a surgery (2 cases) or pleurodesis (1 case). Some adverse reactions, such as fever, chest pain and increased radiographic opacities occurred transiently, but resolved without any further events. With success, the time from the procedure to discharge was about 3 days (median). Conclusion: Bronchoscopic ethanolamine injection therapy may be partially useful in controlling air leakage, and reducing the hospital stay in patients with persistent air leak from CTD.
( Ah Leum Lim ),( Cheol Hong Kim ),( In Gyu Hyun ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1
Background: Chest tube drainage (CTD) has indicated for the treatment of pneumothorax, hemothroax and after thoracic surgery. But, in the case of incomplete lung expansion and/or persistent air leak from CTD, medical or surgical thoracoscopy or, if that is unavailable, limited thoracotomy should be considered. We evaluate the efficacy of bronchoscopic injection of absolute ethanolamine to control persistent air leak in patients with CTD. Methods: Patients who had persistent or prolonged air leak from CTD were included consecutively. We directly injected 1.0 ml aliquots of ethanolamine into a subsegmental or its distal bronchus where is probably air leakage site, 1 to 20 times using injection needle through a fiberoptic bronchoscope. Results: 15 patients (all men) were enrolled. There were 14 spontaneous pneumothorax (5 idiopathic, 6 chronic obstructive pulmonary disease (COPD) and 3 post-tuberculosis) and 1 empyema associated with broncho-pleural fistula in the study. Of 14 patients with ethanolamine injection therapies, five had previous surgical therapy, wedge resection for bullae, but the others didn`t have. Twelve were successfully treated by an ethanolamine injection therapy alone. But three (idiopathic, COPD and post-tuberculosis) were failed and followed by a surgery (2 cases) or pleurodesis (1 case). Minor complications such as fever, chest pain and transient pneumonic infiltrations occurred after the therapy. With successful, the time to discharge was about 3 days (median). Conclusions: Bronchoscopic ethanolamine injection therapy may be partially useful in controlling air leakage and reducing the hospital stay in patients with CTD.
Lim, Ah-Leum,Kim, Cheol-Hong,Hwang, Yong-Il,Lee, Chang-Youl,Choi, Jeong-Hee,Shin, Tae-Rim,Park, Yong-Bum,Jang, Seung-Hun,Park, Sang-Myeon,Kim, Dong-Gyu,Lee, Myung-Goo,Hyun, In-Gyu,Jung, Ki-Suck,Shin, The Korean Academy of Tuberculosis and Respiratory 2012 Tuberculosis and Respiratory Diseases Vol.72 No.5
Background: Chest tube drainage (CTD) is an indication for the treatment of pneumothorax, hemothroax and is used after a thoracic surgery. But, in the case of incomplete lung expansion, and/or persistent air leak from CTD, medical or surgical thoracoscopy or, if that is unavailable, limited thoracotomy, should be considered. We evaluate the efficacy of bronchoscopic injection of ethanolamine to control the persistent air leak in patients with CTD. Methods: Patients who had persistent or prolonged air leak from CTD were included, consecutively. We directly injected 1.0 mL solution of 5% ethanolamine oleate into a subsegmental or its distal bronchus, where it is a probable air leakage site, 1 to 21 times using an injection needle through a fiberoptic bronchoscope. Results: A total of 15 patients were enrolled; 14 cases of spontaneous pneumothorax [idiopathic 9, chronic obstructive pulmonary disease (COPD) 3, post-tuberculosis 2] and one case of empyema associated with broncho-pleural fistula. Of these, five were patients with persistent air leak from CTD, just after a surgical therapy, wedge resection with plication for blebs or bullae. With an ethanolamine injection therapy, 12 were successful but three (idiopathic, COPD and post-tuberculosis) failed, and were followed by a surgery (2 cases) or pleurodesis (1 case). Some adverse reactions, such as fever, chest pain and increased radiographic opacities occurred transiently, but resolved without any further events. With success, the time from the procedure to discharge was about 3 days (median). Conclusion: Bronchoscopic ethanolamine injection therapy may be partially useful in controlling air leakage, and reducing the hospital stay in patients with persistent air leak from CTD.
음소별 낱말을 이용한 음운규칙 중재가 학령전기 아동의 해독능력에 미치는 효과
금아름(Ah-Leum Keum),허승덕(Seung-Deok Heo) 단국대학교 특수교육연구소 2020 특수교육논총 Vol.36 No.3
연구목적: 읽기 능력은 학업의 기초이며 학령전기부터 학령기까지 가장 중요한 과업중 하나이다. 이 시기 아동들은 특히 자소-음소 불일치 단어들을 이해하는데 어려움을 보이며, 능숙한 읽기를 위해서는 음운규칙이 적용되는 음운변동 낱말들의 읽기 훈련이 필요하다. 읽기 문제가 없는 성인은 철자정보와 낱말정보를 동시에 떠올리면서 의미 해독을 자동적으로 진행하고, 뜻을 이해할 수 있다. 이 때 자동적으로 의미가 활성화되는 단어를 일견단어(sight word)라고 하며, 일견단어 정보량에 따라 읽기유창성이 달라진다. 이 연구의 목적은 규칙성 있는 훈련단어를 이용한 음운규칙 해독훈련이 단어 해독에 미치는 영향을 알아보는데 있다. 연구방법: 연구대상은 생활연령이 5~6세인 학령전기 아동 4명을 대상으로 하였다. 훈련단어는 사용빈도와 규칙성을 고려하여 음운규칙을 정하고 각 음소별로 나누어 사용하였다. 연구는 기초선, 중재, 유지 순으로 진행하였고, 중재는 주 2회 제공하였다. 목표 낱말은 수행률이 80%이상이면 다음 목표낱말로 이행하였다. 수행률은 백분위로 환산하여 평가하였다. 연구결과: 수행률은 기초선 11.3%에서 유지평가 54.20%로 개선되었다. 유지평가 수행률은 대상자마다 74.3%, 61.1%, 33.3%, 48.1% 등으로 차이가 있었으나 전반적인 향상이 확인되었다. 결론: 음소별로 훈련단어를 이용한 음운규칙훈련 중재는 자소-음소 불일치 단어의 일견단어의 정보량을 늘려주어 음운규칙 해독능력 향상에 긍정적인 영향이 있었다. Purpose: Reading ability is the foundation of academics and is one of the most important tasks from preschool to school ages. During this period children have especial difficulties in understanding Korean phonological process words, so it is necessary for children to have training in reading to enhance their reading ability. Adults who do not have any reading difficulties can automatically interpret the meaning of words by simultaneously recalling spelling information and words information and understand the meaning. Sight word refers to a word of which the meaning is automatically activated, and the reading proficiency differs depending on the amount of sight word. This study aimed to investigate the effect of phonological rules training that uses training words to interpret words. The participants were four preschool children aged from five to six years. Method: Training words were divided according to their respective phonemes based on the frequency of use and regularity. The study proceeded in the sequence of baseline, mediation, and maintenance where mediation was provided twice a week. If the performance of the target words exceeded 80%, the next target words were used. The performance rate was evaluated in percentiles. Result: The performance improved from the baseline of 11.3% to the maintenance evaluation of 54.20%. Although the maintenance evaluation performance rate differed among participants as 74.3%, 61.1%, 33.3%, 48.1%, and more, there exists an overall improvement. Conclusion: Phonological rules training using wards sorted by phonemes had a positive influence on enhancing the phonological rules decoding by increasing the amount of information of sight word in the Korean phonological process words.