Susceptibility Loci Associations with Prostate Cancer Risk in Northern Chinese Men

Wang, Na-Na,Xu, Yong,Yang, Kuo,Wei, Dong,Zhang, Yao-Guang,Liu, Ming,Shi, Xiao-Hong,Liang, Si-Ying,Sun, Liang,Zhu, Xiao-Quan,Yang, Yi-Ge,Tang, Lei,Zhao, Cheng-Xiao,Wang, Xin,Chen, Xin,Hui, Juan,Zhang, Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: KLK3 gene products, like human prostate-specific antigen (PSA), are important biomarkers in the clinical diagnosis of prostate cancer (PCa). G protein-coupled receptor RFX6, C2orf43 and FOXP4 signaling plays important roles in the development of PCa. However, associations of these genes with PCa in northern Chinese men remain to be detailed. This study aimed to investigate their impact on occurrence and level of malignancy. Methods: All subjects were from Beijing and Tianjin, including 266 cases with prostate cancer and 288 normal individuals as controls. We evaluated associations between clinical covariates (age at diagnosis, prostate specific antigen, Gleason score, tumor stage and aggressive) and 6 candidate PCa risk loci, genotyped by PCR- high resolution melting curve and sequencing methods. Results: Case-control analysis of allelic frequency of PCa associated with PCa showed that one of the 6 candidate risk loci, rs339331 in the RFX6 gene, was associated with reduced risk of prostate cancer (odds ratio (OR) = 0.73, 95% confidence interval (CI) =0.57-0.94, P = 0.013) in northern Chinese men. In addition, subjects with CX (CC+TC) genotypes had a decreased risk for prostrate cancer compared to those carrying the TT homozygote (OR =0.64, 95% CI = 0.45- 0.90, P = 0.008). The TT genotype of 13q22 (rs9600079, T) was associated with tumor stage (P=0.044, OR=2.34, 95% CI=0.94-5.87). Other SNPs were not significantly associated with clinical covariates in prostate cancer (P > 0.05). Conclusions. rs339331 in the RFX6 gene may be associated with prostate cancer as a susceptibility locus in northern Chinese men.

Antiviral Activities of Natural Compounds Derived from Traditional Chinese Medicines against Porcine Circovirus Type 2 (PCV2)

Na Sun,Tong Yu,Jun-Xing Zhao,Yaogui Sun,Junbing Jiang,Zhi-Bian Duan,WenkuiWang,Yuan-Liang Hu,Hai-Min Lei,HongquanLi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Twenty natural compounds isolated fromtraditional Chinese medicines were examined for theirantiviral activities against PCV2 in vitro. Antiviral activitywas analyzed by the assays of blocking of virus entry,inhibition of virus replication and inactivation of virusparticles in the virus suspension. 50% cytotoxic concentration(CC50) and 50% effective concentration (EC50) for eachcompound were determined by using the MTT method andimmunofluorescence assay (IFA), respectively. The resultsobtained from the inhibition of virus replication andinactivation of virus particles showed that the maximuminhibition rates for matrine and scutellarin were 57 and72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virusentry and inhibition of virus replication demonstrated thatboth aesculetin and arecoline hydrochloride stimulatedPCV2 replication, with the maximum stimulation rate of387.3 and 219.4%. In summary, both matrine and scutellarinhad antiviral activity against PCV2, while aesculetin andarecoline hydrochloride promoted PCV2 infection replicationin vitro.

Methylation-associated silencing of BASP1 contributes to leukemogenesis in t(8;21) acute myeloid leukemia

Lei Zhou,Lin Fu,Na Lv,Jing Liu,Yan Li,Xiaosu Chen,Qingyu Xu,Guofeng Chen,Baoxu Pang,Lili Wang,Yonghui Li,Xiaodong Zhang,Li Yu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The AML1-ETO fusion protein (A/E), which results from the t(8;21) translocation, is considered to be a leukemiainitiating event. Identifying the mechanisms underlying the oncogenic activity of A/E remains a major challenge. In this study, we identified a specific down-regulation of brain acid-soluble protein 1 (BASP1) in t(8;21) acute myeloid leukemia (AML). A/E recognized AML1-binding sites and recruited DNA methyltransferase 3a (DNMT3a) to the BASP1 promoter sequence, which triggered DNA methylation-mediated silencing of BASP1. Ectopic expression of BASP1 inhibited proliferation and the colony-forming ability of A/E-positive AML cell lines and led to apoptosis and cell cycle arrest. The DNMT inhibitor decitabine up-regulated the expression of BASP1 in A/E-positive AML cell lines. In conclusion, our data suggest that BASP1 silencing via promoter methylation may be involved in A/E-mediated leukemogenesis and that BASP1 targeting may be an actionable therapeutic strategy in t(8;21) AML.

Dendritic porous SnO2/SiO2@polymer nanospheres for pH-controlled styptic drug release

Na Cao,Xin Xie,Yu Zhang,Yanbao Zhao,Liuqin Cao,Lei Sun 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-

Dendritic porous tin oxide/silica (DTS) nanospheres (NSs) were prepared by emulsion-condensationroute. DTS NSs have an average diameter of 80 nm and possess accessible center-radial large porechannels, which endow them high surface area, less drug diffusion resistance and more mass transport. SnO2 quantum dots (QDs) embedded in silica skeleton can be used to track the drug release behavior. DTS NSs were coated by chitosan/polymethacrylic acid and polymer shell would make them achievegreat loading capacity and pH-sensitive release for aminomethylbenzoic acid (AMBA).

The Role of Splicing Factors in Adipogenesis and Thermogenesis

Lei Sun,Yadanar Than Naing 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.5

Obesity is a significant global health risk that can cause a range of serious metabolic problems, such as type 2 diabetes and cardiovascular diseases. Adipose tissue plays a pivotal role in regulating energy and lipid storage. New research has underlined the crucial role of splicing factors in the physiological and functional regulation of adipose tissue. By generating multiple transcripts from a single gene, alternative splicing allows for a greater diversity of the proteome and transcriptome, which subsequently influence adipocyte development and metabolism. In this review, we provide an outlook on the part of splicing factors in adipogenesis and thermogenesis, and investigate how the different spliced isoforms can affect the development and function of adipose tissue.

An Open-switch Fault Diagnosis Method for Single-phase PWM Rectifier Based on CEEMD-DNN

Na Qin,Tianwei Wang,Deqing Huang,Yiming Zhang,Lei Ma 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

Based on complementary ensemble empirical mode decomposition and deep neural network (CEEMDDNN), a novel diagnosis method is proposed to discover the open-circuit faults of insulated gate bipolar transistors (IGBTs) in single-phase pulse width modulation (PWM) AC-DC rectifier, an important part of traction power supply system of high-speed railway. By virtue of the combination of signal processing and deep learning schemes, the multi-scale feature information for IGBT’s open-circuit fault diagnosis are extracted by regarding the input current of rectifier as the original signal. More clearly, CEEMD is adopted to decompose the original signal to a series of intrinsic mode function components (IMFs). Then, the correlation coefficient algorithm is used to evaluate the correlation between each of the IMFs and the original signal. Further, those IMFs that have been verified to be highly correlated with the original signal are selected as the input eigenvectors of DNN to train the IGBTs fault diagnosis network. Experimental results show that the proposed CEEMD-DNN algorithm is superior to pure DNN, empirical mode decomposition (EMD)-DNN and ensemble empirical mode decomposition (EEMD)-DNN in the sense that within the similar time-consuming situations the fault diagnosis accuracy has been improved from 73.0%, 86.0%, and 89.9% to almost 99.0%. This is mainly owing to the remarkable decomposing performance of CEEMD for complex mixed signal, and the strong feature extraction and learning abilities of DNN in pattern recognition.

Clinical Study of Tumor Angiogenesis and Perfusion Imaging Using Multi-slice Spiral Computed Tomography for Breast Cancer

Xu, Na,Lei, Zhen,Li, Xiao-Long,Zhang, Jun,Li, Chen,Feng, Guo-Quan,Li, Di-Nuo,Liu, Jing-Yi,Wei, Qiang,Bian, Ting-Ting,Zou, Tian-Yu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Objectives: To explore the correlation between multi-slice spiral CT (MSCT) perfusion parameters and the expression of vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase-2 (MMP-2) in breast cancer. Methods: Forty five breast cancer patients and 16 patients with benign breast tumor, both confirmed by pathology examination, were enrolled. All underwent MSCT perfusion imaging to obtain perfusion maps and data for parameters including blood flow (BF), blood volume (BV) and permeability surface (PS). Cancer patients did not receive treatment prior to surgery. The expression of VEGF and MMP-2 were examined with both immunohistochemistry and Western blotting. Results: The levels of VEGF and MMP-2 by immunohistochemistry were significantly higher in the breast cancer group (P < 0.01) than the benign tumor group. Relative OD values from Western blotting were also higher in cancer cases (P < 0.05). Similarly, the mean MSCT perfusion parameters (BF, BV, PS) were significantly higher in the breast cancer group (P < 0.01), BF and BV positively correlating with VEGF expression (r = 0.878 and 0.809 respectively, P < 0.01); PS and VEGF and MMP-2 expression were also positively correlated (r= 0.860, 0.786 respectively, P < 0.01). Conclusion: There is a correlation between breast cancer MSCT perfusion parameters and VEGF andMMP-2 expression, which might be useful for detection of breast lesions, qualitative diagnosis of breast cancer, and evaluation of breast cancer treatment.

Aberrant Expression of Markers of Cancer Stem Cells in Gastric Adenocarcinoma and their Relationship to Vasculogenic Mimicry

Zhou, Lei,Yu, Lan,Feng, Zhen-Zhong,Gong, Xiao-Meng,Cheng, Ze-Nong,Yao, Nan,Wang, Dan-Na,Wu, Shi-Wu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.

Analog Filter Circuits Feature Selection Using MRMR and SVM

Yongkui Sun,Lei Ma,Na Qin,Meilan Zhang,Qianyong Lv 제어로봇시스템학회 2014 제어로봇시스템학회 국제학술대회 논문집 Vol.2014 No.10

This paper addresses frequency response feature extraction of analog filter circuits. An approach for feature selection using criteria of maximum relevance minimum redundancy (MRMR) and support vector machine (SVM) is proposed. Key idea of the method is to obtain candidate feature subsets with descending order using criteria of MRMR first, and then to select the optimal feature subset through cross validation results of each feature subset using SVM. Experimental results testify that the proposed approach has the advantages of less time-consuming and the selected features have reasonable distribution.

Calcination and sintering effects on the microstructure and dielectric properties of CaCu3Ti4O12 ceramics

Peng Liu,Lei Cao,Jian-Ping Zhou,Ya-Juan Wang,Li-Na Su,Cheng Liu,Huai-Wu Zhang 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.4

CaCu3Ti4O12 (CCTO) ceramics were fabricated by a conventional solid-state reaction at various calcination and sintering temperatures. Depending on the relative dielectric constant (εr) at 1 kHz, the specimens can be categorized into three types:type A (εr < 5 × 104), type B (5 × 104 < εr < 105) and type C (εr > 105). The value of εr is closely related to the microstructure:type-A specimens show a morphology with large grains embedded in a small-grained matrix, type-B specimens present a network structure and type-C specimens display a core-shell structure. Different dielectric loss variation tendencies are observed in the type-A specimens, which can be well explained by impedance spectroscopy analysis and further confirmed by the EDX results. Also, the proper calcination and sintering temperature ranges needed to achieve good comprehensive properties are given.