Recombinant-attenuated Salmonella Pullorum strain expressing the hemagglutinin-neuraminidase protein of Newcastle disease virus (NDV) protects chickens against NDV and Salmonella Pullorum challenge

Ke Ding,Ke Shang,Zu-Hua Yu,Chuan Yu,Yan-Yan Jia,Lei He,Cheng-Shui Liao,Jing Li,Chun-Jie Zhang,Yin-Ju Li,Ting-Cai Wu,Xiang-chao Cheng 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2

Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccinecan protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase(HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologousrecombination method to form a new bivalent vaccine candidate against Newcastle disease (ND) and S. Pullorum disease (PD). The effectof this vaccine candidate was compared with those of the NDV LaSota and C79-13ΔcrpΔasd (pYA) strains. The serum hemagglutinationinhibition antibody titers, serum immunoglobulin G (IgG) antibodies, secretory IgA, and stimulation index in lymphocyte proliferation wereincreased significantly more (p < 0.01) in chickens inoculated with C79-13ΔcrpΔasd (pYA-HN) than with C79-13ΔcrpΔasd (pYA) but werenot significantly increased compared with the chickens immunized with the LaSota live vaccine (p > 0.05). Moreover, the novel strain provides60% and 80% protective efficacy against the NDV virulent strain F48E9 and the S. Pullorum virulent strain C79-13. In summary, in this study,a recombinant-attenuated S. Pullorum strain secreting NDV HN protein was constructed. The generation of the S. Pullorum C79-13ΔcrpΔasd(pYA-HN) strain provides a foundation for the development of an effective living-vector double vaccine against ND and PD.

연구논문 : 한국 연예인 광고모델 속성이 중국 의류제품과 전자제품 광고효과에 미치는 영향

뢰가 ( Ke Lei ),김한나 ( Han Na Kim ) 한국의류학회 2014 한국의류학회지 Vol.38 No.3

This study examined the effects of Korean celebrity advertising models on the advertising effectiveness for Chinese brands by comparing apparel products with electronic products. In addition, this study explored the effects of gender and monthly expenses on the evaluation of Korean celebrity advertising models. This study targeted 388 Chinese university students in China. The results show that Korean advertising models consist of five attributes (familiarity, visibility, credibility, similarity, and attractiveness) and that advertising effectiveness consists of three factors (attitude toward the advertisement, attitude toward the brand, and purchase intention). The impact of these five advertising model attributes on advertising effectiveness differ for apparel and electronic products. The results also indicate gender and expense differences in the evaluation of Korean celebrity models. We discuss the theoretical and managerial implications of the results along with potential limitations and future research directions.

Internal force monitoring design of long span bridges based on ultimate bearing capacity ratios of structural components

Hu, Ke,Xie, Zheng,Wang, Zuo-Cai,Ren, Wei-Xin,Chen, Lei-Ke Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.1

In order to provide a novel strategy for long-span bridge health monitoring system design, this paper proposes a novel ultimate bearing capacity ratios based bridge internal force monitoring design method. The bridge ultimate bearing capacity analysis theories are briefly described. Then, based on the ultimate bearing capacity of the structural component, the component ultimate bearing capacity ratio, the uniformity of ultimate bearing capacity ratio, and the reference of component ultimate bearing capacity ratio are defined. Based on the defined indices, the high bearing components can then be found, and the internal force monitoring system can be designed. Finally, the proposed method is applied to the bridge health monitoring system design of the second highway bridge of Wuhu Yangtze river. Through the ultimate bearing capacity analysis of the bridge in eight load conditions, the high bearing components are found based on the proposed method. The bridge internal force monitoring system is then preliminary designed. The results show that the proposed method can provide quantitative criteria for sensors layout. The monitoring components based on the proposed method are consistent with the actual failure process of the bridge, and can reduce the monitoring of low bearing components. For the second highway bridge of Wuhu Yangtze river, only 59 components are designed to be monitored their internal forces. Therefore, the bridge internal force monitoring system based on the ultimate bearing capacity ratio can decrease the number of monitored components and the cost of the whole monitoring system.

A worldwide species catalogue and key of Sermyloides (Coleoptera, Chrysomelidae, Galerucinae) with description of two new species from China

Lei Qi-long,Yang Xing-ke,Nie Rui-e 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

In this study, a species catalogue of Sermyloides, including 42 species, is given. Two new species are described: Sermyloides wuyiensis sp. nov. from Fujian, China and Sermyloides bipunctatus sp. nov. from Chongqing, China. A key to species, photographs of aedeagus, habitus of new species and habitus of 14 species of Chinese holotypes are provided.

Early Efficacy of Taxotere and Cisplatin Chemo-Radiotherapy for Advanced Cervical Cancer

Ke, Qing-Hua,Zhou, Shi-Qiong,Du, Wei,Lei, Yong,Huang, Min,Luo, Fei,Yang, Ji-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

The aim of this study was to investigate the early outcome of the taxotere and cisplatin chemoradiotherapy for advanced cervical cancer. Fifty-six cases (FIGO II b to IVa) were divided randomly into two groups: radiotherapy alone (28 cases) and radiation plus chemotherapy (TP) group. There was no difference in radiotherapy between the two groups. The RT+C cases who received TP regimen during the radiation, and DDP once weekly injection of vain, according to 20$mg/m^2$ and taxotere once weekly iv according to 35 $mg/m^2$. These regimens were given for 4~5weeks, and some medicines to control vomiting were available for the RT+C cases. The two groups received an oral medicine MA 160mg every day during the treatment. Regarding early outcome, the complete remission rate was 64.3% and partial remission rate was 35.7% in RT+C. The complete remission rate was 32.1% and partial remission rate was 39.3% in RT. The total response rate and complete remission in the RT+C group were higher than that in the RT group. We conclude that taxotere and cisplatin chemoradiotherapy can improve the early outcome of the advanced cervical cancer, the adverse effects being endurable.

Early Efficacy of Endostar Combined with Chemoradiotherapy for Advanced Cervical Cancers

Ke, Qing-Hua,Zhou, Shi-Qiong,Huang, Min,Lei, Yong,Du, Wei,Yang, Ji-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

The aim of this study was to investigate the early outcome of Endostar combined with chemoradiotherapy for advanced cervical cancer. Fifty-two cases (FIGO IIb to IVa) were divided randomly into two groups, receiving chemoradiotherapy alone (CRT group) and Endostar combined with chemoradiotherapy (CRT+E group). For the patients in the CRT+E group, Endostar was administered daily with the dosage of 7.5 $mg/m^2$, and cisplatin was administered weekly with the dosage of 20 $mg/m^2$ during the radiation. The regimens lasted for 4 weeks with no difference in chemoradiotherapy between the two groups. The early outcome complete remission rate was 73.1%, partial remission rate was 23.1% and the total response rate was 96.2% in CRT+E group, a significant improvement on the 34.6%, 42.3% and 76.9%, respectively, in the CRT group. One year survive rates were 100% and 84.6% in the CRT+E group and CRT groups, the difference being significant. Endostar combined with chemoradiotherapy can improve the early outcome of the advanced cervical cancer, and adverse effects were not encountered.

Disassembly Line Balancing Optimization Method for High Efficiency and Low Carbon Emission

Lei Zhang,Xikun Zhao,Qingdi Ke,Wanfu Dong,Yanjiu Zhong 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.1

Disassembly is the first step in product recycling and remanufacturing. When disassembling large quantities of products, the disassembly efficiency is crucial for enterprises. Thus, disassembly line is the best choice for automated disassembly of disposal products. Disassembling products, especially complex structure of products, consume a lot of resources and energy and produce large amounts of carbon emissions. So, it is essential for disassembly line to work efficiently and environmentally. In this paper, the hybrid graph is proposed to express the direct and indirect constraint relationship among components. Then, the mathematical model of carbon emission is built by quantifying the carbon emissions in the process of product disassembly. Taking into account the basic disassembly time, direction change time and tool change time, the mathematical model of high efficiency is presented for optimizing disassembly time. Additionally, based on the traditional multi-objective disassembly line balancing problem (DLBP), a novel multi-objective optimization model of the DLBP with shortest disassembly time and minimum carbon emissions is proposed for improving disassembly efficiency and reducing the carbon emissions in the process of disassembly. Furthermore, genetic algorithm is presented for optimizing the disassembly sequence. Finally, an automobile engine is given as an example to confirm the practicality of the proposed model in solving the DLBP.

Comparative study of thermal gelation properties and molecular forces of actomyosin extracted from normal and pale, soft and exudative-like chicken breast meat

Ke Li,Jun-Ya liu,Lei Fu,Ying-Ying Zhao,Yan-Hong Bai 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.5

Objective: The objectives of this study were to investigate the thermal gelation properties and molecular forces of actomyosin extracted from two classes of chicken breast meat qualities (normal and pale, soft and exudative [PSE]-like) during heating process to further improve the understanding of the variations of functional properties between normal and PSE-like chicken breast meat. Methods: Actomyosin was extracted from normal and PSE-like chicken breast meat and the gel strength, water-holding capacity (WHC), protein loss, particle size and distribution, dynamic rheology and protein thermal stability were determined, then turbidity, active sulfhydryl group contents, hydrophobicity and molecular forces during thermal-induced gelling formation were comparatively studied. Results: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed that protein profiles of actomyosin extracted from normal and PSE-like meat were not significantly different (p>0.05). Compared with normal actomyosin, PSE-like actomyosin had lower gel strength, WHC, particle size, less protein content involved in thermal gelation forming (p<0.05), and reduced onset temperature (To), thermal transition temperature (Td), storage modulus (G′) and loss modulus (G″). The turbidity, reactive sulfhydryl group of PSE-like actomyosin were higher when heated from 40°C to 60°C. Further heating to 80°C had lower transition from reactive sulfhydryl group into a disulfide bond and surface hydrophobicity. Molecular forces showed that hydrophobic interaction was the main force for heat-induced gel formation while both ionic and hydrogen bonds were different significantly between normal and PSE-like actomyosin (p<0.05). Conclusion: These changes in chemical groups and inter-molecular bonds affected protein-protein interaction and protein-water interaction and contributed to the inferior thermal gelation properties of PSE-like meat.

Design and torque calculation of permanent magnet eddy current coupling with sinusoidal torque output

Ke Chen,Hongyang Shi,Yajun Guo,Lei Yin,Ziwei Bao,Hongmei Zheng 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.4

Due to its unique characteristic of non-contact torque transmission between its permanent magnetic rotor and conductorrotor, permanent magnet eddy current coupling (PMECC) is preferable in transmitting torque from the outside to the insideof a sealed container, which can effectively avoid the tricky problem of dynamic seals. To mix non-uniform liquid solutionsor gas mixtures in sealed containers more effectively, a sinusoidal torque is also required. Thus, permanent magnet eddy current coupling with a slotted conductor is proposed in this paper, which can adaptively output sinusoidal torque under a real-time load when the input rotational speed is constant. Based on the equivalent magnetic circuit method, an analytical model for calculating torque is proposed. When establishing the equivalent magnetic circuit model of the PMECC, the radial magnetic flux leakage circuit is added to the equivalent magnetic circuit diagram, and the cross-sectional shape of the eddy current region is equivalent to an octagon, which simplifies the torque calculation process. The analytical model is verified by finite element method (FEM) simulation and experiments. According to a comparison, the peak and lowest values of output torque obtained by the experiments, the analytical model, and the FEM simulation are consistent. The output torque waveform obtained by the analytical model and FEM simulation are also consistent.

Effect of bamboo shoot dietary fiber on gel properties, microstructure and water distribution of pork meat batters

Li Ke,Liu Jun-Ya,Fu Lei,Zhao Ying-Ying,Zhu He,Zhang Yan-Yan,Zhang Hua,Bai Yan-Hong 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.7

Objective: To develop healthier comminuted meat products to meet consumer demand, the gel properties, rheological properties, microstructure and water distribution of pork meat batters formulated with various amounts of bamboo shoot dietary fiber (BSDF) were investigated. Methods: Different levels of BSDF (0% to 4%) were added to pork batters, and the pH, color, water-holding capacity, texture and rheological properties of pork batters were determined. Then, pork batters were analyzed for their microstructure and water distribution using scanning electron microscopy (SEM) and low-field nuclear magnetic resonance (LF-NMR). Results: Compared with the control, BSDF addition into meat batters showed a significant reduction in L*-value and a significant increase in b*-value (p<0.05). BSDF addition of up to 4% reduced the pH value of pork batters by approximately 0.15 units; however, the cooking loss and expressible water loss decreased significantly (p<0.05) with the increased addition of BSDF. The hardness and gel strength were noticeably enhanced (p<0.05) as the content of BSDF increased. The rheological results showed that BSDF added into pork batters produced higher storage modulus (G′) and loss modulus (G″) values. The SEM images suggested that the addition of BSDF could promote pork batters to form a more uniform and compact microstructure. The proportion of immobilized water increased significantly (p<0.05), while the population of free water was decreased (p<0.05), indicating that BSDF improved the water-holding capability of pork batters by decreasing the fraction of free water. Conclusion: BSDF could improve the gel properties, rheological properties and water distribution of pork meat batters and decrease the proportion of free water, suggesting that BSDF has great potential as an effective binder in comminuted meat products.