Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

백혈병 세포주에 대한 (±)-ar-Turmerone, 자근 및 황금추출물에 의한 항암제의 세포독성 증강효과

이윤영,유관희,김삼용,안병준 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

Using the colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide](MTT)assay, we evaluated the chemosensitivity of 8 anticancer drugs{vincristine(VCR), vinblastine(VBL), adriamycin(ADR), cisplatin(CPDD), etoposide(VP-16), cytosine arabinoside(ara-C), bleomycin(Bleo) and cyclophosphamide(CYC)} and the cytotoxicity-enhancing effects of (±)-ar-turmerone and the extracts of the crude drugs {Lithospermum erythrorhizon(LE) and Scutellaria baicalensis(SB)}on the above mentioned anticancer drugs against HL-60 and KG-1 cells among 8 anticancer drugs, VCR, VBL, ADR, and CPDD inhibited the growth of both cell lines by more than 50% while VP-16, ara-C, Bleo, and CYC were less effective. (±)-ar-Turmerone had significant inhibitory effects against both cell lines, showing the ID_50 values of 11.730 ㎍/㎖ and 0.292 ㎍/㎖ for HL-60 and KG-1 cells. respectively. But the extracts of LE and SB roots showed no significant cytotoxic effects. According to ID_50 values, the cytotoxicities of VCR, VBL and ADR against HL-60 were enhanced two, eight and three times by mixing (±)-ar-turmerone, five, seven and three times by adding the extract of LE root, and twenty, six and three times by mixing the extract of SB root, respectively. The cytotoxicities of the above mentioned drugs against KG-1 cell were enhanced two, seven and three times by mixing (±)-ar-turmerone, two, three and three times by conbining wilth the extract of LB root, and two, five and two times by adding the extract of SB root, respectively. The cytotoxicity-potentiating effects of (±)-ar-turmerone and the extracts of LE and SB roots against HL-60 cell were greater than KG-1 cell.

부르따뉴의 부활

이산호 프랑스문화예술학회 1999 프랑스문화예술연구 Vol.1 No.-

La Bretagne est le pays de la mer, des forets mystherieux dt des legendes. Jusqu'au milieu du XVIIIe siecle, ce pays dont les habitants qui formaient un etat different du reste de la France par le genie, les moeurs et la langue, a reste pEr connu dans l'histoire et la litterature. Les oeuvres de Chateaubriand sont pleines de l'ame bretonne. Dans les Martyrs dont queques descriptions concernent le paysage breton et les environs du chateau paternel, Chateaubriand a fait renaitre la vie meme de ses aieux, leurs caracteres, leurs moeurs et leur culte druidique. La Bretagne a recu pour longtemps d'un de ses plus illustres fils, Chateaubriand son image. Avec sa couleur historique et locale, le tableau de la Bretagne dans les Martyrs marque une etape interessante de la formation des idees de l'ecole romantique. Poete-historien, Chateaubriand a cree L'histoire romantique avec la description vivante des tableaux historiques, et il a fait entrer pour la premiere fois son pays natal, la Bretagne, dans la litterature francaise. Apres lui, elle a tenu une place importante dans l'histoire francaise, grace a Renan, Lamennais, Michelet et Augustin Tierry.

정신질환자의 계속입원치료 심사에서 불승인에 미치는 요인

이산수,오소영,박민철 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

정신보건법에 의한 계속입원치료 심사청구는 해마다 증가하고 있다. 이에 계속입원치료 심사의 청구서에 기재되어 있는 항목들을 바탕으로 일 지역 정신보건심판위원회의 계속입원치료 불승인 현황과 그 불승인에 영향을 주는 요인에 대해 조사하였다. 2002년 1월부터 2006년 12월 까지 5년간 정선보건심판위원회에 요청된 계속입원치료 심사청구는 총 14,782 건이었다. 이 중 불승인은 1,832건으로 약 12.4%의 불승인율을 보였다. 각 요인별 불승인율을 보면, 시설은 정신병원 10.6%, 정신요양원 14.6%이었고, 의료보장 형태는 의료보험 10.5%, 의료보호 12.9%이었고 모두 유의하게 차이가 있었다. 진단에 따른 불승인율은 알코올 중독 27.9%, 기타 17.1%, 정신지체 16.4%, 기분장애 12.5%, 정신분열병 11.5%, 기질성 정신장애 8.6% 순이었으며 유의한 차이가 있었다. 계속입원치료 심사청구 횟수와 진단에 따른 불승인율을 보면 기분장애, 정신지체, 기타 환자들은 3회-6회 사이에서 불승인율이 높았다. 심사청구 횟수와 시설을 보면, 정신병원은 4회 이상에서 정신요양원은 6회 이상에서 불승인율이 높았다. 계속입원치료 심사에 가장 영향을 주는 요인은 시설, 심사청구 횟수, 진단이었으며 주로 정신병원 입원, 알코올중독, 의료보험, 자해 및 타해의 위험성이 낮고 지남력이 좋은 환자들에서 불승인이 되는 경우가 많았다. The number of applications for judgment of continuing hospitalization by the Mental Health Act is increasing every year. Thus, the present study surveyed the current state of continuing hospitalization disapproval by the Mental Health Judgment Board of an area, and factors affecting the disapproval based on items recorded in the application for the judgment of continuing hospitalization. During the 5 years' period from January 2002 to December 2006, there were a total of 14,782 applications for judgment of continuing hospitalization to the Mental Health Judgment Board. Among them, 1,832were disapproved, showing a disapproval rate of around 12.4%. As to disapproval rate related to each factor, according to facility, the disapproval rate was 10.6% for patients at mental hospitals and 14.6% for those at nursing homes for mental patients. According to medical security type, it was 10.5% for medical insurance patients and 12.9% for medical aid patients. These differences were all statistically significant. According to diagnosis, the disapproval rate was 27.9% for alcoholism, 17.1% for others, 16.4% for mental retardation, 12.5% for mood disorder, 11.5% for schizophrenia, and 8.6% for organic mental disorder, and the differences were statistically significant. According to the number of applications for judgment of continuing hospitalization and diagnosis, the disapproval rate was higher when the number of applications was 3~6 among patients with mood disorder, mental retardation, or others. According to the number of applications for review and facility, the disapproval rate was higher when number of applications was 4 or more among mental hospital patients, and when it was 6 or more among nursing home patients. The factors most influential on the judgment of continuing hospitalization were facility, the number of applications for review, and diagnosis, and the disapproval rate was high among mental hospital patients, alcoholics, those with medical insurance, and those with low risk of self-injury and injury by others and good orientation.

컴퓨터에서 쉽게 즐길 수 있는 비행 슈팅게임의 설계

강상우, 김지윤, 안성옥, 김수균, 박동원 배재대학교 공학연구소 2014 공학논문집 Vol.16 No.1

슈팅 게임(Shoting game)은 액션게임에 속해 있는 장르중 하나로 총 또는 미사일 같은 원거리 무기를 사용해 공격과 적의 공격을 피하는 게임이며[1]‘비행슈팅(Aircraft Shooting)’은 비행기를 조작해서 싸우는 게임이다[2]. 비행슈팅게임은 누구나 게임에 대해 쉽게 이해하고, 쉽게 즐길 수 있기 때문에 다른 장르의 게임들에 비해 대중화 되어 있다. 본 게임은 컴퓨터에서 쉽게 즐길 수 있도록 설계된 게임으로 우주를 배경으로 제작된 게임이며, 수많은 운석과 기뢰들을 피하고, 게임 중반부에 나오는 해적선을 파괴하고 목적지까지 도달하는 것이 게임의 목표이다. 본 게임은 Unity3D를 이용하였고, Unity3D엔진은 다른 플랫폼과의 호완성이 좋고 자체 내에서 지원해주는 기능들이 많기 때문에 많은 개발자들이 사용하고 있다.

MIMO 시스템에서의 APP 디코딩의 성능

이경규,김산해,김홍철,이원철,신요안 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

MIMO (multi-input multi-output) systems can achieve high data capacity and high data rate in the third generation (3G) or beyond-3G mobile communication systems. To effectively improve the performance of MIMO systems, new detection schemes which can mitigate mutual interferences from multiple transmit antennas, are highly required. In this paper, we consider a posteriori probability (APP) type detection schemes for the MIMO systems, and compare with other sub-optimal detection schemes based on zero forcing (ZF) and minimum mean square error (MMSE) - ordered successive interference cancellation (OSIC). Simulation results show that the APP outperforms ZF-OSIC and MMSE-OSIC, while the performance discrepancy becomes smaller as the number of receive antennas increases.

노인복지회관의 공간구성현황에 관한 연구

이효원,박산돌,주석중 한국의료복지시설학회 2004 의료·복지 건축 Vol.10 No.2

This study attempts to provide the fundamental materials for space program in terms of planning a senior welfare center by means of examining and analyzing the present conditions of spatial arrangement of each section in senior welfare center for the aged. The results of the study are as in the following. 1) The demand for new service programs corresponding to the improved quality of the elderly and various social desires is relatively on the increase. Each program in senior welfare center for the elderly should be connected in an organic manner and the organization of space should be provided. 2) The section of counseling, the first step when using a welfare center, should be located in the entrance of the building and it has to be closely connected with other section. 3) The section of medical rehabilitation is a place mostly for the elderly in their frail physical condition; therefore, it is effective to use horizontal line. 4) It is important for the room for volunteers in the section for domiciliary welfare to be arranged flexibly with the office or the room for social education, and it is necessary to consider how easy daytime or short-term care is accessible. 5) The space where dynamic programs are progressed in the section of social education should be distinguished from the section of medical rehabilitation or the room for daytime care and the room for short-term care of the section of domiciliary welfare.

샤또브리앙과 낭만주의적 역사

이산호 중앙대학교 인문과학연구소 2001 人文學硏究 Vol.32 No.-

C.V.D. 방법에 의한 Cd_0.78Zn_0.22 S박막 성장과 광전기적 특성연구

유상하,이상렬,홍광준,서상석,김혜숙,전승룡,윤은희,문종대 조선대학교 기초과학연구소 1993 自然科學硏究 Vol.16 No.1

We had grown Cd_0.78Zn_0.22S polycrystal thin films on slide substrate using chemical vapour deposition(CVD) method. We measured X-ray diffraction patterns in order to study Cd_0.78Zn_0.22S polycrystal structure. We studied its band gap using transmission curves and photocurrent and also analyzed photoluminescence using configurational coordinate model. We measured Hall effect·on this sample by Van der Pauw method and studied on carrier density and mobility depending on temperature.

난자의 회수시기, BSA, Cysteine 및 Myoinositol이 개 난자의 체외성숙에 미치는 영향

김상근,이봉구,이규승 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

This study was carried out to investigate the effects of ovarian estrus stage of oocytes and supplementation of medium with BSA, cysteine and myoinositol on in vitro maturation of canine oocytes. The in vitro maturation(IVM) rate to metaphase II (M II) stage of oocytes recovered from different stage of the reproductive cycle (inactive, follicular and luteal stage) cultured in TCM-199 media were 0.0±0.0%, 10.0±4.1% and 5.7±1.6%, respectively. The IVM rate of oocytes collected from follicular stage was significantly higher in inactive or luteal stage of oocytes. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 0.1 mM cysteine were 0.0+0.0%, 15.8+4.7%, 5.6+1.5%, respectively. The IVM rates of oocytes recovered from different stage of the reproductive cycle cultured in TCM-199 media with 5% BSA and 10 mM myoinositol were 0.0±0.0%, 18.4±4.6% and 5.7±1.9%, respectively. The IVM rate of follicular stage oocytes was significantly higher in oocytes collected from follicular stage and with cultured 5% BSA and 0.1 mM cysteine or 5% BSA and 10 mM myoinositol compared to other experimental group.