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( Yanyan Wu ),( Yongkai Ma ),( Laihao Li ),( Xianqing Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1
An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structure-activity relationship in these peptides. To prepare antioxidant peptides better and improve their stability, the design and expression of an antioxidant peptide from Pinctada fucata (named DSAOP) was studied. The peptide contains a common precursor of an expression vector containing an α-helix tandemly linked according to the BamHI restriction sites. The DNA fragments encoding DSAOP were synthesized and subcloned into the expression vector pET-30a (+), and the peptide was expressed mostly as soluble protein in recombinant Escherichia coli. Meanwhile, the DPPH radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging activity of DSAOP IC50 values were 0.136 ± 0.006, 0.625 ± 0.025, and 0.306 ± 0.015 mg/ml, respectively, with 2-fold higher DPPH radical scavenging activity compared with chemosynthesized AOP (p < 0.05), as well as higher superoxide radical scavenging activity compared with natural AOP (p < 0.05). This preparation method was at the international advanced level. Furthermore, pilot-scale production results showed that DSAOP was expressed successfully in fermenter cultures, which indicated that the design strategy and expression methods would be useful for obtaining substantial amounts of stable peptides at low costs. These results showed that DSAOP produced with recombinant Escherichia coli could be useful in cosmetic skin care products, health foods, and pharmaceuticals.
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Purification and Characteristics of Serine Protease from the Head of Pacific White Shrimp
Yanyan Wu,Ping Wang,Laihao Li,Xianqing Yang,Shiqiang Diao 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.4
Serine protease from the head of Pacific white shrimp was purified by the following techniques: ammonium sulfate fractionation, Q-Sepharose HP ion exchange chromatography, and Sephadex G-100 gel filtration. The molecular weight was estimated as 32.8 kDa using SDSPAGE. The optimum pH and temperature of the enzyme for the hydrolysis of casein were determined to be 10.0 and 40oC. It was stable at pH range from 8.0 to 11.0 and had good thermal stability. Pb2+, Ca2+, Mg2+, Cu2+, and Mn2+could active the enzyme certainly when Zn2+and Hg2+strongly inhibited the activity. The enzyme was inhibited by the general serine protease inhibitor (PMSF) and the specific trypsin inhibitors (TLCK, SBTI). The modification of various amino acid modifiers for the purified enzyme determined that the enzyme active center included tryptophan,histidine, and serine, moreover, arginine had a certain relationship with the enzyme activity.
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Anti-Corrosion Coatings for Protecting Nb-Based Alloys Exposed to Oxidation Environments: A Review
Yingyi Zhang,Tao Fu,Laihao Yu,Kunkun Cui,Jie Wang,Fuqiang Shen,Xu Zhang,Kaichuang Zhou 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.1
Nb-based alloys are regarded as a vital high temperature structural material. Unfortunately, the low oxidation resistance ofthe alloys limits their application under aerobic conditions at high temperature. Surface coating technology is considered tobe an ideal method to solve this problem. This paper reviewed recent progress on silicide coatings on Nb and Nb-based alloy,and focused on different types of modification techniques. The microstructure, phase composition and oxidation propertiesof various silicified coatings are analyzed. The effects of modified elements and second phase on the oxidation mechanismof silicide coatings are also summarized. Finally, the existing problems and future development direction of various silicidecoatings are pointed out.
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