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피임제(Norinyl)가 흰쥐 난소의 Alkaline Phosphatase 활성에 미치는 영향
신경숙,이군자,정호삼,이규식 대한해부학회 1985 Anatomy & Cell Biology Vol.18 No.1
In mammals, female sexual hormones secrete at different rate during the sexual cycle so that the follicular cells and the granulosa cells undergo structural and functional changes. Therefore the authors undertook to pursue the changes of the graafian follicle during the estrous cycle and the effect of the oral-contraceptive. Female Sprague-Dawley rats weighing 150g were used as a experimental animal. Vaginal smears were obtained daily and only animals that showed consecutive four-day estrous cycle were used. The animals of the oral-contraceptive administered groups were administered norinyl, dissolved in distilled-water. The ovary was fixed with 10% neutral formalin and sliced at 14 ㎛ thickness in a frozen cryostat. The activity of alkaline phosphatase was observed by the Gomori's method for histochemical study. The other ovary was homogenized and the homogenate was assayed by the Lowry's method for biochemical study. The results were as follows. 1) In the follicle alkaline phosphatase activity was positive in the theca layer while the granulosa layer was negative. Strong activity were shown at proestrous and metestrous stage during the estrous cycle. And alkaline phosphatase activity of the ovary did not change markedly during the estrous cycle but the activity of estrous stage were slightly higher than other stages. 2) In the norinyl-administered group, the alkaline phosphatase activity of the ovary did not change during the estrous cycle. And the activity of the administered groups was lower than the control group.
Yong Za Chung,Goon Jae Cho,Hyun Ok Jung,Su Kyung Choi,Jung Sook Kang,Il Yun 생화학분자생물학회 1993 BMB Reports Vol.26 No.5
The effects of n-alkanols on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene (DPH) were investigated in the liposomes of total lipids (SPMVTL) extracted from synaptosomal plasma membrane vesicles. n-Alkanols decreased the rotational relaxation time of DPH in the SPMVTL, and the potencies of n-alkanols up to 1-nonanol increased by 1 order of magnitude as the carbon chain length increased by two carbon atoms. The cut-off phenomenon was reached at 1-decanol, where further increase in hydrocarbon length resulted in an increase in the rotational relaxation time of DPH.