Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

( Jae-sung Ryu ),( Kyu-tae Chang ),( Ju-taek Lee ),( Malg-um Lim ),( Hyun-ki Min ),( Yoon-ju Na ),( Su-bin Lee ),( Gislain Moussavou ),( Sun-uk Kim ),( Ji-su Kim ),( Kinarm Ko ),( Kisung Ko ),( Kyung- 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.6

The ACKNOWLEDGEMENTS should be corrected as follows, "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea. The published article in this journal was a part of the 2013 Doctoral thesis of Jae-Sung Ryu submitted to Wonkwang University, Korea." and not "This research was supported by a grant (Code# PJ0074922012) from the Korean Rural Development Administration, and has been supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education Science and Technology (2010-0022316 and KCG5401011), and the KRIBB/KRCF Research Initiative Program (NAP), Korea."

Open Posterior Approach versus Arthroscopic Suture Fixation for Displaced Posterior Cruciate Ligament Avulsion Fractures: Systematic Review

Jae­Gwang Song,Kyung Wook Nha,이세원 대한슬관절학회 2018 대한슬관절학회지 Vol.30 No.4

Purpose: To compare the clinical outcomes between the open posterior approach and arthroscopic suture fixation for displaced posterior cruciate ligament (PCL) avulsion fractures.Methods: A literature search was performed on MEDLINE, EMBASE, and the Cochrane Library databases. The inclusion criteria were as follows: papers written in English on displaced PCL avulsion fractures, clinical trial(s) with clear description of surgical technique, adult subjects, a follow­up longer than 12 months and modified Coleman methodology score (CMS) more than 60 points.Results: Twelve studies were included with a mean CMS value of 72.4 (standard deviation, 7.6). Overall, 134 patients underwent the open posterior approach with a minimum 12­month follow­up, and 174 patients underwent arthroscopic suture fixation. At final follow­up, the range of Lysholm score was 85–100 for the open approach and 80–100 for the arthroscopic approach. Patients who were rated as normal or nearly normal in the International Knee Documentation Committee subjective knee assessment were 92%–100% for the open approach and 90%–100% for the arthroscopic approach. The range of side­to­side difference was 0–5 mm for both approaches.Conclusions: Both arthroscopic and open methods for the treatment of PCL tibial­side avulsion injuries resulted in comparably good clinical outcomes, radiological healing, and stable knees.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

초등학생의 부모애착, 또래애착, 자기효능감, 학교적응 간의 관계 구조분석

이재인 ( Jae In Lee ),김춘경 ( Choon Kyung Kim ),한은수 ( Eun Su Han ) 한국초등교육학회 2014 초등교육연구 Vol.27 No.4

본 연구는 초등학생의 자기효능감, 또래애착, 부모애착이 어떤 경로를 통해 초등학생의 학교 적응에 영향을 미치는지 알아보고자 하였으며 이러한 목적을 달성하기 위해 대구·경북에 거주하는 초등학교에 재학중인 4-6학년 학생을 대상으로 설문지를 이용하여 수집된 자료를 분석하였다. 분석을 위하여 각각의 잠재변인들이 아동의 학교적응에 영향을 미치는 직접적인 경로와 간접적인 경로를 설정한 가설적 연구모델을 설정하였고, 구조방정식모델을 통해 가설적 연구모델이 실제자료와 일치하는지를 분석하였다. 분석결과 초등학생의 학교적응에 직접적인 영향을 주는 요인은 자기효능감과 또래애착이고 부모애착은 초등학생의 학교적응에 직접적인 영향을 미치지 않는 것으로 나타났다. 좀 더 구체적으로 살펴보면 부모애착이 아동의 학교적응에 미치는 직접적인 영향은 없는 것으로 나타났으나 부모애착이 자기효능감과 또래애착을 통하여 학교적응에 미치는 간접효과가 유의하다는 결과를 얻게 되었으며 또래애착과 부모애착은 자기효능감에 직접적인 영향을 미치고 부모애착은 또래애착에 직접적인 영향을 미치는 것으로 나타났다. 마지막으로 초등학교 현장에서 학교적응을 위한 개입방법과 후속 연구를 위한 제언을 제시하였다. The purpose of this study is to conduct an investigation for the structural relationship among parental attachment, peer attachment, self-efficacy and school adjustment perceived by elementary school students. To analysis the structural relationship of the variables related with the school adjustment, we collected a data from the 4th ~ 6th grade students staying in Dae-gu and Kyung-buk using questionnaires. The responses of 567 students for Parental Attachment Scale, Peer Attachment Scale, Self-Efficacy Scale and School Adjustment Scale are used to the final analysis. A variance-covariance matrix of this sample was analyzed using AMOS 18.0 with the maximum likelihood minimization function. The goodness of fit was evaluated via SRMR, RMSEA, with a 90% confidence interval, CFI, TLI. In conclusion, it was confirmed that the peer attachment and self-efficacy by elementary school students are statistically significant on school adjustment. And parental attachment had indirect effect on school adjustment through peer attachment and self-efficacy. Therefore, it is important to develop the peer attachment and self-efficacy enhancement program for children`s school adjustment.

경기도 지역에너지계획의 도민참여 과정 : 민주주의 학습 효과를 중심으로

고재경(Jae-Kyung Koh),진상현(Sang-Hyeon Jin) 한국NGO학회 2021 NGO연구 Vol.16 No.2

분산형 에너지의 확대로 인해 지역의 에너지정책 및 계획 과정에서 시민참여의 필요성에 대한 사회적 인식이 높아지고 있으며, 실제로 시민참여형 에너지계획을 수립하는 사례도 늘어나고 있다. 특히 문재인 정부는 법정 계획인 지역에너지계획을 모든 광역지자체들이 시민참여방식으로 수립하도록 요구하였다. 이에 본 논문은 다른 지자체와 차별화된 방식으로 진행되었던 경기도의 도민참여형 지역에너지계획 수립 과정을 민주주의의 학습이라는 측면에서 분석하였다. 또한 민주주의 학습효과의 산물인 에너지 시민성 관점에서의 평가도 이루어졌다. 연구결과 경기도 사례는 일반 시민의 대표성에 초점을 맞추었던 여타 지자체들과 달리 에너지 소양을 갖춘 시민들이 참여하는 모델을 구축함으로써, 방관자적 시민이 아니라 행동하는 에너지 시민의 양성을 통해 계획의 실행력까지 담보해 낼 수 있었다. 즉, 계획의 수립 과정을 풀뿌리 민주주의의 학습 공간으로 조성한 덕분에 에너지 정책 관련 정보와 지식을 습득한 실천적 시민들이 지역에너지계획의 이행 주체로 성장할 수 있게 되었다. 특히 도민추진단의 학습효과가 계획수립 이후의 에너지전환 활동으로 연결될 수 있도록 참여의 과정과 내용이 설계되었을 뿐만 아니라, 광역과 기초지자체 간의 계획 연계를 통한 정책협력의 토대가 만들어졌다. 사회적 학습 효과를 토대로 시민참여의 성패를 평가해야 한다는 관점에서, 경기도 지역에너지계획의 사례는 의미 있는 성과를 거둔 것으로 보이며 다른 지역의 사례와 차별화된 특징을 지닌 것으로 판단된다. Citizen’s awareness of the importance of participating in the process of local energy policy has increased owing to the expansion of decentralized energy. Consequentially, the cases of residents’ participatory energy planning in the municipalities had frequently been identified. Particularly, the current government of Moon Jae-in asked the whole regional local governments to establish their energy plan in the way of citizen’s participation. In this context, this study attempts to analyze the planning process of Gyeonggi province which adopted its unique participatory method from the viewpoint of learning effect of democracy. In addition, the concept of energy citizenship was adopted to evaluate the overall participation process. Gyeonggi province, unlike other regions that focused on the representativeness of ordinary citizens, built a planning model in which citizens with energy knowledge participate as local representatives, thereby cultivating active energy citizens not the bystanders, and ensured even the execution ability of the plan. In other words, since the participation process was considered as a learning space for grassroots democracy, practical citizens who acquired information and knowledge related to energy policy could grow as the implementing agent of the local energy plan. Additionally the participation process and contents were designed so that their learning effect can be developed into the energy transformation activities after the establishment of the plan. Also Gyeonggi province attempted to establish the linkage between the regional local government and primary local governments to strengthen the policy cooperation. Based on the argument that the success of citizen participation should be evaluated by the effect of facilitating social learning, the case of Gyeonggi-do appears to have achieved meaningful results and has distinctive characteristics from cases in other regions.

볏짚 펄프化에 關한 硏究

金在慶 진주산업대학교 1981 論文集 Vol.19 No.-

本 試驗은 稻葉稈을 供試原料로 하여 짚 펄프化의 適性을 究明하기 위하여 活性 알카리 6, 8, 10 및 12%에서 짚 펄프化 特性을 調査하였으며 그 結果를 要約하면 다음과 같다. 1. 活性 알카리 濃度의 增加에 따라 펄프의 收率이 減少하였다. 2. 펄프 總收率은 來敬이 收率이 가장 높았고 다음은 密陽 30號, 水原 264號의 順이였다. This experiment was carried to determine the quality of their straw pulping characteristics with rice straw in 6, 8, 10 and 12% active alkali solution. The results are summarized as follows: 1. The thicker the concentration of active alkali solution becames, the less the total yield was shown. 2. In the total yield, that of Rae Kyung was shown the highest of the testing materials. 3. The order of the total yield was investigated Rae Kyung, No.30. Milyang, No.264 Suweon.

燃料林造成에 關한 硏究 : 第2報 : 農山村農家一戶當 燃料林(아까시아나무)所要面積에 關한 調査硏究

金是璟,金在慶 진주산업대학교 1976 論文集 Vol.14 No.-

效果的인 燃料林造成을 爲한 適定燃料林 所要面積을 調査코자 1975年 2月∼1976年 5月까지 木校演習林(慶南 山淸郡 今西面)中 点生하고 있는 旣成燃料林分(아까시아)을 對象으로 하여 調査한바 그 結果를 要約하면 다음과 같다. 1). 8年生 根株에서 最大 및 最小萌芽本數는 各各 19本 및 5本이 發生되었으며, ha當 總本數는 28,280本으로 材積은 3.723㎥(2.98ton)로 調査되었다. 2). 5年生 根株에서 生立된 單幹狀의 本數는 ha當 2,890本으로 總材積은 15.895㎥(12.72ton)로서 年間 平均材積成長量은 3.974㎥(3.18ton)로 調査되었다. 3). 本調査에서는 燃料林(아까시아나무의 ha當 平均材積成長量이 3.974㎥(3.18ton)에 불과하나 合理的인 燃料林經營을 한다면 2∼3倍의 增收가 確實하며 또한 林産副産物 및 農業副産物을 燃料로 使用할 수 있으므로 農家一戶當 1ha의 燃料林을 造成하여 4年을 伐期로 連進帶狀皆伐法에 의해 萌芽更新을 實施한다면 農山村의 所要燃料量을 充當할 수 있을것으로 思料된다. This study was carried out to make the basic investigation fot the establishment of the fuel wood land from Feb. 1973 to May 1974. It was attempted to investigate the year fuel consumption for a standard farm house at the mountain village, farm land village, and the suburban farm village, and also to investigate the requirement of appropriate area of the fuel wood land, and it was investigated at the stand of Robinia pseudoacacia L. scattered in college forest located San Cheong, Kyung Nam. The results were summarized as follows. 1). The number of the maximum and minimum 8 year's sprouts which were grown at the root stock of Robinia pseudoacacia L. was 19 trees and 5 trees each. The number of the total tree per one hectare were 28,28O trees, and the volume of those were 3.723㎥(2.98tons). 2). The number of one stem form tree grown 5year's root stock of Robinia pseudoacacia L. was 2,980 trees, per one hectare. The total volume of the those was 15,895㎥(12.72tons), and the average year growth of volume was 3,974㎥(3.2tons). 3). The average year growth of Robinia pseudoacacia L. will be increased more than twice or three times comparing to the above through the more effective management of the fuel wood 1and, and when the four year term for final cutting are fol1owed the sprout reproduction by the progressive clear cutting in strip, and the by-products of agriculture and forest are used for the fuel, One hectare of the fuel wood land will be enough to the average year fuel consumption needed for the mountain and farm land village.

신이식후 발생하는 감염에 대한 고찰

김재필(Jae Phil Kim),조대옥(Dae Ok Cho),고경식(Kyung Sik Ko),안재형(Jai Hyung Ahn),이태원(Tae Won Lee),임천규(Chun Gyoo Ihm),김명재(Myung Jae Kim) 대한내과학회 1995 대한내과학회지 Vol.49 No.1

N/A Objectives: Renal transplantation is a major therapeutic advance for patients with chronic renal failure. But recipients of renal transplantation are prone to infection with both common and unusual organisms. And infectious diseases remain a major cause of morbidity and mortality in renal transplant recipients. This study was to analyze the infections in renal transplant recipients; its occurrence according to sites and organisms; comparison among immunosuppressive agents; prognostic differences of urinary tract infections(UTI) between those developed during 1 month after transplantation and those not developed; graft outcome; and patients mortality. Mothods: 181 renal transplant recipients were examined. They received renal transplantion between january 1979 and December 1992 at the Kyung-Hee University Hospital. 158 of them received transplantation from living-related donors, 22 from living-unrelated donors, and 1 from cadaver donor. Their age at the time of transplantation ranged from 14 to 66 years(mean 35.6 years), and the male to female ratio was 2.3: 1. The observation period ranged from 1 to 144 months(41.26±31.71, mean±S. D.). Results: 1) 110 recipients(60.7% of total) had 232 episodes of infectious complications whereas 71(39.3% of total) had no infectious episodes. About half of infectious episodes(128 episodes, 55.2% ) occurred during 1 month after transplantation. 2) The most common site of infection was urinary tract(138 episodes, 59.2%) whth the next sites coming in this order, bacteremia(32 episodes, 13.8%), pulmonary(21 episodes, 9.1%), and skin(21 episodes, 9.1%). 3) The causative organisms of UTI ranked in this order E. coli 21.7%, staphylococcus spp 14.5%, and pseudomonas spp 13.0%. 4) The causative organisms of bacteremia ranked in this order E. coli 34.4% salmonella spp 18.8%, pseudomonas spp 12.5 %, and staphylococcus spp 12.5%. 5) There was no significant differences of infectious episodes among immunosuppressive regimens. 6) Early UTI group(UTI occurred during 1 month after transplantation) recorded significantly higher incidence of urinary tract infections after 1 month that followed than non-UTI group. But there was no differences between two groups on graft and patients outcome. 7) The major cause of death was life-threatening infections(63.2 %). Conclusion: Most infections due to various organisms may occur during 1 month after transplantation. And still they constitute a major cause of death in renal transplant recipients.

한강개발이전 한강하구와 경기만에서의 퇴적환경

오재경(Jae-Kyung OH) 인하대학교 해양과학기술연구소 1989 黃海硏究 Vol.2 No.5