‘신시론’동인의 역할과 위치 연구

권경아(Kyung-a, Kwon) 어문연구학회 2009 어문연구 Vol.62 No.-

The Sinshiron coterie was formed to go beyond the achievements and limits of Korean modernist poetry of the 1930s and sought a new world of poetry in response to the post-liberation changing environment. They published their first poetry magazine Sinshiron in 1948 and their second coterie magazine A New City and the Chorus of Citizens in 1949. However, the Sinshiron has not been discovered until recently, and thus the previous studies on Sinshiron have mainly relied on the latter magazine. Most of those studies regard ‘Sinshiron’ as a modernist coterie and view the coterie as predecessor or prototype of the ‘Hubangi’ coterie, with emphasis on the facts that the magazine A New City and the Chorus of Citizens was a modernism-oriented coterie magazine and that Kim Kyung-rin and Park In-hwan who organized and led the ‘Sinshiron’ coterie claimed to stand for modernism. Now, however, such a point of view requires modification after the first issue of Sinshiron was recently found. Actually, the magazine contains a large number of poems that stand for engaged realism and the coterie members also appear to have had a realistic tendency. In fact, during its early stage, the Sinshiron coterie had a membership of both realist and modernist writers, but it was later disorganized due to increased ideologicalconflict between two groups. After that, the ‘Hubangi’ coterie was formed by Kim Kyung-rin and Park In-hwan, who played a leading role in the Korean modernism of the 1950s. In Korean modernism, the ‘Sinshiron’ coterie's group-based poetry movement allows us to get an overall view of modernist poetry activities of the 1930s and 1950s and has a considerable significance in that they greatly influenced the modernism of the 1950s.

A형 간염의 자연항체와 예방접종을 통한 항체 생성률의 역가 비교분석

권원현,김경화,조경아,문기춘,김정인,이인원,Kwon, Won Hyun,Kim, Kyung Hwa,Cho, Kyung A,Moon, Ki Choon,Kim, Jung In,Lee, In Won 대한핵의학기술학회 2013 핵의학 기술 Vol.17 No.2

2008년부터 A형 간염 환자들이 급속히 증가하고 본원에 내원하여 건진을 받는 대부분의 수검자들이 A형 간염(IgG) 항체 생성 유무에 관심이 많아지며 검사 건수가 증가하였다. 그에 따라 항체 검사결과가 cut-off값에 걸리는 검체가 많아져 원인을 분석하였더니 대부분 A형 간염 예방접종을 한 수검자들이었다. 이에 저자들은 건강증진센터에서 설문조사를 통하여 자연면역을 획득한 수검자들 그룹과 본원에서 A형 간염 예방접종(1차, 2차)을 실시한 직원들 그룹으로 나누어 검사를 시행하였고 cut-off값을 기준으로 항체 생성률과 그에 대한 역가를 비교하고 진단검사의학과와 핵의학과에서 사용하는 진단 시약간에 항체 생성률과 그에 대한 역가를 비교해 보고자 했고, 2012년 8월 한 달 동안 건진 수검자 185명을 설문조사하여 자연면역을 획득한 119명과 본원에서 예방 접종을 실시한 직원들을 대상으로 1차 접종자 53명, 2차 접종자 59명으로 대상을 분류했다. 항체 생성률은 cut-off값 1을 기준했을 때 0.90-1.10 (${\pm}$), 0.60-0.89 (1+), 0.30-0.59 (2+), 0.01-0.29 (3+)로 나누어 역가를 비교하고, 같은 기준으로 제조사별 백신 접종 후 항체 생성률에 대한 역가를 비교평가 해 보았다. 그 결과, 건진 수검자 중 자연 면역을 획득한 수검자는 cut-off값 1을 기준했을 때, 0.90-1.10 (${\pm}$)가 0%, 0.60-0.89 (1+)가 0%, 0.30-0.59 (2+)가 4.2%, 0.01-0.29 (3+)가 96%로 역가가 <0.60 ($${\geq_-}2+$$)가 100%였다. 그리고 예방접종을 실시한 직원들의 항체 생성률은 1차 접종자 중 ${\pm}$가 59.1%, 1+가 18.1%, 2+가 18.1%, 3+가 4.6%로 총 45.3%였고, 역가는 $${\geq_-}$$ 0.60 ($${\leq_-}1+$$)가 77.3%였다. 2차 접종자의 항체 생성률은 ${\pm}$가 1.9%, 1+가 15.4%, 2+가 36.54%, 3+가 46.2%로 총 88.1%였고 역가는 <0.60 ($${\geq_-}2+$$) 82.7%가 였다. 또한 제조사별로 비교 하였을 때 1차 접종자의 항체 생성률은 BNIBT 20.8% (${\pm}24.5%$), GB 15.7% (${\pm}7.8%$), RIAKEY 94.3% (${\pm}3.8%$), ROCHE 83% (${\pm}0%$), Abbot 73.1% (${\pm}5.8%$)였고, 2차 접종자의 항체 생성률은 BNIBT 86.4% (${\pm}1.7%$), GB 88.5% (${\pm}1.9%$), RIAKEY 100% (${\pm}0%$), ROCHE 98.3% (${\pm}0%$), Abbot 98.2% (${\pm}0%$)였다. 즉 자연면역 항체가 예방접종에 의한 항체보다 역가가 높다는 것을 알 수 있었고, 1차 접종 후 보다는 2차 접종 후 검사를 시행했을 때 항체 생성률과 역가가 대부분 높아짐을 알 수 있었다. 따라서 결과 보고시 negative, index (${\pm}$), weak positive (1+), positive (2+), strong positive (3+)로 역가를 나누어 보고를 하거나 결과값에 index값을 같이 적어서 결과를 상세히 보고한다면 과거결과와 비교도 가능할 것이다. 또 제조사별 비교 시 1차 예방접종 후의 항체 생성률과 역가에서 시약간에 많은 차이를 보이고 있었고, 매년 예방 접종률이 높아지고 있는 시점에서 이러한 차이를 줄이기 위해서 각 제조사들은 민감도나 재현성에 더 주의를 기울여야 하겠고, 자연면역항체와 예방접종을 통한 항체간에 생길 수 있는 미지의 차이를 감안하여 검사자들이 사용하는 시약을 신뢰할 수 있도록 더 연구하고 개발해야 할 것이다. Purpose: Since 2008, hepatitis A patients was rapidly increasing. So, Most of the health checkup examinees were interested in whether hepatitis A antibody was a lot. thereby The number of tests was increasing. In recent years, Antibody test results in the range of cut-off values were increased. According to the cause analysis, most examinees had a hepatitis A vaccine. This study was conducted to classify hepatitis A antibody as natural antibody and antibody after vaccination and compared the titer for seroconversion rate based on cut-off values. Materials and Methods: For a month in August 2012, First, We surveyed 185 health examinees and classified 119 health examinees who had acquired natural antibody. Second, for employees who were inoculated against hepatitis at our hospital, We classified into 53 primary inoculators and 59 secondary inculators. when the standard of cut-off value was 1, The seroconversion rate was compared the titer divided by 0.90-1.10 (${\pm}$), 0.60-0,89 (1+), 0.30-0.59 (2+), 0.01-0.29 (3+) and we compared the titer for seroconversion rate by each manufacturer after vaccination. Results: When the standard of cut-off value was 1, the titer of 119 health examinees who had acquired natural antibody was 0.90-1.10 (${\pm}$): 0%, 0.60-0.89 (1+): 0%, 0.30-0.59 (2+): 4.2%, 0.01-0.29 (3+): 96% and the titer of <0.60 ($${\geq_-}2+$$) was 100%. The titer of 53 primary inoculators was 0.90-1.10 (${\pm}:59.1%$), 0.60-0.89 (1+): 18.1%, 0.30-0.59 (2+): 18.1%, 0.01-0.29 (3+): 4.6% and the seroconversion rate was 45.3%. The titer of $${\geq_-}0.60$$ ($${\leq_-}1+$$) was 77.3%. The titer of 59 secondary inoculators was 0.90-1.10 (${\pm}:1.9%$), 0.60-0.89 (1+): 15.4%, 0.30-0.59 (2+): 36.54%, 0.01-0.29 (3+): 46.2% and the seroconversion rate was 88.1%. The titer of <0.60 ($${\geq_-}2+$$) was 82.7%. When we compared the titer for seroconversion rate by each manufacturer after vaccination, the seroconversion rate of 53 primary inoculators was BNIBT: 20.8% (${\pm}:24.5%$), GB: 15.7% (${\pm}:7.8%$), RIAKEY: 94.3% (${\pm}:3.8%$), ROCHE: 83% (${\pm}:0%$), ABBOTT: 73.1% (${\pm}:5.8%$) and the seroconversion rate of 59 secondary inoculators was BNIBT : 86.4% (${\pm}:1.7%$), GB: 88.5% (${\pm}:1.9%$), RIAKEY: 100% (${\pm}:0%$), ROCHE: 98.3% (${\pm}:0%$), ABBOTT: 98.2% (${\pm}:0%$). Conclusion: The study show that the titer of natural immune antibodies is higher than the titer of vaccination and the titer of secondary inoculation is mainly higher than the titer of primary inoculation. Consequently, if we know the titer of hepatitis A antibodies, it will help to give resullt reports. And then, when we compared the titer and the seroconversion rate by each manufacturer, There was a very distinct difference. As the test subjects inoculate against hepatitis A (HAV), it is considered BNIBT, GB will occur false negative rate and RIAKEY, ROCHE, ABOTT will occur false positive rate.

패션 상권(商圈)에 따른 소비자(消費者)의 패션라이프 스타일과 의복구매유형분석(衣服購買類型分析)

김경아 ( Kyung A Kim ),유태순 ( Tai Soon Yoo ) 한국패션비즈니스학회 1999 패션 비즈니스 Vol.3 No.3

This study is to analyze consumer`s visiting fashion trade area. The purpose of this study, focused on Taegu fashion area, Dongsungro and Bum udong, consumer`s spatical beravior, fashion- life-style, clothing purchasing type and Store- Image, etc. This study targeted 580 women aged from 15 to 25 visiting fashion trade area. The methods of measuring include consumer behavior scale, fashion life style scale, clothing purchasing type scale and Stor - Image scale. In process of statistics, the frequency was used to measure consumer`s spatical behavior, MANOVA to measure, fashion life style and X2 examination was employed to measure clothing purchasing type. The followings are the conclusions of this study ; 1. consumer`s spatical behavior by FTA(fashion trade area) : Most people visiting Dongsungro A trade area and Bum udong trade area depart home, while Dongsungro B trade area from school. In case of Bum udong trade area, in particular, more people move from other trade area than people do in Dongsungro trade area. 2. Consumer spatical behavior means by FTA : Bus is far most common transportation. 3. The purpose of visit by FTA : Most people visit Dongsungro B trade area for shopping, while Bum udong trade area for shopping and as a place for appointment. 4. The visiting frequency by FTA : In case of both Dongsungro A.B trade area and Bum udong trade area, at least once a week is most common. Particularly in case of Bum udong trade area, the number of people visiting once every three months and once more than six months is almost same. 5. A company by FTA : People accompanied by the same sex friends are most common in Both Dongsungro A, B trade area and Bum udong trade area in case of Dongsungro B trade area. There is no case of people accompanied by family. 6. The purpose of purchasing fashion comodities by FTA : In case of Dongsungro A trade area for recreation. In case of Dongsungro B trade area and Bum udong trade area, because of affordable price. 7. A Brand - pursued tendency by FTA : Much higher in Dongsungro trade area than Bum udong trade area, among consumers. A character - pursued tendency by FTA : Higher in Dongsungro A than Dongsungro B,A practical tendency and symphatetic tendency Higher in Dongsungro B than Dongsungro A or Bum udong trade area. 8. A Store - Image scale by FTA : The quality of goods is more important to consumers in Dongsungro B than to consumers in Dongsungro A. The data - service and atmosphere are much more important to consumers in Dongsungro B than to consumers in Dongsungro A and Bum udong trade area. The convenience is more important to consumers in Dongsungro B Bum udong trade area than to consumers in Dongsungro A. 9. There is no significant difference among clothing purchasing types by FTA.

The Role of Adenosine Receptors on Acetylcholine Release in the Rat Striatum

Do Kyung Kim,Hyeon A Kim,Bong Kyu Choi 대한생리학회-대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.1

<P> As it has been reported that the depolarization induced acetylcholine (ACh) release is modulated by activation of presynaptic A<SUB>1</SUB> adenosine heteroreceptor and various evidence suggest that indicate the A<SUB>2</SUB> adenosine receptor is present in the striatum, this study was undertaken to delineate the role of adenosine receptors on the striatal ACh release. <P> Slices from the rat striatum were equilibrated with [<SUP>3</SUP>H]choline and then the release amount of the labelled product, [<SUP>3</SUP>H]ACh, which was evoked by electrical stimulation (rectangular pulses, 3 Hz, 2 ms, 24 mA, 5 Vcm<SUP>-1</SUP><SUB>, </SUB>2 min), was measured, and the influence of various agents on the evoked tritium outflow was investigated. And also, quantitative receptor autoradiography and drug-receptor binding assay were performed in order to confirm the presence and characteristics of A<SUB>1</SUB> and A<SUB>2</SUB> adenosine receptors in the rat striatum. <P> Adenosine (10∼100 ㄍM) and N<SUP>6</SUP>-cyclopentyladenosine (CPA, 1∼100 ㄍM) decreased the [<SUP>3</SUP>H]ACh release in a dose-dependent manner without changing the basal rate of release in the rat striatum. The reducing effects of ACh release by adenosine and CPA were abolished by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 2 ㄍM), a selective A<SUB>1</SUB> adenosine receptor antagonist, treatment. The effect of adenosine was potentiated markedly by 3,7-dimethyl-1-propargylxanthine (DMPX, 10 ㄍM), a specific A<SUB>2</SUB> adenosine receptor antagonist. 2-P-(2-carboxyethyl)phenethylamimo-5 -N-ethylcarboxamidoadenosine hydrochloride (CGS-21680C), in concentrations ranging from 0.01 to 10 ㄍM, a recently introduced potent A<SUB>2</SUB> adenosine receptor agonist, increased the [<SUP>3</SUP>H]ACh release in a dose related fashion without changing the basal rate of release. These effects were completely abolished by DMPX (10 ㄍM). In autoradiography experiments, [<SUP>3</SUP>H]2-chloro-N<SUP>6</SUP>-cyclopentyladenosine ([<SUP>3</SUP>H]CCPA) bindings were highly localized in the hippocampus and the cerebral cortex. Additionally, lower levels of binding were found in the striatum. However, [<SUP>3</SUP>H]CGS-21680C bindings were highly localized in the striatal region with the greatest density of binding found in the caudate nucleus and putamen. Lower levels of binding were also found in the nucleus accumbens and olfactory tubercle. In drug-receptor binding assay, binding of [<SUP>3</SUP>H]CCPA to A<SUB>1</SUB> adenosine receptors of rat striatal membranes was inhibited by CPA (K<SUB>i</SUB> = 1.6 nM) and N-ethylcarboxamidoadenosine (NECA, K<SUB>i</SUB> = 12.9 nM), but not by CGS-21680C (K<SUB>i</SUB> = 2609.2 nM) and DMPX (K<SUB>i</SUB> = 19,386 nM). In contrast, [<SUP>3</SUP>H]CGS-21680C binding to A<SUB>2</SUB> adenosine receptors was inhibited by CGS-21680C (K<SUB>i</SUB> = 47.6 nM) and NECA (K<SUB>i</SUB> = 44.9 nM), but not by CPA (K<SUB>i</SUB> = 2099.2 nM) and DPCPX (K<SUB>i</SUB> = 19,207 nM). <P> The results presented here suggest that both types of A<SUB>1</SUB> and A<SUB>2</SUB> adenosine heteroreceptors exist and play an important role in ACh release in the rat striatal cholinergic neurons.

김경린 시에 나타나는 현대성 연구

권경아(Kwon Kyung-a) 가천대학교 아시아문화연구소 2009 아시아문화연구 Vol.17 No.-

The modernist movements of the 1950s in Korea are discussed with focus on the activities of Hubangi coterie members, especially Kim Kyung-rin. Kim Kyung-rin provided an important feature in Korean modernism of the 1950s by continuing to experiment with the modernist style while joining coterie groups from Shin Siron (New Poetics) to Hubangi. His discussion about modernity starts from the inflow of modern civilization represented by machines and materials and from the resulting fear and despair. The Japanese colonial rule and the inflow of modern civilization after the nation's independence from Japan brought fear and despair to people but, at the same time, raised the urgent need to develop a new understanding of modernity. Kim argued that stereotypical ideas should be reformed along with the problems of expression in modern poetry, reacting against traditional poetry, especially the lytic poems of Cheongnok-pa poets. As a result, he placed emphasis on the use of novel poetic words based on a color sensation, the creation of new language by an unconventional combination of languages, the adoption of geometric poetic diction, and the control of extreme feelings. Based on such poetics, his poems reveal a tough of urban senses, a preference of graphic images, and a combination of new languages. It should be indicated that there was a certain limit to his understanding of modernity, but having attempted to present a new understanding of modernity whileleading the modernism of the 1950s, his poems have great significance in that they played a bridge role in the history of modernist poetry running from 1930s to 1950s.

고온환경 하에서 착용하는 인체냉각 보조도구로서의 Cooling Vest 연구(1) : Local Cooling에 따른 인체의 온열생리학적 특성 Thermophysiological Response of Human Body in Local Cooling

권오경,김진아,김태규 한국의류산업학회 2000 한국의류산업학회지 Vol.2 No.3

Heat stress results in fatigue, a decline in strength, alertness., and mental capacity The problem is compounded when high humidity exists. To help relieve worker heat stress, many types of cooling units are marketed. While workers may experience some cooling, critical body core temperatures often continue to elevate. This study was designed to find the effects of three kinds of cooling vest with portable frozen gel strips on thermophysiological parameters and on temperature and humidity within clothing. The heart rate, rectal, and skin temperature as well as sweat rate and clothing microclimate were measured during 80 min in 5 healthy males. Inquiries were also made into the subjective rating thermal, humidity comfort, and fatigue sensations. The main findings in our experiments are as follows: (a) Physiological parameters such as rectal temperature was the lowest in garb A1, intermediate in garb A, and the highest in garb A2 throughout the experiment. And mean skin temperature was the lowest in garb A, intermediate in garb A1, and the highest in garb A2; (b) Temperature and humidity within clothing (back) were garb in Al, intermediate in garb A, and the highest in garb A2. But the temperature and humidity within clothing (chest) were garb in A, intermediate in garb A1, and the highest in garb A2; (c) Most participants (4 out of 5 persons) answered that they felt more comfortable and fatigueless in garb A1 than in garb A and A2. It is concluded that local cooling in garb A1 of the upper torso could physiological reduce the thermal strain in participants wearing cooling vest.

인간 혈장 아포지단백질 A-1 ( apo A-1 ) 에 대한 단일클론 항체 생산 및 단일클론 항체를 사용한 혈중 apo A-1 측정용 효소면역분석법 ( ELISAs ) 의 개발

이동익,남경수,한문희,김태웅,곽주원,윤미청,최성아 한국지질학회 1994 韓國脂質學會誌 Vol.4 No.1

Recently, apolipoprotein(apo) A-I has received considerable clinical attention as a better marker of inverse correlation with the presence of coronary heart disease than the customary lipid marker, high-density lipoprotein(HDL)-cholesterol. Here we report the production of marine monoclonal antibodies(Mabs) designated as MabA 12 and MabA34 against human apo A-I, recognizing the HDL in plasma. From isotyping analyses, it was found that MabA 12 and MabA34 had IgG2b and IgGI isotype, respectively. Both of the Mabs had κ light chains. The binding specificity of two Mabs was analyzed by immunoblotting and enzyme-linked immunosorbent assay(ELISA). MabA12 and MabA34 reacted well with purified apo A-I or HDL in plasma but they didn't react with low-density lipoproteins(LDL), serum albumin or lipoprotein-deficient serum(LPDS). These indicate that the two Mabs are very specific to human apo A-I in HDL. The validity of the Mabs for serum apo A-I assays was examined by two kinds of ELISA, i.e., non-competitive and competitive ELISA. Both assays revealed that MabA12 or MabA34 reacted well with apo A-I in sample in concentration-dependent manners. Standard curves and, antibody titration curves were drawn. In conclusion, two IgG-type monoclonal antibodies specific to human apo A-I in HDL were produced and applicated for ELISAs of serum apo A-I. The two Mabs would be useful not only for research purposes but also for further development of immuno-diagnostic kits for the measurement of serum apo A-I concentrations.

청가시덩굴 추출물의 기능성 원료 표준화를 위한 지표성분 Resveratrol, trans-Scirpusin A의 분석법 개발 및 검증

권진관(Jin Gwan Kwon),정연우(Yeon Woo Jung),최윤혁(Yun-Hyeok Choi),이지은(Ji Eun Lee),정원식(Wonsik Jeong),이정아(Jung A Lee),최춘환(Chun Whan Choi),안은경(Eun-Kyung Ahn),최용문(Yongmun Choi),홍성수(Seong Su Hong) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 HPLC를 이용하여 청가시덩굴 추출물을 개별인정형 건강기능식품의 기능성 원료로 개발하기 위한 원료 표준화의 일환으로, 청가시덩굴 추출물의 지표성분을 resveratrol과 trans-scirpusin A로 설정하고 이에 대한 HPLC 분석법을 확립하여 유효성의 검증을 실시하였다. 분석법 유효성 검증은 특이성, 직선성, 정확도, 정밀도, 검출한계 및 정량한계 등을 통해 분석법의 신뢰성을 검증하였으며, 그 결과 표준용액과 청가시덩굴 추출물 간의 HPLC 크로마토그램 및 UV spectrum의 일치 여부 등의 비교를 통해 다른 물질과 간섭 없이 피크가 분리된 것으로 특이성을 확인하였다. 또한 표준용액 검량선의 상관계수(R²)는 0.9999로 매우 우수한 직선성으로 관찰되어 분석에 적합한 것으로 확인되었으며, 검량선의 기울기 및 표준편차를 이용한 검출한계는 resveratrol이 0.98 μg/mL, trans-scirpusin A는 0.49 μg/mL였고 정량한계는 resveratrol이 2.98 μg/mL, trans-scirpusin A는 1.48 μg/mL로 각각 확인되었다. 청가시덩굴 추출물에 표준물질을 3개 농도 첨가하고 분석한 회수율은 resveratrol이 98.77~99.24%, trans-scirpusin A는 98.45~99.45%로 나타나 정확성이 있는 것을 확인할 수 있었다. 청가시덩굴 추출물의 조제 농도 2.2, 4.4 및 6.6 mg/mL에서 반복성은 resveratrol이 0.99~1.22%, trans-scirpusin A는 1.12~1.32%를, 실험실 내 정밀성에서는 일내 정밀성은 resveratrol이 0.67~0.87%, trans-scirpusin A는 1.18~1.33%로 나타났고 일간 정밀성은 resveratrol이 0.93~1.22%, trans-scirpusin A는 1.33~2.27%로 확인되어 본 분석법은 정밀성이 있음을 확인할 수 있었다. 이상의 분석결과를 통해 확립된 청가시덩굴 추출물의 지표성분인 resveratrol과 trans-scirpusin A의 HPLC 분석법은 적합한 시험법으로 검증되었으며, 본 시험법은 향후 청가시덩굴 추출물의 건강기능식품 기능성 원료 개발과 표준화를 위한 기초자료로 활용될 것으로 사료된다. This study was undertaken to establish an analytical method using high-performance liquid chromatography (HPLC). HPLC for the standard determination of resveratrol and trans-scirpusin A as functional ingredients in Smilax sieboldii extract. We evaluated the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) of various analytical methods for detecting resveratrol and trans-scirpusin A using HPLC. The specificity was confirmed by the chromatogram obtained using the HPLC analytical method. Also, the results of UV and the coefficient of correlation (R²) obtained was 0.999, which confirmed that this was a suitable analysis with high linearity. The LOD was 0.98, 0.49 μg/mL, and LOQ was 2.98, 1.48 μg/mL, which was confirmed as a suitable limit level for the analysis of resveratrol and trans-scirpusin A content in the S. sieboldii extract. The recovery of resveratrol and trans-scirpusin A content was determined to be 98.77±0.73∼99.24±1.47% and 98.45±1.18∼99.45±1.66%, respectively, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.67∼0.87%, 1.18∼1.33% and 0.93∼1.22%, 1.33∼2.27%, respectively, for trans-scirpusin A, for the relative standard deviation. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of resveratrol and trans-scirpusin A in S. sieboldii extract.

자궁 내 감염 관련 유전자 및 산화 스트레스 관련 유전자와 조기분만의 연관성

유시연 ( Si Yeon You ),김정명 ( Jung Myung Kim ),이경아 ( Kyung A Lee ),박미혜 ( Mi Hye Park ),김영주 ( Young Ju Kim ),전선희 ( Sun Hee Chun ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.7

목적: 조기 분만과 자궁 내 감염 관련 유전자 (IFN-γ, interleukin [IL]-10) 및 산화 스트레스 관련 유전자 (CYP1A1)와의 연관성을 알아보고자 한다. 연구 방법: 2007년 1월부터 2008년 12월까지 이대 목동병원 산부인과에서 산전 검사 및 분만을 시행한 산모 중 37주 이전에 분만한 조산군 164명과 37주 이후에 분만한 대조군 305명을 대상으로 하였으며, 임신 주수 24주 미만, 태아의 선천성 기형, 다태아 분만, 사산의 경우를 제외하였다. 참여자는 입원 시 키, 몸무게 측정 및 혈액 채취를 시행하였고 임신 주수는 최종 월경일 또는 초음파 측정을 통해 계산 되었다. 산모의 혈액에서 IFN-γ, IL-10, CYP1A1 유전자형을 PCR과 SNaPShot method를 사용하여 검사하였으며 카이제곱 검정과 다중 로지스틱 회귀분석을 사용하여 통계분석 하였다. 결과: 조기 분만군과 만삭 분만군 사이에 임신 주수, 태아 체중에 통계적으로 유의한 차이가 있었다(P<0.0001). IFN-γ, IL-10, CYP1A1 유전자형의 빈도와 조기 분만 사이에는 유의한 상관성이 없었다(P>0.05). 결론: 본 연구에서 IFN-γ (874A/T) 및 IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C)와 CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) 유전자형의 빈도는 조산과 관련이 없었다. Objective: To investigate the association between preterm birth and cytokine genes (IFN-γ, interleukin (IL)-10) in intrauterine infection and enzyme gene (CYP1A1) in oxidative stress response. Methods: This study involved a case-control study conducted at Ewha Womans University Hospital in Seoul, Korea. Subjects with preterm deliveries (<37 weeks of gestation) and normal controls with term deliveries (≥37 weeks of gestation) were selected from gravidas who had undergone prenatal examinations in the hospital and were followed until infant delivery. The weight, height, and blood samples of each participants were obtained according to standard protocols. We included subjects who gave birth to a singleton infant and had a gestational age between 24 and 42 weeks. Mutiple births, stillbirths, and congenital anomalies were excluded. Finally, 164 gravidas with preterm births and 305 normal controls with term deliveries were enrolled in the present study. Results: Preterm delivery group and term delivery group had significant difference in gestational age and neonatal body weight (P<0.0001). There were no statistically significant association between preterm birth and IFN-γ, IL-10, CYP1A1 genes (P>0.05). Conclusion: In this study, IFN-γ (874A/T), IL-10 (1082A/G), IL-10 (819C/T), IL-10 (592A/C) and CYP1A1 (T6235C), CYP1A1 (Ile462val [A/G]) genes had no significant association with preterm birth.

Synthesis of Novel Apio Carbocyclic Nucleoside Analogues as Selective A_(3) Adenosine Receptor Agonists

Hwang, Ki-Jun,Chun, Moon-Woo,Jacobson, Kenneth A.,Jeong, Lak-Shin,Lee, Jeong-A,Moon, Hyung-Ryong,Kim, Hea-Ok,Kim, Kyung-Ran,Lee, Kang-Man,Kim, Bum-Tae 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16

On the basis of the biological activity of neplanocin A and apio-dideoxyadenosine (apio-ddA), novel apio-neplanocin A analogues 5a- d, combining the properties of two nucleosides, were stereoselectively synthesized. The apio moiety of the target nucleosides 5a-d was stereoselectively introduced by treating· lactol 10 with 37% formaldehyde in the presence of potassium carbonate. The carbasugar moiety of neplanocin A was successively built by exposing diene 12 on a Grubbs catalyst in methylene chloride. The final nucleosides 5a-d were synthesized from the condensation of the glycosyl donor 14 with nucleic bases under the standard Mitsunobu conditions. Similarly, apio-aristeromycin 6 and (N)-apio-methanocarbaadenosine 7 were derived from the common intermediate 13 using catalytic hydrogenation and Simmons-Smith cyclopropanation as key steps. All of the final nucleosides 5a-4, 6, and 7 did not show significant inhibitory activity against S-adenosylllolllocysteine hydrolase (SAH) up to 100 ㎛, maybe due to the absence of the secondary hydroxyl group at tile C3'-position, which should be oxidized by cofactor-bound NAD^(+). However, aplo-neplanocin A (5a) showed potent and highly selective binding affinity (K_(i), = 628 ± 69 nM) at the A_(3) adenosine receptor without any binding affinity at the A_(1) and A_(2A) adenosine receptors. In conclusion, we have first developed novel carbocyclic nucleosides with unnatural apio-carbasugarsusing stereoselective hydroxymethylation and RCM reaction and also discovered a new template of human A_(3) adenosine receptor agonist, which play a great rote in developing new A_(3) adenosine receptor agonist as well as in identifying the binding site of the receptor.