Assessment of Hepatic Steatosis Improvement Using Controlled Attenuation Parameter in Patients with Non-Alcoholic Fatty Liver Disease under Regular Follow Up

( Kyu Sik Jung ),( Jun Yong Park ),( Mi Na Kim ),( Hana Park ),( Yun Bin Lee ),( Joo Ho Lee ),( Yeonjung Ha ),( Beom Kyung Kim ),( Seung Up Kim ),( Sang Hoon Ahn ),( Do Young Kim ),( Hana Park ),( Kyu 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The change of hepatic steatosis in non-alcoholic fatty liver disease (NAFLD) patients under regular follow up has not been widely investigated. We investigated the serial changes of hepatic steatosis assessed by controlled attenuation parameter (CAP) and the predictors for improvement of hepatic steatosis in NAFLD patients. Methods: Among 513 NAFLD patients diagnosed upon ultrasound and CAP > 250 dB/m at baseline, 304 patients underwent repeated CAP measurement at baseline and at follow up. The improvement of hepatic steatosis was defined as a >10% decline of CAP value from the baseline. Results: Mean age of 304 patients was 58.8 ± 11.4 years and 80.1% were male. The mean CAP value significantly decreased from baseline to follow up (292.3 dB/m to 277.1 dB/m, P<0.001). During the median follow up of 15.3 (9.5-20.3) months, 34.5% patients showed improvement of hepatic steatosis. In a univariate analysis, low body mass index (BMI), low weight, low liver stiffness value, low triglyceride level, and low ALT at follow up were predictors for improvement of hepatic steatosis. In multivariate analyses, low weight (P=0.004; hazard ratio [HR],0.965; confidence interval [CI], 0.941-0.988) and low ALT (P=0.004; HR,0.971;CI, 0.951-0.990) at follow up, or low BMI (P=0.006; HR,0.881; CI, 0.805-0.964) and low ALT (P=0.004; HR,0.971; CI, 0.951-0.990) at follow up were the independent predictors. In patients with weight reduction more than 10% (n=22), CAP value significantly decreased (from 288.0 dB/m to 228.9dB/m, P<0.001). Conclusions: Weight reduction, and ALT decrease were independent predictors for improvement of hepatic steatosis. Long term effect of weight reduction on improvement of hepatic steatosis and fibrosis should be investigated further.

Uncoupling Protein 3의 골격근 세포내 과발현이 OLETF 백서 및 배양된 골격근 세포에서 포도당대사에 미치는 영향

한정희,박혜선,고정민,김하영,강호경,이인규,박중열,홍성관,이재담,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:Uncoupling protein(UCP)는 미토콘드리아의 내막에 위치하는 단백질로 세포내의 과다한 에너지를 열로 발산시키는 기능을 가진다. 최근 동물의 갈색지방조직에만 존재하는 UCP와 유사성을 가진 아형들(UCP2,3)이 사람에게도 존재함이 알려져 큰 관심을 끌도 있는데 이중 UCP3는 그 발현이 골격근세포와 갈색지방조직에만 국한된다. 본 연구에서는 UCP3가 체내 인슐린 감수성을 결정하는데 가장 중요한 조직인 골격근에 국한되어 발현되는 점에 착안하여 UCP3를 골격근세포에 과발현시켰을 때 포도당 대사에 어떠한 영향이 나타나는 지를 조사하였다. 방법:25주령의 8마리의 OLETF 백서를 대상으로 하여 4마리는 골격근에 adenovirus 2mL(1×10¹²pfu/mL)를 주사하여 대조군으로 하였고 4마리는 골격근에 재조합법으로 제작된 adenovirus­UCP3 2mL(1×10¹²pfu/mL)를 주사하였다(UCP3 과발현군). UCP3를 투여한 백서에서 먹이섭취가 증가하는 경향이 있어 그 전날 대조군이 먹은 야의 먹이만큼 투여하였다. 골격근에 adenovirus를 주사한 10일 후에 euglycemic hyperinsulinemic clamp를 시행하였다. Adenovirus­UCP를 C2C12 골격근 세포에 transfection시켜 UCP3를 C2C12 골격근 세포에 transfection시켜 UPS3­C2C12를 만들고 C2C12 골격근 세포와 UPS3­C2C12 골격근 세포에서 포도당 수송 및 당원합성을 측정하였다. 결과:UCP3 과발현 OLETF에서 체중이 감소하는 경향을 보였고 인슐린 감수성이 증가하였다. C2C12세포에서 기저상태 포도당 수송은 1.28±0.17μmol/L/min였고 100nM 인슐린으로 2시간 처리한 후 2.67±0.20 μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 포도당 수송이 3.98±0.13μmol/L/min로 증가되었고 인슐린 처리 후 5.74±0.44μmol/L/min로 증가하였다. 인슐린을 처리한 UCP3­C2C12 세포에 P13K 억제제인 wortmannin을 첨가하였을 때 포도당 수송활성이 3.81±0.20μmol/L/min로 감소하였다. 기저상태 당원합성은 C2C12 세포에서 0.25±0.01μmol/L/min였고 인슐린 처리 후 0.45±0.01μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 당원합성이 0.62±0.01μmol/L/min였고 인슐린 처리 후 1.26±454μmol/L/min로 증가하였다. UCP3­C2C12세포에 wortmannin을 첨가하였을 때 당원합성율이 0.80±0.04μmol/L/min로 감소하였다. 결론:UCP3 과발현이 OLETF 백서에서 인슐린 감수성을 증가시켰고 골격근세포에서 포도당 수송 및 당원합성을 증가시켰다. wortmannin을 첨가하였을 때 포도당 수송 및 당원합성이 감소함으로 보아 이 과정이 인슐린 신호전달체계인 P13K에 일부 의존함을 알 수 있었다. Background : UC P3 is a mitochondrial membrane protein expressed selectively in the skeletal muscle and brown adipose tissue. Since the skeletal muscle is the main organ determining insulin sensitivity in the body, it was hypothesized that UCP3 overexpression in skeletal muscle cells would improve glucose metabolism. Methods : An adenovirus-UCP3 was produced by a recombinant DNA method. OLETF rats were divided into 2 groups. Four rats were injected with the adenovirus-UCP3 (UCP3 group) and others were injected with the adenovirus(control group) in the skeletal muscle. The UCP3 group was provided with the same quantity of food as that consumed by the control group on the previous day. Insulin sensitivity was evaluated by the euglycemic hyperinsulinemic clamp method. In a separate experiment, glucose transport and glycogen synthesis we evaluated in C2C212 cells transfected with ether an adenovirus or the adenovirus-UCP3. Results : The insulin sensitivity improved significantly and the body weight decreased in the UCP3 group. The glucose transport and glycogen synthesis were higher in the UCP3-C2C12 skeletal muscle cells at the basal state. After insulin treatment, glucose transport and glycogen synthesis were also higher in the UCP3-C2C12 cells but the increments were reduced after treatment with wortmannin, a PI3K inhibitor. Conclusion : Insulin sensitivity was higher in the UCP3-overexpressed OLETF rats in the in vivo study. UCP3 transfection also increased glucose transport and glycogen synthesis in the cultured skeletal muscle cells by a PI3K dependent mechanism(J Kor Diabetes Asso 25 :460~468, 2001).

SEM용 전자 검출기의 설계 및 제작

이상욱,전종업,박기태,박규열 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

The nature of the signals collected by an SEM(Scanning Electron Microscope) in order to form images are all dependent on the detector used to collect them, and the quality of an acquired image is strongly influenced by detector performance. Therefore, the development of detector with high performance is very important in pulling up the resolution of SEM This study presents the secondary electron detector for use in scanning electron microscope, electric circuit and I/V conversion circuit for driving that detector.

Review : Clinical applications of transient elastography

( Kyu Sik Jung ),( Seung Up Kim ) 대한간학회 2012 Clinical and Molecular Hepatology(대한간학회지) Vol.18 No.2

Chronic liver disease represents a major public health problem, accounting for significant morbidity and mortality worldwide. As prognosis and management depend mainly on the amount and progression of liver fibrosis, accurate quantifi cation of liver fi brosis is essential for therapeutic decision-making and follow-up of chronic liver diseases. Even though liver biopsy is the gold standard for evaluation of liver fi brosis, non-invasive methods that could substitute for invasive procedures have been investigated during past decades. Transient elastography (TE, FibroScan(R)) is a novel non-invasive method for assessment of liver fi brosis with chronic liver disease. TE can be performed in the outpatient clinic with immediate results and excellent reproducibility. Its diagnostic accuracy for assessment of liver fibrosis has been demonstrated in patients with chronic viral hepatitis; as a result, unnecessary liver biopsy could be avoided in some patients. Moreover, due to its excellent patient acceptance, TE could be used for monitoring disease progression or predicting development of liver-related complications. This review aims at discussing the usefulness of TE in clinical practice. (Clin Mol Hepatol 2012;18:163-173)

에폭시 수지(樹脂) 모르터의 강도(强度) 특성(特性)

연규석 ( Kyu Seok Yeon ),강신업 ( Sin Up Kang ) 한국농공학회 1982 韓國農工學會誌 : 전원과 자원 Vol.24 No.3

The objective of this study was to investigate the compressive and bending strength characteristics of epoxy resin mortar, which is still in an early stage of its use and study in Korea. The results obtained are summarized as follows; 1. The compressive strengths of epoxy resin mortar after 1 day, 2 days and 3 days were gained 87%, 91% and 95%, respectively, in view of that of mortar at the age of 7 days. This result showed that the initial compressive strength within 1 day was very high. 2. The highest compressive strength of epoxy resin mortar was 914 kg/cm² at the point of having the mixing ratio of one to two. It reached up to 3.7 times that of the normal portland cement mortar at the age of 28 days. 3. The bending strengths of epoxy resin mortar after 1 day, 2 days and 3 days came up to 88%, 93% and 97%, respectively, in comparing that of mortar at the age of 7 days. It was expressed to be simielar to the tendency of compressive strength. 4. The highest bending strength of epoxy resin mortar was 384 kg/cm2 at mixing ratio of one to two. It came up to as much as 6.5 times in comparing with that of the normal portland cement mortar at the age of 28 days. Therefore, the epoxy resin mortar would be effective for promoting the bending strength of structural members. 5. The regression equation between compressive and bending strength was obtained as follows; And the estimated value of bending strength was corresponded to about 44 per cent in comparing with that of the compressive strength.

에폭시 수지(樹脂) 모르터의 접착성(接着性) 및 내충격성(耐衝擊性)에 관한 연구(硏究)

연규석 ( Kyu Seok Yeon ),강신업 ( Sin Up Kang ) 한국농공학회 1983 韓國農工學會誌 : 전원과 자원 Vol.25 No.1

The purpose of resistance of epoxy this study was to investigate the bond strength and the impact resin mortar depending on the mixing ratio. The epoxy resin used in this study was to be Epi-Bis type resin. The test results to the extent of this report are summarized as follows: 1. The highest bond strength of epoxy resin mortar to the steel plate specimens was marked to be 12.9kg/㎠ at the mixing ratio of 1:2. It reached up to 5.8 times that of the plain cement mortar. The suitable mixing ratio for bonding of steel plate would be rich mix above 1:4. 2. The highest bond strength of epoxy resin mortar to the plain cement concrete was 9.1kg/㎠ at the mixing ratio of 1:2 and 1:4. By the way, the failure came into being at the concrete which is composed specimen. It was confirmed that the suitable mixing ratio for bonding of plain concrete would be rich mix above 1:6. 3. The impact resistance of flat specimens and cylinderic specimens came up to 7.5 times and 6.0 times, respectively, in comparing with that of plain cement mortar at the mixing ratio of 1:2. This result showed that the impact resistance of epoxy resin mortar was superior to plain cement mortar. 4. The highest bending impact resistance of square pillar specimen was 259.7kg·cm/㎠ at the mixing ratio of 1:2. It came up to 53 times in contrast with plain cement mortar. The exponential equation between bending strength(x) and bending impact strength(y) was obtained as follows; y=3. 34×1.0112^x (r=0.994) 5. Even if epoxy resin mortar belong to one and the same mixing ratio, the test results bring on a change according to the test method. And so, it is absolutly necessary for a worthy design to study on the suatable test method of the bond strength and impact resistance.

열(熱)을 받은 에폭시 수지(樹枝) 모르터의 강도(强度)에 관한 연구(硏究)

연규석 ( Kyu Seok Yeon ),강신업 ( Sin Up Kang ) 한국농공학회 1982 韓國農工學會誌 : 전원과 자원 Vol.24 No.4

The major objective of this study was to investigate the heat resistance of epoxy resin mortar. For this purpose, these tests of compressive and bending strength were accomp-olished with various heating temperature (40˚C, 60˚C, 80˚C, 100˚C, 120˚C), and with various mixing ratio (1: 2, 1: 4, 1: 6, 1: 8, 1:10, 1:12, 1:14). The exprimental resin was to be Epi-Bis type epoxy resin, which is widely used as construction materials. The results obtained are summarized as follows; 1. The variations of color tone started to begin at 60˚C, and it has come out very heavy at 120˚C. It was assumed that the decrement of weight resulted from carbonization were about 0.22% at 100˚C, and about 0.34% at 120˚C. 2. The compressive and bending strength were increased with temperature rise up to 80˚C, but these were made rapid decrease when the given temperature was over. And so, the mean decrement of compressive and bending strength at 120˚C reached up to 35.5% and 26.4%, respectively. 3. The regression equation between compressive and bending strength for epoxy resin mortar under heat exposure were obtained as follows; And the estimated value of bending strength was corresponded to about 37 percent in comparing with that of the compressive strength. 4. Consquently, the heat resistance temperature of epoxy resin mortar was to be around 80˚C, and it was generally very low values. But it was regarded that the epoxy resin mortar will not be difficult with materials of civil engineering works and agricultural structures.

Variable Association between Components of the Metabolic Syndrome and Electrocardiographic Abnormalities in Korean Adults

( Hong Kyu Kim ),( Chul Hee Kim ),( Kwan Ho Ko ),( Seong Wook Park ),( Joong Yeol Park ),( Ki Up Lee ) 대한내과학회 2010 The Korean Journal of Internal Medicine Vol.25 No.2

Background/Aims: Resting electrocardiogram (ECG) abnormalities have been strongly associated with cardiovascular disease mortality. Little is known, however, about the association between individual components of metabolic syndrome and ECG abnormalities, especially in Asian populations. Methods: We examined clinical and laboratory data from 31,399 subjects (age 20 to 89 years) who underwent medical check-ups. ECG abnormalities were divided into minor and major abnormalities based on Novacode criteria. Ischemic ECG findings were separately identified and analyzed. Results: The overall prevalence rates of ECG abnormalities were significantly higher in subjects with than in those without metabolic syndrome (p<0.01). Ischemic ECG was strongly associated with metabolic syndrome in all age groups of both sexes, except for younger women. In multiple logistic regression analysis, metabolic syndrome was independently associated with ischemic ECG (odds ratio, 2.30 [2.04 to 2.62]; p<0.01), after adjusting for sex, age, smoking, and family history of cardiovascular disease. Of the metabolic syndrome components, hyperglycemia in younger subjects and hypertension in elderly subjects were major factors for ischemic ECG changes, whereas hypertriglyceridemia was not an independent risk factor in any age group. The association between ischemic ECG findings and central obesity was weaker in women than in men. Conclusions: Metabolic syndrome was strongly associated with ECG abnormalities, especially ischemic ECG findings, in Koreans. The association between each component of metabolic syndrome and ECG abnormalities varied according to age and sex. (Korean J Intern Med 2010;25:174-180)

유럽 CO₂ 감축법에 따른 차량 CO₂ 감축 기술 전략에 관한 연구

석규업(Kyu-up Seok),윤형진(Hyoung Jin Yoon) 한국자동차공학회 2014 한국자동차공학회 학술대회 및 전시회 Vol.2014 No.11

The main purpose of this paper is to suggest opportunities for reducing CO₂ emission in energy conversion of a vehicle, focused on auxiliary energy improvement in the automotive field. As part of worldwide efforts to curb global warming and to protect the domestic industry as trade barriers, many countries have set goals to regulate greenhouse gas emissions. As an example, new CO₂ emission regulation in EU was expected to go into effect strictly in 2020. Therefore, global car makers need to establish strategic responsiveness of the regulations. This paper shows CO₂ economic value by using the correct interpretation of the relevant laws and regulations. The CO₂ value analyzed using quantitative figures leads to the possibility of auxiliary (accessories, HVAC, electric apparatus etc.) technology for improving fuel economy. As a result, this study generalizes the meaning of electric power saving for each driving mode by auxiliary energy improvement.

유럽 CO₂ 감축법에 따른 차량 CO₂ 감축 기술 전략에 관한 연구

석규업(Kyu-up Seok),윤형진(Hyoung Jin Yoon) 한국자동차공학회 2015 한국 자동차공학회논문집 Vol.23 No.2